ABSTRACT
Genetic studies on human personality have provided little satisfactory results to date mainly because of the complexity of this trait. Neonatal temperament using observational measures is an alternative phenotype to approach genetics to human behavior. An association study was conducted on 117 Caucasian newborns. Their temperament was evaluated using the Neonatal Behavior Assessment Scale 48 h after birth. Thirteen polymorphisms in the SLC6A4, DRD4 and TFAP2B genes were genotyped. Linear regression was performed to analyze data, and Bonferroni correction was applied. To check the functional effect of the TFAP2B Indel Intron 2 polymorphism, reporter gene luciferase assays using a mouse cortical neural progenitor cell line and quantitative polymerase chain reaction (qPCR) studies in human post-mortem brain samples were performed. A significant association was found between 5-HTTLPR, 5-HTTLPR + rs25531 and TFAP2B Indel Intron 2 with Range of State cluster as well as an interaction between rs25531 and TFAP2B Indel Intron 2 with Range of State. DRD4 variable number tandem repeat exon 3 was associated with orientation. A 30% increase in the luciferase levels of the TFAP2B 5-repeat alleles compared with the 6-repeat alleles (P-value = 0.03) was found using the pGL3 promoter vector. The qPCR experiments showed the same trend as the in vitro studies, although no significant results were obtained. This study supports a role of the SLC6A4, DRD4 and TFAP2B genes in the temperament, including a gene-gene interaction between SLC6A4 and TFAP2B. It also provides evidence about an effect of the TFAP2B polymorphism in TFAP2B gene transcription.
Subject(s)
Infant Behavior/physiology , Receptors, Dopamine D4/genetics , Serotonin Plasma Membrane Transport Proteins/genetics , Temperament/physiology , Transcription Factor AP-2/genetics , Alleles , Female , Genetic Association Studies , Genotype , Humans , Infant, Newborn , Male , Minisatellite Repeats , Polymorphism, GeneticSubject(s)
Membrane Glycoproteins/genetics , Membrane Transport Proteins/genetics , Nerve Tissue Proteins/genetics , Polymorphism, Genetic , Receptor, Serotonin, 5-HT1A/genetics , Amygdala/physiology , Female , Genotype , Humans , Neurons/physiology , Reference Values , Serotonin Plasma Membrane Transport Proteins , Statistics, NonparametricABSTRACT
The purpose of this study was to determine the effectiveness of the Supplemental Food Program for Women, Infants, and Children (WIC) for mothers and their anemic children under 5 years of age. Sample for the study was 50 anemic children, 25 in each of two groups. The interventions (treatments) for the experimental group included individual counseling, group nutrition classes, and provision of WIC food vouchers for purchasing foods containing essential nutrients that are deficient in the diets of this high-risk population. An initial dietary assessment was done, and hemoglobin and hematocrit measurements were taken. At the end of 6 months, each participant's diet was reassessed, and hemoglobin and hematocrit measurements were again taken. Results of the study showed a 1.8 gm change in the hemoglobin measurements of the members of the experimental group and a 1.1 gm change in the hemoglobin measurements of the members of the control group.
Subject(s)
Anemia/diet therapy , Food Services , Child, Preschool , Dietary Services , Female , Health Promotion , Hematocrit , Hemoglobins , Humans , Infant , Male , Nutritional Requirements , Outcome and Process Assessment, Health CareABSTRACT
This study shows that alloantigen presentation via the anterior chamber (AC) of the eye coupled with a single high dose cyclophosphamide (CP) (100 mg/kg) treatment effectively suppresses the skin graft rejection reaction of the recipient. Lewis (Le) rats bearing allogeneic Brown Norway (BN) skin implants in the AC of the eye demonstrate a modest increase in the survival time of orthotopic BN skin grafts. A slight prolongation of the survival of orthotopic BN skin grafts was also demonstrated in nonimplant or syngeneic implant-bearing Le recipients which received a single injection of a large dose of CP. Augmentation of suppression was evident in rats which were treated with a single dose of 75 mg/kg CP but not 25 mg/kg. The augmentation of suppression was evident when CP treatment and skin grafting of the recipient occurred on either 0, 7, or 14 days postimplantation. Recipient splenectomy did not interfere with the augmentation of suppression.