ABSTRACT
BACKGROUND: Exposure to aerosolized harmless antigen such as ovalbumin (OVA) has previously been shown to induce inhalation tolerance, a state characterized by inhibition of IgE synthesis and airway inflammation, upon secondary immunogenic antigen encounter. Immune events associated with this phenomenon are still poorly understood. OBJECTIVE: The aim of this study was to investigate cellular and molecular mechanisms underlying this state of 'unresponsiveness'. METHODS: After initial repeated OVA exposure, mice were subjected to a protocol of antigen-induced airway inflammation, encompassing two intraperitoneal injections of OVA adsorbed to aluminium hydroxide followed by airway challenge. We assessed immune events in the draining lymph nodes after sensitization, and in the lungs after challenge. RESULTS: In animals initially exposed to OVA, we observed, at the time of sensitization, considerable expansion of T cells, many of which expressed the activation markers CD69 and CD25, as well as increased numbers of antigen-presenting cells, particularly B cells. While these animals produced low levels of IgE, the observed elevated levels of IgG1 signified isotype switching. Splenocytes and lymph node cells from OVA-exposed mice produced low levels of IL-4, IL-5, IL-13 and IFN-gamma, indicating aborted effector function of both T helper (Th)2- and Th1-associated cytokines. Real time quantitative polymerase chain reaction (PCR) (TaqMan) analysis of costimulatory molecules in the lungs after in vivo challenge showed that B7.1, B7.2, CD28 and CTLA-4 mRNA expression was low in animals initially exposed to OVA. Ultimately, these events were associated with abrogated airway inflammation and attenuated airway hyper-responsiveness. The decreased inflammation was antigen-specific and independent of IL-10 or IFN-gamma. CONCLUSION: Initial exposure to OVA establishes a programme that prevents the generation of intact, fully functional inflammatory responses upon secondary antigen encounter. The absence of inflammation, however, is not associated with categorical immune unresponsiveness.
Subject(s)
Cytokines/drug effects , Cytokines/immunology , Immunoglobulins/drug effects , Immunoglobulins/immunology , Inhalation Exposure/adverse effects , Mice, Inbred BALB C/immunology , Ovalbumin/immunology , Ovalbumin/pharmacology , Animals , Antibody Specificity/drug effects , Antibody Specificity/immunology , Biomarkers/blood , Bronchial Provocation Tests , Bronchoalveolar Lavage Fluid/chemistry , Bronchoalveolar Lavage Fluid/immunology , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , CD8-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/metabolism , Cytokines/blood , Dose-Response Relationship, Immunologic , Female , Histocompatibility Antigens Class II/drug effects , Histocompatibility Antigens Class II/immunology , Immunoglobulins/blood , Leukocytes, Mononuclear/drug effects , Leukocytes, Mononuclear/metabolism , Lung/blood supply , Lung/cytology , Mice , Mice, Knockout , Models, Animal , RNA, Messenger/biosynthesis , RNA, Messenger/drug effects , Sodium Chloride/pharmacology , Time FactorsABSTRACT
It is essential to understand the molecular basis of a host's response to microbial infection so that disease and tissue damage can be prevented. Modulation of host RNA expression is a critical set of molecular changes that occur upon infection. Global analysis of gene expression should provide an understanding of host RNA transcriptional changes that occur upon host-pathogen interaction. This series of articles focuses on the use of microarrays for analyzing the transcriptional response of a host to microbial infection and for drug target identification.
Subject(s)
Bacterial Infections/microbiology , Oligonucleotide Array Sequence Analysis , Virus Diseases/virology , Animals , Bacterial Infections/drug therapy , Drug Delivery Systems , Gene Expression Profiling , Humans , RNA/genetics , Transcription, Genetic , Virus Diseases/drug therapyABSTRACT
The objective of this study was to define phenotypic changes of antigen-presenting cells (APCs) and T cells in a murine model of antigen-induced airways inflammation that involves intraperitoneal sensitization with ovalbumin (OVA)/adjuvant followed by antigen aerosolization. We investigated the APC and T-cell compartments both after sensitization (primary immune response) and after challenge (secondary immune response) at the thoracic lymph nodes (initiation site) and the lung (effector site). Our findings document a major cellular expansion in the lymph nodes after both sensitization and challenge. After sensitization, this expansion was comprised mainly of B cells, a considerable proportion of which expressed B7.2. At this time, T cells were markedly expanded and activated as assessed by CD69 expression; further, although GATA-3 and signal transducer and activator of transcription-6 were expressed at this time point, expression of interleukin (IL)-4, IL-5, and IL-13 messenger RNA (mRNA) levels were marginal. However, in vitro stimulation of lymph-node cells with OVA led to cytokine production. In contrast, 24 h after challenge, but not after sensitization, there was a major expansion of dendritic cells and macrophages in the lungs. This expansion was associated with enhanced expression of both B7.1 and B7.2. We also observed expansion of activated CD3(+)/CD4(+) T cells expressing the T helper-2-associated marker T1/ST2 in the lung, most notably 5 d after challenge. Further, IL-4, IL-5, and IL-13, but not interferon-gamma mRNA were expressed at high levels 3 h after challenge. This study helps to elucidate the "geography" of immune responses generated in a conventional murine model of allergic airways inflammation.
