Physiological and Locomotor Profiling Enables to Differentiate Between Sprinters, 400-m Runners, and Middle-Distance Runners.

ABSTRACT: Thron, M, Woll, A, Doller, L, Quittmann, OJ, Härtel, S, Ruf, L, and Altmann, S. Physiological and locomotor profiling enables to differentiate between sprinters, 400-m runners, and middle-distance runners. J Strength Cond Res XX(X): 000-000, 2024-Different approaches exist for characterizing athletes, e.g., physiological and locomotor profiling. The aims of this study were to generate and compare physiological and locomotor profiles of male and female runners and to evaluate relationships between the different approaches. Thirty-four highly trained adolescent and young adult female and male athletes (n = 11 sprinters; n = 11,400-m runners; n = 12 middle-distance runners) performed two 100-m sprints on a running track to determine maximal sprinting speed (MSS) and maximal lactate accumulation rate (cLamax). A cardiopulmonary exercise test was performed on a treadmill to determine maximal aerobic speed (MAS) and maximal oxygen uptake (V̇o2max). Anaerobic speed reserve (ASR) was calculated as the difference between MSS and MAS. Group comparisons were conducted with a 2-way ANOVA (discipline × sex; p < 0.05) and Bonferroni post hoc tests and Cohen's d as effect size. Parameters were correlated by Pearson's correlation coefficients. Maximal aerobic speed and V̇o2max were higher in 400-m and middle-distance runners compared with sprinters (p ≤ 0.02; -2.24 ≤ d ≤ -1.29). Maximal sprinting speed and cLamax were higher in sprinters and 400-m runners compared with middle-distance runners (0.03 ≤ p ≤ 0.28; 0.73 ≤ d ≤ 1.23). Anaerobic speed reserve was highest in sprinters and lowest in middle-distance runners (p ≤ 0.03; 1.24 ≤ d ≤ 2.79). High correlations were found between ASR and MAS, MSS, and cLamax (p < 0.01; -0.55 ≤ r ≤ 0.91) and between cLamax and MSS (p < 0.01; r = 0.74). Our results indicate that athletes of different sprinting and running disciplines show differing physiological and locomotor profiles, and that the parameters of these approaches are related to each other. This can be of interest for assessing strengths and weaknesses (e.g., for talent identification) or training prescription in these disciplines.

Normative Reference Centiles for Sprint Performance in High-Level Youth Soccer Players: The Need to Consider Biological Maturity.

PURPOSE: To compute reference centiles for 5- and 30-m sprint times relative to chronological and skeletal age in youth soccer players. Subsequently, to compare individual's sprint performance scores derived from the chronological and skeletal age reference centiles. METHODS: Sprint times were collected for a sample of male U11 to U19 soccer players (n = 1745 data points). Skeletal age data were available for a subsample (n = 776 data points). Reference centiles were fitted using generalized additive models for location, scale, and shape. Individual z scores relative to chronological and skeletal age reference centiles were computed and compared for each maturity group (late, on-time, early, and very early) using standardized mean differences (SMD). RESULTS: Reference centiles for chronological age increased more rapidly between 10.5 and 15.5 years, while reference centiles for skeletal age increased more rapidly between 13.0 and 16.5 years. Differences in chronological and skeletal z scores for very early (SMD: -0.73 to -0.43) and late (SMD: 0.58 to 1.29) maturing players were small to large, while differences for early (SMD: -0.30 to -0.19) and on-time (SMD: 0.16 to 0.28) were trivial to small. CONCLUSION: Reference centiles provide a valuable tool to assist the evaluation of sprint performance in relation to chronological and skeletal age for talent identification purposes in youth soccer players.

Assessing anaerobic speed reserve: A systematic review on the validity and reliability of methods to determine maximal aerobic speed and maximal sprinting speed in running-based sports.

