The impact of urban integration on the intention of settlement and residence among migrants: The empirical evidence from China.

Promoting urban integration is the key to improving the efficiency of labor allocation in developing countries and promoting coordinated regional development. Using the data of the migrants' observation sample in 2012-2017 released by China Migrants Dynamic Survey, this paper draws on theories related to urban integration and labor migration to study the urban integration status, intention of settlement and residence in China's agricultural household labor force in depth, as well as the correlation between the two. The study finds a significant decline in the intention of settlement and a gradual increase in the intention of residence among the migrants. The empirical regressions suggest that good urban integration, especially psychological integration, mainly contributes to their intention of settlement and residence, while the intention of residence is also more influenced by the social and economic integration dimensions. The mediating effect tests reveal that income level and housing stability are key pathways to economic integration, public medical participation and accessibility to medical services are key pathways to health integration, social status and local attachment are key pathways to social integration, and identity affiliation and psychological assimilation are key pathways to psychological integration. Future urbanization in developing countries requires not only further reform of the household registration system but also government, business, and social organizations at all levels to reduce the difficulties of urban integration in economic, health, social, and psychological aspects, enhance the degree of urban integration of the migrants, increase the intention of the migrants to settle and stay in different places and avoid the "migratory bird" migration of labor between regions. The "migratory bird" migration between regions is avoided.

TGN-020 Alleviate Inflammation and Apoptosis After Cerebral Ischemia-Reperfusion Injury in Mice Through Glymphatic and ERK1/2 Signaling Pathway.

Post-stroke acute inhibition of aquaporin 4 (AQP4) is known to exacerbate inflammation and apoptosis, yet the underlying mechanisms are not fully understood. The objective of this study was to investigate the specific mechanism of inflammation and apoptosis following cerebral ischemia-reperfusion (I/R) injury using the AQP4-specific inhibitor, N-(1,3,4-thiadiazol-2-yl) pyridine-3-carboxamide dihydrochloride (TGN-020). Ischemic stroke was induced in mice using the middle cerebral artery occlusion (MCAO) model. The C57/BL6 mice were randomly divided into three groups as follows: sham operation, I/R 48 h, and TGN-020 + I/R 48 h treatment. All mice were subjected to a series of procedures. These procedures encompassed 2,3,5-triphenyltetrazolium chloride (TTC) staining, neurological scoring, fluorescence tracing, western blotting, immunofluorescence staining, and RNA sequencing (RNA-seq). The glymphatic function in the cortex surrounding cerebral infarction was determined using tracer, glial fibrillary acid protein (GFAP), AQP4 co-staining, and beta-amyloid precursor protein (APP) staining; differential genes were detected using RNA-seq. The influence of TGN-020 on the extracellular signal-regulated kinase 1/2 (ERK) 1/2 pathway was confirmed using the ERK1/2 pathway agonists Ro 67-7467. Additionally, we examined the expression of inflammation associated with microglia and astrocytes after TGN-020 and Ro 67-7467 treatment. Compared with I/R group, TGN-020 alleviated glymphatic dysfunction by inhibiting astrocyte proliferation and reducing tracer accumulation in the peri-infarct area. RNA-seq showed that the differentially expressed genes were mainly involved in the activation of astrocytes and microglia and in the ERK1/2 pathway. Western blot and immunofluorescence further verified the expression of associated inflammation. The inflammation and cell apoptosis induced by I/R are mitigated by TGN-020. This mitigation occurs through the improvement of glymphatic function and the inhibition of the ERK1/2 pathway.

The impact of risk perception and preference on farmland transfer-out: Evidence from a survey of farm households in China.

