Electron Beam-Supported Fabrication of Biocompatible Silver/iota-Carrageenan for Wound Healing Application.

Silver nanoparticles (AgNPs) are a potent antibacterial agent, especially when used to treat bacteria that are multidrug resistant. However, it is challenging to eliminate the hazardous reducing agents that remain in AgNPs produced by the conventional chemical reduction process. To overcome these challenges, the presented research demonstrates the fabrication of AgNPs using iota-carrageenan (ι-carra) as a carbohydrate polymer using electron beam (EB) irradiation. Well-characterized ι-carra@AgNPs have a face-centered cubic (FCC) structure with spherical morphology and an average size of 26 nm. Herein we explored the approach for fabricating ι-carra@AgNPs that is suitable for scaling up the production of nanoparticles that exhibit excellent water stability. Further, the optimized ι-carra@AgNPs exhibited considerable antibacterial activity of 40% and 30% inhibition when tested with Gram-negative Escherichia coli ATCC 43895 and Gram-positive Staphylococcus aureus (S. aureus) (ATCC 6538), respectively, and low cytotoxicity at 10-50 µg/mL. To establish the potential biomedical application, as proof of the concept, the ι-carra@AgNPs showed significant antibiofilm activity at 20 µg/mL and also showed 95% wound healing abilities at 50 µg/mL compared to the nontreated control groups. Electron beam assisted ι-carra@AgNPs showed significant beneficial effects against specific bacterial strains and may provide a guide for the development of new antibacterial materials for wound dressing for large-scale production for biomedical applications.

Sustainable utilization of fruit and vegetable waste bioresources for bioplastics production.

Nowadays, rapidly increasing production, use and disposable of plastic products has become one of the utmost environmental issues. Our current circumstances in which the food supply chain is demonstrated as containing plastic particles and other plastic-based impurities, represents a significant health risk to humans, animals, and environmental alike. According to this point of view, biodegradable plastic material aims to produce a more sustainable and greener world with a lower ecological impact. Bioplastics are being investigated as an environmentally friendly candidate to address this problem and hence global bioplastic production has seen significant growth and expansion in recent years. This article focuses on a few critical issues that must be addressed for bioplastic production to become commercially viable. Although the reduction of fruit and vegetable waste biomass has an apparent value in terms of environmental benefits and sustainability, commercial success at industrial scale has remained flat. This is due to various factors, including biomass feedstocks, pretreatment technologies, enzymatic hydrolysis, and scale-up issues in the industry, all of which contribute to high capital and operating costs. This review paper summarizes the global overview of bioplastics derived from fruit and vegetable waste biomass. Furthermore, economic and technical challenges associated with industrialization and diverse applications of bioplastics in biomedical, agricultural, and food-packaging fields due to their excellent biocompatibility properties are reviewed.HighlightsReview of the diverse types and characteristics of sustainability of biobased plasticsImproved pretreatment technologies can develop to enhance greater yieldEnzyme hydrolysis process used for bioplastic extraction & hasten industrial scale-upFocus on technical challenges facing commercialized the bioplasticsDetailed discussion on the application for sustainability of biodegradable plastics.

Metabolic engineering strategies to enable microbial electrosynthesis utilization of CO2: recent progress and challenges.

Microbial electrosynthesis (MES) is a promising technology that mainly utilizes microbial cells to convert CO2 into value-added chemicals using electrons provided by the cathode. However, the low electron transfer rate is a solid bottleneck hindering the further application of MES. Thus, as an effective strategy, genetic tools play a key role in MES for enhancing the electron transfer rate and diversity of production. We describe a set of genetic strategies based on fundamental characteristics and current successes and discuss their functional mechanisms in driving microbial electrocatalytic reactions to fully comprehend the roles and uses of genetic tools in MES. This paper also analyzes the process of nanomaterial application in extracellular electron transfer (EET). It provides a technique that combines nanomaterials and genetic tools to increase MES efficiency, because nanoparticles have a role in the production of functional genes in EET although genetic tools can subvert MES, it still has issues with difficult transformation and low expression levels. Genetic tools remain one of the most promising future strategies for advancing the MES process despite these challenges.

