ABSTRACT
This study explored the classification experiences and views of Para Alpine skiers with visual impairment. Data from 11 interviews were analyzed using reflexive thematic analysis to generate three themes: Suitability-The skiers questioned the suitability of the visual measurements, testing environment, and the information they received regarding classification; Exclusivity-Skiers felt certain aspects of the system remain exclusive due to the restrictions of sport classes and lack of the athlete voice; and (Dis)trust-Skiers felt distrust in those implementing the system and in other athletes due to intentional misrepresentation. Speculation surrounding this resulted in the skiers' feeling doubt in their own classification. While there is not a "one size fits all" approach to classification, understanding skiers' experiences can be a vital first step and will help to guide future research into the evolution of this sport's classification.
Subject(s)
Skiing , Humans , Male , Female , Adult , Interviews as Topic , Sports for Persons with Disabilities/classification , Middle Aged , Para-Athletes/classification , Vision Disorders/classification , Athletes/classification , Athletes/psychology , Visually Impaired Persons , Trust , Young AdultABSTRACT
The preservation of labile biomolecules presents a major challenge in chemistry, and deep eutectic solvents (DESs) have emerged as suitable environments for this purpose. However, how the hydration of DESs impacts the behavior of proteins is often neglected. Here, we demonstrate that the amino acid environment and secondary structure of two proteins (bovine serum albumin and lysozyme) and an antibody (immunoglobulin G) in 1:2 choline chloride:glycerol and 1:2 choline chloride:urea follow a re-entrant behavior with solvent hydration. A dome-shaped transition is observed with a folded or partially folded structure at very low (<10 wt % H2O) and high (>40 wt % H2O) DES hydration, while protein unfolding increases between those regimes. Hydration also affects protein conformation and stability, as demonstrated for bovine serum albumin in hydrated 1:2 choline chloride:glycerol. In the neat DES, bovine serum albumin remains partially folded and unexpectedly undergoes unfolding and oligomerization at low water content. At intermediate hydration, the protein begins to refold and gradually retrieves the native monomer-dimer equilibrium. However, ca. 36 wt % H2O is required to recover the native folding fully. The half-denaturation temperature of the protein increases with decreasing hydration, but even the dilute DESs significantly enhance the thermal stability of bovine serum albumin. Also, protein unfolding can be reversed by rehydrating the sample to the high hydration regime, also recovering protein function. This correlation provides a new perspective to understanding protein behavior in hydrated DESs, where quantifying the DES hydration becomes imperative to identifying the folding and stability of proteins.
Subject(s)
Deep Eutectic Solvents , Glycerol , Serum Albumin, Bovine/chemistry , Solvents/chemistry , CholineABSTRACT
While the traditional consensus dictates that high ion concentrations lead to negligible long-range electrostatic interactions, we demonstrate that electrostatic correlations prevail in deep eutectic solvents where intrinsic ion concentrations often surpass 2.5 M. Here we present an investigation of intermicellar interactions in 1:2 choline chloride:glycerol and 1:2 choline bromide:glycerol using small-angle neutron scattering. Our results show that long-range electrostatic repulsions between charged colloidal particles occur in these solvents. Interestingly, micelle morphology and electrostatic interactions are modulated by specific counterion condensation at the micelle interface despite the exceedingly high concentration of the native halide from the solvent. This modulation follows the trends described by the Hofmeister series for specific ion effects. The results are rationalized in terms of predominant ion-ion correlations, which explain the reduction in the effective ionic strength of the continuum and the observed specific ion effects.
ABSTRACT
HYPOTHESIS: The self-assembly of ionic surfactants in deep eutectic solvents has recently been demonstrated, opening up new possibilities in terms of the development of formulated products and templating of nanostructured materials. As it occurs in an aqueous environment, the solvophobic effect drives the formation of micelles in these solvents and specific-ion interactions alter the resulting structures. We hypothesized that the presence of hydrotropic salts would greatly affect the micellar structure in deep eutectic solvents, ultimately leading to the formation of worm-like aggregates. EXPERIMENTS: A systematic investigation performed on hydrotrope-surfactant assemblies in neat and hydrated 1:2 choline chloride:glycerol is presented. The effect of choline salicylate on the micellization of hexadecyltrimethylammonium chloride at different hydrotrope-to-surfactant ratios was probed by contrast variation small-angle neutron scattering. FINDINGS: Here the first investigation on salt-induced micellar growth in deep eutectic solvents is presented. The microscopic characterization of the system shows that the micelle-hydrotrope interaction in pure and hydrated deep eutectic solvents results in a significant increase in micelle elongation. The condensation of the hydrotrope on the micelle, which alters the effective monomer packing, leads to the formation of worm-like micelles with tunable morphology and flexibility. The results presented here present new possibilities in terms of self-assembly and co-assembly in neoteric solvents, where micelle morphology can be controlled through surfactant-salt interactions.
