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1.
Blood Cancer J ; 5: e303, 2015 Apr 10.
Article in English | MEDLINE | ID: mdl-25860294

ABSTRACT

We examined genetic and epigenetic changes that occur during disease progression from indolent to aggressive forms of chronic lymphocytic leukemia (CLL) using serial samples from 27 patients. Analysis of DNA mutations grouped the leukemia cases into three categories: evolving (26%), expanding (26%) and static (47%). Thus, approximately three-quarters of the CLL cases had little to no genetic subclonal evolution. However, we identified significant recurrent DNA methylation changes during progression at 4752 CpGs enriched for regions near Polycomb 2 repressive complex (PRC2) targets. Progression-associated CpGs near the PRC2 targets undergo methylation changes in the same direction during disease progression as during normal development from naive to memory B cells. Our study shows that CLL progression does not typically occur via subclonal evolution, but that certain CpG sites undergo recurrent methylation changes. Our results suggest CLL progression may involve developmental processes shared in common with the generation of normal memory B cells.


Subject(s)
Clonal Evolution/genetics , DNA Methylation/genetics , Epigenesis, Genetic , Leukemia, Lymphocytic, Chronic, B-Cell/genetics , CpG Islands/genetics , Disease Progression , Humans , Leukemia, Lymphocytic, Chronic, B-Cell/pathology , Mutation , Polycomb-Group Proteins/genetics
2.
J Intern Med ; 274(5): 440-50, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23751076

ABSTRACT

Genomic variation, through effects on gene structure and expression, plays an important role in understanding disease predisposition, biology and clinical response to therapy. Transforming this knowledge into clinically relevant information that tailors interventions to an individual's specific genetic, physical, social and environmental profile is challenging. To illustrate how research initiatives at preclinical phases of development are attempting to address clinically important issues in oncology, six clinical problems related to cancers of the colon, prostate, breast, pancreas and brain (medulloblastoma) as well as metastatic disease of different origins are described. A unifying theme across applications is that healthy individuals previously indistinguishable in regards to cancer risk and patients with cancer previously categorized as similar with regard to prognosis or drug response are being stratified into more refined subgroups with different clinical profiles. Effective matching of a broad range of tests with more tailored strategies for prevention and/or treatment will require well-designed clinical studies to evaluate benefits and costs.


Subject(s)
Genetic Variation/genetics , Neoplasms/genetics , Precision Medicine/methods , Cerebellar Neoplasms/genetics , Colorectal Neoplasms/diagnosis , Colorectal Neoplasms/genetics , Early Detection of Cancer , Genetic Predisposition to Disease/genetics , Humans , Male , Medulloblastoma/genetics , Neoplasm Metastasis/genetics , Pancreatic Neoplasms/genetics , Pancreatic Neoplasms/mortality , Prostatic Neoplasms/diagnosis , Prostatic Neoplasms/genetics , Survivors
3.
Allergy ; 64(5): 746-53, 2009 May.
Article in English | MEDLINE | ID: mdl-19254290

ABSTRACT

BACKGROUND: Serum immunoglobulin E (IgE) level is recognized to be under strong genetic control, but the causal and susceptibility genes remain to be identified. We sought to investigate the association between single nucleotide polymorphisms (SNPs) in the Toll-like receptor (TLR) signaling pathway and total serum IgE level. METHODS: A population of 206 patients with severe chronic rhinosinusitis (CRS) was used. Precise phenotyping of patients was accomplished by means of a questionnaire and clinical examination. Blood was drawn for measurement of total serum IgE, as well as DNA extraction. A maximally informative set of SNPs in the TLR1, 2, 3, 4, 6, 9, 10, CD14, MD2, MyD88, IRAK4, and TRAF6 genes were selected and genotyped. Significant findings were replicated in a second independent population of 956 subjects from 227 families with asthma. RESULTS: A total of 97 out of 104 SNPs were successfully genotyped. Three SNPs in IRAK4--rs1461567, rs4251513, and rs4251559--were associated with total serum IgE levels (P < 0.004). In the replication sample, the same SNPs as well as the same orientation of the risk allele were associated with IgE levels (P < 0.031). CONCLUSIONS: These results demonstrate a clear association between polymorphisms in the IRAK4 gene and serum IgE levels in patients with CRS and asthma. IRAK4 may be important in the regulation of IgE levels in patients with inflammatory diseases of the airways.


