Subject(s)
Exophthalmos/surgery , Meningeal Neoplasms/pathology , Meningeal Neoplasms/surgery , Meningioma/pathology , Meningioma/surgery , Orbital Neoplasms/surgery , Blindness/diagnosis , Blindness/etiology , Blindness/prevention & control , Diagnosis, Differential , Exophthalmos/etiology , Exophthalmos/pathology , Female , Humans , Meningeal Neoplasms/complications , Meningioma/complications , Middle Aged , Orbital Neoplasms/complications , Orbital Neoplasms/pathology , Treatment OutcomeSubject(s)
Carotid-Cavernous Sinus Fistula/complications , Carotid-Cavernous Sinus Fistula/diagnosis , Diplopia/etiology , Diplopia/prevention & control , Exophthalmos/diagnosis , Exophthalmos/etiology , Aged , Carotid-Cavernous Sinus Fistula/therapy , Diagnosis, Differential , Diplopia/diagnosis , Female , Humans , Treatment OutcomeABSTRACT
The light microscopical demonstration of D-amino acid oxidase (AAOX) activity with cerium (Ce III) as the capturing agent was improved. The incubation medium was stabilized by the employment of triethanolamine and detrane complexed cerium. A considerable increase in intensity of the reaction was accomplished by treatment of the AAOX-incubated sections with Ce III which reacted with the primary reaction product Ce IV-perhydroxide to form Ce IV-hydroxide. In this way the primary reaction product was reduced and enlarged concomitantly. The Ce IV-hydroxide was converted into Ce IV-perhydroxide by H2O2, which was visualized by blue-black stained Ni-DAB complexes. Thus, Ce III is used as capturing agent as well as amplifier (Ce/Ce-H2O2-DAB method). The primary reaction product Ce III-phosphate formed by coreacting phosphatases was selectively extracted by citrate containing glycine-NaOH buffer while Ce IV-perhydroxide remained in the sections. In model experiments it was proven that the perhydroxide groups in the Ce IV-perhydroxide compound initiate predominantly the DAB polymerization while the contribution of Ce III and Ce IV is small.