ABSTRACT
In addition to their classical antigen presenting functions, MHC class II molecules potentiate the TLR-triggered production of pro-inflammatory cytokines. Here, we have addressed the effect of Tollip and MARCH1 on the regulation of MHC II trafficking and TLR signaling. Our results show that MARCH1-deficient mice splenocytes are impaired in their capacity to produce pro-inflammatory cytokines in response to poly(I:C) and that TLR3 and MHC II molecules interact in the endocytic pathway. Knocking down Tollip expression in human CIITA(+) HeLa cells increased expression of HLA-DR but reduced the proportion of MHC II molecules associated with the CLIP peptide. Truncation of the HLA-DR cytoplasmic tails abrogated the effect of Tollip on MHC class II expression. While overexpression of Tollip did not affect HLA-DR levels, it antagonized the function of co-transfected MARCH1. We found that Tollip strongly reduced MARCH1 protein levels and that the two molecules appear to compete for binding to MHC II molecules. Altogether, our results demonstrate that Tollip regulates MHC class II trafficking and that MARCH1 may represent a new Tollip target.
ABSTRACT
Group B Streptococcus (GBS) type III is an important agent of life-threatening invasive infections. Albeit the immune system plays a dual role in development and protection against disease, mechanisms leading to an efficient immune response against GBS remain obscure. Mouse bone marrow-derived dendritic cells (DCs) and primary spleen DCs were used to evaluate GBS capacity to modulate the functions of these important antigen-presenting cells. The role of capsular polysaccharide (CPS), one of the most important GBS virulence factors, in bacterial-DC interactions was evaluated by using a non-encapsulated mutant. Phagocytosis assays, confocal and electron microscopy showed that DCs efficiently internalize encapsulated GBS, but the latter possesses strong intracellular survival capacity. GBS devoid of CPS was internalized and killed at higher and faster rates than encapsulated GBS early after infection. Among several cytokines tested, GBS internalization was required for modulation of IL-12, IL-10 and CXCL10 pathways. In contrast, GBS induced DC expression of co-stimulatory molecules in a phagocytosis-independent manner. Finally, the production of pro-inflammatory and Th1 cytokines by GBS-stimulated DCs was differentially modulated by CPS expression, depending on DC origin. Our data suggest multiple mechanisms involved in GBS modulation of DC functions, which were selectively regulated by the presence of CPS.
Subject(s)
Bacterial Capsules/immunology , Dendritic Cells/immunology , Streptococcus agalactiae/immunology , Animals , Cytokines/metabolism , Mice , Microbial Viability , Microscopy, Confocal , Microscopy, Electron , Phagocytosis , Th1 Cells/immunologyABSTRACT
Group B Streptococcus (GBS) capsular type III is an important agent of life-threatening invasive infections. It has been previously shown that encapsulated GBS is easily internalized by dendritic cells (DCs) and can persist inside these immune cells. The mechanisms underlying these processes are unknown. Here, colocalization studies and the use of endocytosis inhibitors and caveolin(-/-) mice, demonstrated that GBS uses multiple endocytosis mechanisms to enter mouse DCs. The capsular polysaccharide (CPS) selectively drives GBS internalization via caveolae-independent but lipid raft-dependent pathways. Non-encapsulated bacteria failed to engage lipid rafts. GBS internalization by DCs also occurs via clathrin-mediated endocytosis in a process independent of bacterial CPS. Albeit caveolae are not required for GBS internalization, signalling events through caveolin-1 are involved in production of the inflammatory chemokine CCL2 by DCs infected with encapsulated GBS only. This study addresses for the first time endocytosis pathways implicated in DC internalization of encapsulated GBS and suggests a complex interplay between GBS and DCs, which was selectively modulated by the presence of CPS.