Subject(s)
Gene Frequency/genetics , Hypersensitivity/genetics , Immunoglobulin E/blood , Interleukin-1 Receptor-Associated Kinases/genetics , Adult , Chronic Disease , Cross-Sectional Studies , Female , Gene Frequency/immunology , Genotype , Humans , Hypersensitivity/immunology , Interleukin-1 Receptor-Associated Kinases/immunology , Linkage Disequilibrium , Male , Middle Aged , Polymorphism, Single Nucleotide , Surveys and Questionnaires
4.
Genes Immun ; 9(2): 93-102, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18216865

ABSTRACT

Systemic lupus erythematosus (SLE) is a complex disease trait of unknown aetiology. Genome-wide linkage studies in human SLE identified several linkage regions, including one at 1q23, which contains multiple susceptibility genes, including the members of the signalling lymphocyte activation molecule (SLAM) locus. In mice there is a syntenic linkage region, Sle1. The SLAM genes are functionally related cell-surface receptors, which regulate signal transduction of cells in the immune system. Family-based association study in UK and Canadian SLE families identified variants in the promoter and coding region of SLAMF7 and LY9 contributing to SLE disease susceptibility. The strongest association was from rs509749, in exon 8 of LY9 (P=0.00209). rs509749 encodes a Val/Met nonsynonymous change in amino acid 602 in the cytoplasmic domain of LY9. In the parents and affected individuals from the Canadian SLE families, the risk allele of rs509049 skews the T-cell population by increasing the number of CD8+ memory T cells, while decreasing the proportion of CD4+ naïve T cells and activated T cells. Since rs509749 lies within the consensus binding site for SAP/SH2D1a, which influences downstream signalling events from LY9, the mechanism for increased CD8+ memory T cells may include differential binding SAP/SH2D1a to the cytoplasmic domain of LY9.


Subject(s)
Alleles , Antigens, CD/genetics , Genetic Linkage/genetics , Lupus Erythematosus, Systemic/genetics , Membrane Glycoproteins/genetics , Canada/epidemiology , Genetic Predisposition to Disease , Humans , Lupus Erythematosus, Systemic/epidemiology , Pedigree , Polymorphism, Single Nucleotide/genetics , Signaling Lymphocytic Activation Molecule Family , United Kingdom/epidemiology
5.
Proc Natl Acad Sci U S A ; 104(52): 20896-901, 2007 Dec 26.
Article in English | MEDLINE | ID: mdl-18087043

ABSTRACT

Multiple sclerosis (MS) is a common inflammatory disease of the central nervous system unsurpassed for variability in disease outcome. A cohort of sporadic MS cases (n = 163), taken from opposite extremes of the distribution of long-term outcome, was used to determine the role of the HLA-DRB1 locus on MS disease severity. Genotyping sets of benign and malignant MS patients showed that HLA-DRB1*01 was significantly underrepresented in malignant compared with benign cases. This allele appears to attenuate the progressive disability that characterizes MS in the long term. The observation was doubly replicated in (i) Sardinian benign and malignant patients and (ii) a cohort of affected sibling pairs discordant for HLA-DRB1*01. Among the latter, mean disability progression indices were significantly lower in those carrying the HLA-DRB1*01 allele compared with their disease-concordant siblings who did not. The findings were additionally supported by similar transmission distortion of HLA-DRB1*04 subtypes closely related to HLA-DRB1*01. The protective effect of HLA-DRB1*01 in sibling pairs may result from a specific epistatic interaction with the susceptibility allele HLA-DRB1*1501. A high-density (>700) SNP examination of the MHC region in the benign and malignant patients could not identify variants differing significantly between the two groups, suggesting that HLA-DRB1 may itself be the disease-modifying locus. We conclude that HLA-DRB1*01, previously implicated in disease resistance, acts as an independent modifier of disease progression. These results closely link susceptibility to long-term outcome in MS, suggesting that shared quantitative MHC-based mechanisms are common to both, emphasizing the central role of this region in pathogenesis.


