Fluoride leaching from tuff breccia and its removal by natural and commercial adsorbents.

In Japan, the concentration of fluoride (F-) leached from rocks, such as tuff breccia, excavated in tunnel construction projects often exceeds the Japanese environmental standard of 0.8 mg/L. Because of this, proper disposal methods are necessary for managing F--bearing excavated rocks. One effective solution based on circular economy is the use of an adsorption layer system. This system can simultaneously prevent the migration of F- released from excavated rocks and allow the recycling of this construction waste material. To determine the most suitable material for the disposal of excavated F--bearing tuff breccia from a tunnel construction in Hokkaido, Japan, four types of natural geological materials (S-1, S-2, S-3, and S-4) obtained near the tunnel construction site, as well as three types of commercial adsorbents (calcium (Ca), magnesium (Mg), and CaMg adsorbents) were selected for evaluation. The batch adsorption test results showed that S-1 and S-4 had high adsorption capacities for F-, and the adsorption process followed the Langmuir isotherm. The adsorption of F- to the natural adsorbents was strongly influenced by the pH and the presence of bicarbonate ions (HCO3-), but unaffected by chloride (Cl-) and sulfate (SO42-). There was also a strong positive correlation between the abundance of amorphous aluminum (Al) and iron (Fe) extracted and the adsorption of F-, indicating the importance of ion exchange reactions associated with surface OH- in immobilizing F-. Meanwhile, the Mg-bearing adsorbent exhibited the highest adsorption affinity for F- among the commercial adsorbents. This was attributed to adsorption through electrostatic interactions and coprecipitation with magnesium hydroxide (Mg(OH)2) formed during the hydration of magnesium oxide (MgO). To effectively incorporate these adsorbents into the adsorption layer system, parameters such as permeability and residence time need to be determined in order to maximize the retention of F- through adsorption, ion exchange and coprecipitation reactions.

Insights on hazardous metal bioaccessibility, and groundwater impacted by Zn residues from a legacy mine and risk evaluation of adjacent soils.

This study explored the legacy impact of Zinc plant residues (ZPRs) in Kabwe, Zambia, on the environment and human health, particularly in light of the town's reputation for Pb pollution. ZPRs solid samples and groundwater within and around ZPRs zone were collected from the legacy mine, along with soils in a 10 km radius from the mine site. Bioaccessible fractions of Pb and Zn were elucidated by Japanese leaching test (JLT) and simple bioaccessibility extraction test (SBET). Cationic speciation of Pb and Zn from inhalable and ingestible ZPRs particles was investigated via sequential extraction. Groundwater in the ZPRs area showed higher Zn levels (1490 mg/L) compared to Pb (1.7 mg/L). Elevated Zn concentration were facilitated by the presence of soluble Zn sulfates while Pb was constrained due to its precipitation as anglesite. Groundwater sampled outside the ZPRs area was within the Zambia regulatory limits (< 0.5 mg/L for Pb and < 1 mg/L for Zn). Inhalation exposure to < 30 µm dust particles from ZPRs and soils near the mine indicated negligible risk, with < 3% of bioaccessible Pb in artificial lysosomal fluid. Meanwhile, oral intake of ZPRs particles < 250 µm revealed elevated bioaccessible fractions (36% for Pb and 70% for Zn). ZPRs cationic speciation of ingestible particles < 30 µm, 30-75 µm, 75-150 µm and 150-250 µm indicated that the bioaccessible Pb predominantly emanated from labile Pb fractions under gastric conditions with pH < 1. This was due to the dissolution of Pb associated with the exchangeable phase, carbonates and iron/manganese oxides; however, only exchangeable/carbonate Pb was bioaccessible at pH < 2. Hazard quotients indicated increased risks of Pb intoxication through the ingestion of ZPRs and soils near the legacy mine, with higher risks observed in children, emphasizing the need to remediate legacy mine wastes to reduce health risks and protect groundwater through monitoring in mining-affected regions.

Social cost of mining-related lead (Pb) pollution in Kabwe, Zambia, and potential remediation measures.