Subject(s)
Bacterial Capsules/immunology , Clathrin/metabolism , Dendritic Cells/microbiology , Dendritic Cells/physiology , Endocytosis , Membrane Microdomains/metabolism , Streptococcus agalactiae/pathogenicity , Animals , Bacterial Capsules/metabolism , Caveolin 1/metabolism , Chemokine CCL2/metabolism , Dendritic Cells/immunology , Mice , Mice, Knockout , Streptococcus agalactiae/immunologyABSTRACT
Streptococcus suis type 2 is a major swine pathogen and a zoonotic agent, causing meningitis in both swine and humans. S. suis infects the host through the respiratory route, reaches the bloodstream, and persists until breaching into the central nervous system. The capsular polysaccharide (CPS) of S. suis type 2 is considered a key virulence factor of the bacteria. Though CPS allows S. suis to adhere to the membrane of cells of the immune system, it provides protection against phagocytosis. In fact, nonencapsulated mutants are easily internalized and killed by macrophages and dendritic cells. The objective of this work was to study the molecular mechanisms by which the CPS of S. suis prevents phagocytosis. By using latex beads covalently linked with purified CPS, it was shown that CPS itself was sufficient to inhibit entry of both latex beads and bystander fluorescent beads into macrophages. Upon contact with macrophages, encapsulated S. suis was shown to destabilize lipid microdomains at the cell surface, to block nitric oxide (NO) production during infection, and to prevent lactosylceramide accumulation at the phagocytic cup during infection. In contrast, the nonencapsulated mutant was easily internalized via lipid rafts, in a filipin-sensitive manner, leading to lactosylceramide recruitment and strong NO production. This is the first report to identify a role for CPS in lipid microdomain stability and to recognize an interaction between S. suis and lactosylceramide in phagocytes.
Subject(s)
Antigens, CD/metabolism , Lactosylceramides/metabolism , Membrane Microdomains/drug effects , Phagocytosis/drug effects , Polysaccharides, Bacterial/metabolism , Polysaccharides, Bacterial/pharmacology , Streptococcus suis/metabolism , Animals , Anti-Bacterial Agents/pharmacology , Bacterial Adhesion , Carbohydrate Conformation , Cells, Cultured , Female , Filipin/pharmacology , Gene Expression Regulation, Bacterial/physiology , Macrophages/cytology , Macrophages/physiology , Mice , Microspheres , Polysaccharides, Bacterial/chemistry , Streptococcus suis/drug effects , Streptococcus suis/pathogenicity , VirulenceABSTRACT
Streptococcus suis is an emerging zoonotic agent of septicemia and meningitis. Knowledge on host immune responses toward S. suis and strategies used by this pathogen for subversion of these responses is scarce. Here, S. suis modulation of dendritic cell (DC) functions were assessed for the first time. Using S. suis knockout mutants in capsular polysaccharide (CPS) expression, it was shown that CPS blocks DC phagocytosis and impairs cytokine release by hindering cell wall components. Mutants impaired in D-alanylation of lipoteichoic acid (LTA) or N-deacetylation of peptidoglycan (PG) further demonstrated the importance of cell wall in modulation of DC activation. Notably, LTA/PG modifications were identified as major players in resistance to complement-dependent killing by DCs. Finally, S. suis hemolysin was partially involved in cytokine release and also contributed to bacterial escape of opsonophagocytosis. Overall, S. suis uses its arsenal of virulence factors to modulate DC functions and escape immune surveillance.
Subject(s)
Bacterial Capsules/metabolism , Bacteriolysis , Cell Wall/metabolism , Complement System Proteins/immunology , Dendritic Cells/immunology , Hemolysin Proteins/immunology , Streptococcus suis/immunology , Animals , Cell Wall/immunology , Cytokines/metabolism , Dendritic Cells/drug effects , Female , Hemolysin Proteins/metabolism , Immune Evasion , Lipopolysaccharides/immunology , Lipopolysaccharides/metabolism , Mice , Peptidoglycan/immunology , Peptidoglycan/metabolism , Phagocytosis , Streptococcus suis/chemistry , Teichoic Acids/immunology , Teichoic Acids/metabolismABSTRACT
The murine astrocyte response to virulent Streptococcus suis, a swine and an emerging human meningitis-causing pathogen, is reported. Albeit astrocytes do not internalize S. suis, all S. suis strains studied enhanced Toll-like receptor (TLR)2 expression and the production of pro-inflammatory cytokines and inducible nitric oxide synthase. Cell wall components and hemolysin (suilysin) are shown to be mainly responsible for cell activation. Astrocytes from TLR2 knockout mice presented a partial but significant reduction of S. suis-induced production of pro-inflammatory cytokines. These results contribute to increase the knowledge on mechanisms underlying S. suis inflammation in the brain.