ABSTRACT
The European Spallation Source (ESS), which is under construction in Lund (Sweden), will be the next leading neutron facility with an unprecedented brilliance and novel long-pulse time structure. A long-pulse source not only provides a high time-average flux but also opens the possibility to tune the resolution by using pulse shaping choppers. Thus, an instrument can readily be operated in either a high flux or a high resolution mode. Several of the shorter instruments at the ESS will employ Wavelength Frame Multiplication (WFM) in order to enable a sufficient resolution while offering a continuous and broad wavelength range. A test beamline was operated until the end of 2019 at the research reactor in Berlin to test components and methods, including WFM, in order to prepare the new facility for the operation of neutron instruments and successful first science. We herein demonstrate the implementation of WFM for reflectometry. By selecting a short pulse mode under the same geometrical configuration, we compare and discuss the results for two reference samples. The reported experiments not only serve to prove the reliability of the WFM approach but also, for the first time, demonstrate the full instrument control, data acquisition and data reduction chain that will be implemented at the ESS.
ABSTRACT
We have designed, built, and validated a (quasi)-simultaneous measurement platform called NUrF, which consists of neutron small-angle scattering, UV-visible, fluorescence, and densitometry techniques. In this contribution, we illustrate the concept and benefits of the NUrF setup combined with high-performance liquid chromatography pumps to automate the preparation and measurement of a mixture series of Brij35 nonionic surfactants with perfluorononanoic acid in the presence of a reporter fluorophore (pyrene).
ABSTRACT
Background. This study aimed to evaluate the physiological strain index (PSI) along with specific immune system markers in response to a simulated firefighting occupation workload. Methods. Ten healthy male adults completed a 6-min simulated fire stair climb (SFSC) at 60 steps/min. The protocol consisted of four conditions, some including wearing a 34.04-kg vest to simulate personal protective equipment (PPE) and/or inclusion of a color-word interference test (CWIT) as a distracting mechanism. The PSI was evaluated by continuously monitoring the heart rate and core temperature. Salivary cortisol (CORT) was measured at baseline, mid SFSC and post SFSC. C-reactive protein (CRP) was evaluated at baseline and 1 h post SFSC. Results. Repeated-measures analysis of variance showed a significantly different PSI across conditions (p = 0.001). A significantly elevated PSI was exhibited during all 6 min of SFSC for both PPE and PPE + CWIT conditions. Neither CORT (p = 0.116) nor CRP (p = 0.700) was significantly different across conditions or from baseline. Conclusion. These findings suggest that firefighters are potentially at a substantial degree of physiological stress from exercise and the weight of gear alone. Further work should be conducted to further evaluate the usefulness of the PSI as a means to monitor firefighters during fire suppression.
Subject(s)
Ergonomics , Inflammation , Simulation Training , Stress, Physiological , Firefighters , Humans , Inflammation/blood , Male , Occupational Health , Personal Protective Equipment , Psychology, Applied , Workload , Young AdultABSTRACT
In this work, we present the first example of the self-assembly of phospholipid monolayers at the interface between air and an ionic solvent. Deep eutectic solvents are a novel class of environmentally friendly, non-aqueous, room temperature liquids with tunable properties, that have wide-ranging potential applications and are capable of promoting the self-assembly of surfactant molecules. We use a chemically-consistent Bayesian modelling of X-ray and neutron reflectometry measurements to show that these monolayers broadly behave as they do on water. This method allows for the monolayer structure to be determined, alongside the molecular volumes of the individual monolayer components, without the need for water-specific constraints to be introduced. Furthermore, using this method we are able to better understand the correlations present between parameters in the analytical model. This example of a non-aqueous phospholipid monolayer has important implications for the potential uses of these solvents and for our understanding of how biomolecules behave in the absence of water.
