Folk Knowledge and Perceptions about the Use of Wild Fruits and Vegetables-Cross-Cultural Knowledge in the Pipli Pahar Reserved Forest of Okara, Pakistan.

Wild fruits and vegetables (WFVs) have been vital to local communities for centuries and make an important contribution to daily life and income. However, traditional knowledge of the use of wild fruits is at risk of being lost due to inadequate documentation. This study aimed to secure this knowledge through intermittent field visits and a semi-structured questionnaire. Using various ethnobotanical data analysis tools and SPSS (IBM 25), this study identified 65 WFV species (52 genera and 29 families). These species, mostly consumed as vegetables (49%) or fruits (43%), were predominantly herbaceous (48%) in wild and semi-wild habitats (67%). 20 WFVs were known to local communities (highest RFC), Phoenix sylvestris stood out as the most utilized species (highest UV). Surprisingly, only 23% of the WFVs were sold at markets. The survey identified 21 unique WFVs that are rarely documented for human consumption in Pakistan (e.g., Ehretia obtusifolia, Euploca strigosa, Brassica juncea, Cleome brachycarpa, Gymnosporia royleana, Cucumis maderaspatanus, Croton bonplandianus, Euphorbia prostrata, Vachellia nilotica, Pongamia pinnata, Grewia asiatica, Malvastrum coromandelianum, Morus serrata, Argemone mexicana, Bambusa vulgaris, Echinochloa colonum, Solanum virginianum, Physalis angulata, Withania somnifera, Zygophyllum creticum, and Peganum harmala), as well as 14 novel uses and five novel edible parts. Despite their ecological importance, the use of WFVs has declined because local people are unaware of their cultural and economic value. Preservation of traditional knowledge through education on conservation and utilization could boost economies and livelihoods in this and similar areas worldwide.

Experiences of family caregivers in dealing with cases of advanced breast cancer: a qualitative study of the sociocultural context in Punjab, Pakistan.

BACKGROUND: Patients with advanced breast cancer require consistent help and support from family caregivers. These caregivers often endure financial burdens and psychological stress, with their experiences significantly influenced by sociocultural factors. This study aims to explore the experiences of family caregivers of advanced breast cancer patients in Punjab province, Pakistan. METHODS: Data was collected through in-depth interviews with fifteen family caregivers of advanced breast cancer patients in three major cities of Punjab, Pakistan. Caregivers, who had been in close contact with the patient for the last two years, were purposively sampled from five major hospitals. The data was analyzed using thematic analysis. RESULTS: The study revealed that the experiences of family caregivers are deeply rooted in the sociocultural context. Key themes identified include social responsibility and cultural reciprocity norms; limited awareness and mobility options for caregivers; financial responsibility and strain; impacts of beauty myths and shyness on caregiving attitudes and decisions; the stressful and emotional nature of caregiving; treatment perspectives influenced by social groups; challenges in consulting male physicians and associated stigma; the role of religious beliefs in caregiving; and stress management, with religion often being a coping mechanism. These factors can contribute to delayed treatment decisions for patients. CONCLUSIONS: Family caregivers are crucial in facilitating timely treatment decisions for advanced breast cancer patients in the Pakistani context. To minimize treatment delays and alleviate caregiver stress, addressing sociocultural barriers in care-seeking is essential. A tailored approach, considering sociocultural and religious factors, is imperative for the management and early diagnosis of breast cancer, necessitating appropriate policymaking and implementation.

Quantification of toxic metals in chicken egg and chicken feed via SOM-artificial neural network.

