ABSTRACT
Accumulating evidence has shown that transforming acidic coiled-coil 3 (TACC3) is deregulated in a broad spectrum of cancers. In the present study, we reported that TACC3 was markedly elevated in bladder cancer, especially in muscle-invasive bladder cancers (MIBCs). The upregulation of TACC3 was positively associated with tumor invasiveness, grade, T stage, and progression in patients with bladder cancer. Furthermore, a Kaplan-Meier survival analysis showed that patients with bladder cancer whose tumors had high TACC3 expression experienced a dismal prognosis compared with patients whose tumors had low TACC3 expression. Functional studies have found that TACC3 is a prerequisite for the development of malignant characteristics of bladder cancer cells, including cell proliferation and invasion. Moreover, TACC3 promoted G1/S transition, which was mediated via activation of the transcription of E2F1, eventually enhancing cell proliferation. Notably, the overexpression of TACC3 or E2F1 indicates a high sensitivity to cisplatin. Taken together, these findings define a tumor-supportive role for TACC3, which may also serve as a prognostic and therapeutic indicator in bladder cancers.
Subject(s)
Cisplatin/pharmacology , E2F1 Transcription Factor/genetics , Microtubule-Associated Proteins/metabolism , Transcription, Genetic/drug effects , Up-Regulation/genetics , Urinary Bladder Neoplasms/genetics , Urinary Bladder Neoplasms/pathology , Adult , Aged , Aged, 80 and over , Cell Line, Tumor , Cell Proliferation/drug effects , E2F1 Transcription Factor/metabolism , Female , G1 Phase/drug effects , Gene Expression Regulation, Neoplastic/drug effects , Humans , Male , Middle Aged , Neoplasm Invasiveness , Phenotype , Prognosis , S Phase/drug effects , Up-Regulation/drug effects , Young AdultABSTRACT
Astatotilapia burtoni is a species of fish in the Cichlidae family. Astatotilapia burtoni has been used as a model organism to research the behaviors and physical systems of cichlids. Here, we reported the complete mitogenome sequence of A. burtoni, which was 16,583 bp and composed of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region, with a base composition of lower G + C content (45.5%). Similar to Astatotilapia calliptera, all genes were located on H-strand except for eight tRNAs and ND6 genes. Most protein-coding genes started with an ATG codon except for COX1, ATP6 and ND3, which initiated with GTG or ATC instead, and terminated with the typical stop condon (TAA/TAG)or a single T. In this article, 12 protein-coding genes of other 10 closely species were used to construct the species phylogenetic tree to convince the mitogenome sequences. These results provided basic information for researching A. burtoni on genetics, phylogeny and adaptive evolution.