Lead (Pb) pollution has been one of the major environmental problems of worldwide significance. It is a latent factor for several fatal illnesses, whereas the exposure to lead in early childhood causes a lifetime IQ loss. The social cost is the concept to aggregate various adverse effects in a single monetary unit, which is useful in describing the pollution problem and provides foundation for the design of interventions. However, the assessment of the social cost is scarce for developing countries. In this study, we focus on the lead pollution problem of a former mining town, Kabwe, Zambia, where mining wastes abandoned near residential areas has caused a critical pollution problem. We first investigated the social cost of lead pollution that future generations born in 2025-2049 would incur in their lifetime. As the channels of the social cost, we considered the lost income from the IQ loss and the lost lives from lead-related mortality. The results showed that the social cost would amount to 224-593 million USD (discounted to the present value). Our results can be considered conservative, lower bound estimates because we focused only on well-identified effects of lead, but the social cost was still substantial. Then we examined several engineering remediation measures. The results showed that the social cost can be reduced (the benefits of remediations) more than the costs of implementing remediation measures. This study is the first to investigate the social cost of mining-related lead pollution problem in developing countries. Our interdisciplinary approach utilises the micro-level economic, health and pollution data and integrates the techniques in economics, toxicology and engineering.

Accelerated Bioconversion of Chemically Solubilized Lignite Solution to Methane by Methanogenic Consortium: Experimental Results and Their Application to the Subsurface Cultivation and Gasification Method.

Lignite is an obsolete and less commercially circulated natural resource due to its low calorific value worldwide. The effective conversion of lignite into methane is important considering the global energy crunch. This study reported the effective bioconversion of organic matter released from chemically solubilized lignite to methane using two methanogenic consortia types: mixed methanogenic enrichment culture (mMEC) and SAL25-2. We demonstrated in a microcosm study that the start of methane generation was observed within seven days. Furthermore, the methane yield increased as the total organic carbon concentration of the chemically solubilized lignite solution increased. Surprisingly, methane production using mMEC was drastically enhanced by approximately 50-fold when pulverized lignite was added as conductive material (CM) to the microcosms. To the best of our knowledge, this is the highest number of times methane production increased relative to the control. Our results demonstrated that bioaugmentation using a methanogenic consortium and adding pulverized lignite as CM could facilitate the bioconversion of chemically solubilized lignite solution to methane and lead to effective utilization of subterranean lignite, regarded as a neglected natural resource, without any further excavation processes.

Geochemical audit of a historical tailings storage facility in Japan: Acid mine drainage formation, zinc migration and mitigation strategies.

Historical tailings storage facilities (TSFs) are either abandoned or sparsely rehabilitated promoting acid mine drainage (AMD) formation and heavy metal release. To sustainably manage these sites, a geochemical audit coupled with numerical simulation to predict AMD flow paths and heavy metal migration are valuable. In this study, a 40-year-old TSF in Hokkaido, Japan was investigated. Tailings in this historical TSF contain pyrite (FeS2) while its copper (Cu) and zinc (Zn) contents were 1400-6440 mg/kg and 2800-22,300 mg/kg, respectively. Copper and Zn were also easily released in leaching tests because they are partitioned with the exchangeable phase (29% of Zn; 15% of Cu) and oxidizable fraction (25% of Zn; 33% of Cu). Kinetic modeling results attributed AMD formation to the interactions of pyrite and soluble phases in the tailings with oxygenated groundwater, which is supported by the sequential extraction and leaching results. Calibrations of groundwater/AMD flow and solute transport in the 2D reactive transport model were successfully done using hydraulic heads measured on-site and leaching results, respectively. The model forecasted the quality of AMD to deteriorate with time and AMD formation to continue for 1000 years. It also predicted ~24% AMD flux reduction, including lower Zn release with time when recharge reduction interventions are implemented on-site.

Oosporein Produced by Root Endophytic Chaetomium cupreum Promotes the Growth of Host Plant, Miscanthus sinensis, under Aluminum Stress at the Appropriate Concentration.

