Antimicrobial Efficacy of Allium cepa and Zingiber officinale Against the Milk-Borne Pathogen Listeria monocytogenes.

Listeria monocytogenes is an important food-borne pathogen that causes listeriosis and has a high case fatality rate despite its low incidence. Medicinal plants and their secondary metabolites have been identified as potential antibacterial substances, serving as replacements for synthetic chemical compounds. The present studies emphasize two significant medicinal plants, Allium cepa and Zingiber officinale, and their efficacy against L. monocytogenes. Firstly, a bacterial isolate was obtained from milk and identified through morphology and biochemical reactions. The species of the isolate were further confirmed through 16S rRNA analysis. Furthermore, polar solvents such as methanol and ethanol were used for the extraction of secondary metabolites from A. cepa and Z. officinale. Crude phytochemical components were identified using phytochemical tests, FTIR, and GC-MS. Moreover, the antibacterial activity of the crude extract and its various concentrations were tested against L. monocytogenes. Among all, A. cepa in methanolic extracts showed significant inhibitory activity. Since, the A. cepa for methanolic crude extract was used to perform autography to assess its bactericidal activity. Subsequently, molecular docking was performed to determine the specific compound inhibition. The docking results revealed that four compounds displayed strong binding affinity with the virulence factor Listeriolysin-O of L. monocytogenes. Based on the above results, it can be concluded that the medicinal plant A. cepa has potential antibacterial effects against L. monocytogenes, particularly targeting its virulence.

Diacerein provokes apoptosis, improves redox balance, and downregulates PCNA and TNF-α in a rat model of testosterone-induced benign prostatic hyperplasia: A new non-invasive approach.

One of the most prevalent chronic conditions affecting older men is benign prostatic hyperplasia (BPH), causing severe annoyance and embarrassment to patients. The pathogenesis of BPH has been connected to epithelial proliferation, inflammation, deranged redox balance, and apoptosis. Diacerein (DIA), the anthraquinone derivative, is a non-steroidal anti-inflammatory drug. This study intended to investigate the ameliorative effect of DIA on the prostatic histology in testosterone-induced BPH in rats. BPH was experimentally induced by daily subcutaneous injection of testosterone propionate for four weeks. The treated group received DIA daily for a further two weeks after induction of BPH. Rats' body and prostate weights, serum-free testosterone, dihydrotestosterone, and PSA were evaluated. Prostatic tissue was processed for measuring redox balance and histopathological examination. The BPH group had increased body and prostate weights, serum testosterone, dihydrotestosterone, PSA, and oxidative stress. Histologically, there were marked acinar epithelial and stromal hyperplasia, inflammatory infiltrates, and increased collagen deposition. An immunohistochemical study showed an increase in the inflammatory TNF-α and the proliferative PCNA markers. Treatment with DIA markedly decreased the prostate weight and plasma hormones, improved tissue redox balance, repaired the histological changes, and increased the proapoptotic caspase 3 expression besides the substantial reduction in TNF-α and PCNA expression. In conclusion, our study underscored DIA's potential to alleviate the prostatic hyperplastic and inflammatory changes in BPH through its antioxidant, anti-inflammatory, antiproliferative, and apoptosis-inducing effects, rendering it an effective, innovative treatment for BPH.

Influence of Canning and Storage on Physicochemical Properties, Antioxidant Properties, and Bioactive Compounds of Apricot (Prunus armeniaca L.) Wholes, Halves, and Pulp.

This study aimed to examine the effect of canning and storage on physicochemical, mineral, and antioxidant properties and phenolic composition of apricot wholes, halves, and pulp. The findings for physicochemical properties revealed that the total soluble solids, titratable acidity, total sugars, and ascorbic acid were found higher in apricot pulp (37.15, 1.39, and 20.74% and 7.21 mg/100 g FW, respectively) followed by apricot wholes and halves throughout the storage period. The remarkable contents of potassium, phosphorous, zinc, copper, iron, and manganese were found in the apricot pulp which revealed that canning and storage slightly affected the mineral composition. Bioactive substances were identified and quantified by reversed-phase high-performance liquid chromatography, which indicated a higher presence of chlorogenic acid (34.45 mg/kg FW), quercitin-3-glucoside (16.78 mg/kg FW), neochlorogenic acid (26.52 mg/kg FW), gallic acid (5.37 mg/kg FW), kaempferol (14.22 mg/kg FW), ellagic acid (6.02 mg/kg FW), procyanidin B2 (8.80 mg/kg FW), and epicatechin (9.87 mg/kg FW) in apricot pulp followed by apricot wholes and halves throughout the storage period. The total phenolic content was found highest in apricot pulp (13.76 GAE mg/100 g FW) followed by wholes (8.09 GAE mg/100 g FW) and halves (6.48 GAE mg/100 g FW) which decreased significantly throughout the storage period. Antioxidant properties were assessed by DPPH, ABTS+, MCA, and BCBA, which were found higher in the apricot pulp (92.23 TEAC µg/g DW, 92.33 TEAC µg/g DW, 33.80 TEAC µg/g DW, and 68.40 TEAC µg/g DW, respectively) that is correlated with the higher presence of bioactive compounds. Thus, apricot pulp containing excellent sources of nutrients, minerals, phytochemicals, and antioxidant components could be used for consumption purposes that provide nutraceuticals and antioxidants globally.

