ABSTRACT
The pandemic of COVID-19 is now posing a worldwide hazard to one's health. Exposure to COVID-19 may cause negative emotions like anxiety, which is one of recognized risk factors for aggressive behaviors. This study looked into the effect of exposure to COVID-19 on aggression and how anxiety may act as a mediating factor, as well as lastly how rumination could moderate a variety of indirect paths during the epidemic of COVID-19. According to the current study's findings, which included a sizable sample of Chinese college students (N = 1,518), being exposed to COVID-19 showed a positive connection with aggression and anxiety, as well as rumination. These findings clarify the role that mediators play in the relationship between anxiety and exposure to COVID-19. The results are also helpful for personalizing treatments and putting preventative measures in place to decrease the aggression brought on by exposure to COVID-19. It is explored how lowering rumination and anxiety may be useful in the context of COVID-19 to lessen the psychopathological effects of the condition.
ABSTRACT
The present study examined a moderated mediation model between life satisfaction and suicidal ideation among Chinese college students during the recurrent outbreak of COVID-19. Seven hundred and ninety college students participated in the study and completed questionnaires on life satisfaction, suicidal ideation, depression, and gratitude. Findings indicated that (1) Depression played a partial mediating role between life satisfaction and suicidal ideation. Life satisfaction was not only directly affected suicidal ideation, but also indirectly affected suicidal ideation through the mediating effect of college students' depression; (2) Gratitude played a moderating role in the effect of life satisfaction on depression, and the link between life satisfaction and suicide ideation was only significant for those with higher gratitude. This study provides practical implications for the prevention of suicidal behavior among Chinese college students during the recurrent outbreak of COVID-19.
ABSTRACT
OBJECTIVE: To set up an effective and simple purification method to obtain highly purified prokaryotic protein of PDCD5 and study its stability. METHODS: Recombinant PDCD5 protein expressed in E. coli. was accumulated as an inclusion body. After washing, the inclusion body was denatured, renatured, digested with thrombine and then purified by two steps of chromatography. The purity of the products was analyzed by capillary electrophoresis and the stability was identified by SDS-PAGE. RESULTS: Capillary electrophoresis showed that the purity of protein was 100%, and molecular weight was 15,800 with pI 5.9. Further bioactivity assay indicated that the purified PDCD5 could enhance the apoptosis of HL-60 cells withdrawing cytokine, which was in a dose-dependent manner. Stability analysis showed that the PDCD5 protein was sensitive to temperature and easy to degrade at 4 degrees C and 25 degrees C. However, it was relatively stable at -20 degrees C or lyophilized. CONCLUSION: Highly purified and stable recombinant PDCD5 protein was obtained, which lays a foundation for the functional study and application investigation of PDCD5.
