Predictors of COVID-19 Vaccine Acceptance among Healthcare Workers in Nigeria.

Healthcare workers (HCWs) are regarded as role models regarding health-related issues, including vaccination. Therefore, it is essential to identify the predictors for COVID-19 vaccine acceptance among them. A cross-sectional study to assess the risk perception, attitudes and knowledge of HCWs toward COVID-19 vaccination was carried out. A total of 710 responses were received between September 2021 and March 2022, from HCWs in the Northern, Western and Eastern regions of Nigeria. Cross tabulations were performed to determine statistical relations between sociodemographic variables, knowledge, attitudes and risk perceptions concerning COVID-19 vaccine acceptance. Multinomial logistic regression analysis was performed to determine the predictive variables for COVID-19 vaccine acceptance. Statistical analyses were performed and P-values less than 0.05 were considered statistically significant at a CI of 95%. Results showed that 59.3% of the participants were amenable to COVID-19 vaccines. Multinomial regression analysis identified 14 variables at α < 0.05 as predictors for vaccine acceptance. Male HCWs were 2.8 times more likely to accept the vaccine than their female counterparts. HCWs that were knowledgeable of the different kinds of vaccines, were willing to recommend the vaccines to their patients, believed that the timing of COVID-19 vaccination was appropriate and had recent vaccination history within three years were 1.6, 24.9, 4.4 and 3.1 times more likely to take COVID-19 vaccine than those not sure. The study found a relatively high trust (51.3%) in the Nigerian Center for Disease Control (NCDC) for information regarding COVID-19 vaccines. Therefore, the NDCD should disseminate more robust insights regarding the safety profiles of various COVID-19 vaccines.

Carbapenemase producing Enterobacteriaceae: Environmental reservoirs as primary targets for control and prevention strategies.

Carbapenemase-producing Enterobacteriaceae (CPE) have become one of the greatest public health challenges globally. In the past decade, antimicrobial resistance (AMR) was viewed as a clinical problem in many parts of the world; hence, the role and magnitude of the contribution of the environment were not well appreciated. This review article was done with online published articles extracted from different databases using search terms related to the work. Evidence has shown that there exists the presence of carbapenemase genes in the environment, consequently fuelling the dissemination with alarming consequences. CPE when acquired causes life-threatening infections in humans. The health and economic impact of these infections are numerous, including treatment failure due to limited therapeutic options which hamper the containment of infectious diseases, further contaminating the environment and worsening the public health challenge. It is a well-known fact that the rate of emergence of resistant genes has outpaced the production of new antimicrobial agents, so it is pertinent to institute effective environmental measures to combat the spread of AMR organisms before it will completely gain a foothold and take us back to 'the pre-antibiotic era'. Environmental sources and reservoirs of resistant genes should therefore be amongst the primary targets for the control and prevention of the spread of resistant genes in the environment. This calls for the effective implementation of the 'one health' strategy with stakeholders committed to the design and enforcement of environmental mitigation policies and guidelines.

Mixed bacterial consortium can hamper the efficient degradation of crude oil hydrocarbons.

Crude oil degradation efficiency can be improved because of co-metabolism that exists when bacterial consortium is applied. However, because of possible vulnerability to environmental conditions and/or antagonistic interactions among members of the consortium, the degradation efficiency can be hampered. In this laboratory-based study, the biodegradation potentials of pure bacterial isolates namely Pseudomonas aeruginosa strain W15 (MW320658), Providencia vermicola strain W8 (MW320661) and Serratia marcescens strain W13 (MW320662) earlier isolated from crude oil-contaminated site and their consortium were evaluated using 3% crude oil-supplemented Bushnell Haas media. The efficiency was evaluated based on the viable cell count, biosurfactant analyses, percentage hydrocarbon degradation using gravimetric analysis and gas chromatography-mass spectrophotometry (GC-MS) analysis. There was decline in the population of W13 and predominance of W15 in the consortium as the incubation period progressed. Accelerated biodegradation of the crude oil hydrocarbons through co-metabolism was not achieved with the consortium; neither was there any improved resilience nor resistance to environmental changes of strain W13. The GC-MS analyses showed that the highest degradation was produced by W15 (48.23%) compared to W8 (46.04%), W13 (45.24%) and the Consortium (28.51%). The biodegradation of the crude oil hydrocarbons by W15, W8, W13 axenic cultures and their consortium treatments demonstrated that the bacterial constituent in a consortium can influence the synergistic effect that improves bioremediation. Future research that focuses on evaluating possible improvement in bioremediation through maintenance of diversity by continuous bioaugmentation using vulnerable but efficient degraders in a consortium is necessary to further understand the application of consortia for bioremediation improvement.

The biology of SARS-CoV-2 and epidemiological analysis of COVID-19 pandemic in Nigeria.

The pandemic triggered by SARS-CoV-2 continuous to pose a global health threat, with cases of new infections and deaths still occurring despite increasing vaccination efforts. As in other regions of the world, the pandemic has led to unprecedented stretch of health and economic systems in countries in Africa. Nigeria, the most populous African country, has not been spared as the number of new infections continues to teeter amid a high level of vaccination hesitancy. Here, we provided a brief look at the background and biology of the virus. We also highlighted the epidemiology of the virus in Nigeria, as well as the challenges and opportunities it presents. Our review will add to the knowledge of SARS-CoV-2 and the situation of the pandemic in Nigeria, and provide a better response to the pandemic and future pandemics.

Population dynamics and crude oil degrading ability of bacterial consortia of isolates from oil-contaminated sites in Nigeria.

Application of bacterial consortium of hydrocarbon degraders to crude oil-contaminated site can enhance bioremediation. This study evaluated the population dynamics and crude oil degradation abilities of various consortia developed from bacterial strains isolated from crude oil-contaminated sites using crude oil-supplemented Bushnell Haas media. Each consortium consisted of three bacterial strains and was designated as Consortium A (Serratia marcescens strain N4, Pseudomonas aeruginosa strain N3R, Pseudomonas aeruginosa strain W11), B (Pseudomonas aeruginosa strain N3R, Pseudomonas aeruginosa strain W11, Pseudomonas protegens strain P7), C (Serratia marcescens strain N4, Pseudomonas aeruginosa strain W11, Pseudomonas protegens strain P7), and D (Pseudomonas aeruginosa strain W15, Providencia vermicola strain W8, Serratia marcescens strain W13). There was progressive decline in the populations of Serratia marcescens strains in the consortia as the incubation period progressed. This may have led to reduction in their synergistic contribution and, subsequently, total degradation ability of crude oil by the consortia. The gravimetric analyses showed that Consortium D produced the highest % crude oil degradation of 29.66% compared to Consortia A, B, and C with 23.73%, 11.86%, and 19.49% respectively. Based on gas chromatography-mass spectrometry analyses, Consortium D produced the highest percentage total petroleum hydrocarbon degradation of 73.65% compared to 68.24%, 68.94%, and 69.19% produced by Consortia A, B, and C respectively. The biodegradation potential of Consortium D also demonstrates the significance of using isolates from the same isolation site in development of consortium for bioremediation.