Variability of the essential oil from three sorts of Echinacea MOENCH genus during ontogenesis.

Variability of the essential oil from three sorts of Echinacea MOENCH genus during ontogenesis The content and quality of the essential oil in relation to the main ontogenetic stages of plants were studied in three various sorts of Echinacea genus. The comparison included Echinacea purpurea, Echinacea atrorubens, and Echinacea pallida. The differences in the content of the oil in different parts of plants and the abundance of individual oil constituents in oils from the sorts under study at the optimum stage of ripeness for harvest were evaluated as well.

Classification of non-bacterial osteitis: retrospective study of clinical, immunological and genetic aspects in 89 patients.

OBJECTIVE: To define non-bacterial osteitis (NBO) as a clinical entity possibly associated with autoimmune manifestations. Patients with sterile osteitis were analysed to develop diagnostic criteria. METHODS: A total of 89 patients with non-bacterial inflammatory bone lesions were observed for a median of 49 months. History, diagnostic imaging, laboratory and histological data were obtained. Mutation analysis in the genes PSTPIP1 and PSTPIP2 was performed. RESULTS: Patients had an onset of disease at a median age of 10 yrs [interquartile range (IQR) 7.5-12] and suffered a median period of 21 (IQR 9-52) months with a median of three foci per patient. Twenty percent of all the patients demonstrated associated autoimmune disorders, particularly of the skin and bowel. The majority of bone lesions were located in the vertebrae and metaphyses. Slight-to-moderate elevation of inflammation values were found in all the patients and antinuclear antibodies were elevated in 30%. Non-steroidal anti-inflammatory drugs (NSAIDs) were effective in 85% of the patients. HLA-B27 and Human Leukocyte Antigen-DR (HLA-DR)-classification did not differ from the general population. Autoimmune diseases in 40% of all the families, multiply affected family members, linkage to 18q21 and mouse models strongly indicate a genetic basis for NBO. We observed three different courses of disease regarding the duration of complaints, rate of complications and associated autoimmune manifestations leading to a new classification of NBO. CONCLUSIONS: Clinical analysis of our cohort leads us to define NBO as a distinct disease entity with three clinical presentations: acute NBO, chronic recurrent multifocal osteomyelitis or persistent chronic NBO. Diagnostic criteria were proposed to differentiate NBO from diseases with similar clinical presentation.

[A study of qualitative properties of the essential oil of Tanacetum vulgare L]. / Studium kvalitatívnych vlastností silice Tanacetum vulgare L.

The study examined the qualitative properties of the essential oil obtained from the flower heads of the species Tanacetum vulgare L. The composition of the essential oil was evaluated in plants from various localities, which differed in the altitude, temperature gradient, as well as soil-climate conditions. With the use of gas chromatography (GC/MS), 49 constituents of the essential oil were separated, out of which 35 was identified. The shares of the constituents in the essential oil differed in dependence on the locality. In the lowest-lying locality with a relatively large sunshine, the content of beta-tujons in the essential oil was the highest, whereas in the regions lying towards the north the content of the essential oil was decreased and the content of camphor was increased. In the northern most region an increased number of chemovars of the camphor-cineole type was observed. In conclusion, it must be said that different climatic and agrotechnical conditions, geographical origin and adaptation of the genotypes of Tanacetum vulgare to the growth conditions can be the causes of the found chemotypes in the localities under examination.