ABSTRACT
We evaluated the influence of hesperidin and vitamin C (VitC) on glycemic parameters, lipid profile, and DNA damage in male Wistar rats treated with sucrose overload. Rats were divided into six experimental groups: I-water control; II-sucrose control; III-hesperidin control; IV-VitC control; V-co-treatment of sucrose plus hesperidin; VI-co-treatment of sucrose plus VitC. We measured the levels of triglycerides, total cholesterol, HDL-c, LDL-c, fasting glucose, and glycated hemoglobin (A1C). DNA damage was evaluated in blood and brain cells using the comet assay and the micronucleus test was used to evaluate chromosomal damages in the rat bone marrow. Co-treatment with VitC, but not with hesperidin, normalized the serum glucose. No effect of co-treatments was observed on A1C. The co-treatment with VitC or hesperidin did not influence the lipid profile (p>0.05). Rats co-treated with hesperidin had a significantly lower DNA damage level in blood (p<0.05) and brain (p<0.05). Rats treated with VitC only, but not those co-treated with VitC plus sucrose, had significantly higher DNA damage in brain (p<0.05). No significant differences were observed in the results of micronucleus test (p>0.05). Hesperidin and VitC showed different effects on sucrose and DNA damage levels. While VitC lowered the serum glucose, hesperidin reduced the DNA damage.
Subject(s)
Ascorbic Acid/pharmacology , Blood Glucose/analysis , DNA Damage , Glycated Hemoglobin/analysis , Hesperidin/pharmacology , Lipids/blood , Sucrose/administration & dosage , Vitamins/pharmacology , Animals , Blood Glucose/drug effects , Fasting/blood , Glycated Hemoglobin/drug effects , Male , Micronucleus Tests , Rats , Rats, Wistar , Sucrose/bloodABSTRACT
The purpose of this study was to determine the effects of the high consumption of sucrose on the levels of DNA damage in blood, hippocampus and bone marrow of rats. Male Wistar rats were treated for 4 months with sucrose (10% for 60 initial days and 34% for the following 60 days) in drinking water, and then, glycemia and glycated hemoglobin (A1C) were measured. Levels of DNA damage in blood and hippocampus were evaluated by the comet assay. The micronucleus test was used to evaluate chromosomal damages in the bone marrow. The sucrose treatment significantly increased (p<0.01) the serum glucose levels (~20%) and A1C (~60%). The level of primary DNA damage was significantly increased (p<0.05) in hippocampal cells (~60%) but not in peripheral blood leukocytes (p>0.05). Additionally, it was observed a significative increase (p<0.05) in the markers of chromosomal breaks/losses in bone marrow, as indicated by the micronucleus test. This is the first study that evaluated DNA damage induced by high sucrose concentration in the hippocampus and bone marrow of rats. Sucrose-induced DNA damage was observed in both tissues. However, the mechanism of sucrose toxicity on DNA remains unknown.
Subject(s)
Bone Marrow/drug effects , DNA Damage , Dietary Sucrose/adverse effects , Hippocampus/drug effects , Animals , Bone Marrow/pathology , Comet Assay , Diabetes Mellitus, Type 2/complications , Disease Models, Animal , Hippocampus/pathology , Male , Micronucleus Tests , Rats , Rats, WistarABSTRACT
ABSTRACT The purpose of this study was to determine the effects of the high consumption of sucrose on the levels of DNA damage in blood, hippocampus and bone marrow of rats. Male Wistar rats were treated for 4 months with sucrose (10% for 60 initial days and 34% for the following 60 days) in drinking water, and then, glycemia and glycated hemoglobin (A1C) were measured. Levels of DNA damage in blood and hippocampus were evaluated by the comet assay. The micronucleus test was used to evaluate chromosomal damages in the bone marrow. The sucrose treatment significantly increased (p<0.01) the serum glucose levels (~20%) and A1C (~60%). The level of primary DNA damage was significantly increased (p<0.05) in hippocampal cells (~60%) but not in peripheral blood leukocytes (p>0.05). Additionally, it was observed a significative increase (p<0.05) in the markers of chromosomal breaks/losses in bone marrow, as indicated by the micronucleus test. This is the first study that evaluated DNA damage induced by high sucrose concentration in the hippocampus and bone marrow of rats. Sucrose-induced DNA damage was observed in both tissues. However, the mechanism of sucrose toxicity on DNA remains unknown.