Subject(s)
Lipids/chemistry , Models, Chemical , Solvents/chemistry , Bayes Theorem , Chemistry Techniques, Analytical , Water/chemistryABSTRACT
HYPOTHESIS: Oleosomes are stabilized by a complex outer phospholipid-protein-layer. To improve understanding of its structure and stabilization mechanism, this shell has to be studied in extracellular native conditions. This should be possible by SANS using contrast variation. Oleosomes are expected to be highly temperature stable, with molecular changes occurring first in the protein shell. Direct measurements of changes in the shell structure are also important for processing methods, e.g. encapsulation. EXPERIMENTS: Extracted soybean oleosomes were studied directly and after encapsulation with pectin by SANS using contrast variation. In order to determine structure and size, a shell model of oleosomes was developed. The method was tested against a simple phospholipid-stabilized emulsion. The oleosomes' temperature stability was investigated by performing SANS at elevated temperatures. FINDINGS: Size (Rgâ¯=â¯1380â¯Å) and shell thickness of native and encapsulated oleosomes have been determined. This is the first report measuring the shell thickness of oleosomes directly. For native oleosomes, a shell of 9â¯nm thickness surrounds the oil core, corresponding to a layer of phospholipids and proteins. Up to 90⯰C, no structural change was observed, confirming the oleosomes' high temperature stability. Successful coavervation of oleosomes was shown by an increase in shell thickness of 10â¯nm after electrostatic deposition of pectin.
Subject(s)
Glycine max/chemistry , Lipid Droplets/chemistry , Neutron Diffraction/methods , Emulsions/chemistry , Particle Size , Phospholipids/analysis , Scattering, Small Angle , TemperatureABSTRACT
Deep eutectic solvents have been demonstrated to support amphiphile self-assembly, providing potential alternatives as structure-directing agents in the synthesis of nanostructures, and drug delivery. Here we have expanded on this recent research to investigate the self-assembly of alkyltrimethylammonium bromide surfactants in choline chloride:malonic acid deep eutectic solvent and mixtures of the solvent with water. Surface tension and small-angle neutron scattering were used to determine the behavior of the amphiphiles. Surfactants were found to remain active in the solvent, and surface tension measurements revealed changes in the behavior of the surfactants with different levels of hydration. Small-angle neutron scattering shows that in this solvent the micelle shape depends on the surfactant chain length, varying from globular micelles (aspect ratio â¼2) for short chain surfactants to elongated micelles (aspect ratio â¼14) for long chain surfactants even at low surfactant concentration. We suggest that the formation of elongated micelles can be explained through the interaction of the solvent with the surfactant headgroup, since ion-ion interactions between surfactant headgroups and solvent may modify the morphology of the micelles. The presence of water in the deep eutectic solvents promotes an increase in the charge density at the micelle interface and therefore the formation of less elongated, globular micelles.
ABSTRACT
Little is presently known about the unique nanostructure of deep eutectic solvents (DES). The order of the liquid-solid phase transition is contended and whether DES-water mixtures are merely aqueous solutions, or have properties dominated by the eutectic pair, is unclear. Here, we unambiguously show the structure of choline chloride-malic acid (malicine) as a liquid, and also in solid and hydrated forms, using neutron total scattering on D/H isotope-substituted samples, and quasi-elastic neutron scattering (QENS). Data were refined using empirical potential structure refinement. We show evidence for a stoichiometric complex ion cluster in the disordered liquid, with strong choline-chloride bonding and a hydrogen bond donor (HBD) contribution. The 1:1 eutectic stoichiometry makes these ionic domains more well-defined, with less HBD clustering than seen previously for reline. There is minimal structural difference for the solidified material, demonstrating that this DES solidification is a glass transition rather than a first order phase change. QENS data support this by showing a gradual change in solvent dynamics rather than a step change. The DES structure is mostly retained upon hydration, with water acting both as a secondary smaller HBD at closer range to choline than malic acid, and forming transient wormlike aggregates. This new understanding of DES structure will aid understanding of the properties of these novel green solvents on the molecular length scale in chemical processes, as well as giving an insight into the apparent role of natural DESs in plant physiology.
ABSTRACT
Deep eutectic solvents have shown the ability to promote the self-assembly of surfactants in solution. However, some differences have been found compared with self-assembly in pure water and other polar organic solvents. The behaviour of alkyltrimethylammonium bromides in choline chloride:glycerol deep eutectic solvent has been studied by means of surface tension, X-ray and neutron reflectivity and small-angle neutron scattering. The surfactants were found to remain surface active and showed comparable critical micelle concentrations to the same surfactants in water. Our scattering studies demonstrate that these surfactants form globular micelles with ellipsoidal shape in solution. The size, shape and aggregation number of the aggregates were found to vary with the chain length of the surfactant. Specific solvent-headgroup interactions were not found in this system, unlike those we have previously postulated for anionic surfactants in choline chloride deep eutectic solvents.