Subject(s)
Antigen-Presenting Cells/immunology , Membrane Proteins , Ovalbumin/immunology , Pneumonia/immunology , T-Lymphocyte Subsets/immunology , Aerosols , Animals , Antigen-Presenting Cells/cytology , Antigen-Presenting Cells/metabolism , Antigens, CD/metabolism , B7-1 Antigen/metabolism , B7-2 Antigen , Bronchial Provocation Tests , Cytokines/genetics , Cytokines/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Disease Models, Animal , Female , Flow Cytometry , GATA3 Transcription Factor , Histocompatibility Antigens Class II/metabolism , Interleukin-1 Receptor-Like 1 Protein , Lung/cytology , Lymph Nodes/cytology , Membrane Glycoproteins/metabolism , Mice , Mice, Inbred BALB C , Ovalbumin/administration & dosage , Pneumonia/chemically induced , Proteins/genetics , Proteins/metabolism , Receptors, Interleukin , STAT6 Transcription Factor , T-Lymphocyte Subsets/metabolism , Trans-Activators/genetics , Trans-Activators/metabolismABSTRACT
The objective of this study was to investigate the contribution of secondary lymphoid organs in the generation and maintenance of experimental allergic airway inflammation. We employed a previously reported murine model of respiratory mucosal allergic sensitization, induced by repeated aerosolizations of ovalbumin in the context of a GM-CSF airway environment. We executed this protocol in wild-type (WT) and lymphotoxin-alpha-deficient mice (LTalpha-KO) mice, which are devoid of lymph nodes (LNs) and possess rudimentary spleen structures. Despite the lack of pulmonary LNs draining the airway compartment, LTalpha-KO mice were fully capable of mounting a robust inflammatory response in the airways, consisting of Th2 polarized CD4+ T cells and eosinophils. This was accompanied by IL-5, IL-13, and IFN-gamma production by splenocytes and generation of ovalbumin-specific serum IgE. Exposure to the same antigen 7 weeks after complete resolution of airway inflammation once again induced a Th2 polarized infiltrate, demonstrating intact immunological memory. To investigate inherent plasticity in establishing antigen-specific immunity, mice were splenectomized before sensitization. Allergic sensitization was completely abrogated in splenectomized LTalpha-KO mice, compared with eusplenic LTalpha-KO controls. These data demonstrate that secondary lymphoid organs, either LN or spleen, are essential for the generation of allergic airway responses.
