ABSTRACT
INTRODUCTION: ARI occupying the first place in the structure of total human morbidity. The aim of the study was to investigate the species diversity of the viruses causing AR among residents of the Novosibirsk region during epidemic season (October to April). MATERIALS AND METHODS: 164 nasopharyngeal swabs were collected and analyzed. Viral RNA/DNA, cDNA synthesis and PCR were carried out employing "RIBO-prep" "eReverta-L", "AmpliSens Influenza virus A/B-FL" and "AmpliSens ARI-screen-FL" kits (CRI of Epidemiology). RESULTS: Etiological agent of the disease was found in 69(43%) samples. Monoinfection was found in 58 (35%). In 14 (9%) samples were detected serogroup I coronaviruses, in 13 (8%) rhinoviruses, in 7 (4%) respiratory syncytial virus, in 6 (4%) parainfluenza virus type 1, in 5 (3%) parainfluenza virus type 3. Adenoviruses and bocavirus were identified in 3 (2%) samples. Parainfluenza virus type 2 and 4, metapneumovirus, serogroup Il coronaviruses (HKU1 and OC43) were presented in 2 (1%) samples. In 11 (7%) samples was found mixed infection. CONCLUSION: The majority of common colds were caused by serogroup I coronaviruses (NL63 and 229E), rhinoviruses and mixed infections. The peak of species variability of viruses caused acute respiratory infections was determined in age group of children 2-4 years old. In older age groups the species variability of analyzed viruses was decreased, rhinovirus infection becomes prevalent.
Subject(s)
Epidemics/statistics & numerical data , Pneumovirus/isolation & purification , Respiratory Tract Infections/virology , Acute Disease , Adolescent , Adult , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Morbidity/trends , Respiratory Tract Infections/epidemiology , Retrospective Studies , Siberia/epidemiology , Young AdultABSTRACT
A system for monitoring and analyzing the effectiveness and efficiency of the performance of the Board of the Federal Inspectorate for the Protection of Consumer Rights and Human Welfare in the Novosibirsk Region was introduced into its activities to estimate the provision of the Novosibirsk Region's population with sanitary epidemiological wellbeing in 2007-2009. The introduction of monitoring was ascertained to increase the effectiveness of budgetary fund surveillance and spending, by predicting the effectiveness and choice of priority lines of activities, by increasing the quality of budgetary services rendered in the provision of sanitary and epidemiological well-being to the population.
Subject(s)
Consumer Behavior/legislation & jurisprudence , Sanitation/standards , Social Welfare/legislation & jurisprudence , Humans , Retrospective Studies , SiberiaSubject(s)
Enterobacteriaceae/isolation & purification , Enterobacteriaceae/pathogenicity , Plants/microbiology , Escherichia coli/genetics , Escherichia coli/isolation & purification , Escherichia coli/pathogenicity , Humans , Morganella morganii/genetics , Morganella morganii/isolation & purification , Morganella morganii/pathogenicity , Solanum tuberosum/microbiology , Yersinia pseudotuberculosis/genetics , Yersinia pseudotuberculosis/isolation & purification , Yersinia pseudotuberculosis/pathogenicityABSTRACT
The chemotactic properties of a number of Azospirillum brasilense natural strains have been studied. Azospirillum demonstrate the positive chemotactic reaction towards the organic acids salts but a poor reaction towards the presence of the attractants like hydrocarbons and aminoacids except for arabinose and glutamic acid. The series of Che- mutants deficient in general chemotaxis has been selected by introducing the transposon Tn5 into the cells of rifampicinresistant mutant strain Azospirillum brasilense 5T-2. The ability of the mutant cells to fast and solid adsorption on the roots of the sterile wheat sprouts is shown to be decreased 2-3 folds as compared with the one of the wild type strain. Chemotaxis is suggested to affect the adsorbtion ability of azospirillum and their associative properties.
Subject(s)
Chemotaxis , Genes, Bacterial , Gram-Negative Aerobic Bacteria/genetics , Symbiosis , Triticum/metabolism , DNA Transposable Elements , Gram-Negative Aerobic Bacteria/physiology , Triticum/microbiologySubject(s)
Anopheles , Bacillus thuringiensis , Culex , Exotoxins , Insecticides , Animals , Larva , Organic Chemicals , Pest Control, Biological , Spores, BacterialABSTRACT
Forty-four patients with acute myocardial infarction were given 0.15 mg/kg propranolol (obsidan) by intravenous drip, after which 80--160 mg of the drug were given daily by mouth for 4--5 days. The extent of the ischemic damage and the dynamics of the infarction zone were judged according to the results of electrocardiotopogram recorded from 35 leads and serial tests for creatine phosphokinase activity in blood serum. The hemodynamic shifts occurring under the effect of the treatment were determined in the same patients. The results were compared with those in a control group (37 patients) identical in clinical and hemodynamic signs. It is established that obsidan given by the schedule proposed reduces significantly the electrocardiographic signs of ischemic damage to the myocardium (according to the ST segment) and the spread of the zone of necrosis in the first days of the follow-up. This is attended by deceleration of the cardiac rhythm and a decrease in systemic arterial pressure and cardiac output. The hemodynamic shifts occur within the ranges of admissible fluctuations and do not cause circulatory insufficiency as a rule.