Virulence and genetic analysis of Puccinia graminis tritici in the Indian sub-continent from 2016 to 2022 and evaluation of wheat varieties for stem rust resistance.

Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), has re-emerged as one of the major concerns for global wheat production since the evolution of Ug99 and other virulent pathotypes of Pgt from East Africa, Europe, Central Asia, and other regions. Host resistance is the most effective, economic, and eco-friendly approach for managing stem rust. Understanding the virulence nature, genetic diversity, origin, distribution, and evolutionary pattern of Pgt pathotypes over time and space is a prerequisite for effectively managing newly emerging Pgt isolates through host resistance. In the present study, we monitored the occurrence of stem rust of wheat in India and neighboring countries from 2016 to 2022, collected 620 single-pustule isolates of Pgt from six states of India and Nepal, analyzed them on Indian stem rust differentials, and determined their virulence phenotypes and molecular genotypes. The Ug99 type of pathotypes did not occur in India. Pathotypes 11 and 40A were most predominant during these years. Virulence phenotyping of these isolates identified 14 Pgt pathotypes, which were genotyped using 37 Puccinia spp.-specific polymorphic microsatellites, followed by additional phylogenetic analyses using DARwin. These analyses identified three major molecular groups, demonstrating fewer lineages, clonality, and long-distance migration of Pgt isolates in India. Fourteen of the 40 recently released Indian wheat varieties exhibited complete resistance to all 23 Pgt pathotypes at the seedling stage. Twelve Sr genes were postulated in 39 varieties based on their seedling response to Pgt pathotypes. The values of slow rusting parameters i.e. coefficient of infection, area under disease progress curve, and infection rates, assessed at adult plant stage at five geographically different locations during two crop seasons, indicated the slow rusting behavior of several varieties. Six Sr genes (Sr2, Sr57, Sr58, Sr24, Sr31, and Sr38) were identified in 24 wheat varieties using molecular markers closely linked to these genes. These findings will guide future breeding programs toward more effective management of wheat stem rust.

Imaging in international sporting event: experience from the Birmingham Commonwealth Games 2022.

AIM: To explain the design and delivery of diagnostic imaging and image-guided intervention services for an international games. The authors share their experiences from the Birmingham Commonwealth Games 2022. MATERIALS AND METHODS: A retrospective analysis was undertaken of anonymised data from the Zillion, Easyvision (RIS and PACS), and Encounter platforms for image viewing, interpretation and reporting during the Games. The data collected included age and gender, type of sport, nature of the injury, and imaging findings with diagnoses. RESULTS: The number of individuals who had radiological investigations at the Birmingham Commonwealth Games was 518 and the vast majority of them were athletes (90 %). The average age of athletes who had imaging was 28 years and that of non-athletes who accessed imaging services was 46.4 years with male predominance. Magnetic resonance imaging was the most frequently used imaging technique and the lower limb was the most frequently imaged body part. Athletes playing netball and beach volleyball had the highest percentage of injuries. CONCLUSION: The authors share their experience from the Birmingham Commonwealth Games 2022 regarding the nuances and challenges in radiology service provision for an international sports event that would be helpful for musculoskeletal radiologists in the design and delivery of similar international events in the future.

A Radiologists' Guide to En Bloc Resection of Primary Tumors in the Spine: What Does the Surgeon Want to Know?

En bloc resection in the spine is performed for both primary and metastatic bone lesions and has been proven to lengthen disease-free survival and decrease the likelihood of local recurrence. It is a complex procedure, which requires a thorough multi-disciplinary approach. This article will discuss the role of the radiologist in characterizing the underlying tumor pathology, staging the tumor and helping to predict possible intraoperative challenges for en bloc resection of primary bone lesions. The postoperative appearances and complications following en bloc resection in the spine will be considered in subsequent articles.

MRI imaging of soft tissues tumours and tumour like lesions-SLAM approach.

Imaging is vital in characterising and delineating the extent of soft tissue tumours and there is abundant literature on this. A simplified approach is required to characterise the lesions on MR and we describe a simplified street-smart approach called SLAM (signal, location, age, multiplicity and matrix).

Iliotibial band enthesopathy: an unusual cause of lateral knee pain post total knee replacement.

Iliotibial band (ITB) pathology is one of the main causes of lateral knee pain. The enthesopathy of the ITB at its insertion post total knee replacement (TKR) is a rare cause of lateral knee pain. We describe a series of cases of ITB enthesopathy with sonographic findings and management.

