ABSTRACT
Objective To study the relationship between chemotherapeutic resistance to 5-fluorouracil (5-FU) and Golgi phosphoprotein 3 (GOLPH3) expression in human colon cancer cell line HT29.Methods HT29 cells were divided into four groups:control group,siRNA transfection group,experimental group 1 (30 μmol/L 5-FU),and experimental group 2 (siRNA-GOLPH3 + 30 μmol/L 5-FU).The silencing effect of GOLPH3 gene was detected by RT-PCR and Western blot.The tumor proliferation and formation ability of HT29 cell were measured respectively by MTT and plate colony formation assay.The protein expressions of GOLPH3,P-gp and β-catenin in HT29 cell were detected by Western blot.Results Compared with the control group,the expression level of GOLPH3 mRNA (1.000 ± 0.078 vs.0.147 ± 0.021,t =12.296,P ＜ 0.01) and protein(1.003 ± 0.235 vs.0.077 ± 0.399,t =6.723,P ＜ 0.01),the absorbance(A value) (1.000 ± 0.082 vs.0.769 ± 0.086,t =3.885,P ＜ 0.01) and the tumorigenesis (430 ±36 vs.297 ± 21,t =5.492,P ＜ 0.01) in transfection group significantly reduced.The A value (0.803 ± 0.101 vs.1.050 ± 0.140,t =2.855,P ＜ 0.05;0.242 ± 0.091 vs.1.050 ± 0.140,t =9.664,P ＜ 0.001) and the tumorigenesis (73 ± 8 vs.427 ± 29,t =20.363,P ＜ 0.001;305 ± 22 vs.427 ± 29,t =5.840,P ＜ 0.01) in experimental group 1 and experimental group 2 were significantly lower than that in the control group,while the A value (t =8.264,P ＜ 0.001) and the tumorigenesis (t =17.346,P ＜ 0.001) of experimental group 2 significantly decreased than that experimental group 1.Compared to the control group (0.967 ± 0.094,1.001 ± 0.199,1.000 ± 0.101),the expressions of GOLPH3,P-gp and β-catenin in experimental group 1 (3.634 ± 0.574,2.424 ± 0.261,2.324 ± 0.418) significantly upregulated (t =7.944,P ＜ 0.01;t =7.520,P ＜ 0.01;t =5.330,P ＜ 0.01),while the expressions of these proteins in experimental group 2(0.520 ±0.176,0.466 ±0.159,0.933 ±0.049) were significantly lower than that in experimental group 1 (t =8.983,P ＜ 0.01;t =11.106,P ＜ 0.001;t =5.724,P ＜ 0.01).Conclusion Expression of GOLPH3 is associated with resistance to 5-FU in colon cancer HT29 cell by activating Wnt/β-catenin signal pathway.
ABSTRACT
Objective@#To evaluate the efficacy of WEI NASAL JET for supraglottic ventilation before tracheal intubation in the patients with tooth loss.@*Methods@#Sixty patients of both sexes with tooth loss (more than 8 teeth missing), aged 67-83 yr, of American Society of Anesthesiologists physical statusⅠ-Ⅲ, with Mallampati classificationⅠ-Ⅲ, with body mass index of 18-25 kg/m2, undergoing surgery with general anesthesia, were divided into 2 groups (n=30 each) using a random number table method: WEI NASAL JET group (S group) and mask group (M group). After the end of anesthesia induction, WEI NASAL JET was inserted via the nasal cavity to perform supraglottic ventilation in group S, and two-hand buckle mask was performed in group M, and the patients were tracheally intubated after 5-min ventilation.At 5-min spontaneous breathing after nitrogen removal by oxygen supply, 1, 2, 3 and 4 min after no spontaneous breathing and immediately after intubation (T5), ultrasound was used to measure the amplitude of diaphragm motion induced by respiratory movement, and blood gas analysis was performed to record the development of pH value<7.30, SpO2<90% and fluctuation in mean arterial pressure and heart rate ≥30% of baseline before operation.The development of ventilation-related complications was also recorded.@*Results@#Compared with group M, the amplitude of diaphragm motion induced by respiratory movement was significantly increased at T1-5, PaO2was increased at T2-5, PaCO2 and PETCO2 were decreased at T3-5, the incidence of sore throat and nasal mucosal bleeding was increased, the incidence of gingiva injury and flatulence was decreased (P<0.05), and no significant change was found in the incidence of fluctuation in mean arterial pressure and heart rate ≥30% of baseline in group S (P>0.05). The pH value<7.30 and SpO2<90% were not found in two groups.@*Conclusion@#WEI NASAL JET can provide satisfactory supraglottic ventilation efficacy before tracheal intubation with good safety in the patients with tooth loss.
ABSTRACT
Objective To evaluate the efficacy of WEI NASAL JET for supraglottic ventilation before tracheal intubation in the patients with tooth loss.Methods Sixty patients of both sexes with tooth loss (more than 8 teeth missing),aged 67-83 yr,of American Society of Anesthesiologists physical status Ⅰ-Ⅲ,with Mallampati classification Ⅰ-Ⅲ,with body mass index of 18-25 kg/m2,undergoing surgery with general anesthesia,were divided into 2 groups (n =30 each) using a random number table method:WEI NASAL JET group (S group) and mask group (M group).After the end of anesthesia induction,WEI NASAL JET was inserted via the nasal cavity to perform supraglottic ventilation in group S,and two-hand buckle mask was performed in group M,and the patients were tracheally intubated after 5-min ventilation.At 5min spontaneous breathing after nitrogen removal by oxygen supply,1,2,3 and 4 min after no spontaneous breathing and immediately after intubation (T5),ultrasound was used to measure the amplitude of diaphragm motion induced by respiratory movement,and blood gas analysis was performed to record the development of pH value＜7.30,SpO2＜90％ and fluctuation in mean arterial pressure and heart rate ≥30％ of baseline before operation.The development of ventilation-related complications was also recorded.Results Compared with group M,the amplitude of diaphragm motion induced by respiratory movement was significantly increased at T1-5,PaO2was increased at T2-5,PaCO2 and PETCO2 were decreased at T3-5,the incidence of sore throat and nasal mucosal bleeding was increased,the incidence of gingiva injury and flatulence was decreased (P ＜ 0.05),and no significant change was found in the incidence of fluctuation in mean arterial pressure and heart rate ≥30％ of baseline in group S (P＞0.05).The pH value＜7.30 and SpO2＜90％ were not found in two groups.Conclusion WEI NASAL JET can provide satisfactory supraglottic ventilation efficacy before tracheal intubation with good safety in the patients with tooth loss.
