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Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 14(3): 614-6, 2006 Jun.
Article in Chinese | MEDLINE | ID: mdl-16800954

ABSTRACT

To evaluate flow-cytometry and Nageotte method for counting residual WBC in thrombocytaphoresis concentrates, their accuracies were determined by dilution studies separately; the repeatability was determined by measuring the interassay coefficient of variation for 14 replicates of a sample with known leukocyte concentration. 102 samples of leukocyte-depleted thrombocytaphoresis concentrates were detected by the above mentioned two methods, and the results were compared each other. The results showed that no difference was observed between two methods over a range of leukocyte concentrations from 0.8 to 10 WBC/microl (P > 0.05). In conclusion, flow-cytometry and Nageotte methods can be used for quality control of WBC-reduced blood components.


Subject(s)
Leukocyte Count/methods , Leukocyte Reduction Procedures , Plateletpheresis , Blood Component Transfusion , Evaluation Studies as Topic , Flow Cytometry , Humans , Leukocyte Reduction Procedures/methods
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