Microbial assisted multifaceted amelioration processes of heavy-metal remediation: a clean perspective toward sustainable and greener future.

Rapidly increasing heavy metal waste has adversely affected the environment and the Earth's health. The lack of appropriate remediation technologies has worsened the issue globally, especially in developing countries. Heavy-metals contaminants have severely impacted the environment and led to devastating conditions owing to their abundance and reactivity. As they are nondegradable, the potential risk increases even at a low concentration. However, heavy-metal remediation has increased with the up-gradation of technologies and integration of new approaches. Also, of all the treatment methodologies, microbial-assisted multifaceted approach for ameliorating heavy metals is a promising strategy for propagating the idea of a green and sustainable environment with minimal waste aggregation. Microbial remediation combined with different biotechniques could aid in unraveling new methods for eradicating heavy metals. Thus, the present review focuses on various microbial remediation approaches and their affecting factors, enabling recapitulation of the interplay between heavy-metals ions and microorganisms. Additionally, heavy-metals remediation mechanisms adapted by microorganisms, the role of genetically modified (GM) microorganisms, life cycle assessment (LCA), techno-economic assessment (TEA) limitations, and prospects of microbial-assisted amelioration of heavy-metals have been elaborated in the current review with focus toward "sustainable and greener future."

In silico analysis to identify potential antitubercular molecules in Morus alba through virtual screening and molecular dynamics simulations.

A major obstacle in the treatment of tuberculosis (TB) is to combat the emerging resistant strains of its causing agent i.e. Mycobacterium tuberculosis (MTb). The emergence of multidrug-resistant and extensively drug-resistant -TB strains raise a requirement of new potential anti-tubercular compounds. In this direction, different plant parts of Morus alba were tested against MTb and found to be active with a minimum inhibitory concentration ranging between 125 µg/ml to 31.5 µg/ml. Further to identify the phytochompounds having anti-mycobacterium activity, phytocompounds of the plant were docked against the five MTb proteins (PDB ID: 3HEM, 4OTK, 2QO0, 2AQ1 and 6MNA). Among twenty-two tested phytocompounds, four phytocompounds with effective binding energy (kcal/mol): Petunidin-3-rutinoside (3HEM: -8.2, 4OTK: -6.9, 2QO0: -9.0, 2AQ1: -8.3 and 6MNA:-7.8), Quercetin-3'-glucoside (3HEM:-6.7, 4OTK:-7.6, 2QO0:-7.6, 2AQ1:7.6 and 6MNA:-6.4), Rutin (3HEM:-7.8, 4OTK:-7.5, 2QO0:-9.1, 2AQ1:9.3 and 6MNA:-6.9) and Isoquercitrin (3HEM:-7.3, 4OTK:-6.6, 2QO0:-7.7, 2AQ1:8.3 and 6MNA:-6.6) shows promising activity against all the five target proteins. Further molecular dynamics studies of Petunidin-3-rutinoside with three target proteins 3HEM, 2AQ1 and 2QO0 resulted with low values of average RMSD (3.723 Å, 3.261 Å, and 2.497 Å, respectively) show that the complexes have better conformational stability. The wet lab validation of the current study will pave the new dimensions for the cure of TB patients.Communicated by Ramaswamy H. Sarma.

Cellulose-alginate hydrogels and their nanocomposites for water remediation and biomedical applications.

