ABSTRACT
An analytical method using GC-MS/MS combined with quick, easy, cheap, effective, rugged, and safe extraction was developed to determine 57 pesticides in fishery products. The limits of detection and quantification (LOD and LOQ, respectively) of the analytical method ranged between 0.91 and 2.12 ng/g wet mass and 3 and 7 ng/g wet mass, respectively. Moreover, the linearity of the calibration curves was acceptable (R2 > 0.99). The relative pesticide recoveries ranged between 53.87 and 127.2%, and reproducibility ranged between 0.25 and 10.87%. The pesticide residues in brown seaweed, eel, flatfish, shrimp, and abalone samples were analyzed using the developed analytical method, and the results indicate that most samples were not contaminated by the 57 target pesticides, except low levels (< 10 ng/g) of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethene, alachlor, ametryn, isoprothiolane, and prometryn in several samples.
ABSTRACT
In this study, two bacterial species, Salipiger thiooxidans and Exiguobacterium aestuarii, were extracted and screened from the Saemangeum Reservoir. This study examined these species' suitability as a probiotic by confirming the effects of S. thiooxidans and E. aestuarii added to rearing water for L. vannamei. Three experimental groups were evaluated for 6 weeks: (1) a control group reared in natural (i.e., untreated) water (CON), (2) an experimental group in which S. thiooxidans was added to natural water (SMG-A), and (3) natural water inoculated with E. aestuarii (SMG-B). The SMG-B group inoculated with E. aestuarii showed significantly higher final body weight, weight gain, specific growth rates, and feed efficiency than the control group. The SMG-B group inoculated with E. aestuarii exhibited significantly higher levels of serum lysozyme, and ACP and ALP activity than the control and SMG-A groups. The SMG-A and SMG-B groups inoculated with probiotics showed significantly lower total ammonia nitrogen and nitrite than the control group. Our findings suggest that S. thiooxidans and E. aestuarii extracted from the Saemangeum Reservoir can improve the water quality of aquaculture water, and, in particular, E. aestuarii is a potential probiotic for L. vannamei.
ABSTRACT
The spotted halibut is species that has a high potential market value in Korea, but the supply of seed is unstable because of the limited milt production of males. The objective of this research was to explore different aspects, such as CPAs, diluents, dilution ratio, and freezing rates, to develop an optimal sperm cryopreservation. The parameters assessed were movable sperm ratio, sperm activity index, survival rate, and DNA damage. The CPAs tested in this research were propylene glycol, dimethyl sulfoxide (DMSO), methanol, ethylene glycol, and glycerol. Different diluents, including 300 mM sucrose, 300 mM glucose, Stain's solution, and Ringer's solution, were investigated. The previous experiment showed that the optimal CPA for cryopreservation was DMSO with a concentration of 15% with 300 mM as diluent. To determine the effect of the dilution ratio, sperm was diluted to 1:1, 1:2, 1:10, 1:100, and 1:1000 with 300 mM sucrose containing DMSO at a final concentration of 15%. Lastly, the optimal freezing rate of the sperm was evaluated with four different freezing rates (-1, -5, -10, and -20 °C/min). Post-thaw sperm motility was higher with a dilution ratio lower than 1:2, and the freezing rate was less than -5 °C/min. In conclusion, these findings represent the development of a cryopreservation protocol for spotted halibut.
ABSTRACT
This study was conducted to examine floc-harvesting performance by three separation technologies, namely sedimentation, centrifugation, and membrane filtration, for biofloc generated from a BFT system in aquaculture. According to the experimental results, sedimentation demonstrated the poorest harvesting performance with the lowest energy consumption; centrifugation showed the highest harvesting performance with the highest energy burden; membrane filtration achieved better harvesting performance than sedimentation and better energy efficiency than centrifugation. In terms of large-scale floc recovery, a two-step harvesting process utilizing centrifugation with membrane filtration was found to be a reliable way to overcome the limitation of sedimentation and obtain moderate energy-efficiency. Overall, the energy-consuming aspects of the floc-recovery process on an industrial scale should be concerned, even though the use of biofloc as an aquaculture feed would be a positive in terms of an environment-friendly approach to recycling of aquaculture wastewater.
Subject(s)
Aquaculture , WastewaterABSTRACT
The present study was conducted to compare wastewater purification capacities between heterotrophs (BFT) and autotrophs (ABFT) and to evaluate the effects on the growth of fish (Nile tilapia, Oreochromis niloticus) in aquaculture systems. The wastewater treatment capacity of heterotrophs is far superior to that of autotrophs, but the BFT system requires more energy for aquaculture than does the ABFT system. Regardless, both systems effected positive influences on fish growth performance, showing excellent water-purification capacities compared with the control group (CON). No significant differences were found between CON and ABFT or between ABFT and BFT, but there were significant differences between CON and BFT. Both systems BFT and ABFT were revealed to be cost effective in relation to CON, having reduced water replacement by 82%. Therefore, the BFT and ABFT systems could be economical aquaculture systems if due advantage is taken of what both have to offer.