ABSTRACT
Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages, ultimately impairing its normal physiological function. Accumulating evidence underscores the potential of targeted modulation of mechanical cues to enhance skin regeneration, promoting scarless repair by influencing the extracellular microenvironment and driving the phenotypic transitions. The field of skin repair and skin appendage regeneration has witnessed remarkable advancements in the utilization of biomaterials with distinct physical properties. However, a comprehensive understanding of the underlying mechanisms remains somewhat elusive, limiting the broader application of these innovations. In this review, we present two promising biomaterial-based mechanical approaches aimed at bolstering the regenerative capacity of compromised skin. The first approach involves leveraging biomaterials with specific biophysical properties to create an optimal scarless environment that supports cellular activities essential for regeneration. The second approach centers on harnessing mechanical forces exerted by biomaterials to enhance cellular plasticity, facilitating efficient cellular reprogramming and, consequently, promoting the regeneration of skin appendages. In summary, the manipulation of mechanical cues using biomaterial-based strategies holds significant promise as a supplementary approach for achieving scarless wound healing, coupled with the restoration of multiple skin appendage functions.
Subject(s)
Biocompatible Materials , Wound Healing , Humans , Wound Healing/physiology , Biocompatible Materials/therapeutic use , Biocompatible Materials/chemistry , Skin/injuries , Cicatrix/pathologyABSTRACT
BACKGROUND: Human amniotic epithelial cells (hAECs) are seed cells used to treat acute myocardial infarction (AMI), but their mechanism remains unclear. METHODS: We cultured hAECs and extracted exosomes from culture supernatants. Next, we established a stable AMI model in rats and treated them with hAECs, exosomes, or PBS. We assess cardiac function after treatment by echocardiography. Additionally, heart tissues were collected and analyzed by Masson's trichrome staining. We conducted the tube formation and apoptosis assays to explore the potential mechanisms. RESULTS: Cardiac function was improved, and tissue fibrosis was decreased following implantation of hAECs and their exosomes. Echocardiography showed that the EF and FS were lower in the control group than in the hAEC and exosome groups, and that the LVEDD and LVESD were higher in the control group (P<0.05). Masson's trichrome staining showed that the fibrotic area was larger in the control group. Tube formation was more efficient in the hAEC and exosome groups (P<0.0001). Additionally, the apoptosis rates of myocardial cells in the hAEC and exosome groups were significantly decreased (P<0.0001). CONCLUSIONS: hAECs and their exosomes improved the cardiac function of rats after AMI by promoting angiogenesis and reducing the apoptosis of cardiac myocytes.
Subject(s)
Amnion/cytology , Epithelial Cells/metabolism , Epithelial Cells/transplantation , Exosomes/transplantation , Heart/physiopathology , Myocardial Infarction/physiopathology , Myocardial Infarction/therapy , Animals , Apoptosis , Cells, Cultured , Disease Models, Animal , Electrocardiography , Exosomes/metabolism , Exosomes/ultrastructure , Fibrosis , Humans , Male , Myocardial Infarction/diagnostic imaging , Myocytes, Cardiac , Rats, Sprague-DawleyABSTRACT
Aims: A meta-analysis was conducted to evaluate the safety and efficacy of novel oral anticoagulants (NOACs) compared with vitamin K antagonists (VKAs) in patients with left ventricular thrombus (LVT). Methods and Results: We searched PubMed, Web of Science, and Cochrane Library for cohort studies comparing the use of VKAs vs. NOACs for the treatment of LVT from the earliest date available to September 30, 2020. The predetermined efficacy and safety outcomes included thromboembolic events, resolution of LVT, clinically significant bleedings, and all-cause death. Fixed-effects model was used to estimate the pooled effects. Publication bias analyses and sensitivity analyses were conducted to check the robustness of results. A total of 6 studies enrolling 837 patients (mean age 60.2 ± 1.6 years; 77.2% were male) were included. We found no significant differences in thromboembolic events [relative risk (RR) 1.69, 95% confidence interval (CI) 0.94-3.06, P 0.08, I2 12.7%], the rate of resolution of thrombus (RR 1.08, 95% CI 0.96-1.21, P 0.21, I2 4.8%), and clinically significant bleedings (RR 0.70, 95% CI 0.37-1.32, P 0.27, I2 0%) between the VKAs and NOACs group. Additionally, no significant difference in all-cause mortality was found between the two groups (RR 1.24, 95% CI 0.79-1.96, P 0.35, I2 0.0%). Sensitivity analyses, using the "1-study removed" method, detected no significant differences. Conclusion: NOACs and VKAs have similar efficacy and safety in treating LVT, prompting the inference that NOACs are the possible alternatives of VKAs in LVT therapy.