Subject(s)
Disease Models, Animal , Lymph Nodes/immunology , Respiratory Hypersensitivity/immunology , Spleen/immunology , Administration, Intranasal , Animals , Bronchoalveolar Lavage Fluid , CD8-Positive T-Lymphocytes/immunology , Cells, Cultured , Eosinophils/immunology , Genetic Vectors/administration & dosage , Granulocyte-Macrophage Colony-Stimulating Factor/genetics , Granulocyte-Macrophage Colony-Stimulating Factor/physiology , Immunoglobulin E/biosynthesis , Immunologic Memory , Inflammation , Interferon-gamma/biosynthesis , Interleukin-13/biosynthesis , Interleukin-5/biosynthesis , Lymph Nodes/abnormalities , Lymphotoxin-alpha/genetics , Lymphotoxin-alpha/physiology , Mice , Mice, Inbred C57BL , Mice, Knockout , Ovalbumin/toxicity , Pulmonary Eosinophilia/etiology , Pulmonary Eosinophilia/immunology , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/physiology , Respiratory Hypersensitivity/etiology , Specific Pathogen-Free Organisms , Spleen/abnormalities , Spleen/cytology , Splenectomy , Th2 Cells/immunologyABSTRACT
Intercellular adhesion molecule 1 (ICAM-1) is a widely expressed glycoprotein involved in leukocyte extravasation and the interaction of lymphocytes with antigen-presenting cells. We examined these aspects of ICAM-1 function in the central nervous system after axonal injury in wild-type and ICAM-1-deficient mice. ICAM-1 immunoreactivity in the normal mouse facial nucleus was restricted to the vascular endothelium. Transection of the facial nerve led to a fast upregulation of ICAM-1 on activated microglia in the axotomized facial nucleus and the infiltration of ICAM-1-positive lymphocytes. Labeling elsewhere was unchanged. In homozygous ICAM-1 mutant mice, ICAM-1 was absent from endothelial cells and lymphocytes, but low levels of ICAM-1 were detected on cell membranes of reactive microglial cells. Comparison of wild-type animals with homozygously bred, ICAM-1-deficient mice showed a reduction of astrocytic and microglial activation, massive late axonal sprouting, and decreased lymphocyte infiltration. These experiments were repeated in F1 progeny of heterozygous mice on a C57BL/6 background. Neuroglial activation and lymphocyte infiltration in F1 homozygously deficient mice was unaffected compared with wild-type siblings. The invading ICAM-1-deficient lymphocytes also adhered to the ICAM-1-positive phagocytotic microglial cells in the ICAM-1 mutants. No change in the recruitment of macrophages and granulocytes into the crushed facial nerve, and no effect on axonal regeneration occurred. These data argue against the requirement of endothelial ICAM-1 in the recruitment of leukocytes into the crushed peripheral nerve or the axotomized facial motor nucleus and stress the importance of adequately matched controls in studying the effects of gene deletion in experimental animals.
Subject(s)
Astrocytes/cytology , Facial Nerve/physiology , Intercellular Adhesion Molecule-1/genetics , Leukocytes/cytology , Microglia/cytology , Nerve Regeneration/immunology , Animals , Axons/physiology , Axotomy , Breeding , Chemotaxis, Leukocyte , Exons , Facial Nerve/cytology , Female , Gene Expression/physiology , Heterozygote , Immunohistochemistry , Intercellular Adhesion Molecule-1/analysis , Laminin/analysis , Leukocytes/chemistry , Male , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Mice, Knockout , Motor Neurons/chemistry , Motor Neurons/ultrastructure , Nerve CrushABSTRACT
Fractalkine, the first member of the CX(3)C chemokine family, induces leukocyte chemotaxis through activation of its high affinity receptor, CX(3)CR1. Like other chemokine receptors, CX(3)CR1 is coupled to a pertussis toxin-sensitive heterotrimeric G(i) protein, which is necessary for rapid rise in the concentration of intracellular calcium. Using a Chinese hamster ovary cell line stably transfected with the CX(3)CR1 receptor, we show that the source of calcium mobilized by fractalkine stimulation is the extracellular pool. Calcium influx is blocked by extracellular calcium chelators, as well as by divalent heavy metals such as Ni(2+), Co(2+), and Cd(2+) without affecting the integrity of intracellular stores. Remarkably, selective phosphoinositide 3-kinase (PI3K) inhibitors, wortmannin and LY294002, abolish the wave extracellular calcium, suggesting that an active PI3K is necessary for this event. The influx of extracellular calcium is in turn required to trigger the activation of the p42/44 mitogen-activated protein/extracellular signal-regulated kinase pathway, but is not necessary for other signals downstream to PI3K, such as phosphorylation of Akt. The potential role of this signaling cascade in fractalkine-mediated chemotaxis is discussed.
