Using single and double laser pulses on the molecular Ni4@C48H36 system to design integrated nanospintronic units.

The accomplishment of long-distance spin transfer scenarios between several magnetic centers is a big challenge for building and supporting spin-logic units for developing future all-optical magnetic unit operations. Using high-level quantum chemistry theory CCSD and EOM-CCSD, we systematically study the ultrafast laser-induced spin-dynamics process on a carbon-based material, to which four magnetic centers are attached. We show that the CCSD method with the 6-31G basis set calculation is sensitive to the C-Ni bond length. The spin density distribution, which is computed using EOM-CCSD with LanL2DZ+ECP calculations, Mulliken population analysis, including spin-orbit-coupling (SOC) and a magnetic field, fulfills the requirements for achieving spin dynamics processes. Different local spin-flip and spin-transfer processes are accomplished within the subpicosecond regime. The impact of the propagation direction of the laser pulse by switching their polar and the azimuthal angles in spherical coordinates on the spin dynamics processes is analyzed. Double laser pulses with time delay δt ≥ 200 × FWHM yield in a realistic magnetic field gradient selectively a lateral resolution, which corresponds to distances smaller than the CMOS scale (2 nm in 2024) while our system size is comparable to the CMOS scale. Here Λ and V processes with two quasi-degenerate intermediate levels are used. We propose a model of an integrated spin-logic processor created from an array of individual spin-logic blocks, which are realized by four magnetic centers Ni. The findings of this study demonstrate the enormous potential of using laser-induced spin dynamics as the fundamental mechanism for future molecular magnetic technology.

Missense mutation (C667F) in murine ß-dystroglycan causes embryonic lethality, myopathy and blood-brain barrier destabilization.

Dystroglycan (DG) is an extracellular matrix receptor consisting of an α- and a ß-DG subunit encoded by the DAG1 gene. The homozygous mutation (c.2006G>T, p.Cys669Phe) in ß-DG causes Muscle-Eye-Brain disease with multicystic leukodystrophy in humans. In a mouse model of this primary dystroglycanopathy, approximately two-thirds of homozygous embryos fail to develop to term. Mutant mice that are born undergo a normal postnatal development but show a late-onset myopathy with partially penetrant histopathological changes and an impaired performance on an activity wheel. Their brains and eyes are structurally normal, but the localization of mutant ß-DG is altered in the glial perivascular endfeet resulting in a perturbed protein composition of the blood-brain and blood-retina barrier. In addition, α- and ß-DG protein levels are significantly reduced in muscle and brain of mutant mice. Due to the partially penetrant developmental phenotype of the C669F-ß-DG mice, they represent a novel and highly valuable mouse model to study the molecular effects of ß-DG functional alterations both during embryogenesis and in mature muscle, brain and eye, and to gain insight into the pathogenesis of primary dystroglycanopathies.

Unlocking Ultrafast Spin Transfer in Single-Magnetic-Center-Decorated Triangulene Systems.

Triangulene, as a typical open-shell graphene fragment, has attracted widespread attention for nanospintronics, promising to serve as building blocks in spin-logic units. Here, using ab initio calculations, we systematically study the laser-induced ultrafast spin-dynamic processes on triangulene nanoflakes, decorated with a transition-metal atom. The results reveal a competition between the induced magnetic center and the carbon edge of the triangulene, resulting in the coexistence of dual spin-density-distribution patterns on such single-magnetic-center systems, thus opening up possibilities of complex spin-dynamic scenarios beyond the spin flip. Interestingly, no matter what direction the spin points to, it is possible to achieve reversible spin-transfer processes using the same laser pulse. Increasing the pool of elementary processes to contain not only spin-direction-dependent but also spin-direction-independent scenarios allows for more versatile spin-logic operations, including classical handling of information and quantum computing. In the present work, we suggest downscaling nanospintronic devices by integrating triangulene-based nanostructures.

Rescue of mitochondrial import failure by intercellular organellar transfer.

Mitochondria are the powerhouses of eukaryotic cells, composed mostly of nuclear-encoded proteins imported from the cytosol. Thus, problems with the import machinery will disrupt their regenerative capacity and the cell's energy supplies - particularly troublesome for energy-demanding cells of nervous tissue and muscle. Unsurprisingly then, import breakdown is implicated in disease. Here, we explore the consequences of import failure in mammalian cells; wherein, blocking the import machinery impacts mitochondrial ultra-structure and dynamics, but, surprisingly, does not affect import. Our data are consistent with a response involving intercellular mitochondrial transport via tunnelling nanotubes to import healthy mitochondria and jettison those with blocked import sites. These observations support the existence of a widespread mechanism for the rescue of mitochondrial dysfunction.