Subject(s)
Gene Expression Regulation , HLA-DR Antigens/genetics , Multiple Sclerosis/diagnosis , Multiple Sclerosis/genetics , Adult , Alleles , Disease Progression , Female , Gene Frequency , Genetic Predisposition to Disease , HLA-DRB1 Chains , Humans , Italy , Male , Middle Aged , Models, Genetic , Phenotype , Polymorphism, Single Nucleotide , Treatment Outcome
6.
Int J Obes (Lond) ; 31(8): 1325-33, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17342071

ABSTRACT

BACKGROUND: Abdominal obesity and hypertriglyceridemia (the hypertriglyceridemic-waist phenotype) increase cardiovascular risk. The very low-density lipoprotein (VLDL) is a triglyceride (TG)-rich particle. Frequent variations in the genes coding for enzymes and proteins involved in the VLDL catabolism have already been documented. The epistatic effect of such variants on the risk profile associated with abdominal obesity remains to be elucidated. OBJECTIVE: This study aims to assess the effect of combinations of frequent single-nucleotide polymorphisms (SNPs) in the VLDL catabolic pathway on the relation between abdominal obesity and fasting TG. METHOD: Only gene variants in the lipoprotein lipase, apolipoprotein (apo) CIII, hepatic lipase and apo E genes known to be frequent in the general population (allele frequency>5%) were included in this study. The presence of selected SNPs was detected by polymerase chain reaction-restriction fragment length polymorphism in a sample of 640 non-diabetic French Canadians at high cardiovascular risk (405 obese, 235 non-obese). RESULTS: Carrying more than two frequent gene variants involved in the VLDL catabolic pathway significantly increased the risk of hyperTG (odds ratio of TG>1.7 mmol/l=4.15; P=0.001). This effect was proportional to the number of SNPs and genes involved and was significantly amplified by the presence of abdominal obesity defined on the basis of waist circumference. CONCLUSION: When combined with abdominal obesity, epistasis in the VLDL pathway has a deleterious effect on fasting TG and coronary artery disease risk profile according to the TG threshold (1.7 mmol/l) used in medical guidelines for the assessment of the metabolic syndrome and associated risk.


Subject(s)
Epistasis, Genetic , Hypertriglyceridemia/genetics , Lipoproteins, VLDL/metabolism , Obesity/genetics , Apolipoprotein C-III/genetics , Apolipoprotein C-III/metabolism , Apolipoprotein E2/genetics , Apolipoprotein E2/metabolism , Apolipoprotein E4/genetics , Apolipoprotein E4/metabolism , Canada , Female , France/ethnology , Humans , Hypertriglyceridemia/ethnology , Hypertriglyceridemia/metabolism , Lipase/genetics , Lipase/metabolism , Lipoprotein Lipase/genetics , Lipoprotein Lipase/metabolism , Male , Middle Aged , Obesity/ethnology , Obesity/metabolism , PPAR alpha/genetics , PPAR alpha/metabolism , PPAR gamma/genetics , PPAR gamma/metabolism , Phenotype , Triglycerides/blood , Waist-Hip Ratio
7.
Genes Immun ; 7(8): 632-9, 2006 Dec.
Article in English | MEDLINE | ID: mdl-17082760

ABSTRACT

CX3CR1, a fractalkine receptor, mediates cell-adhesive and migratory functions in inflammation. Based on CX3CR1 expression observed in bronchial tissues of asthmatic subjects, we hypothesized that genetic variation at this locus may affect susceptibility to asthma. We carried out an association study and a haplotypic analysis with selected polymorphisms of the CX3CR1 in a familial asthmatic sample from a founder population. Genetic analyses performed by FBAT software showed five CX3CR1 single nucleotide polymorphisms (rs938203, rs2669849, rs1050592, T280M and V249I) with significant associations between their common alleles and asthma (P<0.004) in a dominant model. A haplotype formed with common alleles of rs1050592, T280M and V249I is also overtransmitted in asthmatic subjects (P=0.005) under a dominant model. The associations of V249I and rs2669849 have been validated in an independent case-control sample. For V249I, odds ratios (OR) are 2.16 (common homozygous) and 2.11 (heterozygous) in dominant model (P=0.031). For rs2669849, OR are 2.75 (common homozygous) and 1.86 (heterozygous) in additive model (P=0.007) and dominant model (P=0.059). These results suggest an asthma protective effect of the minor alleles in healthy control carriers. Further functional studies of CX3CR1 are needed to document its role in the pathophysiology of asthma.