In the last decade, both cellulose and alginate polysaccharides have been extensively utilized for the synthesis of biocompatible hydrogels because of their alluring characteristics like low cost, biodegradability, hydrophilicity, biodegradability, ease of availability and non-toxicity. The presence of abundant hydrophilic functional groups (like carboxyl and hydroxyl) on the surface of cellulose and alginate or their derivatives makes these materials promising candidates for the preparation of hydrogels with appealing structures and characteristics, leading to growing research in water treatment and biomedical fields. These two polysaccharides are typically blended together to improve hydrogels' desired qualities (mechanical strength, adsorption properties, cellulose/alginate yield). So, keeping in view their extensive applicability, in the present review article, recent advances in the development of cellulose/nanocellulose-alginate-based hydrogels and their relevance in water treatment (adsorption of dyes, heavy metals, etc.) and biomedical field (wound healing, tissue engineering, drug delivery) has been reviewed. Further, impact of other inorganic/organic additives in cellulose/nanocellulose-alginate-based hydrogels properties like contaminants adsorption, drug delivery, tissue engineering, etc., has also been studied. Moreover, the current difficulties and future prospects of nanocellulose-alginate-based hydrogels regarding their water purification and biomedical applications are also discussed at the end.

Driving sustainable circular economy in electronics: A comprehensive review on environmental life cycle assessment of e-waste recycling.

E-waste, encompassing discarded materials from outdated electronic equipment, often ends up intermixed with municipal solid waste, leading to improper disposal through burial and incineration. This improper handling releases hazardous substances into water, soil, and air, posing significant risks to ecosystems and human health, ultimately entering the food chain and water supply. Formal e-waste recycling, guided by circular economy models and zero-discharge principles, offers potential solutions to this critical challenge. However, implementing a circular economy for e-waste management due to chemical and energy consumption may cause environmental impacts. Consequently, advanced sustainability assessment tools, such as Life Cycle Assessment (LCA), have been applied to investigate e-waste management strategies. While LCA is a standardized methodology, researchers have employed various routes for environmental assessment of different e-waste management methods. However, to the authors' knowledge, there lacks a comprehensive study focusing on LCA studies to discern the opportunities and limitations of this method in formal e-waste management strategies. Hence, this review aims to survey the existing literature on the LCA of e-waste management under a circular economy, shedding light on the current state of research, identifying research gaps, and proposing future research directions. It first explains various methods of managing e-waste in the circular economy. This review then evaluates and scrutinizes the LCA approach in implementing the circular bioeconomy for e-waste management. Finally, it proposes frameworks and procedures to enhance the applicability of the LCA method to future e-waste management research. The literature on the LCA of e-waste management reveals a wide variation in implementing LCA in formal e-waste management, resulting in diverse results and findings in this field. This paper underscores that LCA can pinpoint the environmental hotspots for various pathways of formal e-waste recycling, particularly focusing on metals. It can help address these concerns and achieve greater sustainability in e-waste recycling, especially in pyrometallurgical and hydrometallurgical pathways. The recovery of high-value metals is more environmentally justified compared to other metals. However, biometallurgical pathways remain limited in terms of environmental studies. Despite the potential for recycling e-waste into plastic or glass, there is a dearth of robust background in LCA studies within this sector. This review concludes that LCA can offer valuable insights for decision-making and policy processes on e-waste management, promoting environmentally sound e-waste recycling practices. However, the accuracy of LCA results in e-waste recycling, owing to data requirements, subjectivity, impact category weighting, and other factors, remains debatable, emphasizing the need for more uncertainty analysis in this field.

Date seed waste derived nanocatalyst and its application in production of hydrolytic enzyme, fermentative sugars and biohydrogen.

Biofuel production from cellulosic biomass is a promising approach; however, the cost-intensive utilization of cellulolytic enzymes is a major roadblock to economic production. This study reports the preparation of a nanocatalyst using date seed and evaluates the impact of nanocatalysts on cellulolytic enzyme production using solid-state fermentation of date pulp waste through bacterial co-cultivation. Under optimized conditions, 30 IU/gds filter paper activity is produced in the presence of 2 mg of nanocatalyst. Cellulase showed thermal stability at 50 °C and pH 7 up to 10 h in the presence of nanocatalyst, and it produced 32.31 g/L glucose through the hydrolysis of acidic-pretreated date seeds in 24 h. Subsequently, 1788 mL/L of cumulative H2 in 24 h through cocultured dark fermentation could be produced. The approach presented in this study can be effective for multiple value additions, including nanocatalyst preparation, cellulase enzyme, and biohydrogen production.

Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views.

Urbanization has driven the demand for fossil fuels, however, the overly exploited resource has caused severe damage on environmental pollution. Biorefining using abundant lignocellulosic biomass is an emerging strategy to replace traditional fossil fuels. Value-added lignin biomass reduces the waste pollution in the environment and provides a green path of conversion to obtain renewable resources. The technology is designed to produce biofuels, biomaterials and value-added products from lignocellulosic biomass. In the biorefinery process, the pretreatment step is required to reduce the recalcitrant structure of lignocellulose biomass and improve the enzymatic digestion. There is still a gap in the full and deep understanding of the biorefinery process including the pretreatment process, thus it is necessary to provide optimized and adapted biorefinery solutions to cope with the conversion process in different biorefineries to further provide efficiency in industrial applications. Current research progress on value-added applications of lignocellulosic biomass still stagnates at the biofuel phase, and there is a lack of comprehensive discussion of emerging potential applications. This review article explores the advantages, disadvantages and properties of pretreatment methods including physical, chemical, physico-chemical and biological pretreatment methods. Value-added bioproducts produced from lignocellulosic biomass were comprehensively evaluated in terms of encompassing biochemical products , cosmetics, pharmaceuticals, potent functional materials from cellulose and lignin, waste management alternatives, multifunctional carbon materials and eco-friendly products. This review article critically identifies research-related to sustainability of lignocellulosic biomass to promote the development of green chemistry and to facilitate the refinement of high-value, environmentally-friendly materials. In addition, to align commercialized practice of lignocellulosic biomass application towards the 21st century, this paper provides a comprehensive analysis of lignocellulosic biomass biorefining and the utilization of biorefinery green technologies is further analyzed as being considered sustainable, including having potential benefits in terms of environmental, economic and social impacts. This facilitates sustainability options for biorefinery processes by providing policy makers with intuitive evaluation and guidance.

Exosomal miRNAs as Next-generation Therapy Vehicles in Breast Cancer.

The second most pervasive cancer affecting the survival of women across the world is breast cancer. One of the biggest challenges in breast cancer treatment is the chemoresistance of cancer cells to various medications after some time. Therefore, highly specific blood-based biomarkers are required for early breast cancer diagnosis to overcome chemoresistance and improve patient survival. These days, exosomal miRNAs have attracted much attention as early diagnostic blood-based biomarkers because of their high stability, secretion from malignant tumor cells, and excellent specificity for different breast cancer subtypes. In addition, exosomal miRNAs regulate cell proliferation, invasion, metastasis, and apoptosis by binding to the 3'UTR of their target genes and limiting their production. This review focuses on the functions of exosomal miRNAs in tumorigenesis via targeting multiple signaling pathways as well as chemosensitivity and resistance mechanisms. In addition, the growing pieces of evidence discussed in this review suggest that circulating exosomal miRNAs could be utilized as potential next-generation therapeutic target vehicles in the treatment of breast cancer.

Recent advances in microbial engineering approaches for wastewater treatment: a review.

In the present era of global climate change, the scarcity of potable water is increasing both due to natural and anthropogenic causes. Water is the elixir of life, and its usage has risen significantly due to escalating economic activities, widespread urbanization, and industrialization. The increasing water scarcity and rising contamination have compelled, scientists and researchers, to adopt feasible and sustainable wastewater treatment methods in meeting the growing demand for freshwater. Presently, various waste treatment technologies are adopted across the globe, such as physical, chemical, and biological treatment processes. There is a need to replace these technologies with sustainable and green technology that encourages the use of microorganisms since they have proven to be more effective in water treatment processes. The present review article is focused on demonstrating how effectively various microbes can be used in wastewater treatment to achieve environmental sustainability and economic feasibility. The microbial consortium used for water treatment offers many advantages over pure culture. There is an urgent need to develop hybrid treatment technology for the effective remediation of various organic and inorganic pollutants from wastewater.