PURPOSE: Locomotor profiling using anaerobic speed reserve (ASR) enables insights into athletes' physiological and neuromuscular contributing factors and prescription of high-intensity training beyond maximal aerobic speed (MAS). This systematic review aimed to determine the validity and reliability of different methods to assess the characteristics of ASR, i.e., MAS and maximal sprinting speed (MSS). METHODS: A comprehensive search of the PubMed and Web of Science databases was conducted according to the PRISMA guidelines. Studies were included if they reported data on validity and/or reliability for methods to assess MAS or MSS. RESULTS: 58 studies were included with 28 studies referring to MAS and 30 studies to MSS. Regarding MAS, different methods for cardiopulmonary exercise testing yielded different values (four out of seven studies) of MAS (Cohen's d (ES) = 0.83-2.8; Pearson's r/intraclass correlation coefficient (r/ICC) = 0.46-0.85). Criterion validity of different field tests showed heterogeneous results (ES = 0-3.57; r/ICC = 0.40-0.96). Intraday and interday reliability was mostly acceptable for the investigated methods (ICC/r>0.76; CV<16.9%). Regarding MSS, radar and laser measurements (one out of one studies), timing gates (two out of two studies), and video analysis showed mostly good criterion validity (two out of two studies) (ES = 0.02-0.53; r/ICC = 0.93-0.98) and reliability (r/ICC>0.83; CV<2.43%). Criterion validity (ES = 0.02-7.11) and reliability (r/ICC = 0.14-0.97; CV = 0.7-9.77%) for global or local positioning systems (seven out of nine studies) and treadmill sprinting (one out of one studies) was not acceptable in most studies. CONCLUSION: The criterion validity of incremental field tests or shuttle runs to examine MAS cannot be confirmed. Results on time trials indicate that distances adapted to the participants' sporting background, fitness, or sex might be suitable to estimate MAS. Regarding MSS, only sprints with radar or laser measures, timing gates, or video analysis provide valid and reliable results for linear sprints of 20 to 70 m.

Effective playing time affects physical match performance in soccer: An analysis according to playing position.

This study aimed to analyse the influence of effective playing time on physical match performance according to playing position in professional soccer. Official match data from 267 matches (3,731 single observations) during the 2018/2019 season of the German Bundesliga were used and the effective playing time (duration of play after subtracting the time taken up by stoppages, substitutions, injuries, and goals) was captured for each match. The physical match performance parameters total distance, high-intensity distance, sprinting distance, maximum velocity, and accelerations were analysed. Players were categorized as central defender, wide defender, central defensive midfielder, central offensive midfielder, wide midfielder, and forward. Effective playing time influenced physical match performance, with total distance and accelerations (r = 0.48-0.61) being the most and high-intensity distance, sprinting distance, and maximum velocity (r = -0.17-0.03) the least affected parameters. Players covered on average 10% more total distance and performed 13% more accelerations, while sprinting 7-10% less in matches with long (> 65 min) compared to short (< 50 min) effective playing times. The influence of effective playing time was rather similar between playing positions. Still, physical performance of wide midfielders and forwards partly deviated from the pattern observed in the other positions. Coaches and practitioners should be aware that effective playing time influences physical match performance in the German Bundesliga, while special attention should be given to wide midfielders and forwards. Effective playing time and its general and position-specific effects should be taken into account when interpreting physical match performance, thereby facilitating load management practices and training design.

Sprint and jump performances of female athletes are enhanced in the evening but not associated with individual chronotype.

Sprint and jump performances represent performance-determining parameters in individual and team sports. Fluctuations in performance depending on the daytime raise the question of the best time to train and compete. Given the scarce research on females, this study aimed to analyze the influence of daytime on sprint and jump performances and to investigate whether the performance difference is related to the chronotype. Thus, 23 female sports students completed a questionnaire to assess their chronotype followed by performing two 30 m sprints, 5 Repeated Jump Tests (5RJT), and countermovement jumps (CMJ) in the morning (7:00-9:00 h) and evening (17:00-19:00 h). Time after 5 m, 10 m, and 30 m during the sprints, reactive strength index (RSI) during the 5RJTs, and jump height during the CMJs were examined. The performance during the 30 m sprint (t(22) = 5.28, p < 0.01 moderate effect size: 0.50) and the two jump tests (5RJT: t(22) = 8.27, p < 0.01 large effect size: 0.95; CMJ: t(22) = 5.46, p < 0.01 moderate effect size: 0.79) were significantly better in the evening than in the morning. There was no significant correlation between chronotype and the time-of-day effect. The results should be considered when planning training and competition.

