Analysis of screening strategy of group B streptococcus in the third trimester and its influence on pregnancy outcome / 中华妇产科杂志

Objective To investigate the screening strategy of group B streptococcus (GBS) in the reproductive tract of women in the third trimester and analyze its impact on pregnancy outcome. Methods A total of 85 461 pregnant women in 35-37 weeks of gestation from Bao′an Maternity and Child Health Hospital, Jinan University from January 2011 to June 2018 were enrolled. They were divided into 3 periods according to different GBS screening strategies, the unscreened period included 31 384 cases (36.72%), 33 267 cases (38.93%) were included in partial screening period, 20 810 cases (24.35%) were included in screening period. All GBS screening positive pregnant women were given intrapartum antibiotic prophylaxis (IAP). The impact on pregnancy outcomes, and the impact of different GBS collection transport and culture methods on the positive rate of GBS screening were analyzed. Results (1) The incidence of neonatal early onset GBS disease (EOGBSD) in unscreened period was 0.03% (11/31 773), in partial screening period was 0.02%(6/33 887), and in screening period, the incidence of neonatal EOGBSD decreased to 0, the difference was statistically significant (χ2=7.86, P=0.02).(2) The incidence of hematogenous infection of GBS in pregnant women was 0.02%(6/33 887) in partial screening period, and there was none in screening period, there was no significant difference (adjusted χ2=3.75, P=0.05). (3) In the screening period, the positive rate of GBS was 14.08%(2 719/19 306), which was significantly higher than the positive rate of GBS in the partial screening period (11.48%, 2 058/17 920; χ2=56.12, P=0.00). (4) Antibiotic sensitivity tests of 4 777 GBS strains showed that the antibiotics with higher resistance rate were tetracycline (81.52%, 3 896/4 777), erythromycin (66.59%, 3 181/4 777), and clindamycin (64.31%, 3 072/4 777). The combination of erythromycin, clindamycin and tetracycline was the most common resistant pattern, accounting for 48.80% (2 331/4 777). No penicillin, ceftriaxone or vancomycin resistant strains was found. Conclusions GBS screening strategy in different regions could combine the local neonatal EOGBSD incidence rate, maternal GBS colonization rate, and the socioeconomic factors to determine whether universal GBS screening or screening for high-risk maternal women. GBS screening positive rate is related to the population, scope of the investigation, the sample collection, delivery and culture methods. The multi-drug resistance rate of GBS is high.[Key words] Streptococcus agalactiae; Streptococcal infections; Neonatal sepsis; Prenatal diagnosis; Pregnancy trimester, third; Pregnancy outcome

Establishment and evaluation of different serotypes group B streptococcus method by TaqMan fluorescent probe technique / 国际检验医学杂志

Objective To establish a molecular method for the identification of different serotypes of group B streptococcus(GBS)based on TaqMan fluorescence probe technology,and to lay the foundation for the sub-sequent study of multiple fluorescent probe technology to detect different serotypes of GBS.Methods Primers and probes were designed according to the different serotypes of capsular polysaccharide(CPS).CPS se-quences were amplified by real-time fluorescence quantitative polymerase chain reaction.GBS classification methods of different serotypes were established.The results were compared with latex agglutination test and the method was evaluated from the aspects of sensitivity,specificity and detection of clinical isolates.Results The logarithmic concentration of DNA in the same serotype GBS was linearly correlated with the value of Ct. The detection limit of this method is 1 pg/μL,a probe could only detect the corresponding serotype GBS.The results of TaqMan fluorescence probe test of 10 strains were consistent with the results of latex agglutination test.Conclusion TaqMan fluorescence probe technique is a simple,rapid,highly sensitive and specific method for the detection of different GBS serotypes,and it is better than latex agglutination test for the classification of clinical isolates.

The study on fetal hemoglobin in improving anemia of β-thalassemia carriers / 国际检验医学杂志

Objective To investigate the effect of fetal hemoglobin( HbF) in improving anemia of women who were β‐thalasse‐mia carriers at childbearing age .Methods 289 cases of women at childbearing age diagnosed as β‐thalassemia carriers in Shenzhen City were enrolled in this study .The high performance liquid chromatography (HPLC) was uesed for quantitative analysis of HbF , and the red blood cell parameters were detected by using the LH750 Automatic blood cell analyzer .The differences of red blood cell parameters between the HbF normal group and the HbF increased group were compared ,and the relationship between the high HbF expression rates and gene mutations were also analyzed .Results Comapred with the HbF normal group ,the hemoglobin (HGB) levels ,mean corpuscular volume (MCV) and the mean corpuscular hemoglobin (MCH) increased significantly ,while the red blood cell (RBC) count reduced ,there were significant differences between the two groups (P0 .05) .There were no significant differnces of rates of HBF high expression between different types of β‐globin gene mutations and the overall rate of HBF high expression (P>0 .05) .Conclusion Compared with the HbF normal group , anemia may improve more significantly in cases of women diagnosed as β‐thalassemia carriers at childbearing age in the HbF in‐creased group ,and there may be no relationship between gene mutations and high expression of HbF .

