ABSTRACT
OBJECTIVE: The aim of this study was to investigate the effects of the freeze drying and solvent dehydration allograft preservation methods on the mechanical properties of the graft material. METHODS: Bone blocks obtained from bovine humeri were divided into three groups. Samples in the first group were freeze dried, samples in the second group were dehydrated by solvents and samples in the third group were used as controls. All samples underwent three point bending test to investigate bending strength and were evaluated for calcium content. RESULTS: Bending strength of both the freeze-dried and solvent-dehydrated samples were found to have decreased significantly compared to that of the control group. The calcium content of the solvent dehydrated group was also significantly lower than that of the control group. CONCLUSION: Our results suggested that both preservation techniques, used to dehydrate bone, have negative effects on the bending strength of bone as they decrease the mechanical properties of the graft material.
Subject(s)
Bone Transplantation/methods , Calcium/metabolism , Desiccation/methods , Freeze Drying/methods , Preservation, Biological/methods , Animals , Cattle , Femur/surgery , Male , Models, Animal , Random Allocation , Risk Factors , Sensitivity and Specificity , Solvents/chemistry , Stress, Mechanical , Tensile Strength , Transplantation, HomologousABSTRACT
Polarographic anodic oxidation wave that can be correlated to total antioxidant capacity (TAC) in Human serum corresponds to 2H+ and 2e process. The limiting current of the wave, which is proportional to TAC, is strongly influenced by UV light intensity and irradiation time. A mechanism has been proposed to explain the effects of UV on anodic wave related to antioxidant behavior. Results show that decomposition processes follow to the excitation a transitory product at three different light intensities at 340 nm. The number of decomposition products increase with increasing light intensity. The rate of oxygen radical capture ability of the serum antioxidants might be estimated by comparing the changes of anodic wave in the absence and presence of naturally dissolved oxygen.
Subject(s)
Polarography , Serum/chemistry , Serum/radiation effects , Ultraviolet Rays , Antioxidants/chemistry , Electrodes , Humans , Kinetics , Oxidation-Reduction/radiation effects , Oxygen/chemistry , Time FactorsABSTRACT
There is a growing demand in Turkey for human tissue to use in surgery and wound healing. However, our country does not have facilities for local production of tissue grafts and generally depends on imported products. Under a multi-year project initiated in 1997, the International Atomic Energy Agency has provided main equipment for tissue processing and experts on Tissue Banking as well as training on tissue processing methods.In this presentation, information on various stages of the project implementation is given. Details of lay out for the process laboratories and equipment are given. Donor selection and testing criteria, processing procedures for bone and amnion, setting up product design, implementation of quality system and radiation sterilisation are described briefly. Quality procedures included preparation of quality manual, record forms, document control, non-conformance and corrective actions, training records, equipment maintenance and calibration are all in line with GMP/GLP Standards. Clinical applications of tissue grafts and medico-legal position of organ and tissue donation in Turkey are also discussed briefly.