Microbial engineering approaches for wastewater treatment.Current and emerging sources of water pollution are discussed.Various treatment technologies for wastewater treatment.Biological methods and microbes are used for degradation.Parameters responsible for the degradations processes of wastewater.

Protein-polysaccharide nanoconjugates: Potential tools for delivery of plant-derived nutraceuticals.

Protein-polysaccharide nanoconjugates are covalently interactive networks that are currently the subject of intense research owing to their emerging applications in the food nanotechnology field. Due to their biocompatibility and biodegradability properties, they have played a significant role as wall materials for the formation of various nanostructures to encapsulate nutraceuticals. The food-grade protein-polysaccharide nanoconjugates would be employed to enhance the delivery and stability of nutraceuticals for their real use in the food industry. The most common edible polysaccharides (cellulose, chitosan, pectin, starch, carrageenan, fucoidan, mannan, glucomannan, and arabic gum) and proteins (silk fibroin, collagen, gelatin, soy protein, corn zein, and wheat gluten) have been used as potential building blocks in nano-encapsulation systems because of their excellent physicochemical properties. This article broadens the discussion of food-grade proteins and polysaccharides as nano-encapsulation biomaterials and their fabrication methods, along with a review of the applications of protein-polysaccharide nanoconjugates in the delivery of plant-derived nutraceuticals.

Facile pretreatment strategies to biotransform Kans grass into nanocatalyst, cellulolytic enzymes, and fermentable sugars towards sustainable biorefinery applications.

The present investigation is targeted towards the facile fabrication of a carbon-based nanocatalyst (CNCs) using Kans grass biomass (KGB) and its sustainable application in microbial cellulase enhancement for the alleviation of enzymatic hydrolysis for sugar production. Different pretreatments, including physical, KGB extract-mediated treatment, followed by KOH pretreatment, have been applied to produce CNCs using KGB. The presence of CNCs influences the pretreatment of KGB substrate, fungal cellulase production, stability, and sugar recovery in the enzymatic hydrolysis of KGB. Using 1.0% CNCs pretreated KGB-based solid-state fermentation, 33 U/gds FPA and 126 U/gds BGL were obtained at 72 h, followed by 107 U/gds EG at 48 h in the presence of 0.5% CNCs. Further, 42 °C has been identified as the optimum temperature for cellulase production, while the enzyme showed thermal stability at 50 °C up to 20 h and produced 38.4 g/L sugar in 24 h through enzymatic hydrolysis of KGB.

Integrated pretreatment of poplar biomass employing p-toluenesulfonic acid catalyzed liquid hot water and short-time ball milling for complete conversion to xylooligosaccharides, glucose, and native-like lignin.

This work aimed to study an integrated pretreatment technology employing p-toluenesulfonic acid (TsOH)-catalyzed liquid hot water (LHW) and short-time ball milling for the complete conversion of poplar biomass to xylooligosaccharides (XOS), glucose, and native-like lignin. The optimized TsOH-catalyzed LHW pretreatment solubilized 98.5% of hemicellulose at 160 °C for 40 min, releasing 49.8% XOS. Moreover, subsequent ball milling (20 min) maximized the enzymatic hydrolysis of cellulose from 65.8% to 96.5%, owing to the reduced particle sizes and cellulose crystallinity index. The combined pretreatment reduced the crystallinity by 70.9% while enlarging the average pore size and pore volume of the substrate by 29.5% and 52.4%, respectively. The residual lignin after enzymatic hydrolysis was rich in ß-O-4 linkages (55.7/100 Ar) with less condensed structures. This lignin exhibited excellent antioxidant activity (RSI of 66.22) and ultraviolet absorbance. Thus, this research suggested a sustainable waste-free biorefinery for the holistic valorization of biomass through two-step biomass fractionation.

Current and emerging applications of saccharide-modified chitosan: a critical review.