Chaetomium cupreum, a root endophyte in Miscanthus sinensis, enhances Al tolerance in M. sinensis by changing aluminum (Al) localization and the production of a siderophore, oosporein, which chelates Al for detoxification. Oosporein has various functions, including insecticidal activity, phytotoxicity, antifungal activity, and a siderophore. In our study, we focused on the detoxification effect of oosporein as a siderophore and on the growth of M. sinensis under Al exposure. In addition, the phytotoxicity of oosporein to M. sinensis was confirmed to compare with those in Lactuca sativa and Oryza sativa as control plants. Under Al stress, oosporein promoted plant growth in M. sinensis seedlings at 10 ppm, which was the same concentration as that detected in M. sinensis roots infected with C. cupreum in our previous study. Oosporein also showed low phytotoxicity to M. sinensis compared with L. sativa at even high concentrations of oosporein. These results suggest that the concentration of oosporein in M. sinensis roots would be maintained at the appropriate concentration to detoxify Al and would promote M. sinensis growth under Al stress, although oosporein would show low phytotoxicity to the natural host plant, M. sinensis, compared with the non-host plant, L. sativa.

Solid-Phase Partitioning and Leaching Behavior of Pb and Zn from Playground Soils in Kabwe, Zambia.

Zambia's Kabwe mine wastes (KMWs) are responsible for contaminating the surrounding soil and dust in the Kabwe district. Unfortunately, these wastes arise from the historical mining activities of lead (Pb) and Zinc (Zn), which lacked adequate waste management strategies. As a result, potentially toxic elements (PTEs) (Pb and Zn) spread across the Kabwe district. To assess the soil pollution derived from previous mining activities, we studied topsoil samples (n = 8) from the school playground soils (SPs). In this study, the degree of contamination, geochemical partitioning, and leachability, coupled with the release and retention of Pb and Zn, were studied. The SPs were classified as extremely enriched (EF > 40) and contaminated with Pb (Igeo > 5). On average, Pb (up to 89%) and Zn (up to 69%) were bound with exchangeable, weak acid-soluble, reducible and oxidizable phases, which are considered as 'geochemically mobile' phases in the environment. The leachates from the soils (n = 5) exceeded the Zambian standard (ZS: 190:2010) for Pb in potable drinking water (Pb < 0.01 mg/L). Furthermore, the spatial distribution of Pb and Zn showed a significant reduction in contents of Pb and Zn with the distance from the mine area.

Biosorption of Pb (II) and Zn (II) from aqueous solution by Oceanobacillus profundus isolated from an abandoned mine.

The present study investigated biosorption of Pb (II) and Zn (II) using a heavy metal tolerant bacterium Oceanobacillus profundus KBZ 3-2 isolated from a contaminated site. The effects of process parameters such as effect on bacterial growth, pH and initial lead ion concentration were studied. The results showed that the maximum removal percentage for Pb (II) was 97% at an initial concentration of 50 mg/L whereas maximum removal percentage for Zn (II) was at 54% at an initial concentration of 2 mg/L obtained at pH 6 and 30 °C. The isolated bacteria were found to sequester both Pb (II) and Zn (II) in the extracellular polymeric substance (EPS). The EPS facilitates ion exchange and metal chelation-complexation by virtue of the existence of ionizable functional groups such as carboxyl, sulfate, and phosphate present in the protein and polysaccharides. Therefore, the use of indigenous bacteria in the remediation of contaminated water is an eco-friendly way of solving anthropogenic contamination.

Solid-phase partitioning and release-retention mechanisms of copper, lead, zinc and arsenic in soils impacted by artisanal and small-scale gold mining (ASGM) activities.