ABSTRACT
Mitogen-activated protein kinase 4 (MKK4), a member of the MAP kinase family, play important roles in response to many environmental and cellular stresses in mammals. In this study, three MKK4 subtypes, EcMKK4-1, EcMKK4-2 and EcMKK4-3, were obtained from grouper Epinephelus coioides. The open reading frame (ORF) of EcMKK4s are obtained and the EcMKK4s proteins contain highly conserved domains: a S_TKc domain, a canonical diphosphorylation group and two conserved MKKK ATP binding motifs, Asp-Phe-Gly (DFG) and Ala-Pro-Glu (APE). EcMKK4s could be found both in the cytoplasmic and nuclear. The EcMKK4s mRNA were detected in all E. coioides tissues examined with the different expression levels, and the expression were up-regulated during SGIV (Singapore grouper iridescent virus) or Vibrio alginolyticus infection. EcMKK4 could significantly reduce the activation of AP-1 reporter gene. The results suggested that EcMKK4s might play important roles in pathogen-caused inflammation.
Subject(s)
Fish Diseases/immunology , Fish Proteins/immunology , Gene Expression Regulation/immunology , Iridovirus/immunology , MAP Kinase Kinase 4/immunology , Perciformes/immunology , Vibrio alginolyticus/immunology , Amino Acid Sequence , Animals , Apoptosis/genetics , Apoptosis/immunology , Cell Line , Cloning, Molecular , Fish Diseases/microbiology , Fish Diseases/virology , Fish Proteins/classification , Fish Proteins/genetics , Gene Expression Profiling , Host-Pathogen Interactions/immunology , Iridovirus/physiology , MAP Kinase Kinase 4/classification , MAP Kinase Kinase 4/genetics , Perciformes/microbiology , Perciformes/virology , Phylogeny , RNA, Messenger/genetics , RNA, Messenger/immunology , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Amino Acid , Up-Regulation/immunology , Vibrio alginolyticus/physiologyABSTRACT
Programmed cell death 4 (PDCD4) in mammals, a gene closely associated with apoptosis, is involved in many biological processes, such as cell aging, differentiation, regulation of cell cycle, and inflammatory response. In this study, grouper Epinephelus coioides PDCD4, EcPDCD4-1 and EcPDCD4-2, were obtained. The open reading frame (ORF) of EcPDCD4-1 is 1413 bp encoding 470 amino acids with a molecular mass of 52.39 kDa and a theoretical pI of 5.33. The ORF of EcPDCD4-2 is 1410 bp encoding 469 amino acids with a molecular mass of 52.29 kDa and a theoretical pI of 5.29. Both EcPDCD4-1 and EcPDCD4-2 proteins contain two conserved MA3 domains, and their mRNA were detected in all eight tissues of E. coioides by quantitative real-time PCR (qRT-PCR) with the highest expression in liver. The expressions of two EcPDCD4s were significantly up-regulated after Singapore grouper iridovirus (SGIV) or Vibrio alginolyticus infection. In addition, over-expression of EcPDCD4-1 or EcPDCD4-2 can inhibit the activity of the nuclear factor-κB (NF-κB) and activator protein-1 (AP-1), and regulate SGIV-induced apoptosis. The results demonstrated that EcPDCD4s might play important roles in E. coioides tissues during pathogen-caused inflammation.