Subject(s)
Calcium/metabolism , MAP Kinase Signaling System , Phosphatidylinositol 3-Kinases/metabolism , Receptors, Cytokine/metabolism , Receptors, HIV/metabolism , Androstadienes/pharmacology , Animals , CHO Cells , CX3C Chemokine Receptor 1 , Chromones/pharmacology , Cricetinae , Enzyme Inhibitors/pharmacology , Ion Transport , Morpholines/pharmacology , Phosphoinositide-3 Kinase Inhibitors , Reverse Transcriptase Polymerase Chain Reaction , Transfection , Type C Phospholipases/antagonists & inhibitors , WortmanninABSTRACT
Primary T cell activation requires B7-CD28 and CD40-CD154 costimulation, but effector T cell functions are considered to be largely independent of these costimulatory pathways. Although blockade of costimulation with cytolytic T lymphocyte-associated antigen 4-immunoglobulin (CTLA-4-Ig) or monoclonal antibody (mAb) to CD154 prolongs allograft survival, chronic rejection follows, which suggests that additional key costimulatory pathways are active in vivo. We found that both antibody to inducible costimulator (anti-ICOS) and an ICOS-Ig fusion protein suppressed intragraft T cell activation and cytokine expression and prolonged allograft survival in a manner similar to that in ICOS-/- allograft recipients. The combination of anti-ICOS therapy and cyclosporin A led to permanent engraftment. In addition, ICOS-B7RP-1 costimulation was required for the development of chronic rejection after CD40-CD154 blockade. These data demonstrate a key role for the ICOS-B7RP-1 pathway in acute and chronic rejection and highlight the benefits of targeting this pathway in combination with the use of conventional immunosuppressive agent.
Subject(s)
Antigens, Differentiation, T-Lymphocyte/immunology , B7-1 Antigen/immunology , Graft Rejection/immunology , Heart Transplantation/immunology , Animals , Antigens, Differentiation, T-Lymphocyte/genetics , CD40 Ligand/immunology , Cyclosporine/immunology , Cyclosporine/pharmacology , Gene Expression , Graft Survival/immunology , Immunosuppressive Agents/immunology , Immunosuppressive Agents/pharmacology , Inducible T-Cell Co-Stimulator Ligand , Inducible T-Cell Co-Stimulator Protein , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Transplantation, Homologous/immunology , Up-Regulation/immunologyABSTRACT
We examined the requirement for and cooperation between CD28 and inducible costimulator (ICOS) in effective T helper (TH) cell responses in vivo. We found that both CD28 and ICOS were critical in determining the outcome of an immune response; cytolytic T lymphocyte-associated antigen 4-immunoglobulin (CTLA-4-Ig), ICOS-Ig and/or a neutralizing ICOS monoclonal antibody attenuated T cell expansion, TH2 cytokine production and eosinophilic inflammation. CD28-dependent signaling was essential during priming, whereas ICOS-B7RP-1 regulated TH effector responses, and the up-regulation of chemokine receptors that determine T cell migration. Our data suggests a scenario whereby both molecules regulate the outcome of the immune response but play separate key roles: CD28 primes T cells and ICOS regulates effector responses.
Subject(s)
Antigens, Differentiation, T-Lymphocyte/immunology , Immunoconjugates , Lung/immunology , Th1 Cells/immunology , Th2 Cells/immunology , Abatacept , Animals , Antibodies, Monoclonal/biosynthesis , Antigens, CD , Antigens, Differentiation/genetics , Antigens, Differentiation, T-Lymphocyte/genetics , CD28 Antigens/genetics , CTLA-4 Antigen , Cytokines/biosynthesis , Gene Expression , Immunity, Mucosal/immunology , Immunoglobulin E/biosynthesis , Inducible T-Cell Co-Stimulator Protein , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Neutralization Tests , Rats , Rats, Inbred WKY , Receptors, CCR3 , Receptors, CCR4 , Receptors, CCR8 , Receptors, Chemokine/genetics , Respiratory Mucosa/immunologyABSTRACT
The inducible costimulatory molecule (ICOS) is expressed on activated T cells and participates in a variety of important immunoregulatory functions. After the induction of experimental allergic encephalomyelitis in SJL mice with proteolipid protein (PLP), brain ICOS mRNA and protein were up-regulated on infiltrating CD3+ T cells before disease onset. ICOS blockade during the efferent immune response (9-20 days after immunization) abrogated disease, but blockade during antigen priming (1-10 days after immunization) exacerbated disease. Upon culture with PLP and compared with immunized controls, splenocytes produced either decreased interferon-gamma (IFN-gamma, in efferent blockade) or excessive IFN-gamma (in priming blockade). PLP-specific immunoglobulin G1 was decreased in animals treated with anti-ICOS during antigen priming, but not in other groups.
