ABSTRACT
OBJECTIVE: To analyze the reference range of thyroid-stimulating hormone (TSH) in different BMI categories and its impact on the classification of hypothyroidism. METHODS: The study included 3,928 individuals free of thyroid disease (without previous thyroid disease, no interfering medications, TSH <10 µUI/mL and thyroid peroxidase antibodies [TPO Abs] <50 IU/mL) who participated in a national, cross-sectional, population-based study and were representative of the adult population of Spain. Data gathered included clinical and demographic characteristics, physical examination, and blood and urine sampling. TSH, free thyroxine, free triiodothyronine, and TPO Ab were analyzed by electrochemiluminescence (E170, Roche Diagnostics, Basel, Switzerland). RESULTS: The reference range (p2.5-97.5) for TSH was estimated as 0.6 to 4.8 µUI/mL in the underweight category (BMI<20 kg/m2 ), 0.6 to 5.5 µUI/mL in the normal-weight category (BMI 20-24.9 kg/m2 ), 0.6 to 5.5 µUI/mL in the overweight category (BMI 25-29.9 kg/m2 ), 0.5 to 5.9 µUI/mL in the obesity category (BMI 30-39.9 kg/m2 ), and 0.7 to 7.5 µUI/mL in the morbid obesity category (BMI ≥40). By using the reference criteria for the normal-weight population, the prevalence of high TSH levels increased threefold in the morbid obesity category (P < 0.01). CONCLUSIONS: Persons with morbid obesity might be inappropriately classified if the standard ranges of normality of TSH for the normal-weight population are applied to them.
Subject(s)
Hypothyroidism/diagnosis , Obesity, Morbid/blood , Observer Variation , Thyrotropin/blood , Adolescent , Adult , Aged , Aged, 80 and over , Autoantibodies/blood , Cross-Sectional Studies , Female , Humans , Hypothyroidism/blood , Male , Middle Aged , Obesity, Morbid/complications , Overweight/blood , Overweight/complications , Prevalence , Reference Values , Spain , Thinness/blood , Thinness/complications , Thyroid Function Tests , Thyroxine/blood , Triiodothyronine/blood , Young AdultABSTRACT
The role of selenium exposure in preventing chronic disease is controversial, especially in selenium-repleted populations. At high concentrations, selenium exposure may increase oxidative stress. Studies evaluating the interaction of genetic variation in genes involved in oxidative stress pathways and selenium are scarce. We evaluated the cross-sectional association of plasma selenium concentrations with oxidative stress levels, measured as oxidized to reduced glutathione ratio (GSSG/GSH), malondialdehyde (MDA), and 8-oxo-7,8-dihydroguanine (8-oxo-dG) in urine, and the interacting role of genetic variation in oxidative stress candidate genes, in a representative sample of 1445 men and women aged 18-85 years from Spain. The geometric mean of plasma selenium levels in the study sample was 84.76 µg/L. In fully adjusted models the geometric mean ratios for oxidative stress biomarker levels comparing the highest to the lowest quintiles of plasma selenium levels were 0.61 (0.50-0.76) for GSSG/GSH, 0.89 (0.79-1.00) for MDA, and 1.06 (0.96-1.18) for 8-oxo-dG. We observed nonlinear dose-responses of selenium exposure and oxidative stress biomarkers, with plasma selenium concentrations above ~110 µg/L being positively associated with 8-oxo-dG, but inversely associated with GSSG/GSH and MDA. In addition, we identified potential risk genotypes associated with increased levels of oxidative stress markers with high selenium levels. Our findings support that high selenium levels increase oxidative stress in some biological processes. More studies are needed to disentangle the complexity of selenium biology and the relevance of potential gene-selenium interactions in relation to health outcomes in human populations.
Subject(s)
Gene-Environment Interaction , Oxidative Stress , Selenium/blood , 8-Hydroxy-2'-Deoxyguanosine , Adolescent , Adult , Aged , Biomarkers/blood , Cross-Sectional Studies , Deoxyguanosine/analogs & derivatives , Deoxyguanosine/urine , Female , Genotype , Glutathione/metabolism , Glutathione Disulfide/urine , Humans , Male , Malondialdehyde/urine , Middle Aged , Spain , Young AdultABSTRACT
We have used two tumor cell clones (B9 and G2), derived from the methylcholanthrene-induced murine fibrosarcoma GR9 and normal BALB/c3T3 fibroblasts, to study the ability of t-BOOH derived reactive oxygen radicals to induce oxidative stress, apoptosis and c-fos and c-jun mRNA transcription. These clones differ in terms of their major histocompatibility complex (MHC) (H-2) class I genes expression, their tumor induction and metastatic potential and their reduced glutathione (GSH) levels. Incubation of both cell clones in the presence of t-BOOH results in the increase of 8-oxo-2'-deoxyguanosine (8-oxo-dG) and malondialdehyde and the decrease of GSH. The xenobiotic also induces the transcription of c-fos and c-jun mRNAs in normal fibroblasts and in B9 cell clone but not in G2 cell clone. In addition, G2 cell clone is more resistant to apoptosis when compared with normal fibroblasts or B9 cell clone. Higher levels of GSH in G2 cell clone may be the reason of their lower transactivation response and apoptosis. Thus lowering GSH concentration may be convenient not only for the efficiency of chemotherapy but also to induce a rather fast and direct apoptosis mechanisms in tumor cells. Most commonly antioxidants tested, superoxide dismutase, catalase, GSH and thiourea, were effective in the inhibition of t-BOOH-induced c-fos and c-jun mRNA transcription in normal fibroblasts suggesting, as expected, that different oxygen species are involved in the observed effects induced by the xenobiotic.