ABSTRACT
The air-water interface of three mixtures of partially fluorinated surfactants and hydrocarbon surfactants, C4F9C11H22N(CH3)3Br (fC4hC11TAB) with hexadecyltrimethylammonium bromide (C16TAB), (CF3)2C3F6C10H20N(CH3)3Br (fC5hC10TAB) with C16TAB, and C8F17C6H12N(CH3)3Br (fC8hC6TAB) with C18TAB, have been investigated using surface tension (ST) and neutron reflection (NR). Using the composition of the layer determined by NR, the pseudophase separation model was used to fit the variation of concentration for a specific ST to a free energy of mixing, G(E), that included adjustable quadratic, cubic and quartic terms. In all three cases, G(E) was found to be highly unsymmetrical, being approximately ideal at low surface fractions of hydrocarbon surfactant and repulsive at high fractions with a maximum value of 0.2-0.3RT. The corresponding structure of the layer was also determined by NR and showed that the initial ideal behavior of G(E) probably results from a balance of a gain in energy from a reduced immersion of the fluorocarbon chain, brought about by screening of the fluorocarbon from water by the hydrocarbon surfactant, and a loss from increased fluorocarbon-hydrocarbon repulsion. At higher concentration, there is no space in the layer for further screening and the fluorocarbon-hydrocarbon repulsion leads to the expected positive G(E). The calculated G(E) also indicated that there should be phase separation of the two components in the interface over a bulk composition range of about 60-95% hydrocarbon surfactant. However, experiment indicates no phase separation. It is suggested that there are a number of possible additional negative contributions to G(E) close to a phase transition, which are not possible for a true bulk phase separation, and which prevent surface phase separation unless it is strongly favored.
ABSTRACT
BACKGROUND: Our aim was to evaluate the cost-effectiveness of repeat angioplasty versus new brachiobasilic fistula (BBF) in patients with symptomatic cephalic arch stenosis (CAS). METHODS: Patients presenting with symptomatic CAS (n = 22) underwent angioplasty. They were compared to patients undergoing BBF creation (n = 51). Primary outcomes were functional primary arteriovenous fistulae patency at 3, 6 and 12 months. Data were collected on number of interventions, alternative accesses and hospital days for access-related complications. Quality of life was assessed using Kidney Disease Quality of Life-36 scores. Decision tree, Monte Carlo simulation and sensitivity analysis permitted cost-utility analysis. Healthcare costs were derived from Department of Health figures and are presented as cost (£)/patient/year, cost/access preserved and cost/quality of life-adjusted year (QALY) for each of the treatment strategies. RESULTS: Functional primary patency rates at 3, 6, 12 months were 87.5%, 81% and 43% for repeated angioplasty and 78%, 63% and 41% for BBF. The angioplasty cohort required 1.64 ± 0.23 angioplasties/patient and 0.64 ± 0.34 lines/patient. BBF required 0.36 ± 0.12 angioplasties/patient and 1.2 ± 0.2 lines/patient. Patients in the BBF cohort spent an additional 0.9 days/year in hospital due to access-related complications. Mean cost/patient/year in the angioplasty group was £5247.72/patient/year versus £3807.55/patient/year in the BBF cohort. Mean cost per access saved was £11,544.98 (angioplasty) versus £4979.10 (BBF). Average cost per QALY was £13,809.79 (angioplasty) versus £10,878.72 per QALY (BBF). CONCLUSIONS: CAS poses a difficult management problem with poor outcomes from conventional angioplasty. Optimal management will depend on patient factors, local outcomes and expertise, but consideration should be given to creation of a new BBF as a cost-effective means to manage this difficult problem.