Subject(s)
Antigens, Differentiation, T-Lymphocyte/immunology , Encephalomyelitis, Autoimmune, Experimental/immunology , Animals , Antigens, Differentiation, T-Lymphocyte/genetics , B7-1 Antigen/genetics , B7-1 Antigen/immunology , Brain/immunology , Brain/pathology , Cytokines/biosynthesis , Cytokines/genetics , Encephalomyelitis, Autoimmune, Experimental/pathology , Female , Immunoglobulin G/biosynthesis , Inducible T-Cell Co-Stimulator Ligand , Inducible T-Cell Co-Stimulator Protein , Interferon-gamma/biosynthesis , Mice , Myelin Proteolipid Protein/adverse effects , Myelin Proteolipid Protein/immunology , T-Lymphocytes/immunology , Up-Regulation/immunologyABSTRACT
VCAM-1 and ICAM-1 are endothelial adhesion molecules of the Ig gene superfamily that may participate in atherogenesis by promoting monocyte accumulation in the arterial intima. Both are expressed in regions predisposed to atherosclerosis and at the periphery of established lesions, while ICAM-1 is also expressed more broadly. To evaluate functions of VCAM-1 in chronic disease, we disrupted its fourth Ig domain, producing the murine Vcam1(D4D) allele. VCAM-1(D4D) mRNA and protein were reduced to 2-8% of wild-type allele (Vcam1(+)) levels but were sufficient to partially rescue the lethal phenotype of VCAM-1-null embryos. After crossing into the LDL receptor-null background, Vcam1(+/+) and Vcam1(D4D/D4D) paired littermates were generated from heterozygous intercrosses and fed a cholesterol-enriched diet for 8 weeks. The area of early atherosclerotic lesions in the aorta, quantified by en face oil red O staining, was reduced significantly in Vcam1(D4D/D4D) mice, although cholesterol levels, lipoprotein profiles, and numbers of circulating leukocytes were comparable to wild-type. In contrast, deficiency of ICAM-1 either alone or in combination with VCAM-1 deficiency did not alter nascent lesion formation. Therefore, although expression of both VCAM-1 and ICAM-1 is upregulated in atherosclerotic lesions, our data indicate that VCAM-1 plays a dominant role in the initiation of atherosclerosis.
Subject(s)
Arteriosclerosis/etiology , Intercellular Adhesion Molecule-1/metabolism , Vascular Cell Adhesion Molecule-1/metabolism , Animals , Aorta/pathology , Arteriosclerosis/genetics , Diet, Atherogenic , Embryo Loss , Intercellular Adhesion Molecule-1/genetics , Leukocyte Count , Mice , Mice, Mutant Strains , Mutation , Time Factors , Vascular Cell Adhesion Molecule-1/geneticsABSTRACT
T cells secreting interleukin (IL)-4 and IL-5 (T helper cell type 2 [Th2] cells) play a detrimental role in a variety of diseases, but specific methods of regulating their activity remain elusive. T1/ST2 is a surface ligand of the IL-1 receptor family, expressed on Th2- but not on interferon (IFN)-gamma-producing Th1 cells. Prior exposure of BALB/c mice to the attachment (G) or fusion (F) protein of respiratory syncytial virus (RSV) increases illness severity during intranasal RSV challenge, due to Th2-driven lung eosinophilia and exuberant Th1-driven pulmonary infiltration, respectively. We used these polar models of viral illness to study the recruitment of T1/ST2 cells to the lung and to test the effects of anti-T1/ST2 treatment in vivo. T1/ST2 was present on a subset of CD4(+) cells from mice with eosinophilic lung disease. Monoclonal anti-T1/ST2 treatment reduced lung inflammation and the severity of illness in mice with Th2 (but not Th1) immunopathology. These results show that inhibition of T1/ST2 has a specific effect on virally induced Th2 responses and suggests that therapy targeted at this receptor might be of value in treating Th2-driven illness.