Artisanal and small-scale gold mining (ASGM) operations are major contributors to the Philippines' annual gold (Au) output (at least 60%). Unfortunately, these ASGM activities lacked adequate tailings management strategies, so contamination of the environment is prevalent. In this study, soil contamination with copper (Cu), lead (Pb), zinc (Zn) and arsenic (As) due to ASGM activities in Nabunturan, Davao de Oro, Philippines was investigated. The results showed that ASGM-impacted soils had Cu, Pb, Zn and As up to 3.6, 83, 73 and 68 times higher than background levels, respectively and were classified as 'extremely' polluted (CD = 30-228; PLI = 5.5-34.8). Minerals typically found in porphyry copper-gold ores like pyrite, chalcopyrite, malachite, galena, sphalerite and goethite were identified by XRD and SEM-EDS analyses. Furthermore, sequential extraction results indicate substantial Cu (up to 90%), Pb (up to 50%), Zn (up to 65%) and As (up to 48%) partitioned with strongly adsorbed, weak acid soluble, reducible and oxidisable fractions, which are considered as 'geochemically mobile' phases in the environment. Although very high Pb and Zn were found in ASGM-impacted soils, they were relatively immobile under oxidising conditions around pH 8.5 because of their retention via adsorption to hydrous ferric oxides (HFOs), montmorillonite and kaolinite. In contrast, Cu and As release from the historic ASGM site samples exceeded the environmental limits for Class A and Class C effluents, which could be attributed to the removal of calcite and dolomite by weathering. The enhanced desorption of As at around pH 8.5 also likely contributed to its release from these soils.

Acid mine drainage formation and arsenic mobility under strongly acidic conditions: Importance of soluble phases, iron oxyhydroxides/oxides and nature of oxidation layer on pyrite.

Acid mine drainage (AMD) formation and toxic arsenic (As) pollution are serious environmental problems encountered worldwide. In this study, we investigated the crucial roles played by common secondary mineral phases formed during the natural weathering of pyrite-bearing wastes-soluble salts (melanterite, FeSO4·7H2O) and metal oxides (hematite, Fe2O3)-on AMD formation and As mobility under acidic conditions (pH 1.5-4) prevalent in historic tailings storage facilities, pyrite-bearing rock dumps and AMD-contaminated soils and sediments. Our results using a pyrite-rich natural geological material containing arsenopyrite (FeAsS) showed that melanterite and hematite both directly-by supplying H+ and/or oxidants (Fe3+)-and indirectly-via changes in the nature of oxidation layer formed on pyrite-influenced pyrite oxidation dynamics. Based on SEM-EDS, DRIFT spectroscopy and XPS results, the oxidation layer on pyrite was mainly composed of ferric arsenate and K-Jarosite when melanterite was abundant with/without hematite but changed to Fe-oxyhydroxide/oxide and scorodite when melanterite was low and hematite was present. This study also observed the formation of a mechanically 'strong' coating on pyrite that suppressed the mineral's oxidation. Finally, As mobility under acidic conditions was limited by its precipitation as ferric arsenate, scorodite, or a Fe/Al arsenate phase, including its strong adsorption to Fe-oxyhydroxides/oxides.

Modeling of the groundwater flow system in excavated areas of an abandoned mine.

This study evaluated the assumption that back-filled excavated areas of old mine workings can be modeled as porous media, where groundwater flow is governed by Darcy's law. The Yatani mine, located in Yamagata Prefecture, Japan, was selected for this study because several mining methods were used during its operation and detailed drawings of the excavated areas of the mine are available. The model was calibrated using combinations of hydraulic conductivities (k), with the best-matched case being selected by comparing calculated and measured AMD fluxes. Modeled AMD fluxes along the drainage tunnel (-2 L level) were consistent with measured data when the excavated areas were considered to be porous media with a specific hydraulic conductivity, and the presence of faults and permeability were taken into account. The model also successfully predicted the increasing trend of AMD flux from the shaft to adit mouth. In the numerical model, the back-filled excavated areas were assumed to behave as porous media, which was shown to be a valid assumption in this mine. The model demonstrated that back-filling the excavated areas and drainage tunnel with low permeability materials could reduce the flux of Zn in AMD by up to 61%.

The two-step neutralization ferrite-formation process for sustainable acid mine drainage treatment: Removal of copper, zinc and arsenic, and the influence of coexisting ions on ferritization.