This study examines the impact of farmers' risk perceptions on their decision to transfer-out farmland, as well as the variations in this influence based on different risk preferences. The research utilizes survey data from 1389 farmers in rural China. By expanding the decision-making model for farmer households within the context of risk perception, this paper analyzes the equilibrium point of farmers' decision-making using mathematical models. Employing the Probit and Tobit models with instrumental variable methods, we discovered that farmers' perceptions of land revenue risk, land use risk, and non-farm employment risk significantly hindered the decision and rate of farmland transfer-out. Notably, the inhibitory effect decreased progressively. Moreover, risk preference was found to moderates the negative impacts. However, it is worth noting that risk perception remains a crucial factor impeding farmland transfer, primarily due to the prevalence of neutral or risk-averse risk preferences among most farmers. This study presents a novel perspective and explanatory framework for development of the farmland circulation market, aiming to overcome existing challenges from a risk-oriented standpoint. Consequently, it is essential for the government to carefully address all risks associated with farmland transfer, enhance the income guarantee system for farmers post-transfer, and offer non-farm employment training and job opportunities to facilitate the healthy and rapid development of the agricultural land transfer market.

Limb Remote Ischemic Postconditioning Improves Glymphatic Dysfunction After Cerebral Ischemia-Reperfusion Injury.

BACKGROUND: Delayed neuronal damage can be caused or aggravated after cerebral ischemia-reperfusion (I/R) injury. Recent studies have shown that glymphatic system dysfunction after cerebral ischemia-reperfusion injury is involved in ischemic brain edema and neuroinflammation, thereby regulating cerebral ischemia-reperfusion injury. The aim of this study is to investigate the changes of glymphatic system after cerebral ischemia-reperfusion injury and whether limb remote ischemic postconditioning (LRIP) can improve the function of glymphatic system to protect the brain. METHODS: To establish a focal brain I/R injury mouse model, this study utilized the middle cerebral artery occlusion/reperfusion (MCAO/R) method. The present study classified eight-week-old C57BL/6 male mice into three groups. The changes in glymphatic function in different periods of ischemia and reperfusion were analyzed through immunofluorescence, transmission electron microscopy (TEM), and Western-Blot (WB) assays. The contents of the evaluation included cerebrospinal fluid flow, swelling degree of brain tissue, aquaporin-4 (AQP4) expression and polarization, and amyloid-ß (Aß) excretion. RESULTS: In the early stages of cerebral ischemia, cerebrospinal fluid (CSF) flow is disturbed, accompanied by a decrease in AQP4 polarization. The polarity of AQP4 decreased from 12 h to 72 h of reperfusion, the Aß deposition. LRIP can increase the expression of ß-DG and AQP4 polarization, reduce the deposition of Aß, improve the function of the glymphatic system, and reduce the expression of AQP4 to play A protective role in brain. CONCLUSION: Glymphatic system impaired after cerebral ischemia-reperfusion injury in mice. LRIP may play a neuroprotective role by improving glymphatic function after I/R.

Correlation Analysis of Neutrophil/Albumin Ratio and Leukocyte Count/Albumin Ratio with Ischemic Stroke Severity.

Ischemic stroke (IS) is a common neurological disease in the elderly, but the relationship between neutrophil/albumin ratio (NAR) and leukocyte count/albumin ratio (LAR) and the severity of neurological function injury and early neurological deterioration (END) occurrence remain elusive in acute IS. A total of 299 patients with acute IS and 56 healthy controls were enrolled. According to the NIHSS score at admission, the disease group was divided into three groups (mild, moderate and severe IS), and the differences in five indexes NAR, LAR, neutrophil count, leukocyte count and albumin among the four groups were analyzed. Furthermore, explore the correlation between the above indicators and the severity of IS and END occurrence. The results showed that higher NAR, LAR, neutrophil count, leukocyte count levels and lower albumin levels were associated with acute IS, and the levels of NAR and LAR increased gradually in three groups of IS. NAR and LAR were positively and albumin was negatively correlated with the severity of IS. Meanwhile, NAR and LAR showed a good predictive value in identifying patients with END after acute IS. NAR and LAR may be predictors of the severity of IS and END occurrence after acute IS.

