ABSTRACT
The success rate of spinal fusion surgery is mainly determined by the fixation strength of the spinal bone anchors. This study explores the use of an L-shaped spinal bone anchor that is intended to establish a macro-shape lock with the posterior cortical layer of the vertebral body, thereby increasing the pull-out resistance of the anchor. The performance of this L-shaped anchor was evaluated in lumbar vertebra phantoms (L1-L5) across four distinct perpendicular orientations (lateral, medial, superior, and inferior). During the pull-out experiments, the pull-out force, and the displacement of the anchor with respect to the vertebra was measured which allowed the determination of the maximal pull-out force (mean: 123 N ± 25 N) and the initial pull-out force, the initial force required to start motion of the anchor (mean: 23 N ± 16 N). Notably, the maximum pull-out force was observed when the anchor engaged the cortical bone layer. The results demonstrate the potential benefits of utilising a spinal bone anchor featuring a macro-shape lock with the cortical bone layer to increase the pull-out force. Combining the macro shape-lock fixation method with the conventional pedicle screw shows the potential to significantly enhance the fixation strength of spinal bone anchors.
Subject(s)
Lumbar Vertebrae , Spinal Fusion , Humans , Lumbar Vertebrae/surgery , Spinal Fusion/methods , Spinal Fusion/instrumentation , Pedicle Screws , Biomechanical Phenomena , Suture AnchorsABSTRACT
Wind loading is a primary contributor to structural design costs of concentrating solar-thermal power collectors, such as heliostats and parabolic troughs. These structures must resist the mechanical forces generated by turbulent wind, while the reflector surfaces must maintain optimal optical performance. Studying wind-driven loads at a full-scale, operational concentrating solar-thermal power plant provides insights into the wind impact on the solar collector field beyond the capabilities of wind tunnel tests or state-of-the-art simulations. We conducted comprehensive field measurements of the atmospheric turbulent wind conditions and the resulting structural wind loads on parabolic troughs at the Nevada Solar One plant over a two-year period. The measurement setup included meteorological masts and structural load sensors on four trough rows. Additionally, a lidar scanned the horizontal plane above the trough field. In this study, we describe the high-resolution dataset characterizing the complex flow field and resulting structural loads. This first-of-its-kind dataset will enhance the understanding of wind loading on collector structures and will help in designing the next-generation solar collectors and photovoltaic trackers.
ABSTRACT
Methylene blue (MB) is one of the most widely studied organic molecules in the treatment of wastewater. Sorption, biodegradation, photodegradation, electrochemical oxidation, ozonation, and other advanced oxidative processes are frequently used to remove this dye from water solutions. The unexpected degradation of MB adsorbed on magnetic biochar from aqueous solution was observed. We found that the conditions of handling, such as drying temperature and storage period, substantially influenced the stability of the dye fixed on the (magnetic) carbon adsorbents. Twelve substances were identified by mass spectrometry as products of decomposition, mostly demethylated, oxidated, and hydroxylated substances. The decomposition of MB was further investigated using non-magnetic carbon biochars and aluminosilicate mineral zeolite. Our findings of the spontaneous decomposition of MB and the identification of the species of degradation offer a new approach to evaluate the mechanism of adsorption, the process of regeneration, and the toxicity of treated solutions.
ABSTRACT
A divergent approach to a small library of long-chain 6-amino-1,4,5-triols as novel phytosphingosine-type entities, together with their preliminary cytotoxic evaluation, was achieved. Construction of the target compounds addressed two key aspects. First, the installation of a carbon-nitrogen bond via two prototypes of [3,3]-sigmatropic rearrangements and second the introduction of an alkyl side chain unit by using a late stage olefin cross-metathesis process. As shown in cell viability experiments, the corresponding HCl salts proved to be the most cytotoxic derivatives among all the tested substances, with IC50 values in the lower micromolar range on the Jurkat, HeLa and HCT-116 cell lines.
Subject(s)
Antineoplastic Agents , Antineoplastic Agents/pharmacology , Antineoplastic Agents/chemistry , Sphingosine/chemistryABSTRACT
With the increase in Natural Orifice Transluminal Endoscopic Surgery procedures, there is an increasing demand for surgical instruments with additional degrees of freedom, able to travel along tortuous pathways and guarantee dexterity and high accuracy without compromising the surrounding environment. The implementation of follow-the-leader motion in surgical instruments allows propagating the decided shape through its body and moving through curved paths avoiding sensitive areas. Due to the limited operational area and therefore the instrument size, the steerable shaft of these instruments is usually driven by cables that are externally actuated. However, a large number of degrees of freedom requires a great number of actuators, increasing the system complexity. Therefore, our goal was to design a new memory system able to impose a follow-the-leader motion to the steerable shaft of a medical instrument without using actuators. We present a memory mechanism to control and guide the cable displacements of a cable-driven shaft able to move along a multi-curved path. The memory mechanism is based on a programmable physical track with a mechanical interlocking system. The memory system, called MemoBox, was manufactured as a proof-of-concept demonstration model, measuring 70â mm × 64â mm × 6â mm with 11 programmable elements and featuring a minimum resolution of 1â mm. The prototype shows the ability to generate and shift complex 2D pathways in real-time controlled by the user.