Air pollution, a worthy opponent? How pollution levels impair athlete performance across physical, technical, and cognitive domains.

INTRODUCTION: Air pollution is a global issue known to effect on human health and performance. In the context of highly skilled athletes, the influence of air pollution on players' physical and technical abilities are established, yet its effects on cognitive performance have received little consideration. This study aims to address this research gap by comprehensively examining the influence of air pollution on the performance of highly skilled athletes using a holistic approach, including both the athlete's brain and body. METHODS: Between 2016 and 2022, a total of 799 soccer players (578 males, 221 females) belonging to a German professional first division club were measured on a battery of performance assessments, including physical, technical, and cognitive tests. The performance data were combined with the average daily concentration of three pollutants: PM10, O3 and NO2. RESULTS: Increased levels of PM10 and O3 were primarily associated with decreased physical and technical performance, including slower sprinting times, impaired change of direction and worse speed and accuracy in the technical assessment. For instance, if the assessment test was held when PM10 levels were at 20 µg/m3, players ran an average 22 ms slower on the 30 m sprint test, 36 ms slower on the change of direction test and showed a 1 % decrease in accuracy on the technical assessment (p < .001). Furthermore, higher concentrations of NO2 negatively impacted cognitive performance across four separate tests of athletes' executive functions (p < .05). CONCLUSION: By encompassing physical, technical, and cognitive assessments, this study highlights the multifaceted nature of performance impairments resulting from air pollution exposure in a population characterized by have exceptional abilities across all three domains. These findings underscore the widespread impact of pollution on a diverse sample of athletes and emphasize the need to consider air pollution in the broader context of its effects on human health and the environment.

Air pollution and elite adolescent soccer players' performance and well-being; an observational study.

INTRODUCTION: Exercising outdoors may inadvertently lead to individuals inhaling levels of air pollution that may be detrimental to their health and activity-related performance. Endurance athletes are a particularly susceptible subgroup due to their high ventilation rates sustained over prolonged periods of time coupled with high training loads that often occur outdoors. In this study, we estimate the effects of air pollution on a series of athletic performance parameters in an elite adolescent soccer team. METHODS: External, internal, and subjective loads and wellness questionnaires were recorded for the 26 matches and 197 training sessions carried out during the 2018-19 season for a U19 team competing in Germany. Each session was combined with hourly information on the concentration of PM10, O3 and NO2 in spatial proximity to each playing field for the duration of training or playing. RESULTS: Increases in PM10 and O3 had significant (p <.001) associations with decreasing total distance (m) ran per session. Furthermore, increases in O3 and NO2 concentrations were related to an increase in average heart rate (p <.05). Moreover, increases in PM10 concentration was associated with increased rating of perceived exertion (p <.001). Last, the total inhaled dose of O3 and NO2 over one session was linked to significant (p <.05) decreases in athletes' wellness scores on the following morning. DISCUSSION: We find supporting evidence of the negative effects of air pollution in elite adolescent soccer players in both matches and training. The negative impacts observed on several aspects of performance are present within an elite team that regularly trained in pollution levels well within the normal ranges of what the World Health Organisation (WHO) reports to be suitable air quality. Therefore, mitigation strategies such as monitoring the air quality at the training pitch are recommended to reduce athlete exposure to air pollution even when exercising in moderate air quality.

Assessing the usefulness of submaximal exercise heart rates for monitoring cardiorespiratory fitness changes in elite youth soccer players.

