ABSTRACT
BACKGROUND AIMS: The production of commercial autologous cell therapies such as chimeric antigen receptor T cells requires complex manual manufacturing processes. Skilled labor costs and challenges in manufacturing scale-out have contributed to high prices for these products. METHODS: We present a robotic system that uses industry-standard cell therapy manufacturing equipment to automate the steps involved in cell therapy manufacturing. The robotic cluster consists of a robotic arm and customized modules, allowing the robot to manipulate a variety of standard cell therapy instruments and materials such as incubators, bioreactors, and reagent bags. This system enables existing manual manufacturing processes to be rapidly adapted to robotic manufacturing, without having to adopt a completely new technology platform. Proof-of-concept for the robotic cluster's expansion module was demonstrated by expanding human CD8+ T cells. RESULTS: The robotic cultures showed comparable cell yields, viability, and identity to those manually performed. In addition, the robotic system was able to maintain culture sterility. CONCLUSIONS: Such modular robotic solutions may support scale-up and scale-out of cell therapies that are developed using classical manual methods in academic laboratories and biotechnology companies. This approach offers a pathway for overcoming manufacturing challenges associated with manual processes, ultimately contributing to the broader accessibility and affordability for personalized immunotherapies.
ABSTRACT
BACKGROUND: Collaboration between community and healthcare professionals plays an essential role in ADHD care. Therefore, this study aims to identify the level of knowledge/understanding, perception and attitude among community members, primary school teachers, medical students, general practitioners, pediatricians and psychologists in Indonesia regarding ADHD and its association with several related factors. METHODS: A cross-sectional study utilizing valid questionnaires to assess the level of knowledge/understanding, perception and attitude regarding ADHD among each respondent group was employed. 1,536 subjects participated in this study. Data was gathered and analyzed using the SPSS version 20.0 for Mac. A chi-square test was used to establish the associations. The value of p < 0.05 was considered statistically significant in this study. RESULTS: Most of the participants showed poor to very poor levels of knowledge/understanding (community members = 56.8%, teachers = 58.9%, medical students = 60.4%, general practitioners = 54.9%, pediatricians = 65.6%, psychologists = 52.1%). The similar results were also found for perception and attitude levels. Several associations were found between practice experience and the level of knowledge/understanding, perception and attitude towards ADHD among general practitioners and psychologists (p < 0.05); educational level and level of attitude among community members and medical students (p < 0.05); practice experience and the knowledge/understanding level in the teachers group (p < 0.05); practice experience and the perception level among pediatricians (p < 0.05). CONCLUSIONS: Further education regarding ADHD should be considered either through online media or formal education in order to achieve a better management of ADHD.