Acid mine drainage (AMD) or acid rock drainage (ARD), the most notorious environmental problem in many mines and underground construction sites, is generally managed using lime neutralization. This approach is effective but unsustainable in the long term, so we introduced the two-step neutralization ferrite-formation process in our previous works as an alternative. However, several important issues related to this new approach-the partitioning of hazardous elements during treatment, stability of generated sludges, and influence of coexisting ions-remains unclear. In this study, real AMD containing zinc (Zn), copper (Cu) and arsenic (As) was treated using a laboratory-type continuous ferrite process flow setup. Partitioning of hazardous elements in the two sludges was elucidated by X-ray fluorescence spectroscopy (XRF) and X-ray absorption spectroscopy (XAS) while the stability of sludges was determined by standard leaching experiments. The bulk of Cu and As species (both As(III) and As(V) based on XANES spectra) were partitioned in the first sludge while ~64% of Zn was associated with the ferrite sludge. In terms of stability, both sludges were relatively inert and released only minute amounts of Zn, Cu and As, all of which were below the Japanese environmental standards. The roles played by two of the most ubiquitous coexisting ions in AMD on ferritization-dissolved silica (Si) and aluminum ion (Al3+)-were also elucidated using 10 synthetic AMDs. Between the two, dissolved Si exhibited stronger adverse effects on ferritization than Al3+. At dissolved Si above 4 mg/L, Si-O-Fe surface complex formation on amorphous Fe-precipitates or Fe-oxide precursor minerals became extensive, which protected these phases from the dissolution-transformation reactions required to form strongly magnetic magnesioferrite and magnetite. These results suggest that the flexibility and applicability of this new AMD treatment approach could be improved by controlling the dissolved Si concentration prior to the ferrite formation step.

Geological and geochemical characterizations of sediments in six borehole cores from the arsenic-contaminated aquifer of the Mekong Delta, Vietnam.

The Mekong Delta, situated between Cambodia and Vietnam, is one of the most productive aquifer systems in the region. In recent years, however, several studies have shown that groundwater in several areas of the delta is highly contaminated with arsenic (As). Although more than 80% of the total area of the Mekong Delta is situated in Vietnam, most of the studies have been conducted on the Cambodian-side of the delta. In this study, borehole core samples were collected around the Tien and Hau Rivers, the two main branches of the Mekong River as it enters Vietnam. We present a raw data collection of the chemical and mineralogical composition of distinct lithological features from six borehole core samples drilled up to a depth of 40 m. The data also include the pH, Eh, EC, As, Si, Al, DOC, dissolved heavy metals (Fe and Mn) and major coexisting ions of leachates obtained by leaching the 34 selected sediment samples in deionized water. The information provided in this paper would be useful as a baseline for reactive transport or geochemical modeling to understand and predict As migration in naturally contaminated aquifers under various conditions. For more insights, the reader is referred to our paper entitled "The solid-phase partitioning of arsenic in unconsolidated sediments of the Mekong Delta, Vietnam and its modes of release under various conditions" Huyen et al., 2019.

Hematite-catalysed scorodite formation as a novel arsenic immobilisation strategy under ambient conditions.

Scorodite is an important mineral not only for arsenic (As) removal from industrial wastewaters but also in the mobility and final fate of As in waste rocks, contaminated soils and sediments, and mine tailings. Because of the mineral's high As-loading capacity and stability, numerous studies have been done to understand its formation. Unfortunately, most of these studies were limited to elevated temperatures (>70 °C), so the processes involved in scorodite formation under ambient conditions remain unclear. This study provides evidence of the catalytic effects of hematite on the formation of scorodite at 25 °C in a pyrite-rich natural geologic material. Scorodite peaks were detected in the XRD patterns of the leaching residues with and without hematite, but those in the former were stronger and more pronounced than the latter. These results suggest that the formation of scorodite was catalysed by hematite, a generalisation that is further supported by strong characteristic IR absorption bands of scorodite at 819 cm-1 (As-O bending vibration), 785 and 725 cm-1 (As-O stretching vibrations), and 2990 cm-1 (OH-vibration) as well as the distinct XPS binding energies of Fe(III)-As (709.7 eV), As(V)-O (44.8, 44.31 and 43.7 eV), O2- (530.5 eV) and coordinated water (531.3 eV) in scorodite. This phenomenon could be attributed to three possible mechanisms: (1) more rapid precipitation promoted by the "seeding" effect of hematite particles, (2) additional supply of Fe3+ from hematite dissolution under acidic conditions, and (3) enhanced oxidations of Fe2+ to Fe3+ and As(III) to As(V) on the surface of hematite.

Acid mine drainage sources and hydrogeochemistry at the Yatani mine, Yamagata, Japan: A geochemical and isotopic study.

