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1.
Nat Commun ; 14(1): 1033, 2023 02 23.
Article in English | MEDLINE | ID: mdl-36823144

ABSTRACT

The malaria parasite Plasmodium falciparum causes substantial human mortality, primarily in equatorial Africa. Enriched in affected African populations, the B*53 variant of HLA-B, a cell surface protein that presents peptide antigens to cytotoxic lymphocytes, confers protection against severe malaria. Gorilla, chimpanzee, and bonobo are humans' closest living relatives. These African apes have HLA-B orthologs and are infected by parasites in the same subgenus (Laverania) as P. falciparum, but the consequences of these infections are unclear. Laverania parasites infect bonobos (Pan paniscus) at only one (TL2) of many sites sampled across their range. TL2 spans the Lomami River and has genetically divergent subpopulations of bonobos on each side. Papa-B, the bonobo ortholog of HLA-B, includes variants having a B*53-like (B07) peptide-binding supertype profile. Here we show that B07 Papa-B occur at high frequency in TL2 bonobos and that malaria appears to have independently selected for different B07 alleles in the two subpopulations.


Subject(s)
Histocompatibility Antigens Class I , Malaria, Falciparum , Pan paniscus , Plasmodium , Animals , Malaria, Falciparum/genetics , Pan paniscus/genetics , Pan paniscus/parasitology , Peptides , Phylogeny , Histocompatibility Antigens Class I/genetics
2.
J Hered ; 110(3): 261-274, 2019 05 07.
Article in English | MEDLINE | ID: mdl-31067326

ABSTRACT

The outbreak and transmission of disease-causing pathogens are contributing to the unprecedented rate of biodiversity decline. Recent advances in genomics have coalesced into powerful tools to monitor, detect, and reconstruct the role of pathogens impacting wildlife populations. Wildlife researchers are thus uniquely positioned to merge ecological and evolutionary studies with genomic technologies to exploit unprecedented "Big Data" tools in disease research; however, many researchers lack the training and expertise required to use these computationally intensive methodologies. To address this disparity, the inaugural "Genomics of Disease in Wildlife" workshop assembled early to mid-career professionals with expertise across scientific disciplines (e.g., genomics, wildlife biology, veterinary sciences, and conservation management) for training in the application of genomic tools to wildlife disease research. A horizon scanning-like exercise, an activity to identify forthcoming trends and challenges, performed by the workshop participants identified and discussed 5 themes considered to be the most pressing to the application of genomics in wildlife disease research: 1) "Improving communication," 2) "Methodological and analytical advancements," 3) "Translation into practice," 4) "Integrating landscape ecology and genomics," and 5) "Emerging new questions." Wide-ranging solutions from the horizon scan were international in scope, itemized both deficiencies and strengths in wildlife genomic initiatives, promoted the use of genomic technologies to unite wildlife and human disease research, and advocated best practices for optimal use of genomic tools in wildlife disease projects. The results offer a glimpse of the potential revolution in human and wildlife disease research possible through multi-disciplinary collaborations at local, regional, and global scales.


Subject(s)
Animal Diseases/etiology , Animals, Wild , Genomics , Research , Animal Diseases/epidemiology , Animal Diseases/transmission , Animals , Biodiversity , Biological Evolution , Computational Biology/methods , Disease Susceptibility , Ecology , Environment , Genome , Genomics/methods , Host-Pathogen Interactions/genetics , Humans
3.
Food Chem Toxicol ; 47(2): 353-60, 2009 Feb.
Article in English | MEDLINE | ID: mdl-19068223

ABSTRACT

Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are effector molecules of RNA interference (RNAi), a highly conserved RNA-based gene suppression mechanism in plants, mammals and other eukaryotes. Endogenous RNAi-based gene suppression has been harnessed naturally and through conventional breeding to achieve desired plant phenotypes. The present study demonstrates that endogenous small RNAs, such as siRNAs and miRNAs, are abundant in soybean seeds, corn kernels, and rice grain, plant tissues that are traditionally used for food and feed. Numerous endogenous plant small RNAs were found to have perfect complementarity to human genes as well as those of other mammals. The abundance of endogenous small RNA molecules in grain from safely consumed food and feed crops such as soybean, corn, and rice and the homology of a number of these dietary small RNAs to human and animal genomes and transcriptomes establishes a history of safe consumption for dietary small RNAs.


Subject(s)
DNA, Plant/genetics , Edible Grain/genetics , MicroRNAs/genetics , RNA, Small Interfering/genetics , Sequence Homology, Nucleic Acid , Animals , Consumer Product Safety , DNA, Plant/analysis , Edible Grain/chemistry , Genome , Humans , MicroRNAs/analysis , RNA, Small Interfering/analysis , Seeds/chemistry , Sequence Alignment , Glycine max/chemistry , Glycine max/genetics
4.
PLoS One ; 3(8): e2871, 2008 Aug 06.
Article in English | MEDLINE | ID: mdl-18716673

ABSTRACT

Small RNAs (approximately 20 to 24 nucleotides) function as naturally occurring molecules critical in developmental pathways in plants and animals. Here we analyze small RNA populations from mature rice grain and seedlings by pyrosequencing. Using a clustering algorithm to locate regions producing small RNAs, we classified hotspots of small RNA generation within the genome. Hotspots here are defined as 1 kb regions within which small RNAs are significantly overproduced relative to the rest of the genome. Hotspots were identified to facilitate characterization of different categories of small RNA regulatory elements. Included in the hotspots, we found known members of 23 miRNA families representing 92 genes, one trans acting siRNA (ta-siRNA) gene, novel siRNA-generating coding genes and phased siRNA generating genes. Interestingly, over 20% of the small RNA population in grain came from a single foldback structure, which generated eight phased 21-nt siRNAs. This is reminiscent of a newly arising miRNA derived from duplication of progenitor genes. Our results provide data identifying distinct populations of small RNAs, including phased small RNAs, in mature grain to facilitate characterization of small regulatory RNA expression in monocot species.


Subject(s)
Oryza/genetics , RNA, Plant/isolation & purification , Seeds/genetics , Animals , Base Sequence , Conserved Sequence , Humans , MicroRNAs/genetics , RNA, Messenger/genetics , RNA, Plant/classification , RNA, Plant/genetics , Species Specificity
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