Impact of upper airway expansion surgery on the blood pressure of hypertension patients with upper airway obstruction / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the impact of upper airway expansion surgery on the blood pressure of hypertension patients with upper airway obstruction.@*METHOD@#Clinical data of 45 cases of hypertension patients with nasal obstruction who were underwent upper airway expansion surgery. The morning sitting blood pressure of patients were recorded. All of the patients were underwent nasal expansion surgery and 11 cases with obstructive sleep apnea hypopnea syndrome were combined with H-uvulopalatopharyngoplasty.@*RESULT@#Blood pressure of all 45 cases were apparently reduced in 12 months after the operations [systolic blood pressure (157.38 +/- 15.15) mmHg to (132.18 +/- 8.43) mmHg, diastolic blood pressure (95.42 +/- 9.28) mmHg to (82.31 +/- 5.88)mmHg], in which 9 cases with type I hypertension were lowered to normal pressure. Results had statistical difference between pre-operation and post-operation (P < 0.01).@*CONCLUSION@#Upper airway expansion surgery (including nasal and pharynx cav ity) can help lower blood pressure of hypertensive patients with nasal obstruction. Post-operative results were better than H-uvulopalatopharyngoplasty only.

Meroterpenoids and isoberkedienolactone from endophytic fungus Penicillium sp. associated with Dysosma versipellis / 药学学报

Seven meroterpenoids and five small-molecular precursors were isolated from Penicillium sp., an endophytic fungus from Dysosma versipellis. The structures of new compounds, 11beta-acetoxyisoaustinone (1) and isoberkedienolactone (2) were elucidated based on analysis of the spectral data, and the absolute configuration of 2 was established by TDDFT ECD calculation with satisfactory match to its experimental ECD data. Meroterpenoids originated tetraketide and pentaketide precursors, resepectively, were found to be simultaneously produced in specific fungus of Penicillium species. These compounds showed weak cytotoxicity in vitro against HCT-116, HepG2, BGC-823, NCI-H1650, and A2780 cell lines with IC 50 > 10 micromol x L(-1).

Auditory Condition and Hearing Aids in 91 Adults with Hearing-impairment / 中国康复理论与实践

Objective To investigate the auditory condition in adults with hearing-impairment and evaluate the outcome of the hearing aid. Methods The threshold of air-bone-conduction audiometry and binaural hearing aid in 91 adults were determined with pure tone audi-ometry, and their satisfaction to hearing aids were investigated with free interview. Results The threshold of hearing and hearing aid were positively correlated. The satisfaction did not significantly correlate with the threshold of hearing aid, but with the time of deprivation of hearing. The hearing threshold classification was better in the patients with the audiogram of flat, raising, slow down type than with audio-gram of Shu type, which defined as hearing reacted only in 1 or 2 frequency. Conclusion It is important to improve the primary otology pro-tection, as well as the rehabilitation from the hearing aids.

Studies on chemical constitutes from callus cultures of Stellera chamaejasme / 中国中药杂志

From callus cultures of Stellera chamaejasme, 17 compounds were isolated. Based on their physical and chemical data and spectroscopic analysis, they were identified as syringaresinol (1), medioresinol (2), pinoresinol (3), (1R, 2S, 5R, 6S)- 2-(4- hydroxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3, 7-dioxabicyclo [3, 3, 0] octane (4), epipinoresinol (5), caruilignan D (6), 3-oxo-guai-4-ene-11, 12-diol (7), (-) -lariciresinol (8), tetrahydro-2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxyphenyl) methyl]-3-furanmethanol (9), 5'-methoxylariciresinol (10), vladinol D (11), cyclo (L-Pro-L-Val) (12), oxomatairesinol (13), (+) -guayarol (14); acutissimalignan B (15), isolariciresinol (16), and beta-sitosterol (17), respectively. Among these compounds, 12 was a cyclodipeptide, 7 was a sesquiterpene, and the others except 17 were lignans. All compounds were first isolated from callus cultures of S. chamaejasme.

