The role of the SIRT1-BMAL1 pathway in regulating oxidative stress in the early development of ischaemic stroke.

Oxidative stress is the primary cause of ischaemic stroke and is closely related to circadian rhythm. However, the mechanism by which circadian rhythm regulates oxidative stress in ischaemic stroke remains elusive. The Silent Information Regulator 1 (SIRT1) controls circadian rhythm by activating the transcription of the circadian clock core protein Basic Helix-Loop-Helix ARNT Like 1 (BMAL1) through deacetylation. Studies have shown that the SIRT1-BMAL1 pathway can regulate oxidative stress. To investigate its correlation with oxidative stress, we examined the expression levels and influencing factors of SIRT1-BMAL1 at different times in ischaemic stroke patients and analyzed their clinical indexes, oxidative stress, and inflammatory factor indicators. The expression levels of oxidative stress and inflammatory factor indicators, including malondialdehyde (MDA), superoxide dismutase (SOD), interleukin-6 (IL-6), and tumor necrosis factor-a (TNF-α), SIRT1, and BMAL1, were detected in ischaemic stroke patients within 4.5 h of onset and in non-stroke patients. Patients were divided into four subgroups based on onset time: subgroup 1 (0:00-05:59); subgroup 2 (06:00-11:59); subgroup 3 (12:00-17: 59); and subgroup 4 (18:00-23:59). Our results showed higher MDA, IL-6, and TNF-α levels, and lower SOD, SIRT1, and BMAL1 levels in ischaemic stroke patients compared to control patients (P < 0.05). Among the four subgroups, the content of MDA, IL-6, and TNF-α was highest in patients with ischaemic stroke onset from subgroup 2 (06:00-11:59), while the expression levels of SOD, BMAL1, and SIRT1 were lowest in patients with ischaemic stroke in subgroup 2. Additionally, myeloperoxidase (MPO) reached the highest value showing the same trends consistent with MDA, IL-6, and TNF-ɑ and opposite trends consistent with SOD, BMAL1, and SIRT1. However, triglycerides (TGs), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), immediate blood glucose, immediate diastolic blood pressure, immediate systolic blood pressure, and homocysteine (HCY) did not show any statistically significant circadian rhythm changes (P > 0.05). Our findings suggest that the SIRT1-BMAL1 pathway may be involved in early oxidative stress in ischaemic stroke, which may be related to MPO.

Dynamics of high-contrast grating vertical-cavity surface-emitting laser with lateral optical feedback by a heterostructure interface.

We investigate the dynamics of high-contrast grating vertical-cavity surface-emitting laser (HCG-VCSEL) with a lateral optical feedback cavity. The lateral optical feedback is realized by the reflection at the heterostructure interface between two different HCGs. The lateral optical feedback cavity possesses slow light which can be tuned by changing HCG parameters, and can control the dynamics of the HCG-VCSEL. The optical feedback can enhance the -3-dB bandwidth and enlarge the eye openings of diagrams of the HCG-VCSEL, and can also reduce the frequency chirp. The HCG-VCSEL with a lateral optical feedback cavity can achieve a -3-dB bandwidth of 37.7 GHz at 12 mA and eye diagrams at 60 Gbps (non-return to zero format) and 50 GBaud (4-level pulse amplitude modulation format) with sufficient openings.