"Do-not-touch" lesions of bone revisited.

Incidental bone lesions are encountered frequently in day-to-day practice. Many of these lesions are indeterminate requiring referral to specialist centres for further characterisation with or without biopsy; however, as biopsy has its own drawbacks, not all lesions can be subjected to biopsy. The primary role of a radiologist in these situations is to characterise these lesions based on their imaging appearances into aggressive lesions requiring biopsy and non-aggressive lesions that do not require a biopsy. The term "do-not-touch lesion" is used to describe a lesion with typical radiographic appearances that can be characterised based on radiographic appearances alone without needing a biopsy. With recent advances in imaging, many incidental lesions can be characterised into do-not-touch lesions based on their imaging appearances alone using a single imaging technique or using a combination of imaging techniques and, less frequently, with the additional help of serological investigations, without the need for biopsy. Hence, the definition of do-not-touch lesions of bone needs a revisit. In this article, we attempt to redefine do-not-touch lesions of bone and propose an imaging-based classification for characterisation of these lesions.

A guide to assessing post-operative complications following en bloc spinal resection.

En-bloc resection of spinal tumours is a complex procedure with significant morbidity and mortality. The extensive resection leaves a large soft tissue and osseous defect requiring reconstruction. Following en-bloc resection, there may be complications relating to both the removal of the tumour and the subsequent reconstruction. This paper outlines the imaging appearances of the frequently encountered complications in our experience. The primary aim is to improve the confidence of the radiologist when reporting imaging following spinal en-bloc resection, however we believe this is also useful for the spinal and orthopaedic surgeons in assessing the patients following en block resection.

TI VIBE inversion MRI - An alternative to CT for imaging of hip pain.

INTRODUCTION: MR and CT are excellent complimentary diagnostic modalities for evaluation of hip pain which are often used together along with radiographs. However, CT involves radiation, which is a concern particularly in younger patients. T1VIBE is a 3D gradient echo MR sequence with high intrinsic contrast between the bone and soft tissues with multiplanar capabilities. AIM: We performed a study to assess if TIVIBE can be used to complement MR for evaluation of hip pain in young adults and to see if measurements and angles can be calculated using T1VIBE inversion images with similar accuracy to CT scan. MATERIAL AND METHODS: A retrospective review of 50 patients aged less than 40 years, who had MR (including TIVIBE) and CT of pelvis was performed. Post surgical patients were excluded. Some important measurements such as Centre edge angle, Tonnis' angle, anterior acetabular sector angle, posterior acetabular sector angle and acetabular version were independently measured by two readers on T1 VIBE inversion and CT images separately and measurements were compared. One reader performed the measurements again to assess for intra-observer error. RESULTS: There was a female predominance (37 F, 13M) with an average age of 27.6 years (range of 17-39). There was no significant difference in the measurements between CT and TI VIBE inversion and there was good intra and interobservor reliability. CONCLUSION: TI VIBE inversion sequence can be used as an alternative to CT with added advantage of alleviating the radiation exposure.

Spinal collision lesions.

Collision lesions are rare neoplasms often described in the hepatobiliary system, genitourinary system and adrenal glands. Vertebral haemangiomas (VH) are the most common lesions involving the vertebral bodies. VHs are usually asymptomatic and considered as "Do not touch" lesions. Rarely they can be symptomatic. Imaging findings of typical and atypical haemangiomas, variant forms of haemangioma such as aggressive haemangiomas are well known. Collision lesions involving VHs are extremely rare. This article presents a series of cases with collision lesions of the vertebral body involving VHs. ADVANCES IN KNOWLEDGE: This Case series demonstrates the various collision lesions in spinal haemangioma.

Resource utilization and health care charges associated with the most frequent ED users.