Subject(s)
Apoptosis Regulatory Proteins/immunology , Fish Proteins/immunology , Gene Expression Regulation/immunology , Iridovirus/immunology , Perciformes/immunology , Vibrio alginolyticus/immunology , Amino Acid Sequence , Animals , Apoptosis/genetics , Apoptosis/immunology , Apoptosis Regulatory Proteins/chemistry , Apoptosis Regulatory Proteins/genetics , Cloning, Molecular , Fish Diseases/immunology , Fish Diseases/microbiology , Fish Diseases/virology , Fish Proteins/classification , Fish Proteins/genetics , Gene Expression Profiling , Host-Pathogen Interactions/immunology , Iridovirus/physiology , NF-kappa B/genetics , NF-kappa B/immunology , NF-kappa B/metabolism , Perciformes/microbiology , Perciformes/virology , Phylogeny , Protein Conformation , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Amino Acid , Transcription Factor AP-1/genetics , Transcription Factor AP-1/immunology , Transcription Factor AP-1/metabolism , Vibrio alginolyticus/physiologyABSTRACT
Tongsaimai Tablets/Capsules are composed of Lonicerae Japonicae Flos, Angelicae Sinensis Radix, Achyranthis Bidentatae Radix, Codonopsis Radix, Dendrobii Caulis, Astragali Radix, Scrophulariae Radix, and Glycyrrhizae Radix et Rhizoma, and are effective in promoting blood circulation, removing blood stasis, supplementing Qi, and nourishing Yin. It is widely used in the treatment of peripheral vascular diseases. With 40 years of clinical application, it has accumulated substantial research data and application experience. Its good clinical efficacy and pharmacoeconomic benefits in improving the clinical symptoms of peripheral vascular diseases have been confirmed by relevant research. Meanwhile, this drug has also been recommended by many expert consensus, guidelines, and teaching materials, serving as one of the most commonly used Chinese patent medicines in clinical practice. To further improve the understanding of the drug among clinicians and properly guide its clinical medication, the China Association of Chinese Medicine took the lead and organized experts to jointly formulate this expert consensus. Based on the questionnaire survey of clinicians and the systematic review of research literature on Tongsaimai Tablets/Capsules with clinical problems in the PICO framework, the consensus, combined with expert experience, concludes recommendations or consensus suggestions by GRADE system with the optimal evidence available through the nominal group technique. This consensus defines the indications, usage, dosage, course of treatment, medication time, combined medication, and precautions of Tongsaimai Tablets/Capsules in the treatment of peripheral vascular diseases, and explains the safety of its clinical application. It is recommended for clinicians and pharmacists in the peripheral vascular department(vascular surgery), traditional Chinese medicine surgery(general surgery), and endocrinology department of hospitals at all levels in China.