Universal Flexible Lamination Encapsulation Strategy toward Underwater-Operation Electroluminescence Devices.

A reliable encapsulation technology with scalability and flexibility is urgently needed for electroluminescence devices. Here, we developed a simple, robust, low-cost, and scalable flexible lamination encapsulation strategy with quantum-dot light-emitting diodes (QLEDs) as the model devices. Multilayered Parafilm combining with calcium oxide buffer was used for the lamination encapsulation. We successfully demonstrated that such a Parafilm Lami encapsulation (PLE) not only allowed excellent protection for QLEDs in air but endowed QLED outstanding waterproof performance. As a result, highly efficient and stable flexible waterproof QLEDs were realized based on this PLE, exhibiting maximum external quantum efficiency of ∼8% and long half-luminescence lifetime of over 1.5 h in water. We believe that there are not any obstacles to extending this encapsulation technology to other flexible flat-panel devices, such as organic/perovskite light-emitting diodes.

Research on the promoting effect of servitization on export technological sophistication of manufacturing enterprises.

Based on multiple micro databases involving Chinese manufacturing enterprises and World Input-Output Database, this article investigates the impact of China's manufacturing servitization on export technological sophistication from 2000 to 2010. The results show that manufacturing servitization has an inverted U-shaped impact on export technological sophistication. From the perspective of heterogeneity at the enterprise level and industry level, manufacturing servitization has an inverted U-shaped impact on export technological sophistication for mixed trade enterprises, central and western located enterprises, domestic and foreign enterprises, and knowledge-intensive industries, the nonlinear impact is in the promotion range. Besides, manufacturing servitization with domestic and foreign service input source has an inverted U-shaped impact on export technological sophistication, manufacturing servitization with the domestic consumption-oriented service input source and foreign production-oriented service input source have a promoting effect. Servitization with financial industry and technical research and development service source has a promoting effect, while servitization with transportation service input source has an inverted U-shaped effect. Overall global value chain participation level and simple global value chain participation have a positive moderating effect on the impact, especially for enterprises with lower production efficiency. Mechanism analysis confirms that the "spillover" effect and "cost" effect are important channels for manufacturing servitization to promote export technological sophistication.

Molecular characterization, expression analysis and function identification of Pf_TNF-α and its two receptors Pf_TNFR1 and Pf_TNFR2 in yellow catfish (Pelteobagrus fulvidraco).

Inflammation is a common manifestation of body immunity and mediates a cascade of cytokines. Tumor necrosis factor-α (TNF-α), as a multi-effect cytokine, plays an important role in the inflammatory response by interacting with its receptor (TNFR). In this study, Pf_TNF-α, Pf_TNFR1 and Pf_TNFR2 genes were cloned from yellow catfish (Pelteobagrus fulvidraco), and bioinformatics analyses showed that the three genes were conserved and possessed similar sequence characteristics as those of other vertebrates. The qPCR results showed that Pf_TNF-α, Pf_TNFR1 and Pf_TNFR2 mRNAs were constitutively expressed in 14 tissues and the lymphocytes of four tissues from healthy adults. The mRNA expression levels of Pf_TNF-α and Pf_TNFR1 genes were significantly up-regulated in the spleen, liver, trunk kidney, head kidney and gill after Edwardsiella ictaluri infection, while the mRNA expression of Pf_TNFR2 was significantly up-regulated in the spleen, and down-regulated in the liver and gill. In the isolated peripheral blood leukocytes (PBLs) of yellow catfish, the expression of Pf_TNF-α mRNA was notably up-regulated and the two Pf_TNFR transcripts were distinctly down-regulated after stimulation with lipopolysaccharides (LPS), peptidoglycan (PGN), polyinosinic-polycytidylic acid (Poly I:C) and phytohaemagglutinin (PHA). After stimulated by recombinant (r) Pf_sTNF protein, the mRNA expressions of various inflammatory factors genes were up-regulated in the PBLs. Meanwhile, rPf_sTNF promoted the phagocytic activity of leukocytes, whereas the activity mediated by rPf_sTNF could be inhibited by rPf_TNFR1CRD2/3 and rPf_TNFR2CRD2/3. The up-regulation of TNF-α and IL-1ß mRNAs expression triggered by rPf_sTNF could be inhibited by MAPK inhibitor (VX-702) and NF-κB inhibitor (PDTC). rPf_sTNF induced the expression of FADD mRNA in PBLs and increased the apoptotic rate of PBLs, and inhibiting the NF-κB and MAPK signal pathways could enhance the apoptosis of PBLs. The results indicate that Pf_TNF-α, Pf_TNFR1 and Pf_TNFR2 play important roles in the immune response of yellow catfish to bacterial invasion.