STUDY OBJECTIVE: Emergency department (ED) visits have continued to rise, and frequent ED users account for up to 8% of all ED visits. Reducing visits by frequent ED users may be one way to help reduce health care costs. We hypothesize that frequent users have unique ED utilization patterns resulting in differences in health care charges. METHODS: We conducted a retrospective review of electronic medical records from an urban community teaching hospital for the year 2012 comparing the top 108 frequent ED users (>12 visits/year) to a randomly selected group of 108 nonfrequent users (<4 visits/year). We compared demographic characteristics, distance lived from the hospital, medical and psychiatric history, substance abuse history, diagnostic testing, disposition, and amount charged to the patient for each visit. We compared data using χ(2) for proportions and t test or Wilcoxon rank sum based on normality of the data. RESULTS: The top 108 frequent ED users accounted for 1922 visits (2.9%), whereas the 108 nonfrequent users accounted for 150 visits (0.2%), in 2012 (all ED visits n = 65,398). Frequent users were more often unemployed, have public insurance, have mental health conditions, use tobacco, have a greater number of allergies to medications, and live closer to the hospital (P < .01). Disposition and median charge per visit did not differ between frequent and nonfrequent users ($1220 vs $1280). The total charges of the frequent ED users' visits were $10,465,216.07 versus $1,012,610.21 for nonfrequent users. CONCLUSIONS: Frequent users have unique medical and social characteristics; however, disposition and visit charges did not differ from nonfrequent users.

CO2 sensing at room temperature using carbon nanotubes coated core fiber Bragg grating.

The sensing of carbon dioxide (CO2) at room temperature, which has potential applications in environmental monitoring, healthcare, mining, biotechnology, food industry, etc., is a challenge for the scientific community due to the relative inertness of CO2. Here, we propose a novel gas sensor based on clad-etched Fiber Bragg Grating (FBG) with polyallylamine-amino-carbon nanotube coated on the surface of the core for detecting the concentrations of CO2 gas at room temperature, in ppm levels over a wide range (1000 ppm-4000 ppm). The limit of detection observed in polyallylamine-amino-carbon nanotube coated core-FBG has been found to be about 75 ppm. In this approach, when CO2 gas molecules interact with the polyallylamine-amino-carbon nanotube coated FBG, the effective refractive index of the fiber core changes, resulting in a shift in Bragg wavelength. The experimental data show a linear response of Bragg wavelength shift for increase in concentration of CO2 gas. Besides being reproducible and repeatable, the technique is fast, compact, and highly sensitive.

Ethnomedicinal plants used to treat bone fracture from North-Central Western Ghats of India.

ETHNOPHARMACOLOGICAL RELEVANCE: North Central Western Ghats in India comprises rich bio-cultural diversity and is also home to varied ethnomedicinal practices. The study was taken up for documentation and analysis of traditional knowledge regarding the practice and use of plants in the treatment of bone fracture. This is an effort to preserve the vanishing wealth of traditional knowledge. MATERIALS AND METHODS: Key informants identified in a preliminary survey and collection of information was through semi structured, open ended interviews. The details on age, place of practice, experience of key informants and learning of practice, disease they treat and mode of diagnosis, storage and usage of plants were collected. The identity of plants and their information was confirmed through repeated guided transect walks in different seasons with the informants and focus group discussions. Identified plants were deposited at the herbaria of Regional Medical Research Centre, Belgaum. RESULTS: Forty four key informants providing treatment for bone fracture in this region were identified. Thirty eight plant species belonging to 24 families have been documented in the present study. Highest number of species representation is found in families Euphorbiaceae and Fabaceae. The habit of the species showed that 45% of the herbal drugs were obtained from trees, followed by herbs, shrubs and climbers and majority of plants used were collected from wild (76%). The stem or stem bark (33%) was most commonly used plant part to prepare medicine. Twenty six formulations of 30 plant species were directly used in treating bone fracture, where Cissus quadrangularis has the highest use-value (0.14). Eleven plants were found to be administered for bone strengthening, pain relieving, inflammation reduction and speedy recovery and Gmelina arborea has the highest use value (0.27). CONCLUSIONS: The results indicated the importance of traditional herbal practices in community for their health needs. Both conservational strategies and further validation studies are the need of the hour for better utilization and sustenance of the documented knowledge.

Comparison of thermophilic and mesophilic one-stage, batch, high-solids anaerobic digestion.