Subject(s)
Angioplasty, Balloon/economics , Arteriovenous Shunt, Surgical/economics , Vascular Diseases/economics , Constriction, Pathologic/economics , Constriction, Pathologic/surgery , Cost-Benefit Analysis , Decision Trees , Humans , Kidney Failure, Chronic/economics , Kidney Failure, Chronic/therapy , Retrospective Studies , Vascular Diseases/surgeryABSTRACT
PURPOSE: Patients with bilateral central vein stenosis present a unique challenge: treatment options are limited, largely unproven and associated with reputedly poor outcomes. Our aim was to compare patency rates of different access and renal replacement treatment (RRT) modalities in patients with bilateral central vein stenosis/occlusion. MATERIAL AND METHODS: Data on all patients presenting to a tertiary referral vascular access centre with end-stage vascular access (defined by bilateral central vein stenosis/occlusion with loss of upper limb access) over a 5-year period were included. 3, 6 and 12-month patencies of translumbar catheters (TLs), tunnelled femoral catheters (Fem), native long saphenous vein loops (SV), prosthetic mid-thigh loop grafts (ThGr), peritoneal dialysis (PD), and expedited donation after cardiac death (DCD) cadaveric renal transplants (Tx) via local allocation policies were compared using log-rank test. Kaplan-Meier survival analysis was used to estimate long-term access survival. RESULTS: One hundred forty-six vascular access modalities were attempted in 62 patients (62 Fem, 25 TL, 15 SV, 25 ThGr, 8 PD, 11 Tx). Median follow-up was 876±57 days. Three, 6 and 12-month primary-assisted patencies for each modality were as follows: Fem: 75.4%, 60% and 28%; TL: 88%, 65% and 50%; SV: 87.5%, 60% and 44.6%; ThGr: 64%, 38% and 23.5%; PD: 62.5%, 62.5% and 50%; Tx: 72.7%, 72.7% and 72.7%. SV had better secondary patency at 900 days (76.9%) than ThGr (49.2%) or Fem (35.8%) (p<0.01). No patients died as a result of loss of access. CONCLUSION: Patients with bilateral central vein stenosis often require more than one vascular access modality to achieve a "personal access solution." Native long saphenous vein loops provided the best long-term patency. Expedited renal transplantation with priority local allocation of DCD organs to patients with precarious vascular access provides a potential solution to this difficult problem.
Subject(s)
Arteriovenous Shunt, Surgical/adverse effects , Blood Vessel Prosthesis Implantation/adverse effects , Catheterization, Central Venous , Graft Occlusion, Vascular/therapy , Kidney Failure, Chronic/therapy , Kidney Transplantation , Peritoneal Dialysis , Renal Dialysis , Saphenous Vein/transplantation , Upper Extremity/blood supply , Adult , Aged , Catheterization, Central Venous/adverse effects , Constriction, Pathologic , Databases, Factual , Graft Occlusion, Vascular/diagnosis , Graft Occlusion, Vascular/etiology , Graft Occlusion, Vascular/physiopathology , Humans , Kaplan-Meier Estimate , Kidney Failure, Chronic/diagnosis , Kidney Transplantation/adverse effects , Male , Middle Aged , Peritoneal Dialysis/adverse effects , Renal Dialysis/adverse effects , Reoperation , Retrospective Studies , Risk Factors , Scotland , Tertiary Care Centers , Time Factors , Treatment Outcome , Vascular Patency , Waiting ListsABSTRACT
PURPOSE: Early cannulation arteriovenous grafts (ecAVGs), such as the GORE Acuseal, have "low bleed" properties permitting cannulation within 24 hours of insertion. They may provide an alternative to tunneled central venous catheters (and associated line complications) in patients requiring urgent vascular access. METHODS: We present our early experience of 37 patients treated with the GORE Acuseal ecAVG. RESULTS: A total of 11 upper limb, 24 lower limb and 2 complex graft procedures were performed. Indications for ecAVG were as follows: bridge to transplantation (21.6%); bridge to arteriovenous fistula (AVF) maturation (8.1%); AVF salvage (8.1%); no native options (67.6%, including 17 patients with bilateral central vein stenosis); 36 AVGs (97.3%) were successfully cannulated. Mean time to first cannulation: 30.4±23.4 hours (range: 2-192). Primary and secondary patency rates at 3, 6 and 12 months were 64.9%, 48.6%, 32.4% and 70.2%, 59.4%, 40.5% respectively. The systemic bacteremia rate was 0.2 per 1,000 access days. There was one perioperative death. Other complications included hematoma at cannulation sites (n=9), pseudoaneurysm (n=3) and local infection at graft site (n=6). A total of 26 of 37 patients (70.6%) achieved a "personal vascular access solution": bridge to transplantation (n=8), bridge to functioning AVF/interposition AVG (n=5), maintenance hemodialysis via ecAVG (n=13); death with functioning AVG (n=1). CONCLUSIONS: Early experience with the GORE Acuseal is encouraging. Patency and bacteremia rates are at least comparable to standard polytetrafluoroethylene grafts. ecAVGs have permitted cannulation within 24 hours of insertion and line avoidance in the majority of patients. Nearly three-quarters of patients achieved a definitive "personal vascular access solution" from their ecAVG.