AIM: This study aimed to assess the value of monitoring fitness in elite youth soccer players (U15 to U19 age groups) by analyzing the concomitant changes in heart rate at submaximal intensity (HR12km/h) and the velocity at a lactate concentration of 4 mmol/l (v4mmol/l). METHODS: Players were tested by means of an incremental treadmill test on two occasions during the summer pre-season in two consecutive seasons. Based on data from a total of 170 test comparisons from the U15 (n = 48 test comparisons), U16 (n = 40 test comparisons), U17 (n = 46 test comparisons), and U19 (n = 36 test comparisons) age groups, the agreement between substantial changes in HR12km/h and v4mmol/l was analyzed using the threshold combination of HR12km/h = 4.5% and v4mmol/l = 6.0%. RESULTS: Results revealed 2% full mismatches, 36% partial agreements, and 62% full agreements for the whole sample in terms of fitness change interpretation between both variables. The respective values for the U15 to U19 age groups ranged between 0% and 5% full mismatches, 28-44% partial agreements, and 56-68% full agreements with no meaningful differences between age groups. CONCLUSION: In conclusion, our findings confirm the practical value of using HR12km/h to monitor fitness changes in elite youth soccer players when lactate sampling during incremental tests is not possible.

Soccer coaches vs. sport science and medicine staff: who can more accurately predict the skeletal age of high-level youth soccer players?

Biological maturity is an important aspect in the context of talent identification and development processes within elite youth soccer players. The aim of this study was to investigate the accuracy of soccer coaches (SC) as well as sports science and medicine staff (SSMS) to predict the skeletal age of high-level youth soccer players. We also aimed to evaluate the inter-rater reliability of the skeletal age predictions among the SC and SSMS. Skeletal ages were collected for 89 male academy soccer players registered for the U12 to U16 age groups at a professional German Bundesliga club. In addition, 12 SC and five SSMS provided their skeletal age predictions for each player of their respective age group. Standardised mean differences and equivalence testing were performed between actual and predicted skeletal ages. Intra-class correlations (ICC) were calculated to assess the inter-rater reliability. For the SC, differences between predicted and actual skeletal ages were trivial and equivalent to zero for the U12, U14, and entire sample, while for the SSMS, standardised mean differences ranged from trivial to small for all age groups and the entire sample. ICC for skeletal age predictions for the entire sample was good among the SC and excellent among the SSMS, but was somewhat lower when age groups were analysed separately. While, on average, predictions were close to the actual skeletal age, SC were slightly more accurate than the SSMS. However, variability among the SSMS was large on an individual level.

Psychophysiological Responses to a Preseason Training Camp in High-Level Youth Soccer Players.

PURPOSE: This study aimed to examine the responsiveness of commonly used measurement instruments to a short training camp by examining the time course of psychophysiological responses in high-level youth soccer players. METHODS: Monitoring was carried out in 14 U15 male soccer players of 1 professional youth academy. Players provided data 3 days prior to (D - 3), during (D2-D4), and 1 (D + 1) and 4 days (D + 4) after the camp: 4 items for the Short Recovery and Stress Scale (SRSS), a countermovement jump (CMJ), and a submaximal run to assess exercise heart rate and heart-rate recovery. Training load during the camp followed an alternating low-high pattern, with lower training loads on D1 and D3 and higher training loads on D2 and D4. RESULTS: Changes in SRSS physical performance capability, emotional balance, overall recovery, muscular stress, and overall stress were small to moderate on D3 and moderate to large on D + 1, while changes were trivial on D + 4. Some CMJ parameters related to the eccentric phase were slightly improved on D3, and these parameters were slightly impaired on D4. Changes in CMJ parameters were trivial on D + 1 and D + 4. After a moderate decrease in exercise heart rate on D3, there was a small decrease on D + 4 and a moderate increase in heart-rate recovery. CONCLUSION: Measurement instruments such as the SRSS and submaximal runs can be used to monitor acute psychophysiological responses to load, while the CMJ may provide little insight during periods of intensified training load.

Longitudinal development of physical, perceptual-cognitive and skill predictors of talent in academy and professional female football players.