Subject(s)
Membrane Proteins , Proteins/immunology , Receptors, Interleukin-1/immunology , Respiratory Syncytial Virus Infections/immunology , T-Lymphocytes, Helper-Inducer , Animals , Eosinophilia/immunology , Eosinophilia/therapy , Female , Interleukin-1 Receptor-Like 1 Protein , Mice , Mice, Inbred BALB C , Receptors, Interleukin , Respiratory Syncytial Virus Infections/therapy , Th1 Cells , Th2 CellsABSTRACT
Stromal cell-derived factor-1alpha (SDF-1alpha) is a potent chemoattractant for hematopoietic progenitor cells (HPC), suggesting that it could play an important role during their migration within or to the bone marrow (BM). The integrin VLA-4 mediates HPC adhesion to BM stroma by interacting with CS-1/fibronectin and VCAM-1. It is required during hematopoiesis and homing of HPC to the BM. As HPC migration in response to SDF-1alpha might require dynamic regulation of integrin function, we investigated if SDF-1alpha could modulate VLA-4 function on BM CD34(hi) cells.CD34(hi) BM cells and hematopoietic cell lines were tested for the effect of SDF-1alpha on VLA-4-dependent adhesion to CS-1/fibronectin and VCAM-1, as well as to BM stroma. CD34(hi) BM cells that adhered to VLA-4 ligands after SDF-1alpha treatment were characterized in colony-forming and long-term culture-initiating cell (LTC-IC) assays.SDF-1alpha rapidly (1 minute) and transiently upregulated the adhesion of CD34(hi) BM cells and hematopoietic cell lines to both CS-1/fibronectin and VCAM-1, and to BM stromal cells. The upregulation of VLA-4-dependent cell adhesion by SDF-1alpha targeted primitive LTC-IC as well as committed CD34(hi) cells. SDF-1alpha-triggered enhancement in VLA-4 function was inhibited by pertussis toxin (PTx) and cytochalasin D, indicating the involvement of G(i) protein downstream signaling and an intact cytoskeleton. Instead, activation of p44/42 MAP kinases by SDF-1alpha did not functionally correlate with enhancement of VLA-4-dependent cell adhesion. Modulation of VLA-4-mediated CD34(hi) BM cell adhesion by SDF-1alpha could play a key role in their migration within and to the BM and therefore influence their proliferation and differentiation.
Subject(s)
Cell Adhesion/drug effects , Chemokines, CXC/physiology , Fibronectins/metabolism , Hematopoietic Stem Cells/drug effects , Integrins/physiology , Peptide Fragments/metabolism , Receptors, Lymphocyte Homing/physiology , Vascular Cell Adhesion Molecule-1/metabolism , Animals , Antibodies, Monoclonal/pharmacology , Bone Marrow Cells/drug effects , Bone Marrow Cells/metabolism , Cell Line , Chemokine CXCL12 , Chemokines, CXC/pharmacology , Chemotaxis/drug effects , Colony-Forming Units Assay , Hematopoietic Cell Growth Factors/pharmacology , Hematopoietic Stem Cells/metabolism , Humans , Integrin alpha4beta1 , Leukemia, Megakaryoblastic, Acute/pathology , Liver/cytology , Liver/embryology , Mice , Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/pathology , Receptors, CXCR4/metabolism , Recombinant Proteins/pharmacology , Signal Transduction/drug effects , Stromal Cells/physiology , Tumor Cells, Cultured/drug effects , Tumor Cells, Cultured/metabolismABSTRACT
The orphan receptor T1/ST2, a member of the IL-1R family, is preferentially expressed on the surface of murine Th2 cells. In this study, we analyzed the kinetics and function of T1/ST2 expression on Th2 cells in vitro. Whereas naive CD4(+) cells did not express T1/ST2, most CD4(+) cells became T1/ST2(+) upon repeated antigenic stimulation under Th2-polarizing conditions. Flow cytometric analyses revealed that the kinetics of T1/ST2 expression on Th2 cells was delayed compared with the kinetics of type 2 cytokine production. Exogenous IL-6, IL-5, IL-1, and TNF-alpha enhanced the expression of T1/ST2 on Th2 cells, and IL-6 was by far most effective in this regard. However, the expression of T1/ST2 did not depend on the presence of IL-6 and was also detected in IL-6-deficient mice. Most important, cross-linking of T1/ST2 provided a costimulatory signal for Th2 but not Th1 cells and directly induced proliferation and type 2 cytokine production. Thus, T1/ST2 is not only a Th2 cell marker but also plays an important role in the activation of Th2 cells.
Subject(s)
Cytokines/biosynthesis , Membrane Proteins , Protein Biosynthesis , Proteins/immunology , Th2 Cells/immunology , Th2 Cells/metabolism , Animals , Antibodies, Monoclonal/metabolism , Cells, Cultured , Interleukin-1 Receptor-Like 1 Protein , Interleukin-4/biosynthesis , Interleukin-5/pharmacology , Interleukin-6/pharmacology , Kinetics , Lymphocyte Activation , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Transgenic , Muromonab-CD3/pharmacology , Proteins/metabolism , Proteins/physiology , Receptors, Interleukin , Tumor Necrosis Factor-alpha/pharmacology , Up-Regulation/immunologyABSTRACT
Upon encounter with specific antigen, naïveT helper precursor (THP) cells become activated. This event is regulated not only by engagement of the T cell receptor (TCR) with peptide presented in the context of major histocompatibility complex (MHC) class II molecules but by a number of costimulatory signals. CD28 engagement by B7-1 and B7-2 on resting THP cells provides a critical signal for initial cell cycle progression, interleukin 2 production and clonal expansion. However, largely as a consequence of the unraveling of the human genome, it is becoming clear that B7-1 and B7-2 are part of a larger family T of related counter-receptors that play an essential role in regulating the fate of primed, rather then resting,THP cells. These molecules play an important sequential role and act, together with B7-1- and B7-2-primed T cells, in the acquisition of effector function and/or tolerance induction.