Analysis of the GFP-labelled ß-dystroglycan interactome in HEK-293 transfected cells reveals novel intracellular networks.

Dystroglycan (DG) is a cell adhesion complex that is widely expressed in tissues. It is composed by two subunits, α-DG, a highly glycosylated protein that interacts with several extracellular matrix proteins, and transmembrane ß-DG whose, cytodomain binds to the actin cytoskeleton. Glycosylation of α-DG is crucial for functioning as a receptor for its multiple extracellular binding partners. Perturbation of α-DG glycosylation is the central event in the pathogenesis of severe pathologies such as muscular dystrophy and cancer. ß-DG acts as a scaffold for several cytoskeletal and nuclear proteins and very little is known about the fine regulation of some of these intracellular interactions and how they are perturbed in diseases. To start filling this gap by identifying uncharacterized intracellular networks preferentially associated with ß-DG, HEK-293 cells were transiently transfected with a plasmid carrying the ß-DG subunit with GFP fused at its C-terminus. With this strategy, we aimed at forcing ß-DG to occupy multiple intracellular locations instead of sitting tightly at its canonical plasma membrane milieu, where it is commonly found in association with α-DG. Immunoprecipitation by anti-GFP antibodies followed by shotgun proteomic analysis led to the identification of an interactome formed by 313 exclusive protein matches for ß-DG binding. A series of already known ß-DG interactors have been found, including ezrin and emerin, whilst significant new matches, which include potential novel ß-DG interactors and their related networks, were identified in diverse subcellular compartments, such as cytoskeleton, endoplasmic reticulum/Golgi, mitochondria, nuclear membrane and the nucleus itself. Of particular interest amongst the novel identified matches, Lamina-Associated Polypeptide-1B (LAP1B), an inner nuclear membrane protein, whose mutations are known to cause nuclear envelopathies characterized by muscular dystrophy, was found to interact with ß-DG in HEK-293 cells. This evidence was confirmed by immunoprecipitation, Western blotting and immunofluorescence experiments. We also found by immunofluorescence experiments that LAP1B looses its nuclear envelope localization in C2C12 DG-knock-out cells, suggesting that LAP1B requires ß-DG for a proper nuclear localization. These results expand the role of ß-DG as a nuclear scaffolding protein and provide novel evidence of a possible link between dystroglycanopathies and nuclear envelopathies displaying with muscular dystrophy.

Evaluation of Swin Transformer and knowledge transfer for denoising of super-resolution structured illumination microscopy data.

BACKGROUND: Convolutional neural network (CNN)-based methods have shown excellent performance in denoising and reconstruction of super-resolved structured illumination microscopy (SR-SIM) data. Therefore, CNN-based architectures have been the focus of existing studies. However, Swin Transformer, an alternative and recently proposed deep learning-based image restoration architecture, has not been fully investigated for denoising SR-SIM images. Furthermore, it has not been fully explored how well transfer learning strategies work for denoising SR-SIM images with different noise characteristics and recorded cell structures for these different types of deep learning-based methods. Currently, the scarcity of publicly available SR-SIM datasets limits the exploration of the performance and generalization capabilities of deep learning methods. RESULTS: In this work, we present SwinT-fairSIM, a novel method based on the Swin Transformer for restoring SR-SIM images with a low signal-to-noise ratio. The experimental results show that SwinT-fairSIM outperforms previous CNN-based denoising methods. Furthermore, as a second contribution, two types of transfer learning-namely, direct transfer and fine-tuning-were benchmarked in combination with SwinT-fairSIM and CNN-based methods for denoising SR-SIM data. Direct transfer did not prove to be a viable strategy, but fine-tuning produced results comparable to conventional training from scratch while saving computational time and potentially reducing the amount of training data required. As a third contribution, we publish four datasets of raw SIM images and already reconstructed SR-SIM images. These datasets cover two different types of cell structures, tubulin filaments and vesicle structures. Different noise levels are available for the tubulin filaments. CONCLUSION: The SwinT-fairSIM method is well suited for denoising SR-SIM images. By fine-tuning, already trained models can be easily adapted to different noise characteristics and cell structures. Furthermore, the provided datasets are structured in a way that the research community can readily use them for research on denoising, super-resolution, and transfer learning strategies.

