Frequency shift of parametric sound by face-to-face pair of sources in relative motion.

This paper reports an acoustic phenomenon regarding a parametric sound source (also referred to as a parametric array): a secondary sound wave is generated from the nonlinear interaction of multiple primary sound waves with varied frequency components, particularly when two relatively moving sound sources face each other. It was found that the frequency of the secondary wave fluctuated according to the source movement and provided a theoretical explanation for this phenomenon. It is experimentally demonstrated that this frequency shift was approximately proportional to the velocity of the moving source toward the fixed source and to the driving frequency of the moving source. This phenomenon has much in common with the Doppler's effect, but its unique property is that the frequency shift depends on neither the observation position nor the source velocity toward the observer. These sound generation principles enable measurement of the velocity of slowly moving sound sources while maintaining a low-modulation frequency band and a short measurement time. This phenomenon can potentially be applied to an alternative approach for acoustic noncontact velocimetry of moving objects.

Self-localization of monaural microphone using dipole sound sources.

This paper introduces a method for indoor self-localization of a monaural microphone, which is required for various location-based services. By generating two pairs of dipole sound fields, localization is performed on each device, irrespective of the number of devices, based on orthogonal detection of observed signals and some simple operations that are feasible with limited computational resources. A method using multiple source frequencies for enhancing robustness against the effects of reflection and scattering is also proposed. The effectiveness of this method was evaluated by numerical simulations and experiments in an anechoic chamber and indoor environment, and the average errors for the azimuth and zenith angles were 4.8 and 1.9 deg, respectively, in the anechoic chamber and 21 and 11 deg, respectively, in the indoor environment.

A Method for Electrical Property Tomography Based on a Three-Dimensional Integral Representation of the Electric Field.

Magnetic resonance electrical properties tomography (MREPT) noninvasively reconstructs high-resolution electrical property (EP) maps using MRI scanners and is useful for diagnosing cancerous tissues. However, conventional MREPT methods have limitations: sensitivity to noise in the numerical Laplacian operation, difficulty in reconstructing three-dimensional (3D) EPs and convergence not guaranteed in the iterative process. We propose a novel, iterative 3D reconstruction MREPT method without a numerical Laplacian operation. We derive an integral representation of the electric field using its Helmholtz decomposition with Maxwell's equations, under the assumption that the EPs are known on the boundary of the region of interest with the approximation that the unmeasurable magnetic field components are zero. Then, we solve the simultaneous equations composed of the integral representation and Ampere's law using a convex projection algorithm whose convergence is theoretically guaranteed. The efficacy of the proposed method was validated through numerical simulations and a phantom experiment. The results showed that this method is effective in reconstructing 3D EPs and is robust to noise. It was also shown that our proposed method with the unmeasurable component H- enhances the accuracy of the EPs in a background and that with all the components of the magnetic field reduces the artifacts at the center of the slices except when all the components of the electric field are close to zero.

Theoretical approach for modeling LSPR biosensors for the detection of biopolymer nucleation.

We investigate theoretically the accuracy of an effective medium approximation in modeling localized surface plasmon resonance biosensors for the detection of biopolymer nucleation. Using boundary-element method simulations, we compute the extinction spectrum and spectral shift of a gold nanoparticle-biopolymer system in which biopolymers are treated as cylindrical rods and compare them with results from an extended Mie theory in which biopolymers are treated as an effective medium with a radially variable refractive index. Our results show that the effective medium approximation can be employed to predict the extinction spectrum and the spectral shift accurately. We also demonstrate that the approximation is effective even when there is only a single biopolymer on the nanoparticle, as long as its orientation relative to the incident wave is random, which is generally the case in solution-based localized surface plasmon resonance biosensing applications where the orientations of biomolecules relative to the incident light are not fixed, but change over time.

Spatiotemporal Pinpoint Cooling Sensation Produced by Ultrasound-Driven Mist Vaporization on Skin.

In this study, we achieved a noncontact tactile display that presents a pinpoint and instantaneous cooling sensation on the skin surface with no devices directly in contact with the user's body. We employed ultrasound phased arrays to generate a focused ultrasound, which locally and instantaneously expedites the vaporization of room-temperature water mist floating near the surface of the user's skin, offering a sudden pinpoint cooling sensation. In this article, we describe the physical configuration of the proposed method and show the measurement results, demonstrating how the user's skin surface was cooled. During the experiments, we discovered that a part of the skin exposed to a focused ultrasound within the floating mist was selectively cooled with negligible delay. Our prototype system offers a cooling spot of approximately 15 mm in diameter, which causes a temperature decrease of 4.6 K in 1 s and 3.3 K in the first 0.5 s on a hand situated 500 mm away from the device. Additionally, the ultrasound-driven cooling spot can be controlled on the skin surface, which is felt as a cool moving spot. Such a position-free cooling system with a high spatiotemporal resolution will open the door to unprecedented practical tactile applications.