Subject(s)
Drugs, Chinese Herbal , Peripheral Vascular Diseases , Capsules , Consensus , Humans , Medicine, Chinese Traditional , TabletsABSTRACT
The safety of water quantity and quality caused by large-scale blackwater in reservoirs with Eucalyptus plantation is currently a point of great interest. Eucalyptus is largely planted in southern China, especially in Nanning, Guangxi, where more than 90% of the drinking water source reservoirs are surrounded by Eucalyptus, and different degrees of blackwater often occur in many reservoirs. Recent research has demonstrated that reservoir sediments play an important role in the migration and transformation of Fe2+, Mn2+, S2-, and dissolved organic carbon (DOC) in the overlying water. It is of great significance to explore the distribution characteristics and migration rules of pollutants in the sediment-water interface to reveal the mechanism of blackwater in reservoirs. Experiments were carried out three times in a typical blackwater reservoir (Tianbao Reservoir) in southern China from July to December 2018. The distribution characteristics and seasonal variations of iron, manganese, sulfide, and organic matter in sediments were analyzed, focusing mainly on the profile distribution and migration direction of Fe2+, Mn2+, S2- and DOC in pore water during blackwater periods. The results showed that:â The content of iron and manganese in sediments of reservoirs with Eucalyptus plantation is high, far exceeding the background value of soil content in China. The content of iron, manganese, and total organic carbon (TOC) in the surface sediments increases simultaneously, mainly caused by the input and settlement of the material (litter, decomposed liquids. and soil particles) in the Eucalyptus forest around the reservoir. â¡ The concentration of Fe2+(16.99 mg·L-1) and the content of DOC (36.80%) in pore water during the blackwater period are significantly higher than those in Taihu Lake during the black bloom (12.15 mg·L-1, 10.78%). The mean concentrations of Fe2+ and Mn2+ are more than 300 times higher than that of S2-, and the reduction conditions in the sediments are dominated by iron and manganese oxides. ⢠The diffusion flux of Fe2+ is 27.4-33.5 mg·(m2·d)-1, which is 32.6, 4.9, and 30.8 times higher than those of Taihu Lake, Aha Reservoir, and Hongfeng Lake, respectively. This implies strong Fe2+ release ability from sediments to the overlying water. As a positive correction exists between Fe2+ and DOC, the complex reaction between Fe2+ and organic matter is one of the most important causes of blackwater in reservoirs with Eucalyptus plantation.
ABSTRACT
Heat shock protein 22 (Hsp22) is an important regulatory factor response to various stresses in mammals. In this study, the full length cDNA of Epinephelus coioides Hsp22, which was 1680bp in length, with a 289 bp 5' UTR, a 725 bp 3'UTR, and a 666 bp open reading frame encoding 221 amino acids, was obtained. E. coioides Hsp22 contains a highly conserved α-crystallin domain. E. coioides Hsp22 mRNA was detected in all tissues examined by quantitative real-time PCR, with the highest expression in blood, followed by the spleen, skin, gill, head kidney, muscle, heart, liver, trunk kidney, stomach, pyloric caeca, intestine, brain and thymus. The expression patterns of E. coioides Hsp22 response to infection with Singapore grouper iridovirus (SGIV) and Vribro alginolyticus, the important pathogens of E. coioides, were studied. The expression levels of the gene were up-regulated in the tissues examined. Subcellular localization analysis demonstrated that E. coioides Hsp22 was distributed in both the cytoplasm and nucleus. In addition, E. coioides Hsp22 significantly inhibited the SGIV-induced cell apoptosis. In summary, the E. coioides Hsp22 might play a critical role in pathogenic stimulation.
Subject(s)
Bass/immunology , Fish Proteins/genetics , Heat-Shock Proteins/genetics , Vibrio Infections/veterinary , Virus Diseases/veterinary , Animals , Bass/microbiology , Bass/virology , Cloning, Molecular , Fish Diseases/immunology , Fish Diseases/microbiology , Fish Diseases/virology , Gene Expression , Heat-Shock Proteins/immunology , Iridovirus , Vibrio Infections/immunology , Vibrio alginolyticus , Virus Diseases/immunologyABSTRACT
BACKGROUND Research interest in endothelial nitric oxide synthase(eNOS) polymorphisms and atrial fibrillation (AF) has grown in last recent years, but the results of individual studies are inconsistent due to their small sample sizes. MATERIAL AND METHODS We searched databases for eligible studies on eNOS and AF, extracted the relevant data, and rigorously screened them according to inclusion and exclusion criteria. Then, we evaluated the study quality according to the Newcastle-Ottawa scale score, and we pooled the odds ratios (ORs) and 95% confidence intervals (CIs) by using a random-effects model or fixed-effects model based on inter-study heterogeneity. In addition, we performed subgroup analysis and sensitivity analysis and assessed publication bias. RESULTS According to the inclusion and exclusion criteria, we finally found 8 studies in this search. The recessive (OR=0.81; 95% CI=0.67 to 0.97; p=0.988; I²=0.0%) model showed that the eNOS 786T/C polymorphism was relevant to AF. We also found that the eNOS 786T/C polymorphism decreases the risk of AF, especially in white people (OR=0.81; 95% CI=0.67 to 0.97; P=0.023 for recessive model) and in the control population (OR=0.79; 95% CI=0.65 to 0.97; P=0.022 for recessive model). We found no obvious publication bias. CONCLUSIONS The eNOS gene loci 786T/C polymorphism is relevant to the risk of AF. Our results suggest that the 786T/C polymorphism significantly decreases AF risks in white people and control populations. Larger studies are required for further evaluation.