Subject(s)
Astrocytes/metabolism , Astrocytes/microbiology , Gene Expression Regulation/physiology , Streptococcus suis , Toll-Like Receptor 2/metabolism , Analysis of Variance , Animals , Animals, Newborn , Cell Survival , Cells, Cultured , Cerebral Cortex/cytology , Cricetinae , Cytokines/metabolism , Disease Models, Animal , Gene Expression Regulation/genetics , Glial Fibrillary Acidic Protein/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Microscopy, Confocal , Mutation/genetics , Nitric Oxide Synthase Type II , Phagocytosis/physiology , RNA, Messenger/metabolism , Streptococcus suis/pathogenicity , Time Factors , Toll-Like Receptor 2/deficiencyABSTRACT
Streptococcus suis is an important swine and human pathogen responsible for septicemia and meningitis. In vivo research in mice suggested that in the brain, microglia might be involved in activating the inflammatory response against S. suis. The aim of this study was to better understand the interactions between S. suis and microglia. Murine microglial cells were infected with a virulent wild-type strain of S. suis. Two isogenic mutants deficient at either capsular polysaccharide (CPS) or hemolysin production were also included. CPS contributed to S. suis resistance to phagocytosis and regulated the inflammatory response by hiding proinflammatory components from the bacterial cell wall, while the absence of hemolysin, a potential cytotoxic factor, did not have a major impact on S. suis interactions with microglia. Wild-type S. suis induced enhanced expression of Toll-like receptor 2 by microglial cells, as well as phosphotyrosine, protein kinase C, and different mitogen-activated protein kinase signaling events. However, cells infected with the CPS-deficient mutant showed overall stronger and more sustained phosphorylation profiles. CPS also modulated inducible nitric oxide synthase expression and further nitric oxide production from S. suis-infected microglia. Finally, S. suis-induced NF-κB translocation was faster for cells stimulated with the CPS-deficient mutant, suggesting that bacterial cell wall components are potent inducers of NF-κB. These results contribute to increase the knowledge of mechanisms underlying S. suis inflammation in the brain and will be useful in designing more efficient anti-inflammatory strategies for meningitis.
Subject(s)
Communicable Diseases, Emerging/microbiology , Encephalitis/microbiology , Meningitis, Bacterial/microbiology , Microglia/microbiology , Streptococcal Infections/microbiology , Streptococcus suis/physiology , Zoonoses/microbiology , Animals , Cell Line , Chemokines/physiology , Cytokines/physiology , Encephalitis/physiopathology , Enzyme-Linked Immunosorbent Assay , Gene Expression Regulation, Bacterial/physiology , Host-Pathogen Interactions/physiology , Meningitis, Bacterial/physiopathology , Mice , Microglia/physiology , NF-kappa B/metabolism , Nitric Oxide/biosynthesis , Phagocytosis/physiology , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction/physiologyABSTRACT
IL-10 is a potent anti-inflammatory cytokine interfering with antigen presentation by inducing the intracellular sequestration of MHC class II (MHC-II) molecules. Here we studied the contribution of membrane-associated RING-CH (MARCH) ubiquitin ligase family members to the IL-10-induced down-regulation of MHC-II molecules. We found that MARCH1 and MARCH8 proteins are the most potent family members for the down-regulation of MHC-II surface expression in transfected cells, but only MARCH1 mRNA expression is strongly induced by IL-10 in human primary monocytes. We detected mono- and poly-ubiquitinated forms of MHC-II molecules both in IL-10-treated monocytes and in cells transfected with MARCH1. We also show direct interaction between MHC-II and MARCH1 molecules in co-immunoprecipitation assays. Finally, we found that siRNA-mediated knockdown of MARCH1 reverses IL-10-induced MHC-II down-regulation in primary monocytes. Thus, the immunosuppressive effect of IL-10 on antigen presentation is mediated through induced expression of MARCH1.
Subject(s)
HLA-D Antigens/metabolism , Interleukin-10/physiology , Monocytes/metabolism , Ubiquitin-Protein Ligases/physiology , B7-2 Antigen/metabolism , Down-Regulation , Gene Expression/drug effects , HLA-DR Antigens/metabolism , HeLa Cells , Humans , Interferon-gamma/pharmacology , Interleukin-10/pharmacology , Membrane Proteins/metabolism , Monocytes/drug effects , Nuclear Proteins/genetics , Protein Binding , RNA, Small Interfering/genetics , Trans-Activators/genetics , Ubiquitin-Protein Ligases/genetics , Ubiquitination/immunologyABSTRACT
Macrophages are immune cells that function in the clearance of infectious particles. This process involves the engulfment of microbes into phagosomes where these particles are lysed and degraded. In the current study, we used a large scale quantitative proteomics approach to analyze the changes in protein abundance induced on phagosomes by interferon-gamma (IFN-gamma), an inflammatory cytokine that activates macrophages. Our analysis identified 167 IFN-gamma-modulated proteins on phagosomes of which more than 90% were up-regulated. The list of phagosomal proteins regulated by IFN-gamma includes proteins expected to alter phagosome maturation, enhance microbe degradation, trigger the macrophage immune response, and promote antigen loading on major histocompatibility complex (MHC) class I molecules. A dynamic analysis of IFN-gamma-sensitive proteins by Western blot indicated that newly formed phagosomes display a delayed proteolytic activity coupled to an increased recruitment of the MHC class I peptide-loading complex. These phagosomal conditions may favor antigen presentation by MHC class I molecules on IFN-gamma-activated macrophages.