Subject(s)
B7-1 Antigen/immunology , Immunoconjugates , Lymphocyte Activation , T-Lymphocytes, Helper-Inducer/immunology , Abatacept , Animals , Antigens, CD/genetics , Antigens, CD/immunology , Antigens, Differentiation/immunology , B7-1 Antigen/genetics , B7-2 Antigen , CD28 Antigens/immunology , CTLA-4 Antigen , Cytokines/biosynthesis , Forecasting , Humans , Inducible T-Cell Co-Stimulator Ligand , Lymphocyte Cooperation , Membrane Glycoproteins/genetics , Membrane Glycoproteins/immunology , Mice , Models, Immunological , Phylogeny , Protein Isoforms/genetics , Protein Isoforms/immunologyABSTRACT
We have used two models of murine pulmonary inflammation to investigate the signals responsible for the resolution of bronchial hyperresponsiveness (BHR). Both protocols involved two sensitizations with OVA followed by serial aerosolized challenge with OVA. We determined that administration of the second sensitization by aerosol (model A) was associated with a transient response, whereas administration by the i.p. route (model B) induced a sustained response, in the form of BHR and eosinophilia. This difference in kinetics was due solely to the route of the second Ag administration and was not associated with Ag dose or adjuvant. Differences in kinetics of lung eosinophilia/BHR were shown to be independent of IgE levels and IL-4 or IL-5. However, IL-3 levels in model A closely correlated with the rate of leukocyte clearance by apoptosis and were observed concomitant with a decline in BHR. Blockage of IL-3 in model B increased leukocyte apoptosis but reduced tissue eosinophilia and BHR. The use of mouse models in which a single different administration of allergen is associated with a failure/success to resolve inflammation and BHR by 72 h postchallenge indicates a link between IL-3 production, leukocyte apoptosis, and BHR responses.
Subject(s)
Apoptosis/immunology , Bronchial Hyperreactivity/immunology , Bronchial Hyperreactivity/pathology , Interleukin-3/physiology , Leukocytes/pathology , Lung/immunology , Lung/pathology , Animals , Apoptosis/genetics , Bronchial Hyperreactivity/genetics , Cell Movement/genetics , Cell Movement/immunology , Cell Survival/genetics , Cell Survival/immunology , Cytokines/biosynthesis , Disease Models, Animal , Eosinophilia/genetics , Eosinophilia/immunology , Eosinophilia/pathology , Female , Immunoglobulin E/biosynthesis , Immunoglobulin E/deficiency , Immunoglobulin E/genetics , Inflammation/genetics , Inflammation/immunology , Inflammation/pathology , Interleukin-3/immunology , Kinetics , Leukocytes/immunology , Leukocytes/metabolism , Mice , Mice, Inbred BALB C , Mice, Knockout , Respiratory Hypersensitivity/genetics , Respiratory Hypersensitivity/immunology , Respiratory Hypersensitivity/pathology , T-Lymphocytes/immunology , T-Lymphocytes/metabolismABSTRACT
Optimal T cell activation requires engagement of CD28 with its counterligands B7-1 and B7-2. Inducible costimulator (ICOS) is the third member of the CD28/CTLA4 family that binds a B7-like protein, B7RP-1. Administration of ICOS-Ig attenuates T cell expansion following superantigen (SAg) administration, but fails to regulate either peripheral deletion or anergy induction. ICOS-Ig, but not CTLA4-Ig, uniquely regulates SAg-induced TNF-alpha production, whereas IL-2 secretion is modulated by CTLA4-Ig, but not ICOS-Ig. In contrast, both ICOS and CD28 are required for complete attenuation of IL-4 production. Our data suggest that ICOS and CD28 regulate T cell expansion and that ligation of either CD28 or ICOS can either uniquely regulate cytokine production (IL-2/TNF-alpha) or synergize for optimal cytokine production (IL-4) after SAg administration.
