Porphyromonas gingivalis can degrade dental zirconia.

OBJECTIVES: The aim of present study was to examine the effect of Porphyromonas gingivalis (P.g.) adhesion on dental zirconia by characterizing the physical and chemical properties. METHODS: Eighty polished-sintered zirconia discs were prepared and randomly distributed to 5 groups (n = 16): Zirconia cultured with - Group 1: broth containing P.g. for - 3 days; Group 2: 7 days; Group 3: broth (alone) for - 3 days; Group 4: 7 days; and Group 5: dry discs (negative control). After experimental period, broths were analyzed for pH and Zr release with inductively coupled plasma-optical emission spectroscopy (ICP-OES). The zirconia surface was evaluated by scanning electron microscope (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), water contact angle (WCA), and biaxial flexural strength (BFS). RESULTS: The mean pH with zirconia adhesion to P.g. group was significantly higher than the broth control (p < 0.05). As per ICP-OES, Zr ion/particulate release with P.g. adhesion to zirconia were significantly higher than the controls (p < 0.05). Post-experimental incubation, no defects were found on zirconia surfaces; tetragonal phase remained constant with no transformation to monoclinic phase but lower peak intensities were identified in experimental groups. WCA of zirconia surfaces with P.g. bacteria for 3 days (12.04° ± 2.05°) and 7 days (15.09° ± 2.95°) were significantly higher than zirconia surfaces immersed with broth (only) for 3 days (7.17° ± 1.09°) and 7 days (7.55° ± 0.65°), respectively (p < 0.05). BFS values of zirconia with P.g. for 3 days (632.57 ± 119.96 MPa) and 7 days (656.17 ± 100.29 MPa) were significantly lower than zirconia incubated in broth alone (765.01 ± 20.12 MPa) conditions (p < 0.05). SIGNIFICANCE: Under the conditions of present study, it can be concluded that P.g. adhesion on zirconia leads to structural alterations of dental zirconia further contributing to zirconia degradation.

An introduction of biological performance of zirconia with different surface characteristics: A review.

Zirconia (ZrO2) ceramic is widely used in dentistry as a clinical dental biomaterial. In this review, we are focusing on and summarizing the biological performance of zirconia under different surface characteristics. We have included an initial tissue cell attachment study on zirconia and bacterial adhesion on zirconia. Our results suggest that surface modifications applied on zirconia may change the interfacial surface characteristics e.g. surface roughness, surface free energy, and chemistry of zirconia. The modifications also result in advanced biological performance of zirconia, including enhanced tissue cell attachment and reduction of bacterial adhesion. The recent laboratory research has provided many interesting modification methods and showed clinically interesting and promising outcomes. A few of the outcomes are validated and have been applied in clinical dentistry.

Prolonged UV-C Irradiation is a Double-Edged Sword on the Zirconia Surface.

Zirconia has become an excellent choice of dental implants because of its excellent mechanical strength, aesthetic, and biocompatibility. Although some studies have shown ultraviolet (UV) irradiation is effective to photofunctionalize dental zirconia that can improve osteoblastic function, the scattered information has not identified the most effective exposure time and wavelength of UV. Herein, this study has investigated the effects of UV irradiation on zirconia after UV-A (365 nm) or UV-C (243 nm) photofunctionalization for different times (15 min, 3 and 24 h). After irradiation, the zirconia surface was analyzed by color spectrophotometry, scanned electron microscopy (SEM), energy-dispersive X-ray spectrometry, water contact angle (WCA) with goniometer, and X-ray diffraction. Osteoblastic (MC3T3-E1) cells were cultured on zirconia discs and evaluated with a CCK-8 test kit for cell proliferation (3 h and 1 day) and with alkaline phosphatase (ALP) activity (14 days). Significant color change (ΔE) was observed by irradiating with UV-C for 15 min (1.99), 3 h (1.92), and 24 h (3.35), whereas only minute changes were observed with UV-A (respectively, ΔE: 0.18, 0.14, 0.57). No surface textural changes were observed nor a monoclinic phase was detected on both the UV-A and UV-C irradiated samples. UV-C significantly decreased the C/Zr ratios and WCA, with irradiating for 24 h presenting the lowest values, and it was the only condition to give significantly higher ALP activity at 14 days (p < 0.05) and CCK-8 values for 1 day culture (p < 0.05). It is concluded that UV-C (but not UV-A) irradiation can significantly change the aesthetic in color, and only prolonged 24 h UV-C irradiation can enhance MC3T3-E1 cell adhesion on zirconia by photofunctionalization.

Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro.

OBJECTIVE: The current laboratory study was to investigate the effect of different sterilization treatments on surface characteristics of zirconia, and biofilm formation on zirconia surface after exposure to these sterilization treatments. METHODS: Commercially available zirconia discs (Cerconbase, Degu-Dent, Hanau, Germany) were prepared and polished to the same value of surface roughness. The discs were treated with one of the following sterilization methods steam autoclave sterilization, dry heat sterilization, ultraviolet C (UVC) irradiation, and gamma (γ) ray irradiation. The characteristics of zirconia surfaces were evaluated by scanning electron microscopy (SEM), surface roughness, surface free energy (SFE), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) measurements. Then, Staphylococcus aureus (S.a.) and Porphyromonas gingivalis (P.g.) bacteria were used and cultured on the respective sterilized zirconia surfaces. The amount of biofilm formation on zirconia surface was quantified by colony forming unit (CFU) counts. RESULTS: Significant modifications were detected on the colour and SFE of zirconia. The colour of zirconia samples after UVC irradiation became light yellow whilst dark brown colour was observed after gamma ray irradiation. Moreover, UVC and gamma ray irradiation increased the hydrophilicity of zirconia surface. Overall, dry heat sterilized samples showed the significantly lowest amount of bacteria growth on zirconia, while UVC and gamma ray irradiation resulted in the highest. SIGNIFICANCE: It is evident that various sterilization methods could change the surface which contribute to different biofilm formation and colour on zirconia.