Subject(s)
Animals , Male , Rats , Bone Marrow/drug effects , DNA Damage , Hippocampus/drug effects , Bone Marrow/pathology , Micronucleus Tests , Rats, Wistar , Dietary Sucrose/adverse effects , Comet Assay , Diabetes Mellitus, Type 2/complications , Disease Models, Animal , Hippocampus/pathologyABSTRACT
Abstract Objective: To evaluate the possible association between hyperuricemia and cardiorespiratory fitness levels/nutritional profile, grouped into a single variable, in schoolchildren. Method: Cross-sectional study of 2335 students from Elementary schools, aged 7-17 years of both genders, stratified by conglomerates of a municipality in Southern Brazil. Body mass index (BMI) was calculated and cardiorespiratory fitness (CRF) was assessed by the 6-minute run/walk test. The BMI and CRF were grouped into a single variable, considering: (1) low and normal weight/fit; (2) low and normal weight/unfit; (3) overweight-obesity/fit; (4) overweight-obesity/unfit. The Poisson regression (prevalence ratio, PR) was used for the association between hyperuricemia and BMI/CRF ratio with 95% confidence intervals and differences were considered significant when p < 0.05. Results: There is an association, although subtle, between the presence of hyperuricemia with low levels of CRF and the presence of excess weight, when grouped into a single variable. Boys and girls with this condition have higher prevalence of hyperuricemia (PR: 1.07; p = 0.007 for boys; PR: 1.10; p < 0.001 for girls). Conclusion: Together, excess weight and low levels of cardiorespiratory fitness are associated with the presence of hyperuricemia in schoolchildren.
Resumo Objetivo: Avaliar a possível relação entre hiperuricemia com aptidão cardiorrespiratória e o estado nutricional, agrupados, em escolares. Método: Estudo transversal com 2.335 escolares da educação básica de 7 a 17 anos, de ambos os sexos, estratificados por conglomerados de um munícipio do sul do Brasil. Foi calculado o índice de massa corporal (IMC) e a aptidão cardiorrespiratória (APCR) foi avaliada pelo teste de corrida/caminhada de 6 minutos. O IMC e a APCR foram agrupados em uma única variável, considerando: 1) baixo peso-normal/apto; 2) baixo peso-normal/inapto; 3) sobrepeso-obesidade/apto; 4) sobrepeso-obesidade/inapto. A regressão de Poisson (razão de prevalência; RP) foi usada para associação entre hiperuricemia e a relação APCR/IMC com intervalos de confiança de 95% e diferenças significativas consideradas para p < 0,05. Resultados: Observa-se associação, embora sutil, entre a presença de hiperuricemia com baixos níveis de APCR e a presença de excesso de peso, de forma agrupada. Meninos e meninas, com essa condição, têm maior prevalência de hiperuricemia (RP: 1,07; p = 0,007; RP: 1,10; p < 0,001, respectivamente), em comparação com seus pares com bons níveis de APCR e estado nutricional adequado. Conclusão: De forma conjunta, o excesso de peso e os baixos níveis de aptidão cardiorrespiratória estão associados com a presença de hiperuricemia em escolares.