Existing literature on talent development predominantly focuses on male athletes, with limited representation of female athletes. This study aims to address this gap by examining the long-term development of female football players in an elite club. Routine lab-based assessments were conducted on 238 athletes across six teams for 7 years to determine how physical, perceptual-cognitive, and skill performance predictors fluctuated with player age and developmental stage (sampling 9-11y, specializing 12-14y, investment 15-18y, and performance +18y). The developmental stage was a significant predictor of improved performance for each talent indicator (p < .001), with each consecutive stage significantly outperforming the previous stage in all domains with moderate to large effect sizes (0.07-0.40 ηp2). Improvement rate was higher in young adolescence (<15) and slower approaching adulthood (>18y). Playing position influenced performance scores on several physical and technical skill predictors (p ≤ .001), but not perceptual-cognitive ones (p ≥ .11). Players progressed continuously from the sampling to the performance stage, contradicting previously reported plateaus observed when athletes reached the investment stage. Benchmark data are provided across age and playing position to better understand what is required for successful participation at an elite level at varying age groups in female soccer.

Overestimation of maximal aerobic speed by the Université de Montréal track test and a 1500-m-time trial in soccer.

Introduction: Maximal aerobic speed (MAS), usually measured by cardiopulmonary exercise testing (CPET) on a treadmill, is gaining popularity in soccer to determine aerobic performance. Several field tests are used to estimate MAS, although, gold standard methods are still not clarified. Therefore, this work aims 1) to compare two different CPET based methods to assess MAS and 2) to investigate the convergent validity of two common field tests to estimate MAS in soccer. Methods: Thirteen trained male soccer players completed an CPET on a treadmill to determine two VO2-kinetic based definitions of MAS (MASPlateau = speed at onset of VO2-plateau = gold standard; MAS30s = first speed of 30-s-interval of VO2max), the Université de Montreal Track Test (UMTT; VUMTT = speed of the last stage), and a 1500-m-time trial (1500-m-TT; V1500m = average speed). MASPlateau, MAS30s, VUMTT, and V1500m were compared using ANOVA. Additionally, limits of agreement analysis (LoA), Pearson's r, and ICC were calculated between tests. Results: MAS30s, VUMTT, and V1500m significantly overestimated MASPlateau by 0.99 km/h (ES = 1.61; p < 0.01), 1.61 km/h (ES = 2.03; p < 0.01) and 1.68 km/h (ES = 1.77; p < 0.01), respectively, with large LoA (-0.21 ≤ LoA≤3.55), however with large-to-very large correlations (0.65 ≤ r ≤ 0.87; p ≤ 0.02; 0.51 ≤ ICC≤ 0.85; p ≤ 0.03). Discussion: The overestimation and large LoA of MASPlateau by all estimates indicate that 1) a uniform definition of MAS is needed and 2) the UMTT and a 1500-m-TT seem questionable for estimating MAS for trained soccer players on an individual basis, while regression equations might be suitable on a team level. The results of the present work contribute to the clarification of acquisition of MAS in soccer.

Differences in Physical Match Performance and Injury Occurrence Before and After the COVID-19 Break in Professional European Soccer Leagues: A Systematic Review.