Subject(s)
Adjuvants, Immunologic/physiology , Antigens, Differentiation, T-Lymphocyte/physiology , CD28 Antigens/physiology , Immunoconjugates , Signal Transduction/immunology , Abatacept , Animals , Antigens, Bacterial/administration & dosage , Antigens, Bacterial/pharmacology , Antigens, CD , Antigens, Differentiation/pharmacology , CTLA-4 Antigen , Cells, Cultured , Clonal Anergy/immunology , Clonal Deletion/immunology , Cytokines/antagonists & inhibitors , Cytokines/biosynthesis , Enterotoxins/administration & dosage , Enterotoxins/pharmacology , Inducible T-Cell Co-Stimulator Protein , Injections, Intravenous , Lymphocyte Activation/immunology , Mice , Mice, Inbred BALB C , Receptors, Antigen, T-Cell, alpha-beta/biosynthesis , Staphylococcus aureus/immunology , T-Lymphocytes/immunology , T-Lymphocytes/metabolismABSTRACT
T cells are critical mediators of inflammation and as such, their migration to inflammatory sites is a tightly controlled process involving a complex series of molecules expressed by a variety of cell types. As our appreciation of the mechanisms governing T cell surveillance, activation, differentiation, and subsequent homing to sites of inflammation has advanced, the opportunity to develop novel therapeutic agents that modulate the immune system has increased. Importantly, the possibility of specifically targetting subpopulations of effector cells raises the exciting potential for the development of novel agents that selectively modify the immune response to allergens, without resulting in generalized immune suppression.
Subject(s)
Respiratory Hypersensitivity/therapy , T-Lymphocytes/immunology , Allergens/immunology , Humans , Immune Tolerance/immunology , Respiratory Hypersensitivity/immunologyABSTRACT
The aim of this study was to investigate whether dendritic cells (DCs) can induce sensitization to aeroallergen in a mouse model of allergic asthma. Ovalbumin-pulsed (OVA-pulsed) or unpulsed myeloid DCs that were injected into the airways of naive mice migrated into the mediastinal lymph nodes. When challenged 2 weeks later with an aerosol of OVA, activated CD4 and CD8 lymphocytes, eosinophils, and neutrophils were recruited to the lungs of actively immunized mice. These CD4(+) lymphocytes produced predominantly IL-4 and IL-5 but also IFN-gamma, whereas CD8(+) lymphocytes produced predominantly IFN-gamma. Histological analysis revealed perivascular and peribronchial eosinophilic infiltrates and goblet cell hyperplasia. Studies in IL-4(-/-) and CD28(-/-) mice revealed that production of IL-4 by host cells and provision of costimulation to T cells by DCs were critical for inducing the response. Lung CD4(+) T cells strongly expressed the Th2 marker T1/ST2, and signaling through this molecule via a ligand expressed on DCs was essential for the establishment of airway eosinophilia. These data demonstrate that DCs in the airways induce sensitization to inhaled antigen and that molecules expressed on the surface of these cells are critical for the development of Th2-dependent airway eosinophilia.
Subject(s)
Antigens/administration & dosage , Asthma/etiology , Dendritic Cells/immunology , Pulmonary Eosinophilia/etiology , Th2 Cells/immunology , Administration, Inhalation , Allergens/administration & dosage , Animals , Asthma/immunology , Asthma/pathology , CD28 Antigens/genetics , CD28 Antigens/metabolism , Cytokines/metabolism , Disease Models, Animal , Interleukin-4/genetics , Interleukin-4/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Mutant Strains , Ovalbumin/administration & dosage , Ovalbumin/immunology , Pulmonary Eosinophilia/immunology , Pulmonary Eosinophilia/pathology , Signal TransductionABSTRACT
While CD28 is critical for expansion of naive T cells, recent evidence suggests that the activation of effector T cells is largely independent of CD28/B7. We suggest that ICOS, the third member of the CD28/CTLA-4 family, plays an important role in production of IL-2, IL-4, IL-5, and IFNgamma from recently activated T cells and contributes to T cell-dependent B help in vivo. Inhibition of ICOS attenuates lung mucosal inflammation induced by Th2 but not Th1 effector populations. Our data indicate a critical function for the third member of the CD28 family in T cell-dependent immune responses.