Subject(s)
Humans , Male , Female , Child , Adolescent , Hyperuricemia/physiopathology , Cardiorespiratory Fitness/physiology , Obesity/physiopathology , Nutritional Status , Cross-Sectional Studies , Exercise TestABSTRACT
OBJECTIVE: To evaluate the possible association between hyperuricemia and cardiorespiratory fitness levels/nutritional profile, grouped into a single variable, in schoolchildren. METHOD: Cross-sectional study of 2335 students from Elementary schools, aged 7-17 years of both genders, stratified by conglomerates of a municipality in Southern Brazil. Body mass index (BMI) was calculated and cardiorespiratory fitness (CRF) was assessed by the 6-minute run/walk test. The BMI and CRF were grouped into a single variable, considering: (1) low and normal weight/fit; (2) low and normal weight/unfit; (3) overweight-obesity/fit; (4) overweight-obesity/unfit. The Poisson regression (prevalence ratio, PR) was used for the association between hyperuricemia and BMI/CRF ratio with 95% confidence intervals and differences were considered significant when p<0.05. RESULTS: There is an association, although subtle, between the presence of hyperuricemia with low levels of CRF and the presence of excess weight, when grouped into a single variable. Boys and girls with this condition have higher prevalence of hyperuricemia (PR: 1.07; p=0.007 for boys; PR: 1.10; p<0.001 for girls). CONCLUSION: Together, excess weight and low levels of cardiorespiratory fitness are associated with the presence of hyperuricemia in schoolchildren.
Subject(s)
Cardiorespiratory Fitness/physiology , Hyperuricemia/physiopathology , Obesity/physiopathology , Adolescent , Child , Cross-Sectional Studies , Exercise Test , Female , Humans , Male , Nutritional StatusABSTRACT
The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD.
Subject(s)
Cardiovascular Diseases/etiology , DNA Damage , Glucose/analysis , Lipids/blood , Adolescent , Biomarkers/blood , Cardiovascular Diseases/blood , Cardiovascular Diseases/genetics , Child , Comet Assay , Female , Humans , Male , Prevalence , Risk FactorsABSTRACT
The purpose of the present study was to evaluate the identification of 19 Brazilian C. dubliniensis based on the biochemical profile exhibited when tested by the commercial identification kit ID 32C (bioMerieux). Thirteen of the isolates were rigorously identified as C. dubliniensis and the remaining isolates (six) were considered as having a doubtful profile but the software also suggested that there was 83.6% of chances for them to be C. dubliniensis. As well as pointed by the literature the identification obtained by phenotypic tests should be considered presumptive for C. dubliniensis due to variability of this new species.
Subject(s)
Candida/classification , Carbohydrate Metabolism , Mycological Typing Techniques/methods , Candida/isolation & purification , Candida/metabolism , Humans , PhenotypeABSTRACT
Dezenove culturas de C. dubliniensis isoladas no Brasil, previamente identificadas através de métodos genotípicos, foram avaliadas pelo kit comercial ID 32C (bioMerieux). Treze culturas foram identificadas como C. dubliniensis, mas as demais (seis) evidenciaram perfil duvidoso, embora o software do sistema sugerisse 83,6% de chances das mesmas pertencerem à espécie C. dubliniensis. A literatura tem registrado grande variabilidade fenotípica com esta espécie e, por isto, as identificações obtidas com este sistema deverão ser consideradas como presuntivas.
Subject(s)
Humans , Candida/classification , Carbohydrates/metabolism , Mycological Typing Techniques/methods , Candida/isolation & purification , Candida/metabolism , PhenotypeABSTRACT
Este estudo foi realizado com os primeiros isolados ambientais de C. neoformans sorotipo D, obtidos no Rio Grande do Sul. Objetivando-se avaliar a susceptibilidade a agentes antifúngicos de forma mais rigorosa, utilizou-se a técnica de referência proposta pelo NCCLS, Caldo Yeast Nitrogen Base (YNB) proposto por Ghannoum et al., Antibiotic medium 3, caldo YNB adequado à metodologia do NCCLS e o E-test. Os resultados indicaram que todos os isolados foram sensíveis à anfotericina B (0,0625-0,5 µg/mL), fluconazol (0,125-4,0 µg/mL), itraconazol (0,031-0,25 µg/ml) e fluorocitosina (0,125-4,0 µg/mL) através das técnicas empregadas. Nos testes de termotolerância (47ºC/30 min), observou-se que as culturas de C. neoformans sorotipo D são mais sensíveis do que as de C. neoformans sorotipo A.