Subject(s)
Asthma/genetics , Genetic Predisposition to Disease , Genetic Variation , Receptors, Chemokine/genetics , Adolescent , Adult , CX3C Chemokine Receptor 1 , Female , Gene Components , Haplotypes/genetics , Humans , Linkage Disequilibrium , Lung/metabolism , Male , Middle Aged , Odds Ratio , Polymorphism, Single Nucleotide/genetics , Quebec , Receptors, Chemokine/metabolism
8.
Br J Cancer ; 94(3): 436-45, 2006 Feb 13.
Article in English | MEDLINE | ID: mdl-16421595

ABSTRACT

In order to elucidate the biological variance between normal ovarian surface epithelial (NOSE) and epithelial ovarian cancer (EOC) cells, and to build a molecular classifier to discover new markers distinguishing these cells, we analysed gene expression patterns of 65 primary cultures of these tissues by oligonucleotide microarray. Unsupervised clustering highlights three subgroups of tumours: low malignant potential tumours, invasive solid tumours and tumour cells derived from ascites. We selected 18 genes with expression profiles that enable the distinction of NOSE from these three groups of EOC with 92% accuracy. Validation using an independent published data set derived from tissues or primary cultures confirmed a high accuracy (87-96%). The distinctive expression pattern of a subset of genes was validated by quantitative reverse transcription-PCR. An ovarian-specific tissue array representing tissues from NOSE and EOC samples of various subtypes and grades was used to further assess the protein expression patterns of two differentially expressed genes (Msln and BMP-2) by immunohistochemistry. This study highlights the relevance of using primary cultures of epithelial ovarian cells as a model system for gene profiling studies and demonstrates that the statistical analysis of gene expression profiling is a useful approach for selecting novel molecular tumour markers.


Subject(s)
Biomarkers, Tumor/genetics , Gene Expression Profiling , Ovarian Neoplasms/classification , Ovarian Neoplasms/diagnosis , Biomarkers, Tumor/metabolism , Bone Morphogenetic Protein 2 , Bone Morphogenetic Proteins/analysis , Bone Morphogenetic Proteins/genetics , Cells, Cultured , Epithelial Cells/cytology , Epithelial Cells/pathology , Female , GPI-Linked Proteins , Genes, Neoplasm , Humans , Membrane Glycoproteins/analysis , Membrane Glycoproteins/genetics , Mesothelin , Neoplasm Staging , Ovarian Neoplasms/genetics , Ovary/chemistry , Ovary/cytology , Ovary/pathology , Receptors, Tumor Necrosis Factor, Type I/analysis , Receptors, Tumor Necrosis Factor, Type I/genetics , Sodium-Potassium-Exchanging ATPase/analysis , Sodium-Potassium-Exchanging ATPase/genetics , Transforming Growth Factor beta/analysis , Transforming Growth Factor beta/genetics , Tumor Cells, Cultured
9.
J Med Genet ; 43(2): 129-32, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16014697

ABSTRACT

BACKGROUND: The 2',5'-oligoadenylate synthetase genes (OAS1, OAS2, and OAS3) map to human chromosome 12q24 and encode a family of enzymes pivotal to innate antiviral defence. Recently, the minor allele of an OAS1 single nucleotide polymorphism (SNP) that alters splicing (rs10774671) was found to be associated with increased enzymatic activity and, in a case-sibling control study, with type 1 diabetes (T1D). METHODS: We have confirmed this T1D association in 784 nuclear families (two parents and at least one affected offspring) by the transmission disequilibrium test (TDT; G:A = 386:329, p = 0.033). However, because of linkage disequilibrium within OAS1 and with the other two OAS genes, functional attribution of the association to this SNP cannot be assumed. To help answer this question, we also genotyped two non-synonymous SNPs in OAS1 exons 3 and 7. RESULTS: All three SNPs showed significant transmission distortion. Three of the eight possible haplotypes accounted for 98.4% of parental chromosomes and two of them carried the non-predisposing A allele at rs10774671. Parents heterozygous for these two haplotypes showed significant transmission distortion (p = 0.009) despite being homozygous at rs10774671. CONCLUSIONS: We confirm the T1D association with rs10774671, but we conclude that it cannot be attributed (solely) to the splicing variant rs10774671. A serine/glycine substitution in OAS1 exon 3 is more likely a functional variant.