Poultry products such as meat and eggs are rich sources of proteins, vitamins, and minerals. It is a good indicator of healthy food. Keeping in view, the present study is designed to evaluate the prevalence of toxic heavy metals (lead, nickel, cadmium, and chromium) in chicken eggs and feed. For this purpose, five samples of egg and feed were collected from five different commercial markets in Skardu City. Each sample was prepared using the wet digestion method and analyzed using a flame atomic absorption spectrophotometer. The results showed that lead, nickel, and chromium were present in varying amounts in the feed and egg, with nickel being the most concentrated metal, followed by lead and chromium in egg samples, while the feed samples showed the highest concentration of chromium followed by lead and nickel. However, concentrations of selected heavy metals except cadmium were all above the permissible limit of the World Health Organization. The self-organizing map-artificial neural network is employed for the identification of patterns of heavy metals in chicken feed and egg samples. The lower left neurons of the maps showed higher heavy metal concentrations found in samples taken from Bazar, whereas the rest of the samples showed varied concentrations. A comparison of feed and egg concentrations showed that nickel concentration was lower in feed samples than in egg samples. The lead concentration decreased in eggs except in the Krasmathang feed sample. Chromium concentration presented a negative correlation due to the extremely high concentration found in the Bazar feed sample.

Whole-genome sequencing and variant discovery of Citrus reticulata "Kinnow" from Pakistan.

Citrus is a source of nutritional and medicinal advantages, cultivated worldwide with major groups of sweet oranges, mandarins, grapefruits, kumquats, lemons and limes. Pakistan produces all major citrus groups with mandarin (Citrus reticulata) being the prominent group that includes local commercial cultivars Feutral's Early, Dancy, Honey, and Kinnow. The present study designed to understand the genetic architecture of this unique variety of Citrus reticulata 'Kinnow.' The whole-genome resequencing and variant calling was performed to map the genomic variability that might be responsible for its particular characteristics like taste, seedlessness, juice content, thickness of peel, and shelf-life. A total of 139,436,350 raw sequence reads were generated with 20.9 Gb data in Fastq format having 98% effectiveness and 0.2% base call error rate. Overall, 3,503,033 SNPs, 176,949 MNPs, 323,287 INS, and 333,083 DEL were identified using the GATK4 variant calling pipeline against Citrus clementina. Furthermore, g:Profiler was applied for annotating the newly found variants, harbor genes/transcripts and their involved pathways. A total of 73,864 transcripts harbors 4,336,352 variants, most of the observed variants were predicted in non-coding regions and 1009 transcripts were found well annotated by different databases. Out of total aforementioned transcripts, 588 involved in biological processes, 234 in molecular functions and 167 transcripts in cellular components. In a nutshell, 18,153 high impact variants and 216 genic variants found in the current study, which may be used after its functional validation for marker-assisted breeding programs of "Kinnow" to propagate its valued traits for the improvement of contemporary citrus varieties in the region.

Applying Socioecological Framework to Acid Attack Violence: An Exploration of Inhabitant Factors.

This study focused on the phenomenon of acid attack violence by using the socioecological framework. It explored the personal, relational, institutional, and cultural factors that contribute toward the act of acid violence. By using the qualitative research design, 15 in-depth interviews were conducted from acid victims. Thematic analysis was used to analyze the data. The main themes under the framework of social ecology focused on certain inhabitant aspects such as prevailing poverty, forced marriages, conflicting relationships, lack of social support, the falsified religious interpretation of violence, lack of medical assistance, and poor response of law enforcement agencies. This lack of social support is extended at an institutional level as well as where rehabilitative help and response were absent. This study concluded the complexity and interdependence of all levels factors contribute to the act of acid violence. Moreover, it emphasized the need to develop a biopsychosocial approach to address the issue.

Demand- and supply-side factors associated with the use of contraceptive methods in Pakistan: a comparative study of demographic and health surveys, 1990-2018.