Sequential structured volumetric ultrasound holography for self-positioning using monaural recording.

In this article, a structured acoustic holography technique in the self-positioning method of a single microphone from the monaurally recorded signals is proposed. A series of three-dimensional ultrasonic holograms, designed for positioning in a workspace, are sequentially projected. As a result, the microphone receives a position-dependent sequence of amplitude signals encoded with information on the observation position. Subsequently, the microphone position is determined by obtaining the peak position of the cross-correlation function between the received signal and the reference signal. Experiments were conducted using a custom-made phased array of 40-kHz ultrasound transducers to evaluate the positioning accuracy. It is demonstrated that when applied to a 100×100×50 mm3 workspace, the measurement error was less than 1 mm at all observation points in the numerical experiment, which was maintained for more than 96% of the points in the real-environment experiments. The proposed method is advantageous in that it does not use the phase information of the recorded signals, thus requiring no multiple synchronized recordings as the microphone-array-based methods. In addition, this scheme does not directly use the absolute value of the received amplitude as a positioning clue, which means that no amplitude-to-voltage calibration is required.

Tactile Stimulation by Repetitive Lateral Movement of Midair Ultrasound Focus.

We report a new vibrotactile modulation method of midair ultrasound focus, namely, lateral modulation (LM), in which the focus quickly moves along a small cyclic trajectory and provides stronger and clearer vibrotactile stimuli than those by the conventional amplitude modulation (AM) method. Midair ultrasound haptics has an essential technical advantage of offering remote, non-contact, and pinpoint tactile stimuli on device-free bare skin. On the other hand, lack of clarity in the presented vibrotactile sensation has often been pointed out, and until now, an AM focus has been valid only on glabrous skin. Our main scientific contribution of the article is to verify the LM method, with the following experimental findings newly obtained. We confirmed that with the same maximum output amplitude of the ultrasound phased arrays, LM stimuli with circular focal trajectories were sensed stronger than AM stimuli by glabrous skin and hairy skin in a modulation frequency of 10-200 Hz. We also found that the detection threshold in glabrous skin mainly depended on the focal speed, whereas the tendency in hairy skin was different from that. With these results, we discuss a basis of perceptional mechanism that responds to LM stimuli, along with practical aspects of potential applications.

Analysis of homogeneous layer approximations in the modeling of localized surface plasmon resonance biosensors.

We analyze theoretically the effectiveness of homogeneous layer approximations in modeling localized surface plasmon resonance biosensors made of spherical metal nanoparticles coated with biomolecular layers that have radially variable refractive indices. Using an extended Mie theory, we compute the extinction spectrum and peak wavelength of the system and compare them with when effective medium approximations are applied to treat the biomolecular layer as homogeneous. We investigate how the accuracies of the approximations depend on the geometric parameters of the system and the material of the metal nanoparticle. We also derive expressions that can be used to predict if the approximations would accurately predict the spectral position of the peak wavelength.

LSPR Biosensing Approach for the Detection of Microtubule Nucleation.

Microtubules are dynamic protein filaments that are involved in a number of cellular processes. Here, we report the development of a novel localized surface plasmon resonance (LSPR) biosensing approach for investigating one aspect of microtubule dynamics that is not well understood, namely, nucleation. Using a modified Mie theory with radially variable refractive index, we construct a theoretical model to describe the optical response of gold nanoparticles when microtubules form around them. The model predicts that the extinction maximum wavelength is sensitive to a change in the local refractive index induced by microtubule nucleation within a few tens of nanometers from the nanoparticle surface, but insensitive to a change in the refractive index outside this region caused by microtubule elongation. As a proof of concept to demonstrate that LSPR can be used for detecting microtubule nucleation experimentally, we induce spontaneous microtubule formation around gold nanoparticles by immobilizing tubulin subunits on the nanoparticles. We find that, consistent with the theoretical model, there is a redshift in the extinction maximum wavelength upon the formation of short microtubules around the nanoparticles, but no significant change in maximum wavelength when the microtubules are elongated. We also perform kinetic experiments and demonstrate that the maximum wavelength is sensitive to the microtubule nuclei assembly even when microtubules are too small to be detected from an optical density measurement.