Laser-induced ultrafast spin-transfer processes in non-linear zigzag carbon chain systems.

We combine the high-level quantum chemistry theory CCSD and EOM-CCSD together with local and global Λ processes to investigate the details of the laser-induced ultrafast spin manipulation scenarios in non-linear zigzag carbon chain systems Ni2@C32H32 and Ni2@C36H36. The spin density distribution, which is calculated on each many-body state using a Mulliken population analysis, fulfills the requirements to accomplish the spin dynamics processes. Various spin-flip and spin-transfer scenarios are accomplished. All the spin-dynamics processes can be achieved within subpicosecond times. Under the influence of a magnetic field, we find that the spin-transfer scenarios are preserved, while the local spin-flip scenario on a Ni atom can be significantly inhibited depending on the strength of the magnetic field. The impact of the propagation direction of the laser pulse on the spin dynamics processes by varying their polar and azimuthal angles in spherical coordinates is investigated. Additionally, we find that double laser pulses successfully induce the spin-transfer processes. Our outcomes underline the significant potential of carbon chain systems as building blocks for developing future all-optical integrated logic processing units.

Optically Driven Both Classical and Quantum Unary, Binary, and Ternary Logic Gates on Co-Decorated Graphene Nanoflakes.

Nanospintronics holds great potential for providing high-speed, low-power, and high-density logic and memory elements in future computational devices. Here, using ab initio many-body theory, we suggest a nanoscale framework for building quantum computation elements, based on individual magnetic atoms deposited on graphene nanoflakes. We show the great possibilities of this proposal by exemplarily presenting four quantum gates, namely, the unary Pauli-X, Pauli-Y, Pauli-Z, and Hadamard gates, as well as the universal classical ternary Toffoli gate, which preserves information entropy and is therefore fully reversible and minimally energy consuming. All our gates operate within the subpicosecond time scale and reach fidelities well above 90%. We demonstrate the ability to control the spin direction and localization, as well as to create superposition states and to control the quantum phase of states, which are indispensable ingredients of quantum computers. Additionally, being optically driven, their predicted operating speed by far beats that of modern quantum computers.

Ultrafast control of laser-induced spin-dynamics scenarios on two-dimensional Ni3@C63H54 magnetic system.

The concept of building logically functional networks employing spintronics or magnetic heterostructures is becoming more and more popular today. Incorporating logical segments into a circuit needs physical bonds between the magnetic molecules or clusters involved. In this framework, we systematically study ultrafast laser-induced spin-manipulation scenarios on a closed system of three carbon chains to which three Ni atoms are attached. After the inclusion of spin-orbit coupling and an external magnetic field, different ultrafast spin dynamics scenarios involving spin-flip and long-distance spin-transfer processes are achieved by various appropriately well-tailored time-resolved laser pulses within subpicosecond timescales. We additionally study the various effects of an external magnetic field on spin-flip and spin-transfer processes. Moreover, we obtain spin-dynamics processes induced by a double laser pulse, rather than a single one. We suggest enhancing the spatial addressability of spin-flip and spin-transfer processes. The findings presented in this article will improve our knowledge of the magnetic properties of carbon-based magnetic molecular structures. They also support the relevant experimental realization of spin dynamics and their potential applications in future molecular spintronics devices.

Fluorescence Microscopy in Adeno-Associated Virus Research.

Research on adeno-associated virus (AAV) and its recombinant vectors as well as on fluorescence microscopy imaging is rapidly progressing driven by clinical applications and new technologies, respectively. The topics converge, since high and super-resolution microscopes facilitate the study of spatial and temporal aspects of cellular virus biology. Labeling methods also evolve and diversify. We review these interdisciplinary developments and provide information on the technologies used and the biological knowledge gained. The emphasis lies on the visualization of AAV proteins by chemical fluorophores, protein fusions and antibodies as well as on methods for the detection of adeno-associated viral DNA. We add a short overview of fluorescent microscope techniques and their advantages and challenges in detecting AAV.

Cost-effective high-speed, three-dimensional live-cell imaging of HIV-1 transfer at the T cell virological synapse.