BACKGROUND: A remarkable decline in fertility rates has been observed in many countries, with a primary determinant being an increase in the use of contraceptives. However, the birth rate in Pakistan is still higher compared to the other countries of the region. Therefore, this study aims to assess the effect of demand- and supply-side factors associated with the use of contraceptive measures in Pakistan. METHODS: Secondary data analysis of four data series of the Pakistan Demographic and Health Surveys (PDHS 1990-1991, 2006-2007, 2012-2013 and 2017-2018) were used. The data includes ever-married women aged 15-49 years who had given birth in the previous five years and participated in the family planning module of the PDHS. A total of 25,318 women were included in the analysis. Data were analysed by investigating the associations between independent variables (demand- and supply-side factors) and the use of contraceptive measures through unadjusted odds ratios (OR) and adjusted OR (AOR). RESULTS: The results among demand-side factors indicated that in 2012-2013, women without media exposure were less likely to use contraceptives and the trend remains almost constant for 2017-2018 (AOR = 0.664, 95% CI 0.562-0.784) in 2012-2013 and (AOR = 0.654, 95% CI 0.483-0.885) in 2017-2018. However, they still show a lower likelihood of using contraceptives without media exposure. The results among supply-side factors indicated that absence of transport (2012-2013) and limited visits by family planning workers over the previous 12 months (2006-2007, 2012-2013 and 2017-2018) remained significant factors for not using contraceptive methods. CONCLUSIONS: The results of the study indicate that certain demand- and supply-side factors are associated with the use of contraceptive measures in Pakistan. It highlights the need for the provision of family planning resources and further structural factors, particularly in remote areas.

COVID-19 Pandemic: A Serious Threat for Public Mental Health Globally.

Deep emotion traumas in societies around the globe are overcome by extreme human catastrophes such as natural disasters, social crises, war conflicts and infectious virus induced pandemic diseases, etc., can lead to enormous stress-related disorders. The current ongoing pandemic known as COVID-19 caused by novel Corona virus first appeared in Wuhan, city of China and then rapidly spread in the whole world. It has affected various frontiers of lives and caused numerous psychiatric problems like nervousness, post-traumatic stress disorder (PTSD), fear and uncertainty, panic attacks, depression, obsessive compulsory disorder, xenophobia and racism, etc. Globally COVID-19 has persuaded public mental health crisis. Furthermore, inadequate resources of public mental health services in several countries are discussed in this review, which will be further straighten by the upcoming increase in demand for mental health services due to the COVID-19 pandemic. All mental health sciences including Psychiatry can play a very important role in the comfort of COVID-19 infected individuals and their relatives, healthcare providers and society. We need to learn more about psychological and psychiatric features of COVID-19 from the perceptions of public and global mental health in order to cope up the present deteriorating situation caused by the SARS-CoV-2 pandemic.

Protective effect of gallic acid against arsenic-induced anxiety-/depression- like behaviors and memory impairment in male rats.

The purpose of the present study is to determine the effects of gallic acid (GA) on sodium arsenite (iAS)-induced behavior deficits and memory alteration in male rats. Thirty six animals were divided in to 6 groups (six animals in each) (i) saline+saline; (ii) saline+GA (50 mg/kg); (iii) saline+ GA (100 mg/kg) (iv) iAS + saline; (v) iAS + GA(50 mg/kg); (vi) iAS + GA (100 mg/kg). Animals were treated with iAS (2.5 mg/kg/ml); GA (50 and 100 mg/kg/ml) and saline (0.9%; 1 ml/kg) for 4 weeks. Repeated administration of iAS increases immobility time in forced swim test and decreases time spent in open arm (elevated plus maze) and light box (light dark activity box test) suggests depression like and anxiety-like symptoms respectively. On the other hand, animals treated with iAS + GA decreases immobility time and increases time spent in open arm and light box than saline+iAS treated animals suggests anxiolytic and antidepressant-like behavior of GA. Repeated administration of iAS also involves in memory impairment as observed in the Morris water maze test that is reversed by co-administration of GA, indicates that GA also involves in the enhancement of memory. Brain malondialdehyde (MDA) levels, antioxidant enzymes and acetylcholinesterase (AChE) activities also observed in the present study. Results show that iAS produces oxidative stress by increasing lipid peroxidation and decreasing antioxidant enzyme activity. Conversely co-administration of GA produces antioxidant effects by normalization of oxidative stress induced by iAS. Alteration in iAS induced AChE activity is also reversed by GA. It is suggested that GA via its antioxidant potential, has protective effects on iAS induced behavioral deficits and memory alteration. The findings have a strong implication on iAS induced neurological diseases, such as depression, anxiety, Alzheimer's disease and dementia etc.

Honey as a Potential Natural Antioxidant Medicine: An Insight into Its Molecular Mechanisms of Action.

