ABSTRACT
Two stochastic sensors based on the modification of graphite paste with the complexes formed by phthalocyanine (PhCN) with Ni and Cu were designed and used for molecular recognition of IL-8, IL-10, IL-12, and IL-15. The four interleukins were recognized according to their signatures-called toff (qualitative parameter) from the diagrams obtained after measurements. The limit of determination for IL-8 was 1 × 10-4µg mL-1 when both stochastic sensors were used; for IL-10, the determination limit was 4.5 × 10-4µg mL-1 for the Ni complex-based sensor, and 4.5 × 10-7µg mL-1 for the Cu complex-based sensor, respectively; for IL-12, the determination limit was 5 × 10-4µg mL-1 for the Ni complex-based sensor, and 5 × 10-7µg mL-1 for the Cu complex-based sensor, respectively; while for IL-15, the determination limit was 5 × 10-5µg/mL for the Ni complex-based sensor, and 5 × 10-5µg/mL for the Cu complex-based sensor, respectively. The stochastic method used was validated using the following biological fluids: nasal lavage, saliva, serum, and whole blood. Graphical abstract á .