Subject(s)
Interferon-gamma/pharmacology , Macrophages/immunology , Phagosomes/immunology , Proteome/analysis , Proteomics/methods , Animals , Cell Line , Chromatography, Liquid , Cross-Priming/drug effects , Electrophoresis, Gel, Two-Dimensional , Histocompatibility Antigens Class I/immunology , Mass Spectrometry , Mice , Phagosomes/chemistry , Phagosomes/drug effectsABSTRACT
Tripeptidyl-peptidase II (TPPII) is a serine peptidase highly expressed in malignant Burkitt's lymphoma cells (BL). We have previously shown that overexpression of TPPII correlates with chromosomal instability, centrosomal and mitotic spindle abnormalities and resistance to apoptosis induced by spindle poisons. Furthermore, TPPII knockdown by RNAi was associated with endoreplication and the accumulation of polynucleated cells that failed to complete cell division, indicating a role of TPPII in the cell cycle. Here we have applied a global approach of gene expression analysis to gain insights on the mechanism by which TPPII regulates this phenotype. mRNA profiling of control and TPPII knockdown BL cells identified one hundred and eighty five differentially expressed genes. Functional categorization of these genes highlighted major physiological functions such as apoptosis, cell cycle progression, cytoskeleton remodeling, proteolysis, and signal transduction. Pathways and protein interactome analysis revealed a significant enrichment in components of MAP kinases signaling. These findings suggest that TPPII influences a wide network of signaling pathways that are regulated by MAPKs and exerts thereby a pleiotropic effect on biological processes associated with cell survival, proliferation and genomic instability.
ABSTRACT
CD8+ T cells are generated in response to Leishmania major (Lm) or Toxoplasma gondii parasitic infections, indicating that exogenously delivered Ag can be processed for presentation by MHC class I molecules. We show that presentation of Lm nucleotidase (NT)-OVA is TAP independent in vivo and in vitro, and is inhibited by chloroquine, but not by proteasome inhibitors. In contrast, the presentation of T. gondii P30-OVA relies on the TAP/proteasome pathway. Presentation of OVA- or rNT-OVA-coated beads also bypassed TAP requirement above a certain Ag threshold. TAP was also dispensable for the presentation of wild-type Lm Ags to primed CD8+ T cells in vitro. Finally, in vivo priming of CD8+ T cells involved in acquired resistance to Lm was not compromised in TAP-deficient mice. Thus, Leishmania Ags appear to be confined to an intraphagosomal processing pathway that requires higher concentrations of Ags, suggesting that these parasites may have evolved strategies to impair the efficient endoplasmic reticulum-based, TAP-dependent cross-presentation pathway to avoid or delay CD8+ T cell priming.
Subject(s)
ATP-Binding Cassette Transporters/metabolism , Antigen Presentation/immunology , Antigens, Protozoan/metabolism , CD8-Positive T-Lymphocytes/immunology , Leishmania major/immunology , Signal Transduction/immunology , ATP Binding Cassette Transporter, Subfamily B, Member 2 , ATP-Binding Cassette Transporters/genetics , ATP-Binding Cassette Transporters/physiology , Animals , Antigen Presentation/genetics , Antigens, Protozoan/genetics , CD8-Positive T-Lymphocytes/metabolism , Cells, Cultured , Coculture Techniques , Cross-Priming/immunology , Dendritic Cells/immunology , Dendritic Cells/metabolism , Egg Proteins/metabolism , Leishmania major/genetics , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Ovalbumin/metabolism , Peptide Fragments , Signal Transduction/genetics , Toxoplasma/immunologyABSTRACT
Phagocytosis, the process by which cells internalize large particulate materials from their milieu and sequester them in phagosomes, plays a role in a variety of cell functions ranging from nutrition in ameba to innate and adaptive immunity in mammals. Recent findings revealed unexpected characteristics of phagosomes, highlighting how this complex organelle may have evolved, from Dictyostelium to human, to become a key player in our ability to mount an efficient immune response against a variety of intracellular pathogens.