This paper describes the geochemistry of groundwater and its flow system in the closed Yatani mine in southern Yamagata Prefecture, Japan. The mine is located in a sulfide deposit containing pyrite and has been generating acid mine drainage (AMD). The study was intended to elucidate the formation of AMD and its flow patterns using geological, hydrological, geochemical, and isotopic techniques. The results indicate that AMD is formed by the interaction of groundwater with sulfide minerals, sand slime, and tailings back-filled into excavated mine areas. Groundwater recharge areas were identified on the mountain slope at an elevation of ~900 m. The formation of AMD in the drifts and shaft was more extensive than that in the deeper drainage levels. Principal component analysis was applied to the hydrogeochemical data to identify the causes of AMD formation. The first, second, and third principal components reveal that the increased ion concentrations in mine drainage are a result of water-mineral reactions in excavated mine areas, the contribution of groundwater in deep reductive environments, and isotopic fractionation during precipitation, respectively. A promising method of reducing AMD formation is to prevent contact between dissolved oxygen and sulfide minerals by increasing the drainage level or by filling the shallow underground excavated area with cementitious materials.

The solid-phase partitioning of arsenic in unconsolidated sediments of the Mekong Delta, Vietnam and its modes of release under various conditions.

Arsenic (As) contamination of the groundwater in the Mekong Delta is a serious problem affecting millions of people who rely on this important resource for drinking and agriculture. In this study, borehole cores up to a depth of 40 m were collected in the Vietnamese-side of the delta, and the solid-phase partitioning of As with depth was investigated to understand the factors and processes controlling the release of this toxic element under oxic, acidic and reducing conditions. The results showed that in most of the sediments, substantial amounts of As are partitioned with exchangeable phases that are easily released into solution. Two borehole cores obtained between the Hau and Tien Rivers also had significantly high As partitioned with organic/sulfide phases and one of these cores had abundant As-bearing pyrite in 1-m thick peat layers. Leaching experiments in deionized (DI) water coupled with principal component analysis suggest that As release was controlled by sorption-desorption reactions with clays/phyllosilicates (i.e., kaolinite, muscovite and clinochlore), proton-promoted dissolution of iron-oxyhydroxides, and oxidation of pyrite/organic matter. The mobility of As was further promoted under acidic conditions in the presence of chloride (Cl-), which suggests that seasonal drying/flooding episodes generating acid sulfate soils, as well as salt water intrusion due to excessive groundwater abstraction may exacerbate this problem in the future.

Efficacy of biocementation of lead mine waste from the Kabwe Mine site evaluated using Pararhodobacter sp.

Biocementation of hazardous waste is used in reducing the mobility of contaminants, but studies on evaluating its efficacy have not been well documented. Therefore, to evaluate the efficacy of this method, physicochemical factors affecting stabilized hazardous products of in situ microbially induced calcium carbonate precipitation (MICP) were determined. The strength and leach resistance were investigated using the bacterium Pararhodobacter sp. Pb-contaminated kiln slag (KS) and leach plant residue (LPR) collected from Kabwe, Zambia, were investigated. Biocemented KS and KS/LPR had leachate Pb concentrations below the detection limit of < 0.001 mg/L, resisted slaking, and had maximum unconfined compressive strengths of 8 MPa for KS and 4 MPa for KS/LPR. Furthermore, biocemented KS and KS/LPR exhibited lower water absorption coefficient values, which could potentially reduce the water transportation of Pb2+. The results of this study show that MICP can reduce Pb2+ mobility in mine wastes. The improved physicochemical properties of the biocemented materials, therefore, indicates that this technique is an effective tool in stabilizing hazardous mine wastes and, consequently, preventing water and soil contamination.

Solidification of sand by Pb(II)-tolerant bacteria for capping mine waste to control metallic dust: Case of the abandoned Kabwe Mine, Zambia.