ABSTRACT
Current best-practice aircraft noise calculation models usually apply a so-called lateral attenuation term, i.e., an empirical formula to account for sound propagation phenomena in situations with grazing sound incidence. The recently developed aircraft noise model sonAIR features a physically based sound propagation core that claims to implicitly account for the phenomena condensed in this correction. The current study compares calculations for situations with grazing sound incidence of sonAIR and two best-practice models, AEDT and FLULA2, with measurements. The validation dataset includes on the one hand a large number of commercial aircraft during final approach and on the other hand departures of a jet fighter aircraft, with measurement distances up to 2.8 km. The comparisons show that a lateral attenuation term is justified for best-practice models, resulting in a better agreement with measurements. However, sonAIR yields better results than the two other models, with deviations on the order of only ±1 dB at all measurement locations. A further advantage of a physically based modeling approach, as used in sonAIR, is its ability to account for varying conditions affecting lateral attenuation, like systematic differences in the temperature stratification between day and night or ground cover other than grassland.
ABSTRACT
Tissue transport is a challenge during Minimally Invasive Surgery (MIS) with the current suction-based instruments as the increasing length and miniaturisation of the outer diameter requires a higher pressure. Inspired by the wasp ovipositor, a slender and bendable organ through which eggs can be transported, a flexible transport mechanism for tissue was developed that does not require a pressure gradient. The flexible shaft of the mechanism consists of ring magnets and cables that can translate in a similar manner as the valves in the wasp ovipositor. The designed transport mechanism was able to transport 10wt% gelatine tissue phantoms with the shaft in straight and curved positions and in vertical orientation against gravity. The transport rate can be increased by increasing the rotational velocity of the cam. A rotational velocity of 25 RPM resulted in a transport rate of 0.8 mm/s and increasing the rotation velocity of the cam to 80 RPM increased the transport rate to 2.3 mm/s though the stroke efficiency decreased by increasing the rotational velocity of the cam. The transport performance of the flexible transport mechanism is promising. This means of transportation could in the future be an alternative for tissue transport during MIS.
ABSTRACT
A novel series of proflavine ureas, derivatives 11a-11i, were synthesized on the basis of molecular modeling design studies. The structure of the novel ureas was obtained from the pharmacological model, the parameters of which were determined from studies of the structure-activity relationship of previously prepared proflavine ureas bearing n-alkyl chains. The lipophilicity (LogP) and the changes in the standard entropy (ΔS°) of the urea models, the input parameters of the pharmacological model, were determined using quantum mechanics and cheminformatics. The anticancer activity of the synthesized derivatives was evaluated against NCI-60 human cancer cell lines. The urea derivatives azepyl 11b, phenyl 11c and phenylethyl 11f displayed the highest levels of anticancer activity, although the results were only a slight improvement over the hexyl urea, derivative 11j, which was reported in a previous publication. Several of the novel urea derivatives displayed GI50 values against the HCT-116 cancer cell line, which suggest the cytostatic effect of the compounds azepyl 11b-0.44 µM, phenyl 11c-0.23 µM, phenylethyl 11f-0.35 µM and hexyl 11j-0.36 µM. In contrast, the novel urea derivatives 11b, 11c and 11f exhibited levels of cytotoxicity three orders of magnitude lower than that of hexyl urea 11j or amsacrine.
Subject(s)
Entropy , Proflavine/chemical synthesis , Urea/chemical synthesis , Chemical Phenomena , Fibroblasts/cytology , Fibroblasts/drug effects , Humans , Inhibitory Concentration 50 , Kinetics , Male , Models, Molecular , Proflavine/chemistry , Proflavine/pharmacology , Urea/chemistry , Urea/pharmacologyABSTRACT
A flexible synthetic approach to biologically active sphingoid base-like compounds with a 3-amino-1,2-diol framework was achieved through a [3,3]-sigmatropic rearrangement and late stage olefin cross-metathesis as the key transformations. The stereochemistry of the newly created stereogenic centre was assigned via a single crystal X-ray analysis of the (4S,5R)-5-(hydroxymethyl)-4-vinyloxazolidine-2-thione. In order to rationalise the observed stereoselectivity of the aza-Claisen rearrangement, DFT calculations were carried out. The targeted isomeric sphingoid bases were screened in vitro for anticancer activity on a panel of seven human malignant cell lines. Cell viability experiments revealed that C17-homologues are more active than their C12 congeners.