Chitin, as the main component of the exoskeleton of Arthropoda, is a highly available natural polymer that can be processed into various value-added products. Its most important derivative, i.e., chitosan, comprising ß-1,4-linked 2-amino-2-deoxy-ß-d-glucose (deacetylated d-glucosamine) and N-acetyl-d-glucosamine units, can be prepared via alkaline deacetylation process. Chitosan has been used as a biodegradable, biocompatible, non-antigenic, and nontoxic polymer in some in-vitro applications, but the recently found potentials of chitosan for in-vivo applications based on its biological activities, especially antimicrobial, antioxidant, and anticancer activities, have upgraded the chitosan roles in biomaterials. Chitosan approval, generally recognized as a safe compound by the United States Food and Drug Administration, has attracted much attention toward its possible applications in diverse fields, especially biomedicine and agriculture. Despite some favorable characteristics, the chitosan's structure should be customized for advanced applications, especially due to its drawbacks, such as low drug-load capacity, low solubility, high viscosity, lack of elastic properties, and pH sensitivity. In this context, derivatization with relatively inexpensive and highly available mono- and di-saccharides to soluble branched chitosan has been considered a "game changer". This review critically scrutinizes the emerging technologies based on the synthesis and application of lactose- and galactose-modified chitosan as two important chitosan derivatives. Some characteristics of chitosan derivatives and biological activities have been detailed first to understand the value of these natural polymers. Second, the saccharide modification of chitosan has been discussed briefly. Finally, the applications of lactose- and galactose-modified chitosan have been scrutinized and compared to native chitosan to provide an insight into the current state-of-the research for stimulating new ideas with the potential of filling research gaps.

A global perspective on a new paradigm shift in bio-based meat alternatives for healthy diet.

A meat analogue is a casserole in which the primary ingredient is something other than meat. It goes by various other names, such as meat substitute, fake meat, alternative meat, and imitation meat. Consumers growing interest in improving their diets and the future of the planet have contributed to the move towards meat substitutes. This change is due to the growing popularity of low-fat and low-calorie diets, the rise of flexitarians, the spread of animal diseases, the loss of natural resources, and the need to cut down on carbon emissions, which lead to greenhouse effects. Plant-based meat, cultured meat, algal protein-based meat, and insect-based meat substitutes are available on the market with qualities like appearance and flavor similar to those of traditional meat. Novel ingredients like mycoprotein and soybean leg haemoglobin are mixed in with the more traditional soy proteins, cereals, green peas, etc. Plant-based meat is currently more popular in the West, but the growing interest in this product in Asian markets indicates the industry in this region will expand rapidly in the near future. Future growth in the food sector can be anticipated from technologies like lab-grown meat and its equivalents that do not require livestock breeding. Insect-based products also hold great potential as a new source of protein for human consumption. However, product safety and quality should be considered along with other factors such as marketability and affordability.

Potential application of polysaccharide mucilages as a substitute for emulsifiers: A review.

Mucilages are natural compounds consisting mainly of polysaccharides with complex chemical structures. Mucilages also contain uronic acids, proteins, lipids, and bioactive compounds. Because of their unique properties, mucilages are used in various industries, including food, cosmetics, and pharmaceuticals. Typically, commercial gums are composed only of polysaccharides, which increase their hydrophilicity and surface tension, reducing their emulsifying ability. As a result of the presence of proteins in combination with polysaccharides, mucilages possess unique emulsifying properties due to their ability to reduce surface tension. In recent years, various studies have been conducted on using mucilages as emulsifiers in classical and Pickering emulsions because of their unique emulsifying feature. Studies have shown that some mucilages, such as yellow mustard, mutamba, and flaxseed mucilages, have a higher emulsifying capacity than commercial gums. A synergistic effect has also been shown in some mucilages, such as Dioscorea opposita mucilage when combined with commercial gums. This review article investigates whether mucilages can be used as emulsifiers and what factors affect their emulsifying properties. A discussion of the challenges and prospects of using mucilages as emulsifiers is also presented in this review.