Environmental regulation, green innovation, and international competitiveness of manufacturing enterprises in China: From the perspective of heterogeneous regulatory tools.

Based on combined data from the China Patent Database, China Industrial Enterprise Database, and China Customs Import and Export Database for the period 2004-2010, this study investigates the impact of heterogeneous environmental regulations on the export technological sophistication of manufacturing enterprises. Given deepening international market segmentation of production and the increasing proportion of intermediate trade, and compared with the traditional method based on exports, the export technological sophistication calculated here, based on value-added, is closer to the true level. Since there has been no in-depth comparative study on the relationship between heterogeneous environmental regulation and export technological sophistication, this study fills the gap. The results show that all three regulation types bear a U-shaped impact on export technological sophistication. Command-control regulation exhibits a restraining effect on mixed trade, eastern, and foreign-funded enterprises. Market-incentive regulation promotes processing and mixed trade enterprises as well as domestic and foreign-funded enterprises. Voluntary-participation regulation promotes all enterprises with different trade patterns and ownership. The mechanism analysis shows that command-control and market-participation environmental regulations affect export technological sophistication through the green invention and green utility innovation channels, while, additionally, market-incentive environmental regulation affects export technological sophistication through the green design innovation channel. Considering the environmental governance issues, the policy implications for enhancing the entire industrial chain and enterprises' export competitiveness are clear. Due to the unclear functions and powers of competent departments and a rigid threshold, command-control regulation is not conducive to cleaner production technology and the promotion of enterprises' export competitiveness; it should thus be discouraged. Although both market-incentive and voluntary-participation regulations have promoted cleaner production technology and enterprises' competitiveness significantly, the environmental tax system requires continuous improvement. The government should continue to raise public involvement in environmental protection to enrich the channels and forms of environmental management.

Experimental equations of seawater salinity and desalination capacity to assess seawater irrigation.

A central question in science and technology of desalting is, can we predict optimal coastal sites to implement seawater irrigation? Freshwater only makes up 2.5% of all water on Earth but crop irrigation is responsible for 70% of freshwater demand. First, we compared the growth rates and the dehydration rates of 5 alternative seawater irrigation experiments of wheatgrass over 3 weeks' periods. The average salt tolerance threshold of wheatgrass is 6 dS m-1. When seawater salinity is increased >10.50 dS m-1, the growth, drainage volumes, leaching, and drainage salinities of wheatgrass did not show significant variations. When seawater salinity is increased to 12.25 dS m-1, grass leaves gradually turned light green, bent, and fell. Notably, pH in soil remained nearly constant in all experiments with mean pH of 6.05 ±â€¯0.25 (mean ±â€¯SD). Next, we derived experimental equations to define a mechanistic link between salinity and desalination capacity in a Modified Saline Adjustable Desalination System (MSADS). A cost-benefit analysis for a MSADS in a coastal location of southern California indicated that this system is $0.84 m-3 more expensive than using water from a natural reservoir, but $0.08 m-3 less expensive than importing water. This study provides a general framework to assess the implementation of a desalination system in coastal locations.