The availability of cost-effective, highly portable, and easy to use high-resolution live-cell imaging systems could present a significant technological break-through in challenging environments, such as high-level biosafety laboratories or sites where new viral outbreaks are suspected. We describe and demonstrate a cost-effective high-speed fluorescence microscope enabling the live tracking of virus particles across virological synapses that form between infected and uninfected T cells. The dynamics of HIV-1 proteins studied at the cellular level and the formation of virological synapses in living T cells reveals mechanisms by which cell-cell interactions facilitate infection between immune cells. Dual-color 3D fluorescence deconvolution microscopy of HIV-1 particles at frames rates of 100 frames per second allows us to follow the transfer of HIV-1 particles across the T cell virological synapse between living T cells. We also confirm the successful transfer of virus by imaging T cell samples fixed at specific time points during cell-cell virus transfer by super-resolution structured illumination microscopy.

A theoretical study of laser-induced ultrafast spin dynamics in trigonal monopyramidal iron and nickel complexes.

We present a first-principles study of the geometries, electronic structures, and laser-induced ultrafast spin dynamics in four trigonal monopyramidal complexes [tpat-BuFe]-, [tcmat-BuFe]-, [tpat-BuNi]-, and [tcmat-BuNi]- [tpa: tris-(pyrrolylmethyl)amine; tcma: tris(carbamoyl-methyl)amine; t-Bu: tert-butyl]. It is found that the low-lying level distribution of the four structures is similar, however, their spin and charge localization differs substantially. Detailed analysis demonstrates that the iron complexes have much more singly spin localized states located in the low energy region, while the nickel complexes have more charge-transfer (CT) states and more states with spin equally distributed between the Ni and the ligands. Affected by these features, more ultrafast spin-crossover (SCO) scenarios are achieved in the two iron complexes, and better CT dynamics is obtained in nickel complexes. In particular, for the CT scenarios combined with spin bifurcation, the charge is transferred from the tpa/tcma ligand to the Fe/Ni atoms, while spin-density transfer occurs in the opposite direction. Among the scenarios illustrated in the paper, the SCO processes turn out to be more complicated since they involve many more intermediate states and exhibit relatively low fidelity. In addition, the transferability of each scenario is analyzed from the absorption spectra of the initial and final states. All these results can provide significant insights into the electronic and magnetic natures of the four complexes, guide the experimental realization of the relevant scenarios, and thus promote their applications in molecular spintronics.

Experimental and Theoretical Study of the Ultrafast Dynamics of a Ni2 Dy2 -Compound in DMF After UV/Vis Photoexcitation.

Invited for this month's cover picture are the groups of Wolfgang Hübner (TU Kaiserslautern, Germany), Annie Powell (Karlsruhe Institut of Technology, Germany), and Andreas-Neil Unterreiner (Karlsruhe Institut of Technology, Germany). The cover picture shows the Dy2 Ni2 -molecular magnet being excited with a UV/Vis laser pulse, together with its time-resolved spectrum after the pulse. The comparison of the theoretical and the experimental spectra together with both the observed and the calculated relaxation times reveal, among others, three key points: the intermediate states participating in the laser-induced dynamics, the partial metal-to-oxygen charge-transfer excitations, and the order of magnitude of the coupling of the molecular magnet to the thermal bath of the environment. Read the full text of their Full Paper at 10.1002/open.202100153.

Laser-Controlled Implementation of Controlled-NOT, Hadamard, SWAP, and Pauli Gates as Well as Generation of Bell States in a 3d-4f Molecular Magnet.

Using high-level ab initio many-body theory, we theoretically propose that the Dy and the Ni atoms in the [Dy2Ni2(L)4(NO3)2(DMF)2] real molecular magnet as well as in its core, that is, the [Dy2Ni2O6] system, act as two-level qubit systems. Despite their spatial proximity we can individually control each qubit in this highly correlated real magnetic system through specially designed laser-pulse combinations. This allows us to prepare any desired two-qubit state and to build several classical and quantum logic gates, such as the two-qubit (binary) CNOT gate with three distinct laser pulses. Other quantum logic gates include the single-qubit (unary) quantum X, Y, and Z Pauli gates; the Hadamard gate (which necessitates the coherent quantum superposition of two many-body electronic states); and the SWAP gate (which plays an important role in Shor's algorithm for integer factorization). Finally, by sequentially using the achieved CNOT and Hadamard gates we are able to obtain the maximally entangled Bell states, for example, (12)(|00⟩ + |11⟩).