The concept of starting up a batch, high-solids anaerobic digester by simply flooding the bed with a pH-buffer solution was tested using a mixture of vegetable waste and wood chips as feedstock at mesophilic (38 degrees C) and thermophilic (55 degrees C) conditions. At both temperatures stable and balanced methanogenesis was rapidly established within four days and was sustained until substrate was exhausted. Methanogenesis was more rapidly initiated in the thermophilic digester than in the mesophilic digester. Acetic, propionic and butyric acids accumulated in the leachate of both digesters during the start-up of digestion of uninoculated batch of waste. Thereafter all acids were degraded; which was quicker in the thermophilic digester. The accumulation and degradation of these acids was slower in the mesophilic digester. These studies showed that inoculum for carrying out thermophilic and mesophilic anaerobic digestion is readily available within the waste and its activity for complete mineralization of organic matter can be enhanced and sustained by providing adequate alkalinity. By employing a process in which anaerobic digestion of subsequent batches of waste was carried out by flooding with leachate drained from the digestion of a previous batch of waste, the volatile organic acid accumulation was maintained low and 95% of the methane yield potential of the waste was produced in 11 days under thermophilic conditions as opposed to 27 days under mesophilic conditions.

Herbal care for reproductive health: ethno medicobotany from Uttara Kannada district in Karnataka, India.

Traditional herbal medicine is predominantly practiced by the rural people of India, especially remote areas such as the Uttara Kannada District in Western Ghats of Karnataka. Local traditional healers play an important role in the management of reproductive health problems of the native population due to socio-economical and geographical factors. In the present study, 92 traditional medicine practitioners/healers from various regions of Uttara Kannada district were interviewed to collect information on the use of herbal treatments for a range of female and male reproductive disorders. Information was also collected on the method of preparation, dose and duration along with the botanical names, family and local names of the medicinal plants. The plants were then collected and identified. A total of 18 formulations from 25 plant species belonging to 17 families were identified, which are commonly used to treat 12 different reproductive ailments. This study identifies herbal remedies not previously documented, that are used by indigenous people in the treatment of reproductive disorders. Additionally, the paper highlights the need to retain and explore the rich biodiversity associated with Indian rain forests that may result in the discovery of new medical treatments. Finally, this paper notes the continuing reliance on herbal medicines and healing traditions by local people in remote areas. Understanding and working with local healers and tribes provides a unique opportunity to learn about the use of potentially new herbal and plant medications.

Ethnomedicine of Dharwad district in Karnataka, India--plants used in oral health care.

The present ethnomedicine survey covers the Dharwad district of Karnataka in southern India. It was revealed that 35 plants belonging to 26 families are being used to treat different types of oral ailments like toothache, plaque and caries, pyorrhea and aphthae. Sixteen of these plants were new claims for the treatment of oral ailments not previously reported in the ethnomedicinal literature of India. Basella alba, Blepharis repens, Capparis sepiaria, Oxalis corniculata and Ricinus communis are used for the treatment of aphthae; Azima tetracantha, Caesalpinia coriaria, Cleome gynandra, Gossypium herbacium, Leucas aspera, Merremia chryseides, Pergularia daemia, Prosopis juliflora and Solanum nigrum are used to treat tooth ache and Cassia hirsuta and Cassia tora are used in the treatment of plaque and caries.

Clinical profile of carcinoma stomach at a tertiary care hospital in south India.

Carcinoma of the stomach is an important cause of mortality due to cancer. Carcinoma of the stomach is common in the southern region of India. We conducted a retrospective study on the epidemiological, clinical and survival patterns among the patients with carcinoma of the stomach, attending our hospital from June 19, 1995 to 1st January 2003. All the patients had histopathological confirmation of malignancy. Patients with gastrooesophageal junction lesions were excluded. Surgery was performed with curative as well as palliative intent in suitable patients. Chemotherapy has been incorporated in to the combined modality treatment in our hospital since July 2000. Postoperative chemotherapy comprised commonly used intravenous chemotherapy regimens, while oral chemotherapy (etoposide) was given to patients with disease not amenable to surgery, and those having poor performance and nutritional status. Oral etoposide was given in a dose of 50 mg/day for 14 days, in a 28 day cycle. Quality of life was assessed in the oral chemotherapy group. Out of the 1749 cancer patients seen during the period, 151 had gastric malignancy (8.6%). The median age was 55 years (range 15-84 years). The male to female ratio was 4:1. Adenocarcinoma was found in 148 patients, 2 had stromal tumours and 1 had non-Hodgkin lymphoma. Stage disribution was as follows; stage 2-1 patient , stage 3a-25, stage3b-49, stage4-3 1, Metastatic-28. Staging was not completed in 17 patients. Eighty-nine patients underwent surgery. Fifty-nine patients (39%) did not have surgery. One patient underwent polypectomy. Curative gastrectomy was performed in 11 patients. Thirty-nine patients underwent palliative tumour resection. Palliative gastro-jejunostomy for relief of symptoms was performed in 26 patients and exploratory laparotomy alone was perforaied in 13. Thirty-eight patients received chemotherapy. Out of these, only 2 patients had prior complete resection of the tumour and 36 received palliative chemotherapy. Intravenous chemotherapy was given to 17 patients and oral chemotherapy to 19; All the patients who received oral etoposide did not experience any toxicity. Patients who received intravenous chemotherapy (n=17) had the following toxicities: grade 3 emesis in 4 (20%), discoloration of the skin and nails in 6(31%), alopecia in 8 (50%), grade 3 diarrhoea in 3 (15%) and neutropenic fever in 4 patients (20%). Median survival for the cohort was 10.4 months. Quality of life parameters, such as sleep, appetite, weight, pain, work and general sense of ill health showed improvement. In conclusion, 8.6% of all cancers at our hospital were due to cancer of stomach, in whom distal gastric tumor were more frequent and most were non-resectable. Median survival was 10.4 months. Oral etoposide was found to be safe, improved the quality of life and may play a role in the palliative management of advanced carcinoma of the stomach.