BACKGROUND: Due to the COVID-19 pandemic, matches and soccer-specific training were suspended for several weeks, matches after resumption were congested, and substitutions per team and game increased from three to five. OBJECTIVE: The aim of this review was to examine possible differences in physical match performance and injuries between before and after the COVID-19 induced break of matches and training in professional male European soccer leagues during the 2019/2020 season. METHODS: A systematic search identified all scientifically peer-reviewed publications involving elite male soccer players competing in the European leagues which reported physical match performance variables such as total running distance and running distance at different speed zones and/or injury parameters pre- and post-COVID-19 induced break. RESULTS: In total, 11 articles were included, which were coming from German Bundesliga, Polish Ekstraklasa, Croatian HNL, Spanish La Liga, and Italian Serie A. In all studies investigating the German Bundesliga, most parameters of physical match performance remained unaffected (0.08 ≤ p ≤ 0.82; - 0.15 ≤ ES 0.15), while studies investigating the Polish Ekstraklasa (p ≤ 0.03; - 0.27 ≤ ES - 0.18), Croatian HNL (p ≤ 0.04; - 1.42 ≤ ES ≤ 1.44), Spanish La Liga (p ≤ 0.017; - 0.32 ≤ ES ≤ 5.5), and Italian Serie A (p ≤ 0.014; - 1.01 ≤ ES 0.24) showed a decrease in most parameters of physical match performance after the COVID-19 break. Injury rates were only investigated by studies targeting the German Bundesliga and Italian Serie A. In the majority of studies (3 out of 4 studies), there occurred no difference in injuries between pre- and post-COVID-19 break (p > 0.05; ES = N/A). CONCLUSION: Results indicate that Bundesliga teams maintained physical match performance during the 9-weeks break in matches and 3-weeks break in group training, whereas a longer match and group training interruption up to 15 weeks and 8 weeks, respectively, in the other leagues appeared to lead to a decreased physical match performance. Regarding injuries, we speculate that the increase in substitutions from 3 to 5 substitutions per game might prevent an increase in injury occurrence during matches. The underlying studies' results provide hints for possible upcoming unexpected interruptions with respect to optimal physical preparations for the resumption of matches and a congested schedule to maintain physical match performance, or for possible rule changes such as 5 instead of 3 substitutions to avoid physical overload during congested match schedules.

Injury incidence, severity, and burden in elite youth soccer players - A 3-year prospective study.

OBJECTIVES: This study aimed to investigate age-related injury incidence, severity, and burden over a 3-year period in U14 to U19 elite male soccer players in a German youth academy. DESIGN: Descriptive epidemiology study. METHODS: Time-loss injuries of 166 unique players were prospectively documented and injury incidence, severity, and burden were calculated for each age group, body part, and type of injuries. RESULTS: A total of 562 time-loss injuries were recorded. Across all age groups (U14 to U19), injury incidence was 42.2 per squad season (ranging from 30.5 in the U19 to 67.7 in the U15), severity was 11.0 days lost (ranging from 8.0 in the U15 to 17.0 in the U17), and burden was 464.1 days lost per squad season (ranging from 304.9 in the U19 to 756.7 in the U14). The thigh, knee and ankle were the body parts with the highest injury incidence (9.2; 5.9; 5.9), severity (11.0; 16.0; 13.0), and burden (101.6; 94.9; 76.1). Cruciate ligament injuries had both the highest burden (164.4) and severity (137.0), and muscle injuries the highest incidence (11.0) of all types of injuries. U15 had the highest injury incidence (67.7) of all age groups. U14 and U17 had both the highest injury severity (15.0; 17.0) and burden (756.7; 618.8) of all age groups. Age-related patterns were observed for selected types of injuries such as muscle injuries, tendinopathy, bone-stress injury, and fractures. CONCLUSIONS: Injury incidence, severity, and burden substantially differed descriptively between age groups and clear age-related patterns for selected types of injuries were evident.

Does Technical Match Performance in Professional Soccer Depend on the Positional Role or the Individuality of the Player?

The aim of the study was to examine the impact of the positional role and the individuality on the technical match performance in professional soccer players. From official match data of the Bundesliga season 2018/19, technical performance [short (<10 m)/medium (10-30 m)/long (>30 m) passes, dribblings, ball possessions] of all players who played during the season were analyzed (normative data). Five playing positions (center back, full back, central midfielder, wide midfielder and forward) were distinguished. As the contextual factor tactical formation is known to influence match performance, this parameter was controlled for. Further, those players who played at minimum four games in at least two different playing positions were included in the study sample (n = 13). The technical match performance of the players was analyzed in relation to the normative data regarding the extent to which the players either adapted or maintained their performance when changing the playing position. When switching playing positions, positional role could explain 3-6% of the variance in short passes and ball possessions and 27-44% of the variance in dribblings, medium passes, and long passes. Moreover, we observed large interindividual differences in the extent to which a player changed, adapted, or maintained his performance. In detail, five players clearly adapted their technical performance when changing playing positions, while five players maintained their performance. Coaches can use these findings to better understand the technical match performance of single players and further, to estimate the impact of a change in the positional role on the technical performance of the respective player.

