OSIRIS-REx Contamination Control Strategy and Implementation.

OSIRIS-REx will return pristine samples of carbonaceous asteroid Bennu. This article describes how pristine was defined based on expectations of Bennu and on a realistic understanding of what is achievable with a constrained schedule and budget, and how that definition flowed to requirements and implementation. To return a pristine sample, the OSIRIS-REx spacecraft sampling hardware was maintained at level 100 A/2 and <180 ng/cm2 of amino acids and hydrazine on the sampler head through precision cleaning, control of materials, and vigilance. Contamination is further characterized via witness material exposed to the spacecraft assembly and testing environment as well as in space. This characterization provided knowledge of the expected background and will be used in conjunction with archived spacecraft components for comparison with the samples when they are delivered to Earth for analysis. Most of all, the cleanliness of the OSIRIS-REx spacecraft was achieved through communication among scientists, engineers, managers, and technicians.

The fall, recovery, orbit, and composition of the Tagish Lake meteorite: a new type of carbonaceous chondrite.

The preatmospheric mass of the Tagish Lake meteoroid was about 200,000 kilograms. Its calculated orbit indicates affinity to the Apollo asteroids with a semimajor axis in the middle of the asteroid belt, consistent with a linkage to low-albedo C, D, and P type asteroids. The mineralogy, oxygen isotope, and bulk chemical composition of recovered samples of the Tagish Lake meteorite are intermediate between CM and CI meteorites. These data suggest that the Tagish Lake meteorite may be one of the most primitive solar system materials yet studied.

Measurement of radiographic changes in adjuvant-induced arthritis in rats by quantitative image analysis.

OBJECTIVE: To apply quantitative analytical methods to the evaluation of radiographic images in experimental arthritis. METHODS: Adjuvant was used to induce arthritis in rats. Arthritis progression was followed by conventional methods. In addition, digitized images of radiographs of the calcaneus were examined for changes in the mean and in the distribution pattern of gray values. Periosteal new bone formation was measured as an increase in image area of the calcaneus. RESULTS: Significant changes in the gray value profile and increases in periosteal bone formation occurred in arthritic rats. More extensive changes occurred in Lewis rats than in Sprague-Dawley rats. Analysis of serial radiographs revealed an initial decrease in the density of juxtaarticular bone, followed by progressive increases in gray value variation due to concurrent bone loss and bone formation. Eventually, bone formation in arthritic rats resulted in increased gray values above those in nonarthritic rats. CONCLUSION: Image analysis represents a sensitive, quantitative method for detecting radiographic changes in experimental arthritis.

Proximal impact deposits at the Cretaceous-Tertiary boundary in the Gulf of Mexico: a restudy of DSDP Leg 77 Sites 536 and 540.

Restudy of Deep Sea Drilling Project Sites 536 and 540 in the southeast Gulf of Mexico gives evidence for a giant wave at Cretaceous-Tertiary boundary time. Five units are recognized: (1) Cenomanian limestone underlies a hiatus in which the five highest Cretaceous stages are missing, possibly because of catastrophic K-T erosion. (2) Pebbly mudstone, 45 m thick, represents a submarine landslide possibly of K-T age. (3) Current-bedded sandstone, more than 2.5 m thick, contains anomalous iridium, tektite glass, and shocked quartz; it is interpreted as ejecta from a nearby impact crater, reworked on the deep-sea floor by the resulting tsunami. (4) A 50-cm interval of calcareous mudstone containing small Cretaceous planktic foraminifera and the Ir peak is interpreted as the silt-size fraction of the Cretaceous material suspended by the impact-generated wave. (5) Calcareous mudstone with basal Tertiary forams and the uppermost tail of the Ir anomaly overlies the disturbed interval, dating the impact and wave event as K-T boundary age. Like Beloc in Haiti and Mimbral in Mexico, Sites 536 and 540 are consistent with a large K-T age impact at the nearby Chicxulub crater.

Tektite-bearing, deep-water clastic unit at the Cretaceous-Tertiary boundary in northeastern Mexico.