Subject(s)
2',5'-Oligoadenylate Synthetase/genetics , Diabetes Mellitus, Type 1/genetics , Haplotypes/genetics , Multigene Family/genetics , Polymorphism, Genetic/genetics , RNA Splicing/genetics , Adolescent , Genetic Predisposition to Disease , Humans , Linkage Disequilibrium
10.
Genes Immun ; 6(6): 519-27, 2005 Sep.
Article in English | MEDLINE | ID: mdl-15988535

ABSTRACT

Reduced infection by mycobacteria, including Mycobacterium tuberculosis, may be partly responsible for increased prevalence of allergic and autoimmune diseases in developed countries. In a murine model of innate resistance to mycobacteria, the Nramp1 gene has been shown to affect asthma susceptibility. From this observation, it was proposed that human NRAMP1 may be a modulator of asthma risk in human populations. To experimentally test the candidacy of NRAMP1 in asthma susceptibility, we characterized five genetic variants of NRAMP1 (5'CAn, 274C>T, 469+14G>C, D543N, and 1729+del4) in an asthma family-based cohort from northeastern Quebec. We did not observe any significant association between NRAMP1 variants (either allele or haplotype specific) with asthma, atopy, or serum immunoglobulin E levels. These results demonstrate that, in spite of direct involvement of Nramp1 in a murine asthma model, in human populations NRAMP1 is not likely to be a major contributor to the genetic etiology of asthma and asthma-related phenotypes.


Subject(s)
Asthma/genetics , Cation Transport Proteins/genetics , Genetic Predisposition to Disease , Hypersensitivity, Immediate/genetics , Polymorphism, Single Nucleotide , Canada , Genotype , Haplotypes , Humans , Immunoglobulin E/blood , White People/genetics
11.
Am J Hum Genet ; 76(5): 815-32, 2005 May.
Article in English | MEDLINE | ID: mdl-15800845

ABSTRACT

The Saguenay-Lac St-Jean population of Quebec is relatively isolated and has genealogical records dating to the 17th-century French founders. In 120 extended families with at least one sib pair affected with early-onset hypertension and/or dyslipidemia, we analyzed the genetic determinants of hypertension and related cardiovascular and metabolic conditions. Variance-components linkage analysis revealed 46 loci after 100,000 permutations. The most prominent clusters of overlapping quantitative-trait loci were on chromosomes 1 and 3, a finding supported by principal-components and bivariate analyses. These genetic determinants were further tested by classifying families by use of LOD score density analysis for each measured phenotype at every 5 cM. Our study showed the founder effect over several generations and classes of living individuals. This quantitative genealogical approach supports the notion of the ancestral causality of traits uniquely present and inherited in distinct family classes. With the founder effect, traits determined within population subsets are measurably and quantitatively transmitted through generational lineage, with a precise component contributing to phenotypic variance. These methods should accelerate the uncovering of causal haplotypes in complex diseases such as hypertension and metabolic syndrome.


Subject(s)
Founder Effect , Genetic Predisposition to Disease , Hypertension/genetics , Adolescent , Adult , Canada , Female , France/ethnology , Genetic Linkage , Genetic Variation , Humans , Lod Score , Male , Middle Aged , Phenotype , Quantitative Trait, Heritable , White People/genetics
13.
J Med Genet ; 41(12): 941-5, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15591281

ABSTRACT

Mucopolysaccharidosis type IIIC (MPS IIIC, or Sanfilippo syndrome C) is a rare lysosomal storage disorder caused by a deficiency of acetyl-coenzyme A:alpha-glucosaminide-N-acetyltransferase. Patients develop progressive neuropsychiatric problems, mental retardation, hearing loss, and relatively minor visceral manifestations. The pattern of transmission is consistent with an autosomal recessive mode of inheritance. The aim of this study was to find a locus for MPS IIIC using a homozygosity mapping approach. A genomewide scan was performed on DNA from 27 affected individuals and 17 of their unaffected relatives. Additional patients were recruited, and DNA was obtained from a total of 44 affected individuals and 18 unaffected family members from 31 families from 10 countries. A working candidate interval was defined by looking for excess homozygosity in patients compared with their relatives. Additional markers were genotyped in regions of interest. Linkage analysis was performed to support the informal analysis. Inspection of the genomewide scan data showed apparent excess homozygosity in patients compared with their relatives for markers on chromosome 8. Additional genotyping identified 15 consecutive markers (from D8S1051 to D8S2332) in an 8.3 cM interval for which the genotypes of affected siblings were identical in state. A maximum multipoint lod score of 10.61 was found at marker D8S519. A locus for MPS IIIC maps to an 8.3 cM (16 Mbp) interval in the pericentromeric region of chromosome 8.