Detection of heavy metals, SERS and antibacterial activity of polyvinylpyrolidone modified plasmonic nanoparticles.

Selective and sensitive optical sensor based on surface plasmon resonance for detection of various heavy metals in water using polyvinylpyrolidone modified silver nanoparticles was explained in this present study. The prepared nanoparticles were characterized by UV-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FTIR) and Transmission electron microscopy (TEM). UV-visible spectra show the surface plasmon resonance (SPR) peak at 409 nm corresponding to silver nanoparticles. Crystalline nature of the nanoparticles was evident from TEM images and XRD analysis. TEM images showed average size of 10 nm for prepared silver nanoparticles. FTIR analysis provides the presence of various functional groups responsible for the reduction and stability of the prepared silver nanoparticles. SERS gives the molecular orientation of the adsorbed pyridine molecules via its nitrogen lone pair of electrons on the surface of silver. Prepared polyvinylpyrolidone modified silver nanoparticles (AgNPs) are demonstrated to detect the concentration of heavy metal contaminant Fe3+ ions in water based on linear change in surface plasmon resonance absorption strength. In addition, AgNPs showed promising activity towards E.coli. These observed optical properties suggest the possible utilization of prepared nanoparticles in the application of water purification.

Diclofenac removal from the wastewater using activated sludge and analysis of multidrug resistant bacteria from the sludge.

Diclofenac is an anti-inflammatory drug and has been frequently detected from the wastewater. In the present study, factors affecting diclofenac adsorption on sewage sludge was evaluated. At 1 mg/L initial diclofenac concentration, more than 80% diclofenac removal was achieved. Adsorption increased at higher concentration (100 mg/L concentration) and more than 99% diclofenac was adsorbed from the wastewater. Significant removal of diclofenac was observed after 5 min contact time. The adsorption efficacy was more than 98% after 50 and 60 min. Pseudo-first and second order kinetics revealed reasonable regression value (0.9) indicated that the model is best fitted. Diclofenac adsorption was extremely high at acidic pHs than alkaline range. The sludge samples showed the presence of multi drug resistant bacteria. Vancomycin-resistant enterococcus stains were 27%, Methicillin-resistant Staphylococcus aureus positive strains were 16.5% and Extended-spectrum betal-lactamase-harbouring Enterobacteriacea were 65.4% in the sludge. The drug resistance Enterobacteriaceae revealed 14 Klebsiella pneumonia strains, 11 strains from E. coli and two from the genus Enterobacter. To conclude, the activated sludge could be effectively utilized for the removal of diclofenac from wastewater.

Biocatalytic degradation of organophosphate pesticide from the wastewater and hydrolytic enzyme properties of consortium isolated from the pesticide contaminated water.

The indiscriminate application of various pesticides leads to toxicity to the humans, animals, fishes and threatens the environment and ecosystem. The present study was aimed to investigate pesticide degrading bacteria from the pesticide contaminated sample and to localize organophophate hydrolase activity from the bacteria. Sediment sample was selected as the source of microorganism for the degradation of chlorpyrifos. Enterobacter aerogenes CP2 and Streptococcus pyogenes CP11 isolated from the contaminated sample removed 77 ± 1.8%, 74.2 ± 3.1 chlorpyrifos. These strains have the potential to utilize pesticide as the source of carbon and energy. The pesticides inoculated with both CP 2 and CP 11 enhanced biodegradation of chlorpyrifos at optimized condition. E. aerogenes CP2 and S. pyogenes CP11 produced organophosphate hydrolase activity and localized enzyme biosynthesis. Organophosphate hydrolase activity was high in intracellular, followed by outer membrane and extracellular sample for both bacteria. The treated wastewater has no impact on the seed germination indicated normal cell division, cell elongation and indole-3 acetic acid synthesis. The strain CP2 has the rapid rate of organophosphate degradation among Enterobacter species.