ABSTRACT
Proteins belonging to the enhancer of RNA interference-1 subfamily of 3'-5' exoribonucleases participate in divergent RNA pathways. They degrade small interfering RNAs (siRNAs), thus suppressing RNA interference, and are involved in the maturation of ribosomal RNAs and the degradation of histone messenger RNAs (mRNAs). Here, we report evidence for the role of the plant homologue of these proteins, which we termed ENHANCED RNA INTERFERENCE-1-LIKE-1 (ERIL1), in chloroplast function. In vitro assays with AtERIL1 proved that the conserved 3'-5' exonuclease activity is shared among all homologues studied. Confocal microscopy revealed that ERL1, a nucleus-encoded protein, is targeted to the chloroplast. To gain insight into its role in plants, we used Nicotiana benthamiana and Arabidopsis thaliana plants that constitutively overexpress or suppress ERIL1. In the mutant lines of both species we observed malfunctions in photosynthetic ability. Molecular analysis showed that ERIL1 participates in the processing of chloroplastic ribosomal RNAs (rRNAs). Lastly, our results suggest that the missexpression of ERIL1 may have an indirect effect on the microRNA (miRNA) pathway. Altogether our data point to an additional piece of the puzzle in the complex RNA metabolism of chloroplasts.
Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , RNA, Ribosomal/metabolism , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Chloroplasts/metabolism , Exoribonucleases/genetics , Exoribonucleases/metabolism , Gene Expression Regulation, Plant , RNA Interference , RNA, Ribosomal/genetics , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Nicotiana/genetics , Nicotiana/metabolismABSTRACT
Agroinfiltration is a very fast and powerful method to express in planta any sequences in a transient fashion. Agroinfiltration has proven very useful for the overexpression of proteins in the infiltrated zone when a short-term effect can be informative. However, it has been a real success story in the induction of local and eventually systemic silencing. Here, we describe the use of agroinfiltration for the induction of local silencing of an endogene or a transgene, for the systemic silencing of a transgene and for co-infiltration assays. We also provide protocols for the evaluation of the efficiency of the assay, by detecting the specific siRNAs characteristic of RNA silencing and measuring the effects on the target sequences.
Subject(s)
Gene Transfer Techniques , RNA Interference , Down-Regulation/genetics , Gene Expression Regulation, Plant/genetics , Genes, Plant/genetics , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Plant Leaves/genetics , Plant Leaves/metabolism , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Nicotiana/genetics , Nicotiana/metabolismABSTRACT
Higher eukaryotes have developed a mechanism of sequence-specific RNA degradation which is known as RNA silencing. In plants and some animals, similar to the nematode Caenorhabditis elegans, RNA silencing is a non-cell-autonomous event. Hence, silencing initiation in one or a few cells leads progressively to the sequence-specific suppression of homologous sequences in neighbouring cells in an RNA-mediated fashion. Spreading of silencing in plants occurs through plasmodesmata and results from a cell-to-cell movement of a short-range silencing signal, most probably 21-nt siRNAs (short interfering RNAs) that are produced by one of the plant Dicer enzymes. In addition, silencing spreads systemically through the phloem system of the plants, which also translocates metabolites from source to sink tissues. Unlike the short-range silencing signal, there is little known about the mediators of systemic silencing. Recent studies have revealed various and sometimes surprising genetic elements of the short-range silencing spread pathway, elucidating several aspects of the processes involved. In this review we attempt to clarify commonalities and differences between the individual silencing pathways of RNA silencing spread in plants.
Subject(s)
Plants , RNA Interference , Animals , Gene Expression Regulation, Plant , MicroRNAs/genetics , MicroRNAs/metabolism , Plants/genetics , Plants/metabolism , Plants, Genetically Modified , RNA, Plant , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , TransgenesABSTRACT
Myeloperoxidase (MPO), eosinophil peroxidase, lactoperoxidase, and thyroid peroxidase are heme-containing oxidoreductases (EC 1.7.1.11), which bind ligands and/or undergo a series of redox reactions. Though sharing functional and structural homology, reflecting their phylogenetic origin, differences are observed regarding their spectral features, substrate specificities, redox properties, and kinetics of interconversion of the relevant redox intermediates ferric and ferrous peroxidase, compound I, compound II, and compound III. Depending on substrate availability, these heme enzymes path through the halogenation cycle and/or the peroxidase cycle and/or act as poor (pseudo-)catalases. Based on the published crystal structures of free MPO and its complexes with cyanide, bromide and thiocyanate as well as on sequence analysis and modeling, we critically discuss structure-function relationships. This analysis highlights similarities and distinguishing features within the mammalian peroxidases and intents to provide the molecular and enzymatic basis to understand the prominent role of these heme enzymes in host defense against infection, hormone biosynthesis, and pathogenesis.