The hypothesis of Cretaceous-Tertiary (K-T) boundary impact on Yucatán, Mexico, predicts that nearby sites should show evidence of proximal impact ejecta and disturbance by giant waves. An outcrop along the Arroyo el Mimbral in northeastern Mexico contains a layered clastic unit up to 3 m thick that interrupts a biostratigraphically complete pelagic-marl sequence deposited at more than 400 m water depth. The marls were found to be unsuitable for determining magnetostratigraphy, but foraminiferal biostratigraphy places the clastic unit precisely at the K-T boundary. We interpret this clastic unit as the deposit of a megawave or tsunami produced by an extraterrestrial impact. The clastic unit comprises three main subunits. (1) The basal "spherule bed" contains glass in the form of tektites and microtektites, glass spherules replaced by chlorite-smectite and calcite, and quartz grains showing probable shock features. This bed is interpreted as a channelized deposit of proximal ejecta. (2) A set of lenticular, massive, graded "laminated beds" contains intraclasts and abundant plant debris, and may be the result of megawave backwash that carried coarse debris from shallow parts of the continental margin into deeper water. (3) At the top, several thin "ripple beds" composed of fine sand are separated by clay drapes; they are interpreted as deposits of oscillating currents, perhaps a seiche. An iridium anomaly (921 +/- 23 pg/g) is observed at the top of the ripple beds. Our observations at the Mimbral locality support the hypothesis of a K-T impact on nearby Yucatán.

Proximal cretaceous-tertiary boundary impact deposits in the Caribbean.

Trace element, isotopic, and mineralogic studies indicate that the proposed impact at the Cretaceous-Tertiary (K-T) boundary occurred in an ocean basin, although a minor component of continental material is required. The size and abundance of shocked minerals and the restricted geographic occurrence of the ejecta layer and impact-wave deposits suggest an impact between the Americas. Coarse boundary sediments at sites 151 and 153 in the Colombian Basin and 5- to 450-meter-thick boundary sediments in Cuba may be deposits of a giant wave produced by a nearby oceanic impact. On the southern peninsula of Haiti, a approximately 50-centimeter-thick ejecta layer occurs at the K-T boundary. This ejecta layer is approximately 25 times as thick as that at any known K-T site and suggests an impact site within approximately 1000 kilometers. Seismic reflection profiles suggest that a buried approximately 300-km-diameter candidate structure occurs in the Colombian Basin.

Bioassay of teicoplanin in serum containing rifampin or a beta-lactam antibiotic.

The purpose of this study was to develop bioassays for the measurement of teicoplanin in serum containing rifampin or a beta-lactam antibiotic. Use of rifampin-resistant Bacillus subtilis as indicator organism or pretreatment of the serum sample with Bacillus cereus penicillinase Type I (nafcillin, ticarcillin, mezlocillin) or Type II (cefazolin, cefuroxime, ceftazidime, ceftriaxone) effectively eliminated assay interference. Validation bioassays performed on two separate days utilizing triplicate coded serum samples containing 0 to 200 micrograms teicoplanin in combination with 40 micrograms/ml rifampin or 200 to 500 micrograms/ml beta-lactam showed no significant differences (p greater than 0.05, two-way analysis of variance) in analyte recovery between assay days. Regression analysis of each teicoplanin/rifampin or teicoplanin/beta-lactam data set yielded slope values of 0.92 to 1.01, intercept values of -0.45 to 0.84 and correlation coefficients of 0.9925 to 0.9990. Thus, serum teicoplanin can be quantitated accurately, precisely, and reproducibly in patients receiving concomitant rifampin or beta-lactam chemotherapy.

A sensitive bioassay for teicoplanin in serum in the presence or absence of other antibiotics.

A teicoplanin bioassay has been developed that is accurate, sensitive, and reliable. A linear relationship is obtained between the diameter of the zone of inhibition and log10 teicoplanin concentration in human serum over the range of 0.15 or 1.25 to 96 micrograms/ml using wells or paper filter disks, respectively. The assay medium devised consists of 50 g BBL Mueller-Hinton II Agar, 30 g NaCl, 8 g CaCl2, and 1.0 g citric acid (monohydrate) per liter of deionized water (resulting pH 5.1 +/- 0.1) and the assay organism Bacillus subtilis ATCC 6633. This system allows the assay of teicoplanin in the presence of commonly used aminoglycosides and in the presence of beta-lactams after inactivation by beta-lactamase. Additionally, it has the potential to be used in the presence of rifampin by using a rifampin-resistant strain of B. subtilis.