Subject(s)
Chromosomes, Human, Pair 8 , Mucopolysaccharidosis III/genetics , Centromere , Chromosome Mapping , Female , Genetic Linkage , Genetic Markers , Genotype , Homozygote , Humans , Male , Pedigree
14.
Am J Med Genet B Neuropsychiatr Genet ; 127B(1): 30-4, 2004 May 15.
Article in English | MEDLINE | ID: mdl-15108176

ABSTRACT

As part of an extensive study in the Portuguese Island population of families with multiple patients suffering from bipolar disorder and schizophrenia, we performed an initial genome-wide scan of 16 extended families with bipolar disorder that identified three regions on chromosomes 2, 11, and 19 with genome-wide suggestive linkage and several other regions, including chromosome 6q, also approached suggestive levels of significance. Dick et al. [2003: Am J Hum Genet 73:107-114] recently reported in a study of 250 families with bipolar disorder a maxLOD score of 3.61 near marker D6S1021 on chromosome 6q. This study replicates this finding having detected a peak NPL = 2.02 (P = 0.025) with the same marker D6S1021(104.7 Mb). Higher-density mapping provided additional support for loci on chromosome 6 including marker D6S1021 with an NPL = 2.59 (P = 0.0068) and peaking at marker D6S1639 (125 Mb) with an NPL = 3.06 (P = 0.0019). A similar pattern was detected with higher-density mapping of chromosome 11 with an NPL = 3.15 (P = 0.0014) at marker D11S1883 (63.1 Mb). Simulations at the density of our fine mapping data indicate that less than 1 scan out of 10 would find two such scores genome-wide in the same scan by chance. Our findings provide additional support for a susceptibility locus for bipolar disorder on 6q, as well as, suggesting the importance of denser scans. Published 2004 Wiley-Liss, Inc.


Subject(s)
Bipolar Disorder/genetics , Chromosomes, Human, Pair 11/genetics , Chromosomes, Human, Pair 6/genetics , Genetic Predisposition to Disease/genetics , Genome, Human , Bipolar Disorder/pathology , Chromosome Mapping/methods , Chromosomes, Human, Pair 19/genetics , Chromosomes, Human, Pair 2/genetics , Family Health , Genetic Linkage , Humans , Lod Score , Microsatellite Repeats , Portugal
15.
J Endocrinol Invest ; 27(11): 1003-9, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15754730

ABSTRACT

Obesity and insulin resistance are common features of Type 2 Diabetes. A new protein called resistin has been shown to be secreted by adipocytes in mice and to influence insulin sensitivity. The goal of the present study was to investigate the associations between one polymorphism (g-420C>G) of the human resistin gene and phenotypes related to adiposity and glucose metabolism. We genotyped 725 (including 42 diabetics) adult subjects participating in the Quebec Family Study (QFS) by a minisequencing method. Forty-two were diabetic subjects. Phenotypes measured were: body mass index (BMI) and waist circumference (WC), % body fat (PFAT) and fat mass (FM) assessed by under water weighing, abdominal total, subcutaneous and visceral fat assessed by computed tomography and fasting plasma glucose, insulin and C-peptide and their responses to an oral glucose tolerance test (OGTT). Comparisons between genotypes were performed in non-diabetic men (no.=280) and women (no.=403) separately by analyses of covariance (ANCOVA). Among men, g-420 G homozygotes had less visceral fat (p < 0.05), lower levels of acute insulin responses to an OGTT and lower levels of C-peptide in a fasting state and in responses to an OGTT than carriers of the C allele (p < 0.01). These associations were independent of age and adiposity but were not observed in women. These results suggest that in men, the human resistin gene is associated with reduced amount of visceral obesity and lower insulin secretory responses to a glucose load.