Subject(s)
Arteriovenous Shunt, Surgical/instrumentation , Blood Vessel Prosthesis Implantation/instrumentation , Blood Vessel Prosthesis , Catheterization , Lower Extremity/blood supply , Upper Extremity/blood supply , Adult , Aged , Arteriovenous Shunt, Surgical/adverse effects , Arteriovenous Shunt, Surgical/mortality , Blood Vessel Prosthesis/adverse effects , Blood Vessel Prosthesis Implantation/adverse effects , Blood Vessel Prosthesis Implantation/mortality , Catheterization/adverse effects , Female , Graft Occlusion, Vascular/etiology , Graft Occlusion, Vascular/physiopathology , Graft Occlusion, Vascular/therapy , Humans , Male , Middle Aged , Prosthesis Design , Prosthesis-Related Infections/microbiology , Prosthesis-Related Infections/therapy , Renal Dialysis , Scotland , Time Factors , Treatment Outcome , Vascular Patency , Young AdultABSTRACT
BACKGROUND: Renal failure is a major cause of morbidity in western Europe, with rising prevalence. Vascular access complications are the leading cause of morbidity among patients on haemodialysis. Considering the health care burden of vascular access failure, there is limited research dedicated to the topic. METHODS: Randomised control trials of medications aimed at improving vascular access patency were identified using a medline search between January 1950 and January 2011. RESULTS: Thirteen randomised trials were identified, investigating antiplatelets, anticoagulants and fish oil in preserving vascular access patency. Outcomes are presented and reviewed in conjunction with the underlying pathophysiological mechanisms of failure of vascular access. DISCUSSION: Vascular access failure is a complex process. Most clinical trials so far have involved medications primarily aimed at preventing thrombosis. Other contributing pathways such as neointimal hyperplasia have not been investigated clinically. Improved outcomes may be seen by linking future therapies to these pathways.
Subject(s)
Arteriovenous Shunt, Surgical , Drug Therapy/trends , Renal Dialysis/methods , Renal Insufficiency/therapy , Vascular Access Devices , Humans , Hyperplasia/prevention & control , Neointima/prevention & control , Platelet Aggregation Inhibitors/therapeutic use , Randomized Controlled Trials as Topic , Retrospective Studies , Treatment OutcomeABSTRACT
PURPOSE: The loss of vision is associated with the impairment of functional ability, including a reduced ability to move around and to utilise all forms of transport. Walking in unfamiliar environments is challenging and driving becomes a legal prohibition. This article explores mobility and access to transport issues of urban and rural dwelling people with vision impairment in Ireland. METHODS: Fourteen focus groups took place consisting of 121 people with vision impairment resident in both urban and rural Ireland. Participant selection was representative of the vision impaired community. Data were recorded, transcribed and analysed using thematic analysis. RESULTS: Poor access to public transport and a perceived absence of public awareness about vision impairment are evident from this study. Customised disability awareness training is deemed essential for both the public and transport staff. Difficulties with access issues within the physical environment are highlighted. CONCLUSION: The lack of availability of accessible transport creates an increased dependency on friends and family, and restricts access to medical, social and rehabilitative services. This study highlights issues concerning inequality of access to appropriate transport for rural vision impaired persons. These issues need to be brought to the attention of social planners and local government.
Subject(s)
Architectural Accessibility/standards , Health Services Accessibility/standards , Mobility Limitation , Transportation/standards , Vision Disorders/rehabilitation , Visually Impaired Persons/rehabilitation , Activities of Daily Living , Adult , Aged , Aged, 80 and over , Attitude of Health Personnel , Female , Focus Groups , Humans , Ireland/epidemiology , Male , Middle Aged , Patient Selection , Rural Population/statistics & numerical data , Severity of Illness Index , Socioeconomic Factors , Urban Population/statistics & numerical data , Vision Disorders/epidemiology , Vision Disorders/physiopathology , Visual AcuityABSTRACT
We show that casein protein micelles--a complex protein/inorganic phosphate structure--can be subjected to high pressures (up to 350 MPa) while making in situ structural measurements using (ultra-)small angle neutron scattering, to give insight to the protein structure, aggregation and stability under pressure.