Dual color DMD-SIM by temperature-controlled laser wavelength matching.

Structured illumination microscopy (SIM) is a fast and gentle super-resolution fluorescence imaging technique, featuring live-cell compatible excitation light levels and high imaging speeds. To achieve SIM, spatial modulation of the fluorescence excitation light is employed. This is typically achieved by interfering coherent laser beams in the sample plane, which are often created by spatial light modulators (SLMs). Digital micromirror devices (DMDs) are a form of SLMs with certain advantages, such as high speed, low cost and wide availability, which present certain hurdles in their implementation, mainly the blazed grating effect caused by the jagged surface structure of the tilted mirrors. Recent works have studied this effect through modelling, simulations and experiments, and laid out possible implementations of multi-color SIM imaging based on DMDs. Here, we present an implementation of a dual-color DMD based SIM microscope using temperature-controlled wavelength matching. By carefully controlling the output wavelength of a diode laser by temperature, we can tune two laser wavelengths in such a way that no opto-mechanical realignment of the SIM setup is necessary when switching between both wavelengths. This reduces system complexity and increases imaging speed. With measurements on nano-bead reference samples, as well as the actin skeleton and membrane of fixed U2OS cells, we demonstrate the capabilities of the setup.

Strain manipulation of the local spin flip on Ni@B80 endohedral fullerene.

Using first principles, we theoretically investigate the strain manipulation of the ultrafast spin-flip processes on the Ni@B80 endohedral fullerene by using highly correlated quantum chemical calculations. It is shown that the ultrafast local spin flip on Ni@B80 can be achieved via Λ processes with high fidelities in both the equilibrium and distorted structures. Moreover, the applied strain on Ni@B80 can significantly lead to the redistribution of spin density, and therefore dominate the spin-flip processes. It is interesting that the strain effects on the spin-flip processes of Ni@B80 are not identical. Specifically, when a strain is applied along the direction across the Ni atom, the influence is exactly opposite to the case when the strain direction goes without crossing the Ni atom. This orientation-dependent strain effect is also demonstrated by analyzing the modulated energy gaps between the singly occupied molecular orbital (SOMO) and the lowest unoccupied molecular orbital (LUMO) of the system. The present results shed some light on the mechanical control of the magneto-optic dynamics behavior of the endohedral fullerenes, and further provide the idea that strain engineering and spin engineering can be combined for the design of nanoscale magnetic storage units and spintronic devices.

Multiscale and Multimodal Optical Imaging of the Ultrastructure of Human Liver Biopsies.

The liver as the largest organ in the human body is composed of a complex macroscopic and microscopic architecture that supports its indispensable function to maintain physiological homeostasis. Optical imaging of the human liver is particularly challenging because of the need to cover length scales across 7 orders of magnitude (from the centimeter scale to the nanometer scale) in order to fully assess the ultrastructure of the entire organ down to the subcellular scale and probe its physiological function. This task becomes even more challenging the deeper within the organ one hopes to image, because of the strong absorption and scattering of visible light by the liver. Here, we demonstrate how optical imaging methods utilizing highly specific fluorescent labels, as well as label-free optical methods can seamlessly cover this entire size range in excised, fixed human liver tissue and we exemplify this by reconstructing the biliary tree in three-dimensional space. Imaging of tissue beyond approximately 0.5 mm length requires optical clearing of the human liver. We present the successful use of optical projection tomography and light-sheet fluorescence microscopy to derive information about the liver architecture on the millimeter scale. The intermediate size range is covered using label-free structural and chemically sensitive methods, such as second harmonic generation and coherent anti-Stokes Raman scattering microscopy. Laser-scanning confocal microscopy extends the resolution to the nanoscale, allowing us to ultimately image individual liver sinusoidal endothelial cells and their fenestrations by super-resolution structured illumination microscopy. This allowed us to visualize the human hepatobiliary system in 3D down to the cellular level, which indicates that reticular biliary networks communicate with portal bile ducts via single or a few ductuli. Non-linear optical microscopy enabled us to identify fibrotic regions extending from the portal field to the parenchyma, along with microvesicular steatosis in liver biopsies from an older patient. Lastly, super-resolution microscopy allowed us to visualize and determine the size distribution of fenestrations in human liver sinusoidal endothelial cells for the first time under aqueous conditions. Thus, this proof-of-concept study allows us to demonstrate, how, in combination, these techniques open up a new chapter in liver biopsy analysis.