Midair Haptic Pursuit.

In human vision, smooth pursuit eye movement is the basic ability to visually follow a moving object by keeping it at the sight center. In this study, we validate that a human hand has a similar ability to track a midair haptic stimulus, i.e., a human palm exposed to a point vibration by a noncontact ultrasound tactile display can follow the continuous movement of the stimulation point. The experimental results show that the trackable velocity limit is 10 cm/s for motion parallel to the palm, when the initial velocity is zero. This ability of motion tracking by hand can be applied to haptic guidance for visually impaired people or for evacuation navigation, where no devices are needed to be equipped by users.

Aerial Vibrotactile Display Based on MultiUnit Ultrasound Phased Array.

In this paper, we report on an airborne vibrotactile display with a multiunit ultrasound phased array synthetic aperture. The system generates an ultrasound field with a location-tunable focus in the air, which exerts time-variant acoustic radiation pressure on the user's skin, resulting in perceivable localized vibrotactile stimuli. The paper contains three major new contributions from previous related works. The first is an experimental validation of large-aperture focusing with improved synchronization offering an enlarged workspace in which sufficient acoustic power concentration is guaranteed. From the experiments, it is expected that perceivable vibrotactile focus can be generated 1 m away from a four-unit array system. The second is an experimental evaluation of the presented pressure for producing a broad variety of tactile perception, which shows that the generated ultrasound focus can serve as an vibrotactile actuator that has flat frequency characteristics in the domain of perceptual stimuli. The third is a psychophysical result of the detection threshold curve for sinusoidal stimuli offered by the system. The obtained curve shows similarity with conventionally known results, which have minimum values at approximately 200 Hz.

Midair Ultrasound Fragrance Rendering.

We propose a system that controls the spatial distribution of odors in an environment by generating electronically steerable ultrasound-driven narrow air flows. The proposed system is designed not only to remotely present a preset fragrance to a user, but also to provide applications that would be conventionally inconceivable, such as: 1) fetching the odor of a generic object placed at a location remote from the user and guiding it to his or her nostrils, or 2) nullifying the odor of an object near a user by carrying it away before it reaches his or her nostrils (Fig. 1). These are all accomplished with an ultrasound-driven air stream serving as an airborne carrier of fragrant substances. The flow originates from a point in midair located away from the ultrasound source and travels while accelerating and maintaining its narrow cross-sectional area. These properties differentiate the flow from conventional jet- or fan-driven flows and contribute to achieving a midair flow. In our system, we employed a phased array of ultrasound transducers so that the traveling direction of the flow could be electronically and instantaneously controlled. In this paper, we describe the physical principle of odor control, the system construction, and experiments conducted to evaluate remote fragrance presentation and fragrance tracking.

Resilient Sensor Networks with Spatiotemporal Interpolation of Missing Sensors: An Example of Space Weather Forecasting by Multiple Satellites.

This paper attempts to construct a resilient sensor network model with an example of space weather forecasting. The proposed model is based on a dynamic relational network. Space weather forecasting is vital for a satellite operation because an operational team needs to make a decision for providing its satellite service. The proposed model is resilient to failures of sensors or missing data due to the satellite operation. In the proposed model, the missing data of a sensor is interpolated by other sensors associated. This paper demonstrates two examples of space weather forecasting that involves the missing observations in some test cases. In these examples, the sensor network for space weather forecasting continues a diagnosis by replacing faulted sensors with virtual ones. The demonstrations showed that the proposed model is resilient against sensor failures due to suspension of hardware failures or technical reasons.

RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence.

The coordination of cell cycle progression with the repair of DNA damage supports the genomic integrity of dividing cells. The function of many factors involved in DNA damage response (DDR) and the cell cycle depends on their Ran GTPase-regulated nuclear-cytoplasmic transport (NCT). The loading of Ran with GTP, which is mediated by RCC1, the guanine nucleotide exchange factor for Ran, is critical for NCT activity. However, the role of RCC1 or Ran⋅GTP in promoting cell proliferation or DDR is not clear. We show that RCC1 overexpression in normal cells increased cellular Ran⋅GTP levels and accelerated the cell cycle and DNA damage repair. As a result, normal cells overexpressing RCC1 evaded DNA damage-induced cell cycle arrest and senescence, mimicking colorectal carcinoma cells with high endogenous RCC1 levels. The RCC1-induced inhibition of senescence required Ran and exportin 1 and involved the activation of importin ß-dependent nuclear import of 53BP1, a large NCT cargo. Our results indicate that changes in the activity of the Ran⋅GTP-regulated NCT modulate the rate of the cell cycle and the efficiency of DNA repair. Through the essential role of RCC1 in regulation of cellular Ran⋅GTP levels and NCT, RCC1 expression enables the proliferation of cells that sustain DNA damage.

