ABSTRACT
OBJECTIVE: This study sought to reveal the relationship between Snap-25 and tetramine poisoning in order to approach the mechanism of tetramine poisoning. METHODS: The levels of Snap-25 mRNA in the brain tissues of poisoned SD rats were detected by RT-PCR method. RESULTS: After 1 hour of intoxication, the expression level of Snap-25 mRNA did not change in the rat brain. It increased at day 1 and reached its peak at day 3. The expression level of Snap-25 mRNA begain to descend at day 5, but it was still higher than that in the rat brain tissue of control group. CONCLUSION: In this study the expression of Snap-25 of rat poisoned by tetramine was not markedly increased in the early stage of poisoning, indicating that the symptoms of rat poisoned by tetramine are not induced by the changes of the Snap-25 level. The mechanism of high expression in Snap-25 mRNA probably involves the negative feedback of the body, which may increase the releasing of neurohumor and hence mitigate the symptoms of poisoning.
Subject(s)
Brain/metabolism , Bridged-Ring Compounds/poisoning , Membrane Proteins/biosynthesis , Nerve Tissue Proteins/biosynthesis , Animals , Male , Membrane Proteins/genetics , Nerve Tissue Proteins/genetics , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Rats , Rats, Sprague-Dawley , Synaptosomal-Associated Protein 25ABSTRACT
The Cardiac Troponin T and I are highly cardiac specific biochemical markers of myocardial injury. They are very sensitive markers to detect all kinds of myocardial injury, and are able to distinguish myocardial injury and skeletal injury. Furthermore, They are independent predictor of future cardiac events. Such markers are now widely used in the clinic practice. It is prospective to use them in Forensic Medical Science.
Subject(s)
Forensic Medicine , Myocardial Infarction/blood , Troponin I/blood , Troponin T/blood , Biomarkers , Humans , Myocardium/metabolismABSTRACT
OBJECTIVE: To evaluate changes of VEGF in skin after blunt injury. METHODS: The rats of injury groups were subjected to skin blunt injury by free-falling iron hammer. The samples taken at 1 h, 3 h, 6 h, 12 h, 1 d, 3 d, 5 d, 7 d, 9 d after injury were studied by immunohistochemistry and MIAS image analysis system. RESULTS: In the skin of normal control group the expression level of VEGF was low. The increase of VEGF could be observed at 1 day after injury, reached peak at 7 days and declined at 9 days. CONCLUSION: Blunt injury in the skin could induce the expression of the VEGF. Moreover, the change pattern of VEGF level was quite regular with time and could be used to estimate the time after skin injury.
Subject(s)
Endothelial Growth Factors/metabolism , Intercellular Signaling Peptides and Proteins/metabolism , Lymphokines/metabolism , Skin/injuries , Wounds, Nonpenetrating/metabolism , Animals , Female , Forensic Medicine , Male , Rats , Rats, Sprague-Dawley , Skin/metabolism , Time Factors , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth FactorsABSTRACT
Calcitonin gene-related peptide (CGRP) play a key role in some physiological and pathological progresses. The latest studies indicate that CGRP might involve in some disease progress and has a close relation with wound healing. It is significant to further investigate and then apply it to clinical diagnosis and therapy as well as forensic pathology.