Match-related physical performance in professional soccer: Position or player specific?

The purpose of this study was to examine to what extent the physical match performance of professional soccer players is both position and player specific. First, official match data from the 2019/20 German Bundesliga season was used to search for players that met the inclusion criteria of playing a minimum of four entire matches in at least two different playing positions. Overall, 25 players met the criteria prior to the COVID-19 induced break, playing a minimum of eight matches. Second, the physical match performance of these players was analyzed separately for each position they played. The following four parameters were captured: total distance, high-intensity distance, sprinting distance, and accelerations. Third, the 25 players' physical match performance data was then compared to normative data for each position they played to understand whether players adapted their physical performance (position dependent), or maintained their performance regardless of which position they were assigned to (position independent). When switching the position, the change in physical match performance of the respective players could be explained by 44-58% through the normative positional data. Moreover, there existed large individual differences in the way players adapted or maintained their performance when acting in different positions. Coaches and practitioners should be aware that some professional soccer players will likely incur differences in the composition of physical match performance when switching positions and therefore should pay special consideration for such differences in the training and recovery process of these players.

Sex-Specific Relationship between the Cardiorespiratory Fitness and Plasma Metabolite Patterns in Healthy Humans-Results of the KarMeN Study.

Cardiorespiratory fitness (CRF) represents a strong predictor of all-cause mortality and is strongly influenced by regular physical activity (PA). However, the biological mechanisms involved in the body's adaptation to PA remain to be fully elucidated. The aim of this study was to systematically examine the relationship between CRF and plasma metabolite patterns in 252 healthy adults from the cross-sectional Karlsruhe Metabolomics and Nutrition (KarMeN) study. CRF was determined by measuring the peak oxygen uptake during incremental exercise. Fasting plasma samples were analyzed by nuclear magnetic resonance spectroscopy and mass spectrometry coupled to one- or two-dimensional gas chromatography or liquid chromatography. Based on this multi-platform metabolomics approach, 427 plasma analytes were detected. Bi- and multivariate association analyses, adjusted for age and menopausal status, showed that CRF was linked to specific sets of metabolites primarily indicative of lipid metabolism. However, CRF-related metabolite patterns largely differed between sexes. While several phosphatidylcholines were linked to CRF in females, single lyso-phosphatidylcholines and sphingomyelins were associated with CRF in males. When controlling for further assessed clinical and phenotypical parameters, sex-specific CRF tended to be correlated with a smaller number of metabolites linked to lipid, amino acid, or xenobiotics-related metabolism. Interestingly, sex-specific CRF explanation models could be improved when including selected plasma analytes in addition to clinical and phenotypical variables. In summary, this study revealed sex-related differences in CRF-associated plasma metabolite patterns and proved known associations between CRF and risk factors for cardiometabolic diseases such as fat mass, visceral adipose tissue mass, or blood triglycerides in metabolically healthy individuals. Our findings indicate that covariates like sex and, especially, body composition have to be considered when studying blood metabolic markers related to CRF.

Construct validity of age at predicted adult height and BAUS skeletal age to assess biological maturity in academy soccer.