Ethnomedicobotany of Uttara Kannada District in Karnataka, India--plants in treatment of skin diseases.

Present paper deals with the herbal remedies for skin diseases in Uttara Kannada district of Karnataka--a Southern State in India, which is located in the hearts of Western Ghats. In an ethnobotanical exploration of this area 52 herbal preparations from 31 plants belonging to 21 families have been recorded. This also includes 17 new claims to the ethnomedical knowledge. The parts used and methods of preparation are discussed along with the family and local name for all the plants.

Ethnomedical knowledge of plants used by Kunabi Tribe of Karnataka in India.

Therapeutic effects and medicinal efficacy of the wild herbs were identified and administered by the tribal people to cure various ailments. Recently, the practice of herbal medicine has been diminishing, which may lead to the loss of valuable information about healing herbs. The Uttara Kannada District of Karnataka in India is one of the rich biodiversity centers of Western Ghats. Many tribes like Gowlis, Siddis, Halakki Okkaligas and Kunabis inhabit the semi-evergreen forests of the District. The mixed marathi speaking Kunabis have migrated from Goa and settled here a long time back and are even today without modern facilities. Thus, in the way of revitalization of traditional herbal medicines, it is an effort to record the valuable ethnomedical knowledge of the tribe Kunabis of Uttara Kannada District in Karnataka. A total of 45 species of plants used by Kunabi community people are described here based on an ethnomedical field survey. These plants belong to 26 families and are used to treat a wide range of discomforts like fever, cough, skin diseases, rheumatism, snakebite, jaundice, dysentery, etc.

Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsii.

In order to investigate the functional roles of a phytopathogenic fungal lectin (SRL) isolated from the bodies of Sclerotium rolfsii, the binding properties of SRL were studied by enzyme linked lectinosorbent assay and by inhibition of SRL-glycan interaction. Among glycoproteins (gp) tested for binding, SRL reacted strongly with GalNAc alpha1-->4Ser/Thr (Tn) and/or Gal beta1-->3GalNAc alpha1-->(T(alpha)) containing gps: human T(alpha) and Tn glycophorin, asialo salivary gps, and asialofetuin, but its reactivity toward sialylated glycoproteins was reduced significantly. Of the sugar ligands tested for inhibition of SRL-asialofetuin binding, Thomsen-Friedenreich residue (T(alpha)) was the best, being 22.4 and 2.24 x 10(3) more active than GalNAc and Gal beta1--> residues, respectively. Other ligands tested were inactive. When the glycans used as inhibitors, T(alpha), and/or Tn containing gps, especially asialo PSM, asialo BSM, asialo OSM, active antifreeze gp, asialo glycophorin and Tn-glycophorin were very active, and 1.0 x 10(4) times more potent than GalNAc. From these results, it is clear that the combining site of SRL should be of a cavity type and recognizes only Tn and T(alpha) residues of glycans; it is suggested that T(alpha) and Tn glycotopes, which are present only in abnormal carbohydrate sequences of higher orders of mammal, are the most likely sites for phytopathogenic fungal attachment as an initial step of infection. The affinity of SRL for ligands can be ranked in decreasing order as follows: multivalent T(alpha) and Tn >> monomeric T(alpha) and Tn > GalNAc >>> II (Gal beta1-->4GlcNAc), L (Gal beta1-->4Glc), and Gal.