Real-time monitoring abscisic acid release from single rice protoplast by amperometry at microelectrodes modified with abscisic acid receptor PYL2.

It was previously reported that stress induces a cellular production of abscisic acid in plants, but no direct method shows the evidence. Here, an electrochemical microsensor involving an abscisic acid receptor PYL2 modified carbon fiber microelectrode was fabricated by self-assembly method, where the Cu2+ combined with the histidine tag of PYL2 on the surface of microelectrode was used as the detection probe, the mediated reaction between Cu+ and ferricyanide realized the amplification responses and provided the microsensor with a high sensitivity for detection of abscisic acid with a detection limit of 0.8 nM. With use of this microsensor, an increase of extracellular abscisic acid from single rice protoplast induced by sulfate, osmotic and salinity stress was real-time monitored. Direct measurement of free extracellular abscisic acid in single plant cells might offer important new insights into its role in plants challenged by abiotic stresses.

Electrochemical biosensor for cytokinins based on the CHASE domain of Arabidopsis histidine kinases 4.

In this study, An AHK4 CHASE domain was used to construct an electrochemical cytokinin biosensor using ferrocene as the electrochemical mediator. Upon addition of cytokinin, the binding of cytokinin and AHK4 led to dimerization, which blocked electron transfer between ferrocene and the electrode, and the redox peak current of ferrocene was gradually reduced. Cytokinin was detected by recording the change of the ferrocene redox peak current. The biosensor shows a linear range of 50-400 nM with a linear regression equation of ip = 0.0086c + 0.732 (R2 = 0.993) with ip in µA and c in nM and a detection limit (LOD) of 1.5 nM (S/N = 3). The biosensor exhibits excellent performance that avoids interference of other types of plant hormones and was successfully applied to the detection of cytokinins in bean sprouts.