Subject(s)
Atrial Fibrillation/enzymology , Atrial Fibrillation/genetics , Genetic Predisposition to Disease , Nitric Oxide Synthase Type III/genetics , Polymorphism, Single Nucleotide/genetics , Alleles , Case-Control Studies , Humans , Models, Genetic , Publication Bias , Risk FactorsABSTRACT
The issue that genetic polymorphism of tumor necrosis factor-α (TNF-α) is associated with dilated cardiomyopathy (DCM) is debatable. We sought to investigate the potential role of TNF-α gene polymorphism (G-308A) in the susceptibility to dilated cardiomyopathy.We retrieved PubMed, EMBASE, and CNKI to collect all articles which reported on the association between TNF-α G-308A polymorphism and dilated cardiomyopathy. Two authors used the Newcastle-Ottawa Scale (NOS) checklist to assess the quality of the included studies. The odds ratio (OR) with 95% confidence intervals (CI) were pooled in a specific genetic model to assess the association and Stata version 14.0 software was used.A total of 9 studies with 1338 patients and 1677 controls were included in this study. The results from this meta-analysis indicated that TNF-α G-308A polymorphism significantly increased the risk of dilated cardiomyopathy in heterozygous comparison (GA versus GG: OR = 1.87; 95%CI = 1.03-3.40; P < 0.05). The increased risk of DCM was also found in Asian populations using a dominant model and heterozygous comparison (GA+AA versus GG: OR = 2.00, 95%CI = 1.02-3.92, P < 0.05; GA versus GG: OR = 1.94, 95%CI = 1.23-3.06, P < 0.05).The current meta-analysis revealed that TNF-α gene polymorphism (G-308A) may be associated with the susceptibility to DCM.
Subject(s)
Cardiomyopathy, Dilated/genetics , Genetic Association Studies/methods , Polymorphism, Single Nucleotide , Tumor Necrosis Factor-alpha/genetics , Cardiomyopathy, Dilated/ethnology , Female , Genetic Predisposition to Disease , Humans , Male , Observational Studies as TopicABSTRACT
BACKGROUND: Rapid progress has been made in research of cadmium-zinc-telluride (CZT) technology in the last few years, which might serve as a new method to diagnose coronary artery disease. However, compared with coronary angiography, the diagnostic value of CZT is still controversial. We aimed to evaluate diagnosis value of coronary angiography versus CZT in coronary artery disease. METHODS: We searched the database for eligible researches associated with CZT- myocardial perfusion imaging (MPI) and invasive coronary angiography, extracted the relevant data, and rigorously screened it according to the inclusion and exclusion criteria. The accuracy indicators included sensitivity, specificity, accuracy, positive and negative likelihood ratios. RESULTS: According to the inclusion and exclusion criteria, we finally found 20 studies containing 2350 patients in this search. Pooled results showed that sensitivity of CZT-MPI was 0.84% and 95% confidence interval (95% CI): 0.78 to 0.89, specificity was 0.72, 95% CI (0.62-0.76), the specificity was lower apparently. The positive likelihood ratio was 3.0, 95% CI (2.4-3.8), the negative likelihood ratio was 0.22, 95% CI (0.16-0.31), diagnostic odds ratio was 14, 95% CI (7.84-17.42). CONCLUSION: This meta-analysis showed that CZT-MPI had satisfactory sensitivity and specificity for diagnosing coronary artery disease. Larger studies are required for further evaluation.