Subject(s)
Nutritional Physiological Phenomena , Phagocytosis/physiology , Animals , Antigen Presentation/immunology , Cross-Priming/immunology , Endoplasmic Reticulum/immunology , Endoplasmic Reticulum/metabolism , Humans , Phagosomes/immunology , Phagosomes/metabolismABSTRACT
Clearance of pathogens by phagocytosis and their killing in phagolysosomes is a key aspect of our innate ability to fight infectious agents. Leishmania parasites have evolved ways to survive and replicate in macrophages by inhibiting phagosome maturation and avoiding the harsh environment of phagolysosomes. We describe here that during this process Leishmania donovani uses a novel strategy involving its surface lipophosphoglycan (LPG), a virulence factor impeding many host functions, to prevent the formation or disrupt lipid microdomains on the phagosome membrane. LPG acts locally on the membrane and requires its repetitive carbohydrate moieties to alter the organization of microdomains. Targeting and disruption of functional foci, where proteins involved in key aspects of phagolysosome biogenesis assemble, is likely to confer a survival advantage to the parasite.
Subject(s)
Glycosphingolipids/metabolism , Leishmania donovani/metabolism , Macrophages/metabolism , Membrane Microdomains/metabolism , Phagosomes/metabolism , Animals , Cell Line , Intracellular Membranes/metabolism , Lipid Metabolism , Macrophages/parasitology , Macrophages/ultrastructure , Membrane Microdomains/ultrastructure , Mice , Phagosomes/ultrastructureABSTRACT
The ability to process microbial antigens and present them at the surface of cells is an important aspect of our innate ability to clear infections. It is generally accepted that antigens in the cytoplasm are loaded in the endoplasmic reticulum and presented at the cell surface on major histocompatibility complex (MHC) class I molecules, whereas peptides present in endo/phagocytic compartments are presented on MHC class II molecules. Despite the apparent segregation of the class I and class II pathways, antigens from intracellular pathogens including mycobacteria, Escherichia coli, Salmonella typhimurium, Brucella abortus and Leishmania, have been shown to elicit an MHC class-I-dependent CD8+ T-cell response, a process referred to as cross-presentation. The cellular mechanisms allowing the cross-presentation pathway are poorly understood. Here we show that phagosomes display the elements and properties needed to be self-sufficient for the cross-presentation of exogenous antigens, a newly ascribed function linked to phagocytosis mediated by the endoplasmic reticulum.
Subject(s)
Antigen Presentation , Antigens/immunology , Endoplasmic Reticulum/metabolism , Phagosomes/immunology , Phagosomes/metabolism , Amino Acid Sequence , Animals , Antigens/chemistry , Antigens/metabolism , CD8-Positive T-Lymphocytes/immunology , Cysteine Endopeptidases/metabolism , Histocompatibility Antigens Class I/immunology , Histocompatibility Antigens Class I/metabolism , Macrophages/cytology , Macrophages/immunology , Macrophages/metabolism , Mice , Multienzyme Complexes/metabolism , Ovalbumin/chemistry , Ovalbumin/immunology , Ovalbumin/metabolism , Phagocytosis , Proteasome Endopeptidase Complex , Ubiquitin/metabolismABSTRACT
The signaling pathways mediating human intestinal epithelial cell differentiation remain largely undefined. Phosphatidylinositol 3-kinase (PI3K) is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In this study, we analyzed the involvement of PI3K in the differentiation of human intestinal epithelial cells. We showed that inhibition of PI3K signaling in Caco-2/15 cells repressed sucrase-isomaltase and villin protein expression. Morphological differentiation of enterocyte-like features in Caco-2/15 cells such as epithelial cell polarity and brush-border formation were strongly attenuated by PI3K inhibition. Immunofluorescence and immunoprecipitation experiments revealed that PI3K was recruited to and activated by E-cadherin-mediated cell-cell contacts in confluent Caco-2/15 cells, and this activation appears to be essential for the integrity of adherens junctions and association with the cytoskeleton. We provide evidence that the assembly of calcium-dependent adherens junctions led to a rapid and remarkable increase in the state of activation of Akt and p38 MAPK pathways and that this increase was blocked in the presence of anti-E-cadherin antibodies and PI3K inhibitor. Therefore, our results indicate that PI3K promotes assembly of adherens junctions, which, in turn, control p38 MAPK activation and enterocyte differentiation.