Subject(s)
C-Peptide/blood , Diabetes Mellitus, Type 2/genetics , Hormones, Ectopic/genetics , Insulin Resistance/genetics , Obesity/complications , Obesity/genetics , Polymorphism, Genetic , Abdomen , Adipose Tissue , Adolescent , Adult , Aged , Aged, 80 and over , Blood Glucose/metabolism , Body Composition , C-Peptide/metabolism , Cohort Studies , Female , Genotype , Glucose Tolerance Test , Humans , Hypoglycemic Agents/blood , Insulin/blood , Male , Middle Aged , Pedigree , Phenotype , Quebec , Resistin
16.
Mol Psychiatry ; 9(2): 213-8, 2004 Feb.
Article in English | MEDLINE | ID: mdl-14699422

ABSTRACT

Schizophrenia is a common psychiatric disorder with a complex genetic etiology. To understand the genetic basis of this syndrome in Portuguese Island populations, we performed a genome-wide scan of 29 families with schizophrenia, which identified a single region on 5q31-5q35 with strong linkage (NPL=3.09, P=0.0012 at D5S820). Empirical simulations set a genome-wide threshold of NPL=3.10 for significant linkage. Additional support for this locus in schizophrenia comes from higher-density mapping and mapping of 11 additional families. The combined set of 40 families had a peak NPL=3.28 (P=0.00066) at markers D5S2112-D5S820. These data and previous linkage findings from other investigators provide strong and consistent evidence for this genomic region as a susceptibility locus for schizophrenia. Exploratory analyses of a novel phenotype, psychosis, in families with schizophrenia and bipolar disorder detected evidence for linkage to the same markers as found in schizophrenia (peak NPL=3.03, P=0.0012 at D5S820), suggesting that this locus may be responsible for the psychotic symptoms observed in both diseases. Molecular Psychiatry (2004) 9, 213-218. doi:10.1038/sj.mp.4001418 Published online 30 December 2003


Subject(s)
Bipolar Disorder/genetics , Chromosomes, Human, Pair 5 , Genomics , Schizophrenia/genetics , Azores , Genetic Linkage , Genetic Predisposition to Disease , Humans
17.
Clin Genet ; 61(2): 115-25, 2002 Feb.
Article in English | MEDLINE | ID: mdl-11940086

ABSTRACT

The authors have previously shown that individuals heterozygous for ABCA1 mutations have decreased high density lipoprotein cholesterol, increased triglycerides and an increased frequency of coronary artery disease (CAD), and that single nucleotide polymorphisms (SNPs) in the coding region of the ABCA1 gene significantly impact plasma lipid levels and the severity of CAD in the general population. They have now identified several SNPs in non-coding regions of ABCA1 which may be important for the appropriate regulation of ABCA1 expression (i.e. in the promoter, intron 1 and the 5' untranslated region), and have examined the phenotypic effects of these SNPs in the REGRESS population. Out of 12 SNPs, four were associated with a clinical outcome. A threefold increase in coronary events with an increased family history of CAD was evident for the G-191C variant. Similarly, the C69T SNP was associated with a twofold increase in events. In contrast, the C-17G was associated with a decrease in coronary events and the InsG319 was associated with less atherosclerosis. For all these SNPs, the changes in atherosclerosis and CAD occurred without detectable changes in plasma lipid levels. These data suggest that common variation in non-coding regions of ABCA1 may significantly alter the severity of atherosclerosis, without necessarily influencing plasma lipid levels.


Subject(s)
ATP-Binding Cassette Transporters/genetics , Coronary Artery Disease/genetics , Lipids/blood , Mutation , 5' Untranslated Regions , ATP Binding Cassette Transporter 1 , Cohort Studies , Gene Expression Regulation , Humans , Male , Models, Genetic , Phenotype , Polymorphism, Restriction Fragment Length , Polymorphism, Single Nucleotide , Promoter Regions, Genetic , Random Allocation , Time Factors
18.
FEBS Lett ; 509(1): 101-5, 2001 Nov 30.
Article in English | MEDLINE | ID: mdl-11734214

ABSTRACT

Iron-mediated oxidative stress has been implicated in the pathology of the neurodegenerative disease Friedreich ataxia (FRDA). Here, we show that normal upregulation of the stress defense protein manganese superoxide dismutase (MnSOD) fails to occur in FRDA fibroblasts exposed to iron. This impaired induction was observed at iron levels in which increased activation of the redox-sensitive factor NF-kappaB was absent. Furthermore, MnSOD induction could only be partially suppressed by antioxidants. We conclude that an NF-kappaB-independent pathway that may not require free radical signaling is responsible for the reduction of MnSOD induction. This impairment could constitute both a novel defense mechanism against iron-mediated oxidative stress in cells with mitochondrial iron overload and conversely, an alternative source of free radicals that could contribute to the disease pathology.