Long-Distance Ultrafast Spin Transfer over a Zigzag Carbon Chain Structure.

Using high-level ab initio quantum theory we suggest an optically induced subpicosecond spin-transfer scenario over 4.428 nm, a distance which is directly comparable to the actual CMOS scale. The spin-density transfer takes place between two Ni atoms and over a 40-atom-long zigzag carbon chain. The suitable combination of the local symmetries of the participating carbon atoms and the global symmetry of the whole molecule gives rise to what we term the dynamical Goodenough-Kanamori rules, allowing the long-range coupling of the two Ni atoms. We also present local spin-flip scenarios, and compare spin flip and spin transfer with respect to their sensitivity against an external static magnetic gradient. Finally, we use two identical laser pulses, rather than a single one, which allows us to accurately control local (intrasite) vs global (intersite) processes, and we thus solve the problem of embedding individually addressable molecular nanologic elements in an integrated nanospintronic circuit. Our results underline the great potential of carbon chain systems as building and supporting blocks for designing future all-optical magnetic processing units.

A Replication-Competent HIV Clone Carrying GFP-Env Reveals Rapid Env Recycling at the HIV-1 T Cell Virological Synapse.

HIV-1 infection is enhanced by cell-cell adhesions between infected and uninfected T cells called virological synapses (VS). VS are initiated by the interactions of cell-surface HIV-1 envelope glycoprotein (Env) and CD4 on target cells and act as sites of viral assembly and viral transfer between cells. To study the process that recruits and retains HIV-1 Env at the VS, a replication-competent HIV-1 clone carrying an Env-sfGFP fusion protein was designed to enable live tracking of Env within infected cells. Combined use of surface pulse-labeling of Env and fluorescence recovery after photobleaching (FRAP) studies, enabled the visualization of the targeted accumulation and sustained recycling of Env between endocytic compartments (EC) and the VS. We observed dynamic exchange of Env at the VS, while the viral structural protein, Gag, was largely immobile at the VS. The disparate exchange rates of Gag and Env at the synapse support that the trafficking and/or retention of a majority of Env towards the VS is not maintained by entrapment by a Gag lattice or immobilization by binding to CD4 on the target cell. A FRAP study of an Env endocytosis mutant showed that recycling is not required for accumulation at the VS, but is required for the rapid exchange of Env at the VS. We conclude that the mechanism of Env accumulation at the VS and incorporation into nascent particles involves continuous internalization and targeted secretion rather than irreversible interactions with the budding virus, but that this recycling is largely dispensable for VS formation and viral transfer across the VS.

Platelets Boost Recruitment of CD133+ Bone Marrow Stem Cells to Endothelium and the Rodent Liver-The Role of P-Selectin/PSGL-1 Interactions.

We previously demonstrated that clinical administration of mobilized CD133+ bone marrow stem cells (BMSC) accelerates hepatic regeneration. Here, we investigated the potential of platelets to modulate CD133+BMSC homing to hepatic endothelial cells and sequestration to warm ischemic livers. Modulatory effects of platelets on the adhesion of CD133+BMSC to human and mouse liver-sinusoidal- and micro- endothelial cells (EC) respectively were evaluated in in vitro co-culture systems. CD133+BMSC adhesion to all types of EC were increased in the presence of platelets under shear stress. This platelet effect was mostly diminished by antagonization of P-selectin and its ligand P-Selectin-Glyco-Ligand-1 (PSGL-1). Inhibition of PECAM-1 as well as SDF-1 receptor CXCR4 had no such effect. In a model of the isolated reperfused rat liver subsequent to warm ischemia, the co-infusion of platelets augmented CD133+BMSC homing to the injured liver with heightened transmigration towards the extra sinusoidal space when compared to perfusion conditions without platelets. Extravascular co-localization of CD133+BMSC with hepatocytes was confirmed by confocal microscopy. We demonstrated an enhancing effect of platelets on CD133+BMSC homing to and transmigrating along hepatic EC putatively depending on PSGL-1 and P-selectin. Our insights suggest a new mechanism of platelets to augment stem cell dependent hepatic repair.