Subject(s)
Sphingosine/analogs & derivatives , Sphingosine/chemical synthesis , Cell Line, Tumor , Cell Proliferation/drug effects , Cell Survival/drug effects , Crystallography, X-Ray , Drug Screening Assays, Antitumor , Humans , Immunoglobulin G/chemistry , Immunoglobulin G/pharmacology , Melphalan/chemical synthesis , Melphalan/chemistry , Melphalan/pharmacology , Molecular Structure , Sphingosine/chemistry , Sphingosine/pharmacology , Stereoisomerism , Synthetic BiologyABSTRACT
A straightforward synthesis of l-lyxo- and l-xylo-phytosphingosine along with their isomeric analogues has been accomplished. The salient features of this approach are the utilization of [3,3]-sigmatropic rearrangements to install a C-N bond and application of a late stage Wittig or OCM reaction to incorporate the hydrophobic chain unit. The final compounds were evaluated regarding their ability to alter both leukaemia and solid tumor cancer cells viability.
Subject(s)
Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/pharmacology , Sphingosine/analogs & derivatives , Antineoplastic Agents/chemistry , Cell Line, Tumor , Cell Proliferation/drug effects , Chemistry Techniques, Synthetic , Humans , Isomerism , Sphingosine/chemical synthesis , Sphingosine/chemistry , Sphingosine/pharmacologyABSTRACT
Degradation of commercial grade Reactive Black 5 (RB5) azo dye by chemical and electrochemical treatment was examined using a dimensionally stable anode and stainless steel cathodes as electrode materials, with NaCl as supporting electrolyte. The electrochemical treatment was compared to the chemical treatment with hypochlorite generated by electrolysis. The compounds present in the commercial grade RB5 azo dye and the products of its electrochemical degradation were separated using ion-pairing high performance liquid chromatography on reversed phase. The separated species were detected by diode array detector and electrospray ionization mass spectrometry. A suitable ion-pairing reversed phase HPLC-MS method with electrospray ionization for the separation and identification of the components was developed. The accurate mass of the parent and fragment ions were used in the determination of the empirical formulas of the components using the first-order mass spectra. Structural formulas of degradation products were proposed using these information and principles of organic chemistry and electrochemistry.
Subject(s)
Electrolysis/methods , Naphthalenesulfonates/chemistry , Azo Compounds/chemistry , Chromatography, High Pressure Liquid/methods , Coloring Agents/chemistry , Molecular Structure , Molecular Weight , Oxidation-Reduction , Spectrometry, Mass, Electrospray Ionization/methods , Water Purification/methodsSubject(s)
Denture, Partial/adverse effects , Duodenum , Foreign Bodies/diagnosis , Gastrointestinal Hemorrhage/etiology , Pylorus , Stomach Ulcer/etiology , Adult , Foreign Bodies/etiology , Foreign Bodies/therapy , Gastrointestinal Hemorrhage/diagnosis , Gastrointestinal Hemorrhage/therapy , Humans , Male , Stomach Ulcer/diagnosis , Stomach Ulcer/therapyABSTRACT
OBJECTIVES: This work assesses the contribution to climate change resulting from emissions of the group of halogenated greenhouse gases. METHODS: A bottom-up emission model covering 22 technological sectors in four major regions is described. Emission estimates for 1996 and projection for 2010 and 2020 are presented. The costs for deep cuts into projected emission levels are calculated. RESULTS: The substances covered by this study have contributed emissions of 1100 +/- 800 MT CO2 equivalents per year in 1996. In terms of their relative contribution to emissions of CO2 equivalents, this corresponds to 3 +/- 2% of global emissions of all anthropogenic greenhouse gases. The wide range of uncertainty is due to the poorly quantified net global warming potential of the ozone depleting substances, which have an indirect cooling effect on climate through the destruction of stratospheric ozone. For annual emissions of HFCs, PFCs and SF6 (which are regulated under the Kyoto Protocol and for which global warming potentials are well defined), the relative contribution is projected to increase to 2% (600 MT CO2 eq.) of global greenhouse gas emissions by 2010. This trend is expected to continue, emissions are projected to grow to a contribution of roughly 3% (870 MT CO2 eq.) in 2020 compared to 0.9% (300 MT CO2 eq.) in 1996. For HFCs, PFCs and SF6, this study identifies global emission reduction potentials of 260 MT CO2 eq. per year in 2010 and 640 MT CO2 eq. per year in 2020 at below US$ 50 per ton. These values correspond to roughly 40% and 75% of projected emissions in 2010 and 2020, respectively.