ABSTRACT
It has been previously demonstrated that hybrid Ty virus-like particles (VLP) prime effective CD8(+) and CD4(+) T cell responses. In this study, we investigated the effect of treating mice with Ty VLP carrying the immunodominant epitope of Der p 1 after sensitizing them to the group 1 allergen of the house dust mite Dermatophagoides pteronyssinus (Der p 1), under conditions that induce T(h)2 immunity. We show that i.p. treatment with the hybrid VLP abrogated allergen-specific IL-5 production and reduced allergen-specific cell proliferation. This suppression of the response was mediated by CD4(+) T cells and was not accompanied by an increase in IFN-gamma production.
Subject(s)
Allergens/immunology , CD4-Positive T-Lymphocytes/physiology , Epitopes, T-Lymphocyte , Glycoproteins/immunology , Immunodominant Epitopes/immunology , Interferon-gamma/biosynthesis , Interleukin-5/biosynthesis , Animals , Antigens, Dermatophagoides , Cells, Cultured , Down-Regulation , Female , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Mites/immunology , Peptide Fragments/pharmacologySubject(s)
Lymphogranuloma Venereum/surgery , Perineum/surgery , Skin Transplantation , Adult , Female , Humans , Transplantation, AutologousABSTRACT
Partially hepatectomized mice were injected with inducers of interferon, and the mitotic activity of liver cells was measured. The inducers were polyriboinosinic.polyribocytidylic acid, poly(I.C), Newcastle disease virus, and statolon. Each inhibited the mitosis of liver cells. Poly(I.C) was effective in doses as low as 1 mug per mouse. Polyriboinosinic acid, poly(I), had no inhibitory effect. These results extend the spectrum of action of inducers of interferon to inhibition of mitotic division of an aneoplastic, nonaneuploid mammalian cell.