ABSTRACT
Radioactivity concentrations of primordial radionuclides Ra-226, Th-232 and K-40 for 31 samples of seven different types of construction materials used in Kütahya Province, Turkey, were analyzed using NaI(Tl) gamma spectroscopy. Ra-226, Th-232 and K-40 activities of all samples ranged between <2.0 and 81.3 ± 1.5, <1.0 and 24.8 ± 0.5 and <7.8 and 819.1 ± 5.5 Bq kg -1, respectively. Radium equivalent activity and Gamma index of sampled building materials were calculated and presented. All results were found to be below the safety limits.
Subject(s)
Radiation Monitoring , Radioactivity , Radium , Construction Materials , Potassium Radioisotopes/analysis , Radiation Monitoring/methods , Radium/analysis , Spectrometry, Gamma/methods , Thorium/analysisABSTRACT
Use of plasma cell-free DNA genomic testing, also know as liquid biopsy, reveals information for early detection and monitoring of solid tumors. Our study reports the analysis of 113 lung and 18 breast cancer patients using commercially available platforms. Lung and breast cancer panel hotspot regions on the genes were investigated. There was a significant increase in isolation efficiency with very fresh blood samples of at least 15 millilitres which were processed in minutes. TP53 gene variations were detected in both types of tumors. Additionally, associations were found for EGFR variations in lung tumors and PIK3CA variations in breast tumors. Mutation assessment of these three genes are recommended as useful biomarkers for predictive studies, to follow up tumor growth and for personalized treatment. Mutations observed in this study warrant further investigation for follow up studies and may justify expression studies. However, in our subsequent studies, we intensify our tumor profiling strategy with other methods. However in terms of true personalized medicine,future plans would include repeating these studies with ctDNA size analysis and methylation analysis of the non-coding region in the individual tumors.
Subject(s)
Breast Neoplasms/diagnosis , Circulating Tumor DNA/blood , Class I Phosphatidylinositol 3-Kinases/genetics , Lung Neoplasms/diagnosis , Tumor Suppressor Protein p53/genetics , Adult , Aged , Aged, 80 and over , Biomarkers, Tumor/genetics , Breast Neoplasms/blood , Breast Neoplasms/genetics , Circulating Tumor DNA/genetics , Early Detection of Cancer , ErbB Receptors/genetics , Female , Genetic Variation , Humans , Liquid Biopsy , Lung Neoplasms/blood , Lung Neoplasms/genetics , Male , Middle AgedABSTRACT
Netrin-1, a laminin-related protein, is known to be involved in the nervous system development. Recently, Netrin-1's involvement in other processes such as cell adhesion, motility, proliferation, and differentiation that are important for the development of epithelial tissues has been described. In addition, Netrin-1 and its receptors, deleted in colorectal cancer and uncoordinated-5 homolog, have been linked to apoptosis and angiogenesis. Since these properties are essential for tumor development, Netrin-1 and its receptors have been reported to promote tumorigenesis in many types of cancers. Here, we review the Netrin-1 mediated regulation of cancer, its potential use as a biomarker, and the targeting of the Netrin-1 pathway to treat cancers.
