Assessment of the relationship between plasma fibrinogen-to-albumin ratio and slow coronary flow phenomenon in patients without obstructive coronary artery disease.

BACKGROUND: Prior studies have suggested that the chronic inflammatory response has an important role in the pathophysiology of slow coronary flow phenomenon (SCFP). However, data are scarce regarding the role of plasma fibrinogen-to-albumin ratio (PFAR) in patients having SCFP without obstructive coronary artery disease (CAD). In this study, we investigated the relationship between PFAR and the presence of SCFP in patients without obstructive CAD. METHODS: From January 2021 to January 2023, we consecutively recruited 1085 patients without obstructive CAD according to the diagnostic and exclusion criteria. In total, SCFP was diagnosed in 70 patients. A 1:2 age-matched case-control study was then conducted using comparators without SCFP. Ultimately, this study enrolled 70 patients with angiographically normal coronary arteries and SCFP, along with 140 comparators with angiographically normal coronary arteries and normal coronary flow. Plasma fibrinogen and albumin levels were measured, and the PFAR was then calculated for each patient. RESULTS: PFARs were significantly greater in the SCFP group than in the comparators with normal coronary flow (82.8 ± 15.4 vs 73.1 ± 19.5, p < 0.001). PFAR increased with increasing numbers of vessels affected by SCFP. Multivariate logistic regression analysis showed that PFAR was an independent predictor of SCFP (odds ratio: 1.818, p = 0.015). Receiver operating characteristic (ROC) curve analysis indicated that PFAR showed a better predictive value of SCFP than fibrinogen or albumin, although not significantly (p > 0.05). CONCLUSION: PFAR is an independent predictor of SCFP in patients without obstructive CAD. PAFR could improve the predictive value of SFCP than albumin or fibrinogen alone, but not significantly.

Construction, expression and functional analysis of anti-clenbuterol codon-optimized scFv recombinant antibody.

The construction, expression and functional analysis of codon-optimized single-chain variable fragment (coscFv) against clenbuterol (CBL) prepared from the Escherichia coli system is described. First, the ionic concentration for coscFv expression was optimized through single-factor experiments. Then, the extraction conditions of inclusion bodies were optimized, and coscFv was affinity-purified. Finally, the functional analysis of coscFv was elucidated by indirect competitive enzyme-linked immunosorbent assay (icELISA) and molecular docking. After optimizing the ionic concentration, the yield of coscFv increased from 21.69% to 23.26%. The molecular weight of coscFv was determined to be approximately 27 kDa according to the SDS-PAGE and Western blot assay. The percentage of coscFv was as high as 43.9% after the inclusion bodies were extracted, washed, and dissolved. Functional analysis indicated that the coscFv recognized CBL, and the 50% inhibition average concentration of CBL (IC50) was 4.22 ± 0.01 (n = 3) ng/mL. The binding site between coscFv and CBL consisted of Asp33H, Met34H, Ser50H, Arg52H, Tyr57H, Leu59H, Asp99H, and Tyr93L. Our study confirms that coscFv can bind with CBL through the key amino acid residues and can be used to sensitively detect CBL.

[An analysis of impact factor of Chinese Journal of Internal Medicine from 2008 to 2010].

OBJECTIVE: To analyze the articles and citation published in the Chinese Journal of Internal Medicine from 2008 to 2010, in order to investigate the influence factors of impact factor (IF). METHODS: All articles published in the Chinese Journal of Internal Medicine covered by Chinese Medical Citation Index(CMCI) from 2008 to 2010 were searched and downloaded. Some article related attributions were manual added and their influences to IF were analyzed. RESULTS: A total of 1164 academic papers were published in the journal in 3 years, with 9.95 references per paper. The total citation was 1029 times, with 0.93 time per paper and 0.31 time per page. Among them, 736 articles were not cited, accounting for 63.2%. Original articles, pure clinical articles had good citation output. For fund supporting, the citation of articles without fund was higher than those with fund. The articles on hematology, cardiology and gastroenterology accounted the most, while ICU, emergency and nephrology were the least internal medicine reported specialties. Although there exists citation difference among different subjects, for considering the absolute values, neurology/psychiatry (0.73 time per paper), cardiology (0.65 time per paper) and gastroenterology (0. 54 time per paper) had better citation output, while hematology, basic research and rheumatology had no good performance to IF. CONCLUSION: We should further strengthen acquisition and dissemination of excellent articles, reduce the number of non-cited paper, expand periodical visibility, and provide a quick and convenient way of literature reading.

[The citation analysis of Chinese Journal of Internal Medicine from 2005 to 2011].

OBJECTIVE: To evaluate the academic level of Chinese Journal of Internal Medicine by analyzing its citation status by using bibliometrics method. METHODS: The distribution of articles published in Chinese Journal of Internal Medicine from January, 2005 to December, 2011 indexed by Chinese Science Citation Database (CSCD) was analyzed. RESULTS: A total of 2809 articles were published in Chinese Journal of Internal Medicine between January,2005 and December, 2011. Among them, 832(29.62%) articles were cited for totally 1993 times. There were 14 authors whose total citation number in 2005-2011 was > or = 10 times. Authors of the cited articles came from 25 provinces, autonomous regions and municipalities. The regions from where the articles had the highest citation were Beijing (341 articles), Shanghai (87 articles), Guangdong (64 articles), Jiangsu (45 articles) and Zhejiang (43 articles). The medical institutions with the highest citation were Peking Union Medical College Hospital of Peking Union Medical College and Chinese Academy of Medical Sciences (205 times), Peking University People's Hospital (77 times), and Chinese PLA General Hospital (76 times). CONCLUSIONS: Quite a few high level academic papers had been published in Chinese Journal of Internal Medicine in recent years. Chinese Journal of Internal Medicine plays a good role in enhancing academic exchange.