Subject(s)
Coronary Angiography/methods , Coronary Artery Disease/diagnosis , Myocardial Perfusion Imaging/methods , Coronary Angiography/standards , Coronary Artery Disease/diagnostic imaging , Humans , Myocardial Perfusion Imaging/standards , Sensitivity and SpecificityABSTRACT
In the present study, a novel p-hydroxycinnamic amide (E)-3-(4-hydroxyphenyl)-N-(4-(N-(5-meth oxypyrimidin-2-yl)-sulfamoyl)phenyl)acrylamide (HMSP) was synthesized and confirmed. In vitro cytotoxic assays indicated that HMSP was able to inhibit the proliferation of various cancer cell lines. The interaction between HMSP and human serum albumin (HSA) was examined by fluorescence, UV-Vis and circular dichroism (CD) spectra, in addition to molecular simulation. The fluorescence and UV-Vis spectra data indicated that the binding of HMSP with HSA was a static process. According to the fluorescence quenching calculation, the corresponding thermodynamic parameters, bimolecular quenching rate constant and apparent quenching constants were calculated. Van der Walls forces and hydrogen bonds were vital in the binding of HMSP on HSA. The distances between HSA and its derivatives were obtained. Furthermore, competitive experiments and molecular modeling results suggested that the binding of the compound on HSA mainly occurred in site I (sub-domain IIA). Changes in HSA conformation were observed from synchronous fluorescence and CD spectra, which were further investigated by molecular dynamic simulations.
ABSTRACT
BACKGROUND: More susceptibility genes have been proved to be associated with coronary heart disease (CHD). The goal of our study is to evaluate the association between the R262W polymorphism of SH2B3 gene and risk of CHD. METHODS: A systematic search was conducted using PubMed, Embase, Web of Science, CNKI, and WanFang databases up to March of 2018. The data of individual study were individually performed by 2 reviewers. The meta-analysis was performed by Stata software and expressed by the pooled odds ratio (OR) and the 95% confidence interval (CI), which were calculated by specific model according to heterogeneity. RESULTS: Our research was based on 12 studies involving 25,845 patients and 68,910 healthy controls. Significant association between the variant R262W and CHD were found in overall populations (ORâ=â1.12, 95%CIâ=â1.09-1.15, Pâ=â.389, Iâ=â5.4%), but not found in Asian (ORâ=â1.05, 95%CIâ=â0.98-1.12, Iâ=â0.0%) in subgroup analysis by ethnicity. In another subgroup analysis, when classified into CHD and myocardial infarction (MI), there was a significance association between R262W and CHD (ORâ=â1.11,95% CIâ=â1.07-1.15, Iâ=â13.5%) and MI (ORâ=â1.13, 95%CIâ=â1.08-1.18, Iâ=â0.0%). The Begg's funnel plot revealed no significant publication bias. CONCLUSIONS: The R262W polymorphism is associated with risk of CHD or MI in Europeans, but not in Asians.
Subject(s)
Coronary Disease/genetics , Polymorphism, Single Nucleotide , Proteins/genetics , Adaptor Proteins, Signal Transducing , Case-Control Studies , Coronary Disease/metabolism , Genetic Predisposition to Disease , Humans , Intracellular Signaling Peptides and ProteinsABSTRACT
The authors are retracting this article [1]. In their recent work the authors have found that the degree of fibrosis in the different walls of the left atrium in pigs with hypertensive cardiomyopathy is the same. Sirius Red staining (another method to test for fibrosis) showed that there was no difference between the different walls. The authors are unable to explain this inconsistency. All authors agree with this retraction.