Environmental impacts resulting from historic lead and zinc mining in Kabwe, Zambia affect human health due to the dust generated from the mine waste that contains lead, a known hazardous pollutant. We employed microbially induced calcium carbonate precipitation (MICP), an alternative capping method, to prevent dust generation and reduce the mobility of contaminants. Pb-resistant Oceanobacillus profundus KBZ 1-3 and O. profundus KBZ 2-5 isolated from Kabwe were used to biocement the sand that would act as a cover to prevent dust and water infiltration. Sand biocemented by KBZ 1-3 and KBZ 2-5 had maximum unconfined compressive strength values of 3.2 MPa and 5.5 MPa, respectively. Additionally, biocemented sand exhibited reduced water permeability values of 9.6 × 10-8 m/s and 8.9 × 10-8 m/s for O. profundus KBZ 1-3 and KBZ 2-5, respectively, which could potentially limit the entrance of water and oxygen into the dump, hence reducing the leaching of heavy metals. We propose that these isolates represent an option for bioremediating contaminated waste by preventing both metallic dust from becoming airborne and rainwater from infiltrating into the waste. O. profundus KBZ 1-3 and O. profundus KBZ 2-5 isolated form Kabwe represent a novel species that has, for the first time, been applied in a bioremediation study.

Arsenic, selenium, boron, lead, cadmium, copper, and zinc in naturally contaminated rocks: A review of their sources, modes of enrichment, mechanisms of release, and mitigation strategies.

Massive and ambitious underground space development projects are being undertaken by many countries around the world to decongest megacities, improve the urban landscapes, upgrade outdated transportation networks, and expand modern railway and road systems. A number of these projects, however, reported that substantial portions of the excavated debris are oftentimes naturally contaminated with hazardous elements, which are readily released in substantial amounts once exposed to the environment. These contaminated excavation debris/spoils/mucks, loosely referred to as "naturally contaminated rocks", contain various hazardous and toxic inorganic elements like arsenic (As), selenium (Se), boron (B), and heavy metals like lead (Pb), cadmium (Cd), copper (Cu), and zinc (Zn). If left untreated, these naturally contaminated rocks could pose very serious problems not only to the surrounding ecosystem but also to people living around the construction and disposal sites. Several incidents of soil and ground/surface water contamination, for example, have been documented due to the false assumption that excavated materials are non-hazardous because they only contain background levels of environmentally regulated elements. Naturally contaminated rocks are hazardous wastes, but they still remain largely unregulated. In fact, standard leaching tests for their evaluation and classification are not yet established. In this review, we summarized all available studies in the literature about the factors and processes crucial in the enrichment, release, and migration of the most commonly encountered hazardous and toxic elements in naturally contaminated geological materials. Although our focus is on naturally contaminated rocks, analogue systems like contaminated soils, sediments, and other hazardous wastes that have been more widely studied will also be discussed. Classification schemes and leaching tests to properly identify and regulate excavated rocks that may potentially pose environmental problems will be examined. Finally, management and mitigation strategies to limit the negative effects of these hazardous wastes are introduced.

Groundwater monitoring of an open-pit limestone quarry: groundwater characteristics, evolution and their connections to rock slopes.

Groundwater flow and its geochemical evolution in mines are important not only in the study of contaminant migration but also in the effective planning of excavation. The effects of groundwater on the stability of rock slopes and other mine constructions especially in limestone quarries are crucial because calcite, the major mineral component of limestone, is moderately soluble in water. In this study, evolution of groundwater in a limestone quarry located in Chichibu city was monitored to understand the geochemical processes occurring within the rock strata of the quarry and changes in the chemistry of groundwater, which suggests zones of deformations that may affect the stability of rock slopes. There are three distinct geological formations in the quarry: limestone layer, interbedded layer of limestone and slaty greenstone, and slaty greenstone layer as basement rock. Although the hydrochemical facies of all groundwater samples were Ca-HCO3 type water, changes in the geochemical properties of groundwater from the three geological formations were observed. In particular, significant changes in the chemical properties of several groundwater samples along the interbedded layer were observed, which could be attributed to the mixing of groundwater from the limestone and slaty greenstone layers. On the rainy day, the concentrations of Ca2+ and HCO3- in the groundwater fluctuated notably, and the groundwater flowing along the interbedded layer was dominated by groundwater from the limestone layer. These suggest that groundwater along the interbedded layer may affect the stability of rock slopes.