Anticoccidial efficacy of Garcinia kola (Heckel H.) against experimental Eimeria tenella infection in chicks.

There is a need to advance commercial poultry production to cater to the essential protein needs of an ever-increasing population, however, the rampant occurrence of coccidiosis infection poses a threat to this achievement. This study evaluated the in vivo anticoccidial activities of the extracts and fractions of Garcinia kola against experimental Eimeria tenella infection using broiler chickens as experimental subjects. A total of 40 broiler chicks were experimentally infected with E. tenella and assigned randomly into five groups consisting of eight chicks each. Three days post experimental infection groups I and II were administered orally with tween 80 (0.8%) and Amprolium (30 mg/kg) and served as untreated and treated control groups, respectively whereas Groups III, IV, and V were administered orally with crude methanol extract (CME) at doses of 200, 400 and 600 mg/kg, respectively, for five consecutive days. Daily weight gains were recorded and faecal oocysts per gram (OPG) counts were made by the McMaster Egg counting technique. Blood samples from each experimental group were collected on days 0, 3, 6, and 8 for haematological examination. In the acute toxicity studies, the CME of G. kola did not produce any toxic effect or mortality at doses between 10 and 5000 mg/kg. The CME G. kola was then considered safe and the LD50 was assumed to be > 5000 mg/kg. Graded doses of CME of G. kola considerably (P < 0.05) improved body weight gain and decreased OPG in a dose-depended manner. There was also significant improvement in the Packed Cell Volume (PCV), Red Blood Cell (RBC) and White Blood Cell (WBC) counts upon treatment with the graded doses of CME of G. kola. Besides, G. kola significantly decreased histopathological lesions in the caecum. The results of this study indicates that G. kola may provide beneficial effects against E. tenella-induced coccidiosis in broiler chickens.

Effects of vitamin E administration on Plasmodium berghei induced pathological changes and oxidative stress in mice.

The effects of daily intraperitoneal doses of 1000 i.u/kg body weight of vitamin E on the course of Plasmodium berghei NK 65 infection and the parasite-induced anemia as well as alterations in the relative weight of some selected organs and antioxidant status in mice were investigated. The number of parasitized red cells were not initially affected by the vitamin administration but were persistently lowered after 11th day post infection to the termination of the experiment. The P. berghei infection was found to induce anemia, significantly (P<0.05) increased the relative weight of liver, spleen and kidney but significantly decreased (P<0.05) the relative brain weight. However, all the parasite-induced changes in these parameters were significantly (P<0.05) ameliorated by the vitamin administration. Furthermore, malonydialdehyde concentration in the serum, liver and brain of infected animals was significantly (P<0.05) increased whereas superoxide dismutase and catalase activities were significantly (P<0.05) decreased by the infection. But vitamin E administration was found to, a significant degree (P<0.05), reversed the disease-induced alterations in these oxidative stress markers. It was concluded that vitamin E at the dose and route used prevented P. berghei induced anemia as well as alterations in relative organ weight and antioxidant status in mice.

Effects of vitamin E administration on Plasmodium berghei induced pathological changes and oxidative stress in mice

Abstract. The effects of daily intraperitoneal doses of 1000 i.u/kg body weight of vitamin E on the course of Plasmodium berghei NK 65 infection and the parasite-induced anemia as well as alterations in the relative weight of some selected organs and antioxidant status in mice were investigated. The number of parasitized red cells were not initially affected by the vitamin administration but were persistently lowered after 11th day post infection to the termination of the experiment. The P. berghei infection was found to induce anemia, significantly (P<0.05) increased the relative weight of liver, spleen and kidney but significantly decreased (P<0.05) the relative brain weight. However, all the parasite-induced changes in these parameters were significantly (P<0.05) ameliorated by the vitamin administration. Furthermore, malonydialdehyde concentration in the serum, liver and brain of infected animals was significantly (P<0.05) increased whereas superoxide dismutase and catalase activities were significantly (P<0.05) decreased by the infection. But vitamin E administration was found to, a significant degree (P<0.05), reversed the disease-induced alterations in these oxidative stress markers. It was concluded that vitamin E at the dose and route used prevented P. berghei induced anemia as well as alterations in relative organ weight and antioxidant status in mice