Subject(s)
Friedreich Ataxia/metabolism , Iron/metabolism , Superoxide Dismutase/metabolism , Antioxidants/pharmacology , Apoptosis , Blotting, Western , Cell Nucleus/metabolism , Cells, Cultured , DNA, Complementary/metabolism , Dose-Response Relationship, Drug , Fibroblasts/metabolism , Free Radical Scavengers/pharmacology , Free Radicals/metabolism , Humans , Mitochondria/metabolism , NF-kappa B/metabolism , Nucleic Acid Hybridization , Oxidation-Reduction , Oxidative Stress , Phenotype , RNA/metabolism , Time Factors , Transcription, Genetic , Up-Regulation
19.
Gut ; 49(6): 773-6, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11709510

ABSTRACT

BACKGROUND: Linkage data have now identified several inflammatory bowel disease (IBD) susceptibility loci but these data have not been consistently replicated in independent studies. One potential explanation for this is the possibility that patients enrolled in such studies may have been erroneously classified with respect to their diagnosis. AIMS: To determine the rate and type of misclassification in a large population of individuals referred for participation in an IBD genetics study and to examine the effect of diagnostic misclassification on the power to detect linkage. METHODS: The medical records of 1096 patients entered into an IBD genetics programme were reviewed using standardised diagnostic criteria. The original patient reported diagnoses were changed, if necessary, based on review, and the reasons for the change in diagnosis were recorded. To evaluate the effect of misclassification on linkage results, simulations were created with Gensim and analysed using Genehunter to evaluate a model for IBD inheritance. RESULTS: Sixty eight of 1096 (6.2%) individuals had a change in diagnosis from that originally reported. The majority of changes were patients with either Crohn's disease or ulcerative colitis who were determined not to have IBD at all. The principal reasons for changes to the original diagnosis were discordance between the patients' subjective reports of diagnosis and actual clinical history, endoscopic, or pathological results; a change in disease pattern over time; and insufficient information available to confirm the original diagnosis. A 10% misclassification rate resulted in 28.4% and 40.2% loss of power to detect a true linkage when using a statistical model for a presumed IBD locus with lambda(s) values of 1.8 and 1.3, respectively. CONCLUSIONS: Diagnostic misclassification occurs in patients enrolled in IBD genetic studies and frequently involves assigning the diagnosis of IBD to non-affected individuals. Even low rates of diagnostic misclassification can lead to significant loss of power to detect a true linkage, particularly for loci with modest effects as are likely to be found in IBD.


Subject(s)
Computer Simulation , Inflammatory Bowel Diseases/diagnosis , Inflammatory Bowel Diseases/genetics , Models, Genetic , Chromosome Mapping , Colitis, Ulcerative/diagnosis , Colitis, Ulcerative/genetics , Crohn Disease/diagnosis , Crohn Disease/genetics , Diagnostic Errors , Genetic Predisposition to Disease , Humans , Lod Score
20.
Psychiatr Serv ; 52(11): 1523-5, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11684751

ABSTRACT

This study examined prescribing practices for antipsychotic medications at 13 Veterans Affairs (VA) medical centers and whether patients' sociodemographic characteristics were associated with receiving novel agents. Automated pharmacy data were used to identify 599 patients who had been diagnosed as having schizophrenia and who had received a prescription for an antipsychotic medication after their last discharge from a VA medical center in 1997. Novel antipsychotics were found to have been prescribed for almost half of the patients (47 percent). In logistic regression analysis, significant variations in prescription of novel agents were found among the facilities and among ethnic groups. The results of this study suggest that prescribing practices are influenced by both facility and patient characteristics.


Subject(s)
Antipsychotic Agents/therapeutic use , Drug Utilization , Hospitals, Veterans , Practice Patterns, Physicians' , Schizophrenia/drug therapy , Adult , Aged , Aged, 80 and over , Female , Humans , Logistic Models , Male , Middle Aged , Odds Ratio , United States , Veterans
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