ABSTRACT
Generation of human cardiomyocytes from cells derived from various sources, including skin biopsy, has been made possible by breakthrough advances in stem cell research. However, it is attractive to build up a negligibly invasive way to create induced pluripotent stem (iPS) cells. In this study, we created iPS cells from human urine-derived epithelial cells by gene transduction using lentiviral vectors in a totally noninvasive manner. Then, we induced the differentiation of iPS cells into functional cardiomyocytes both in vitro and in vivo Action potentials were recorded in putative cardiomyocytes and spontaneous beating cells were observed. Our results offered an alternative method to generate cardiomyocytes in a totally noninvasive manner from an easily accessible source. The availability of urine and its potent reprogramming characteristics will provide opportunities for the use of cells with specific genotypes to study the pathogenesis and molecular mechanisms of disease in vitro.
ABSTRACT
Recently a large number of investigations have implicated the association between the chemokine CXC ligand 12 gene polymorphism (rs1746048) and risk of coronary heart disease (CHD), but the results remain debatable. The aim of our study was to provide more compelling evidence for the relationship between rs1746048 and CHD risk. Studies eligible for this meta-analysis were identified through electronic search of PubMed, EMBASE, and CNKI. Two authors performed independent literature review and study quality assessment by using the Newcastle-Ottawa Scale checklist. The odds ratios (ORs) with 95% confidence intervals (CIs) were pooled in a specific genetic model to assess the association. The meta-analysis of 48,852 patients and 64,386 controls from 12 studies showed that patients with rs1746048 had 1.11 times of high risk in developing CHD (ORâ=â1.11; 95% CIâ=â1.09-1.14; Pâ<â.005; Iâ=â35.8%). The increased risk of CHD was also found in both Asian (ORâ=â1.07; 95%CIâ=â1.02-1.12; Pâ<â.005; Iâ=â40.6%) and Caucasian populations (ORâ=â1.14; 95% CIâ=â1.10-1.18; Pâ<â.005; Iâ=â22.2%). The results of our meta-analysis suggested that chemokine CXC ligand 12 gene polymorphism (rs1746048) may be linked with susceptibility to CHD.
Subject(s)
Chemokine CXCL12/genetics , Coronary Disease/genetics , Genetic Predisposition to Disease , Polymorphism, Single Nucleotide , Coronary Disease/ethnology , Humans , Observational Studies as TopicABSTRACT
BACKGROUND: Previous case-control studies on association between KCNE1 G38S polymorphism and risk of atrial fibrillation (AF) have been published but because of the conflicting results and small sample size of individual studies, the consolidated result is still controversial. OBJECTIVES: The aim of this study was to explore the relationship between KCNE1 G38S polymorphism and risk of AF. METHODS: We performed a comprehensive literature search on PubMed, Embase, OVID, Web of Science, Wan Fang, and CNKI databases up to March 10, 2017 in English and Chinese languages. Two of the authors individually extracted study data and assessed the study quality using Newcastle-Ottawa scale. Odds ratios (ORs) and 95% confidence intervals (CIs) were combined in different genetic models for evaluation using a random-effect model or fixed-effect model according to interstudy heterogeneity. RESULTS: There were totally 14 independent case-control studies of 2810 patients and 3080 healthy controls included. Significant associations were found between KCNE1 G38S polymorphism and AF in overall population under all genetic models: allelic (OR: 1.34, 95% CI: 1.24-1.45, Pâ<â.001), homozygous (OR: 1.90, 95% CI: 1.61-2.24, Pâ<â.001), heterozygous (OR: 1.43, 95% CI: 1.21-1.68, Pâ<â.001), recessive (OR: 1.42, 95% CI: 1.20-1.69, Pâ<â.001), dominant genetic model (OR: 1.62, 95% CI: 1.39-1.89, Pâ<â.001). Subgroup analyses indicated similar association in Chinese and white. CONCLUSIONS: The G38S polymorphism in the KCNE1 gene can significantly increase the risk of AF in both Chinese and white.
