Effects of information provision on willingness to pay for conservation of alpine plants in Japan.

Information provision is essential for obtaining the cooperation of the general public for the conservation of unfamiliar ecosystems towards a sustainable (e.g. carbon-neutral and nature-positive) society. The purpose of this study is to identify effective ways of raising public awareness for ecosystem conservation. We explored the interaction between the manner of information provision (i.e. the medium through which and how much information is provided) and the personal attributes (e.g. environmental attitude) of the recipients on their willingness to pay (WTP) for conservation using Japanese alpine plants as the subject. Discrete choice experiments using an online survey were conducted with public citizens aged 20-69 years across Japan, and data from 8457 respondents were analysed. The data analysis was performed in two steps: 1) estimating individual WTP and 2) exploring factors affecting WTP. The results demonstrated that individual WTP was 135,798 ± 82,840 (mean ± standard deviation) JPY per person for a lifetime. The WTP increased when information was provided in the form of short texts and graphics for those proactive about nature conservation, but increased more when video information was provided to those reactive about nature conservation. The study shows that ecosystem conservation groups need to adapt the amount and format of information for target audiences (e.g. Generation Z youth, who are more sustainability-oriented and prefer to accomplish more in less time).

A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos.

Indole-3-acetic acid (IAA) plays a critical role in many aspects of plant growth and development; however, complete pathways of biosynthesis, localization and many aspects of functions of IAA in rice remain unclear. Here, we report the analysis of a rice tryptophan- (Trp-) and IAA-deficient mutant, tryptophan deficient dwarf1 (tdd1), which is embryonic lethal because of a failure to develop most organs during embryogenesis. Regenerated tdd1 plants showed pleiotropic phenotypes: dwarfing, narrow leaves, short roots and abnormal flowers. TDD1 encodes a protein homologous to anthranilate synthase beta-subunit, which catalyses the first step of the Trp biosynthesis pathway and functions upstream of Trp-dependent IAA biosynthesis. TDD1-uidA and DR5-uidA expression overlapped at many sites in WT plants but was lacking in tdd1, indicating that TDD1 is involved in auxin biosynthesis. Both Trp and IAA levels in flowers and embryos were much lower in tdd1 than in wild type (WT). Trp feeding completely rescued the mutant phenotypes and moderate expression of OsYUCCA1, which encodes a key enzyme in Trp-dependent IAA biosynthesis, also rescued plant height and root length, indicating that the abnormal phenotypes of tdd1 are caused predominantly by Trp and IAA deficiency. In tdd1 embryos, the expression patterns of OSH1 and OsSCR, which mark the presumptive apical region and the L2 layer, respectively, are identical to those in WT, suggesting a possibility either that different IAA levels are required for basic pattern formation than for organ formation or that an orthologous gene compensates for TDD1 deficiency during pattern formation.