Character Reading via Stylus Reproducing Normal Handwriting Motion.

In this paper, we report a method of intuitively transmitting symbolic information to untrained users via only their hands, without using any visual or auditory cues. In this simple concept, three-dimensional letter trajectories are presented to the user's hand via a stylus which is mechanically manipulated. In experiments, participants were able to read 14 mm-high lower-case letters displayed at a rate of one letter per second with an accuracy rate of 71.9 percent in their first trials, which improved to 91.3 percent after a 5-minute training period. These results showed small individual differences among participants (standard deviation of 12.7 percent in the first trials and 6.7 percent after training). We also found that this accuracy was still retained to a high level (85.1 percent, with SD of 8.2 percent) even when the letters were reduced to a height of 7 mm. Thus, we revealed that sighted adults potentially possess the ability to read small letters accurately at normal writing speed using their hands.

Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells.

Many mitotic factors were shown to be activated by Ran guanosine triphosphatase. Previous studies in Xenopus laevis egg extracts and in highly proliferative cells showed that mitotic chromosomes were surrounded by steep Ran guanosine triphosphate (GTP) concentration gradients, indicating that RanGTP-activated factors promote spindle assembly around chromosomes. However, the mitotic role of Ran in normal differentiated cells is not known. In this paper, we show that although the steep mitotic RanGTP gradients were present in rapidly growing cell lines and were required for chromosome congression in mitotic HeLa cells, the gradients were strongly reduced in slow-growing primary cells, such as HFF-1 fibroblasts. The overexpression of RCC1, the guanine nucleotide exchange factor for Ran, induced steeper mitotic RanGTP gradients in HFF-1 cells, showing the critical role of RCC1 levels in the regulation of mitosis by Ran. Remarkably, in vitro fusion of HFF-1 cells produced cells with steep mitotic RanGTP gradients comparable to HeLa cells, indicating that chromosomal gain can promote mitosis in aneuploid cancer cells via Ran.

Importazole, a small molecule inhibitor of the transport receptor importin-ß.

During interphase, the transport receptor importin-ß carries cargoes into the nucleus, where RanGTP releases them. A similar mechanism operates in mitosis to generate a gradient of active spindle assembly factors around mitotic chromosomes. Importin-ß and RanGTP have been implicated in additional cellular processes, but the precise roles of the Ran/importin-ß pathway throughout the cell cycle remain poorly understood. We implemented a FRET-based, high-throughput small molecule screen for compounds that interfere with the interaction between RanGTP and importin-ß and identified importazole, a 2,4-diaminoquinazoline. Importazole specifically blocks importin-ß-mediated nuclear import both in Xenopus egg extracts and cultured cells, without disrupting transportin-mediated nuclear import or CRM1-mediated nuclear export. When added during mitosis, importazole impairs the release of an importin-ß cargo FRET probe and causes both predicted and novel defects in spindle assembly. Together, these results indicate that importazole specifically inhibits the function of importin-ß, likely by altering its interaction with RanGTP. Importazole is a valuable tool to evaluate the function of the importin-ß/RanGTP pathway at specific stages during the cell cycle.

Plasmon-assisted transparency in metal-dielectric microspheres.

We present a theoretical analysis of light scattering from a layered metal-dielectric microsphere. The system consists of two spherical resonators coupled through concentric embedding. Solving for the modes of this system, we find that near an avoided crossing the scattering cross section is dramatically suppressed, exhibiting a tunable optical transparency. Similar to electromagnetically induced transparency, this phenomenon is associated with a large group delay, which in our system is manifest as flat azimuthal dispersion.

Enhanced surface-plasmon resonance absorption in metal-dielectric-metal layered microspheres.

We present a theoretical study of the dispersion relation of surface-plasmon resonances of mesoscopic metal-dielectric-metal microspheres. By analyzing the solutions to Maxwell's equations, we obtain a simple geometric condition for which the system exhibits a band of surface-plasmon modes whose resonant frequencies are weakly dependent on the multipole number. Using a modified Mie calculation, we find that a large number of modes belonging to this flat-dispersion band can be excited simultaneously by a plane wave, thus enhancing the absorption cross section. We demonstrate that the enhanced absorption peak of the sphere is geometrically tunable over the entire visible range.