Honey clasps several medicinal and health effects as a natural food supplement. It has been established as a potential therapeutic antioxidant agent for various biodiverse ailments. Data report that it exhibits strong wound healing, antibacterial, anti-inflammatory, antifungal, antiviral, and antidiabetic effects. It also retains immunomodulatory, estrogenic regulatory, antimutagenic, anticancer, and numerous other vigor effects. Data also show that honey, as a conventional therapy, might be a novel antioxidant to abate many of the diseases directly or indirectly associated with oxidative stress. In this review, these wholesome effects have been thoroughly reviewed to underscore the mode of action of honey exploring various possible mechanisms. Evidence-based research intends that honey acts through a modulatory road of multiple signaling pathways and molecular targets. This road contemplates through various pathways such as induction of caspases in apoptosis; stimulation of TNF-α, IL-1ß, IFN-γ, IFNGR1, and p53; inhibition of cell proliferation and cell cycle arrest; inhibition of lipoprotein oxidation, IL-1, IL-10, COX-2, and LOXs; and modulation of other diverse targets. The review highlights the research done as well as the apertures to be investigated. The literature suggests that honey administered alone or as adjuvant therapy might be a potential natural antioxidant medicinal agent warranting further experimental and clinical research.

Frequency of obsessive compulsive symptoms in depression: A hospital-based cross-sectional study.

OBJECTIVE: To determine the frequency of obsessive compulsive symptoms in patients suffering from depression. METHODS: This cross-sectional study was conducted at the Allama Iqbal Memorial Teaching, Sialkot, Pakistan, from February 2014 to March 2017, and comprised patients suffering from depression. Every patient was examined by a consultant psychiatrist or a senior medical officer in psychiatry, and a psychologist to establish clinical diagnosis of depression according to the tenth revision of the International Statistical Classification of Diseases and Related Health Problems. Adult patients suffering from depression were included. Patients with severe physical illnesses, other psychiatric illnesses, current, past or family history of obsessive compulsive disorder and drug abuse were excluded. Beck Depression Inventory and Yale Brown Obsessive Compulsive Scale were also administered. Data was analysed using SPSS 21. RESULTS: Of the 5,127 patients, 2,318(45.21%) were males and 2,809(54.79%) females. The mean age of the male and female patients was 35.17±12.39 years (range: 18-73 years) and 33.67±13.27 years (range: 18-70 years), respectively. Besides, 1,991(38.83%) patients had moderate depression, 1,647(32.13%) had mild depression and 1,489(29.04%) had severe depression. Moreover, 1,673(32.63%) patients had obsessive compulsive symptoms, including 497(29.70%) patients with mild depression, 599(35.80%) with moderate depression and 577(34.49%) with severe depression. CONCLUSIONS: Around one-third participants had obsessive compulsive symptoms.

Health impact caused by poor water and sanitation in district Abbottabad.

BACKGROUND: Large proportions of people still do not have excess to safe drinking water and proper sanitation. METHODS: Qualitative and quantitative approaches were used to assess the health impacts. Random households were selected. Information was collected from questionnaire through interview schedule method, group discussion and observation checklist. RESULTS: People rated water and sanitation condition in urban as: 10% very good, 27% good, 20% bad, 43% very bad, and none of them said we don't know While in rural areas they rated 10% very good, 36% good, 44% bad, 6% very bad, and 4% of them said we don't know. Water sources in selected urban and rural areas were different. 37% in urban and 68% in rural area depended on bore wells as water source, 22% depended on hand pumps. In urban areas, the disease ratio was typhoid 20%, hepatitis 13%, diarrhoea 27%, skin infection 23%, stomach problems 53% and allergies 33%. In rural areas, after stomach problems, diarrhoea, hepatitis and typhoid ratio was very high as compared to urban area. In rural community, 70% were unaware of poor water and sanitation consequences on health. CONCLUSION: The water and sanitation condition in urban as well as in rural community is poor but in rural community it is even worse The drinking water was contaminated with E. coli, Enterobacter, Salmonella and Clostridium. This observation was correlated with prevalence of many water born diseases especially in rural communities of Abbottabad.