Subject(s)
Atrial Fibrillation/genetics , Genetic Predisposition to Disease , Polymorphism, Genetic , Potassium Channels, Voltage-Gated/genetics , Atrial Fibrillation/ethnology , HumansABSTRACT
Heart failure with preserved ejection fraction (HFpEF), which is a primary driver of morbidity and mortality, accounts for approximately half of all heart failure cases. Therefore, it is essential to develop preclinical animal models for HFpEF pharmacological treatment strategies. We created a porcine model of severe hypertension and hyperlipidemia by using a combination of deoxycorticosterone acetate (DOCA, 100 mg kg-1), Western diet (WD) and angiotensin II infusion. Systolic blood pressure, echocardiography and invasive pressure-volume loop were assessed at baseline, 12 weeks and 18 weeks. A detailed histological assessment was also performed to determine the cardiac structural remodeling. Compared with controls (n=10), hypertensive animals (n=10) showed markedly higher systolic blood pressure (181 vs. 86 mm Hg) at 18 weeks. Concentric remodeling, characterized by a normal chamber size with a thicker wall, was observed in hypertensive animals. Left ventricle diastolic function showed a tendency toward decline, according to the echocardiographic data. Hemodynamic data showed that the end-diastolic pressure-volume relationship was elevated without changes in the end-systolic pressure-volume relationship. Histological results revealed that the fibrotic area in hypertensive animals (P<0.05 vs. controls) and the fibrotic area in the posterior wall of hypertensive animals' left atria were larger than other sites of the left atria (P<0.05 vs. other sites). This model can mimic clinical HFpEF to some degree. We found that the posterior wall of the left atrium is more susceptible to atrial remodeling associated with hypertension compared with other regions of the left atrium.
Subject(s)
Cardiomyopathies/pathology , Hypertension/pathology , Myocardium/pathology , Animals , Cardiomyopathies/diagnostic imaging , Desoxycorticosterone Acetate , Diet, High-Fat , Echocardiography , Female , Fibrosis/pathology , Hemodynamics , Hyperlipidemias/chemically induced , Hyperlipidemias/complications , Hyperlipidemias/pathology , Hypertension/diagnostic imaging , SwineABSTRACT
In the present study, nonylphenol (NP) was selected as the target pollutant to investigate the effect of biochar produced from wheat residue at different temperatures on loess soil based on the batch experiments. The research basically included adsorption kinetic, thermodynamic and some influencing factors such as biochar with different pyrolysis temperature, particle size and pH value. The results showed that the adsorption reaction of NP onto loess soil without biochar was 10 h during fast reaction, and after the addition of biochar into loess soil, the fast reaction time of NP adsorption was shortened. Meanwhile, in the fast stage the adsorption reaction of NP onto loess soil with biochar was significantly higher than loess soil without biochar, while the difference of adsorption capacity was small at different carbonization temperatures. The adsorption reaction of NP onto loess soil by adding biochar could be well described by the pseudo-second-order kinetics model and reached equilibrium in 16 h. The kinetic data showed that the adsorption of NP accorded well with the Freundlich isotherm model. The saturated adsorption capacity was improved as temperature increased with or without biochar. Thermodynamic parameter analysis indicated Gibbs free energy ΔGθ<0, entropy ΔHθ>0 and enthalpy ΔSθ>0, demonstrating it was a spontaneous, endothermic and chaos-increasing adsorption process. At the same temperature, the adsorption capacity of NP in loess soils increased dramatically with the increase of carbonization temperature. The smaller particle size of the loess with the addition of biochar, the better the adsorption of NP. When the pH value was 4 to 7, the adsorption capacity of NP onto loess soil by adding biochar showed an increasing trend; in the pH range of 7 to 10, the adsorption saturation capacity decreased with the increase of pH value. Therefore, the adsorption of NP on loess with the addition of biochar had the best adsorption effect in the neutral range. Acid and alkalinity were not conducive to the adsorption of NP.