BACKGROUND: The assessment of biological maturity status plays an important role in talent identification and development programs. AIM: To compare age at predicted adult height and BAUS skeletal age as indicators of biological maturity status in youth soccer players using a construct-validity approach. SUBJECTS AND METHODS: Participants were 114 players from the U12 to U17 age groups of a professional youth soccer academy. Maturity status was determined via percentage of predicted adult height based upon the Khamis-Roche method (somatic maturity) and assessed via the SonicBone BAUSTM system (skeletal maturity). Convergent and known-groups validity were evaluated between maturity assessment methods and by comparing maturity-related selection biases across age groups. RESULTS: Although maturity status indicators were largely interrelated (r = .94, 95%CL 0.91-0.96), concordance (κ = 0.31 to 0.39) and Spearman's rank-order correlations (ρ = 0.45-0.52) of classification methods were moderate. A selection bias towards early maturing players emerged in the U14 age group which remained relatively consistent through to the U17 age group. CONCLUSIONS: Results confirm the construct-validity of both methods to assess biological maturity status although further validation relative to established indicators of biological maturity is needed. Furthermore, caution is also warranted when interpreting maturity status classification methods interchangeably given the poor concordance between classification methods.

Agility testing in amateur soccer: A pilot study of selected physical and perceptual-cognitive contributions.

The purpose of this study was to examine the relationships of physical and perceptual-cognitive factors with agility performance in amateur soccer players. Fifteen male amateur soccer players (age, 24.5 ± 1.9 years) completed a linear-sprint test with splits at 5 m, 10 m, and 30 m, a change-of-direction test of 12 m with 2 pre-planned directional changes of 45° at 2 m and 7 m, and a soccer-specific agility test with same movement pattern as the change-of-direction test but with the inclusion of a human stimulus performing passing movements. Additionally, the perceptual-cognitive deficit (agility performance minus change-of-direction performance) was calculated. In relation to agility performance, linear-sprint performance showed large relationships, which were higher with increasing sprint distance (5 m, r = 0.57; 10 m, r = 0.59; 30 m, r = 0.69), change-of-direction performance a very large relationship (r = 0.77), and the perceptual-cognitive deficit a large relationship (r = 0.55). The findings of this study highlight the relatively high contribution of both physical (i.e., linear-sprint and change-of-direction performance) and perceptual-cognitive factors (i.e., perceptual-cognitive deficit) in relation to soccer-specific agility performance at an amateur level. Consequently, such elements might be recommended to be included in training programs aimed at improving agility performance at this playing level. Moreover, the here introduced perceptual-cognitive deficit allows for a convenient and likewise thorough analysis of agility performance. Future studies should investigate the effects of both physically and perceptual-cognitive oriented training interventions on agility performance, which is considered a key element for success in soccer.

Using Submaximal Exercise Heart Rate for Monitoring Cardiorespiratory Fitness Changes in Professional Soccer Players: A Replication Study.

PURPOSE: To assess the value of monitoring changes in fitness in professional soccer players, using changes in heart rate at submaximal intensity (HR12km/h) over the velocity at a lactate concentration of 4 mmol/L (v4mmol/L). The authors reexamined (1) a range of threshold magnitudes, which may improve detecting substantial individual changes and (2) the agreement between changes in these 2 variables. METHODS: On at least 2 occasions during different moments of the season, 97 professional soccer players from Germany (first, second, and fourth division) completed an incremental test to determine HR12km/h and v4mmol/L. Optimal thresholds for changes in HR12km/h and v4mmol/L were assessed, using various methods (eg, smallest worthwhile change + typical error [TE], successive reiterations approach). Agreement between both variable changes was examined for the whole sample (225 comparisons), 4 different subgroups (depending on the moment of the season), and in an individual over 6 years (n = 23 tests). RESULTS: Changes of 4.5% and 6.0% for HR12km/h and v4mmol/L, respectively, were rated as optimal to indicate substantial changes in fitness. Depending on the (sub)groups analyzed, these thresholds yielded 0% to 2% full mismatches, 22% to 38% partial agreements, and 60% to 78% full agreements in terms of fitness change interpretation between both variables. CONCLUSIONS: When lactate sampling during incremental tests is not possible, practitioners willing to monitor adult professional soccer players' (Germany; first, second, and fourth division) training status can confidently implement short, 3-minute submaximal runs, with 4.5% changes in HR12km/h being indicative of true substantial fitness changes, with 60% to 78% accuracy. Future studies should investigate the potential role of confounding factors of HR12km/h to improve changes in fitness prediction.