Endangered island endemic plants have vulnerable genomes.

Loss of genetic diversity is known to decrease the fitness of species and is a critical factor that increases extinction risk. However, there is little evidence for higher vulnerability and extinction risk in endangered species based on genomic differences between endangered and non-endangered species. This is true even in the case of functional loci, which are more likely to relate to the fitness of species than neutral loci. Here, we compared the genome-wide genetic diversity, proportion of duplicated genes (PD), and accumulation of deleterious variations of endangered island endemic (EIE) plants from four genera with those of their non-endangered (NE) widespread congeners. We focused on exhaustive sequences of expressed genes obtained by RNA sequencing. Most EIE species exhibited significantly lower genetic diversity and PD than NE species. Additionally, all endangered species accumulated deleterious variations. Our findings provide new insights into the genomic traits of EIE species.

Differential expression of three types of gonadotropin-releasing hormone genes during the spawning season in grass puffer, Takifugu niphobles.

Grass puffer, Takifugu niphobles, has unique spawning behavior; spawning occurs on beach only for several days around new moon and full moon from spring to early summer. To investigate the role of gonadotropin-releasing hormone (GnRH) in the reproductive function, genes encoding three types of GnRHs, namely seabream GnRH (sbGnRH), chicken GnRH-II (cGnRH-II) and salmon GnRH (sGnRH), were cloned and changes in their mRNA amounts were examined over the spawning season. In addition, changes in the pituitary gonadotropin subunit mRNAs and the plasma steroid hormones were examined over the spawning season. Fishes were assessed at four reproductive stages, i.e., in December (early maturation), in April (maturing), in May (spawning), and in July (post-spawning). Moreover, spawning fish just after releasing eggs and sperm were taken at a spawning bed. The amounts of sbGnRH mRNA were substantially elevated in May and the spawning fish in both sexes, concomitant with considerable elevations of follicle-stimulating hormone and luteinizing hormone beta subunit mRNAs and plasma estradiol-17beta (E(2)) and testosterone (T) levels. There were strong positive correlations between the sbGnRH mRNA and the plasma E(2) and T levels over the spawning season in both sexes. The amounts of cGnRH-II mRNA showed no noticeable changes except for an increase in the post-spawning females. The amounts of sGnRH mRNA in the males were significantly increased in May, but they were low in the spawning males. In the females, sGnRH mRNA increased from the maturing stage and reached a maximum in the post-spawning stage, in which a positive correlation with the plasma cortisol levels was observed. These specific changes suggest that the expression of three types of GnRH genes is differentially regulated during the spawning season, and sex steroids may be important for the differential expression of GnRH genes.

Structure of neurohypophysial hormone genes and changes in the levels of expression during spawning season in grass puffer (Takifugu niphobles).

Vasotocin (VT) has been shown to influence various aspects of social and sexual behaviors in a broad range of vertebrate species, but less is known about the mechanisms through which this peptide modulates behavior. Additionally, much less is known about roles of isotocin (IT) in regulation of behavior. Grass puffer, Takifugu niphobles, has unique spawning behavior; spawning occurs on beach only for several days around the spring tide and is conducted by a group of 10-60 individuals, of which one is female. As a first step toward investigating the roles of VT and IT in this species' spawning behavior, we determined the structures of the VT and IT genes from grass puffer using the genome resources of the closely related tiger puffer and green puffer. We then used these sequences to develop real-time PCR assays and examined changes in expression of the VT and IT genes over the spawning season. The structures of VT and IT genes are well conserved among three puffer species. Particularly, the sequence similarities between grass and tiger puffers were very high not only in the coding region (85-99%), but also in the non-coding regions (92-98%) that include the 5'-upstream regions. The levels of expression of VT gene increased in the brain of pre-spawning females. The levels of VT mRNA in the spawning females tended to be higher than that in the spawning males. In contrast, the levels of IT mRNA did not show such variation. The present results suggest that VT gene expression augments in the brain of females during the spawning period. The unique spawning behavior of grass puffer provides a useful model for studying the molecular mechanism of sexual behavior utilizing the genome resources of tiger puffer.