ABSTRACT
In social networks, consumers gather to form brand communities, and the community structure significantly impacts the dissemination of brand information. Which communication strategy is more conducive to information dissemination in different structured brand communities? Considering the above factors, we propose the word-of-mouth (WOM) agent model based on the traditional rumor model and bass model, in which the brand WOM spreading is affected by the user's psychological mechanisms, the network structure, and other factors. Through simulation experiments, the results showed the following: (1) the conclusion of the traditional bass model is no longer applicable to social marketing in brand information diffusion, that is, the effect of external marketing stimulation on information dissemination is limited. (2) The communication effect and the efficiency of information in different structures of the learning-community network are very different. (3) The strategy of hub nodes is not suitable for all types of networks, and the impact of different seeding strategies on the efficiency and effect of brand information dissemination was verified. Finally, the conclusion was verified again using the social network data on Facebook.
ABSTRACT
The virtual brand community has become an important marketing tool for companies. A successful brand community marketing strategy should attract a large number of consumers. Although past studies have revealed consumer motivations for participating in virtual brand communities, they fail to answer an important question: Why is it so easy for some virtual brand communities to attract users while others have such difficulty? In this study, product characteristics are hypothesized to be important factors that determine consumer motivation to participate in brand communities. Product characteristics (e.g., product complexity, product symbolism, and product satisfaction) can directly affect how actively consumers participate in brand communities. The results of questionnaires show that product complexity, product symbolism, and product satisfaction have a positive influence on consumers' willingness to participate in brand communities. Notably, the duration of product use has a regulating effect on the influence of product satisfaction and product symbolism. A long period of product use weakens the influence of product satisfaction on consumers' willingness to participate in brand communities. On the contrary, a long period of product use strengthens the influence of product symbolism on consumers' willingness to participate in brand communities. This study enriches the literature on brand community participation and has implications for companies that aim to utilize brand communities for marketing.
ABSTRACT
Objective To investigate the value of next-generation sequencing (NGS) technique for genetic analysis of spontaneous abortion. Methods From January to June 2017, 154 patients who visited the First Affiliated Hospital of Zhengzhou University for spontaneous abortion were enrolled. All abortion tissue samples were analyzed by both NGS combined with short tandem repeat (STR) and single nucleotide polymorphism array (SNP-array). Results of the two methods were compared by Chi-square or Fisher's exact test. Results (1) Chromosomal abnormalities were detected in 109 of the 154 cases (70.7%), including 52 (47.7%) of numerical chromosomal abnormalities, 49 (45.0%) of structural chromosomal abnormalities, six (5.5%) of mosaicism, and two (1.8%) of uniparental disomy (UPD). In those 52 cases of numerical chromosome abnormalities, there were 45 of chromosome aneuploidy and seven of polyploidy. The top three numerical chromosomal abnormalities were 45,X (27.0%, 14/52), trisomy 22 (9.6%, 5/52) and trisomy 16 (7.7%, 4/52). Forty-nine structural abnormality cases carried 67 copy number variations (CNV), including 13 pathogenic CNV (pCNV, 19.4%), 24 variants of unknown clinical significance (35.8%) and 30 benign CNV (44.8%). In those 13 pCNVs, two were responsible for microdeletion and microduplication syndromes. (2) SNP-array was successful in 152 cases, but failed in two (1.3%) due to genomic DNA <200 ng. However, NGS technology was successful in all 154 cases and identified chromosomal abnormalities in the two cases that SNP-array had failed. No statistically significant difference was shown in the detection rate of chromosomal abnormalities between SNP-array and NGS technology [70.4% (107/152) vs 67.5% (104/154), χ2=0.293, P=0.588]. (3) No significant difference in the detection of chromosome aneuploidy (six cases in each group, 3.9% vs 3.9%) and mosaicism (45 cases in each group, 29.2% vs 29.6%) was found between NGS technology and SNP-array. Three cases of polyploidy (69, XXX) and two of UPD were identified by SNP-array, but not by NGS. When combined with STR, NGS was able to detect all three cases of polyploidy (69, XXX). (4) Forty-seven structural abnormality cases detected by SNP-array carried 53 CNVs, and 49 detected by NGS carried 67 CNVs. (5) NGS detected ten, three and one more CNVs than SNP-array did when the genome lengths were 100-<500, 500-<1 000 and ≥1 000 kb, respectively. Conclusions NGS can be used to detect chromosomal aneuploidy and mosaicism that can be identified by SNP-array with fewer limitations on total amount of genome. Moreover, CNVs that fail to be identified by SNP-array can also be detected by NGS. When combined with STR, NGS can effectively detect chromosomal polyploidy. Therefore, NGS could be a potential genetic analysis method for spontaneous abortion and of importance for genetic counseling.
ABSTRACT
Objective The article aimed to investigate the effect of application of phase learning teaching method in teaching of clinical nursing skills.Methods A total of 97 undergraduate nursing students in grade 2008 were grouped as the experimental group,these students adopted phase learning teaching method,which divided clinical nursing skills course into three stages.A total of 103 undergraduate nursing students in grade 2008 were set as the control group,the students were given traditional teaching method.The teaching effect was compared between two groups.Results Compared with the control group,theoretical knowledge,practical skills,professional emotion and critical thinking were better in the experimental group,the difference was significant,t value was-4.566,-6.332,-2.371 and-4.308.Conclusions The phase learning teaching method is an effective and practical method to improve the teaching effect of clinical nursing skills.