Studies on metabolites from marine microorganism Aspergillus terreus collected from nature reserve region of mangrove / 中国中药杂志

<p><b>OBJECTIVE</b>To search for new antitumor active lead compounds from marine microorganism.</p><p><b>METHOD</b>A marine strain, Aspergillus terreus, was cultured and up-scaled in artificial seawater media, from which the metabolites were isolated and elucidated by using modern spectroscopy techniques.</p><p><b>RESULT</b>Twelve compounds were isolated from mycelia and fermentation broth of A. terreus.</p><p><b>CONCLUSION</b>Compounds 1-4 were steroids, compounds 5-8 were organic acids and esters, compound 9 was an alkaloid, compound 10 was an isocoumarin, compound 11 was ceramide, compound 12 was propenyl cyclic pentanediol.</p>

Lactone constituents of Saussurea involucrata / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Saussurea involucrata.</p><p><b>METHOD</b>Compounds were isolated and purified by column chromatography, and their structures were elucidated by chemical and spectroscopic methods.</p><p><b>RESULT</b>Eight compounds were yielded and identified as beta-sitosterol (1), dotriacontanoic acid (2), hexacosan-4-olide (3), (+)-11alpha, 13-dihydrodehydrocostuslactone (4), 8alpha-acetoxydehydrocostus lactone (5), zaluzanin C (6), 11,13beta-dihydrozaluzanin C (7), 8alpha-hydroxy-11beta-11,13-dihydrodehydrocostuslactone (8).</p><p><b>CONCLUSION</b>Compounds 2-7 were first isolated from S. involucrate.</p>

Delayed scanning method in multi-slice spiral CT for diagnosis of pulmonary embolism / 中国介入影像与治疗学

Objective To compare different delayed scanning methods in multi-slice spiral CT angiography (MSCTA)in diagnosis of pulmonary embolism (PE). Methods Sixty patients with suspected PE were divided into three groups (A, B and C). MSCT with same Iodine concentration, injection rate, contrast medium but different delayed scanning methods was performed after injection of contrast medium. Patients in group A were examined with fixed time method (15 s), in group B with small dose-density curve method, while in group C with contrast medium track and triggering technoligy. The number, position and the shapes of emboli were evaluated with MIP, MPR and VR. Results The successful examination rate of group A was 55.00% (11/20), while of group B and C was both 100%. The coincidence rate of MSCTA compared with DSA in each group was 96.04% (291/303). Conclusion The best delayed scanning method in MSCTA for diagnosis of pulmonary embolism is contrast medium tracking and triggering technology.

Image examination of renal injuries and analysis of renal explorative indications / 中华创伤杂志

Objective To study the image examination of renal injuries and discuss renal explorative indications so as to spare the kidney or nephron as much as possible and improve curative rate of diagnosis and treatment. Methods An analysis was done on 286 cases that included 231 cases with close injury, 54 with open injuries, one with iatrogenic injury and 91 with combined injuries. Of all, 212 cases were examined by B-ultrasonography, 163 by CT and 132 by intravenous urography(IVU) and 6 by digital subtraction angiography(DSA); 202 cases were treated with conservative treatment and 84 with operation. Results The diagnostic positive rates of IVU, B-ultrasonography and CT were 67.4%, 72.2% and 87.7%, respectively. Among the operation cases, 42 cases were treated by renal repair, 12 by partial nephrectomy and 30 by nephrectomy. The operation rate was 29.4% and the nephrectomy rate 35.5%. Interventional treatment of the kidney was carried out in three cases. Conclusions For renal injury cases, the first and most important step is to evaluate the injury condition so as to correctly determine whether an operation exploration is needed. The injury conditions and severity are mainly determined by the image examinations that change according to injury cause, injury type and clinical symptoms. Renal exploration or not, and the operation time exert great influence on renal reservation rate and complication rate.

Follow up Study of Membranous Budd-Chiari Syndrome Treated By Percutaneous Transluminal Angioplasty / 实用放射学杂志

8 years for 4 cases;5~8 years for 8 cases;3~5 years for 3 cases;less than 1 year for 1 case respectively.12 cases were followed up by color-sonography examination.The remaining 6 cases were followed by mail or phonecall.Results All cases reached clinical cure with relief or diasppearance of relevant symptoms and signs after 2~7 days PTA.88.9%(16/18) of the cases regained obility to labour or return to work.2 cases of the patients had lung infarction and heart failure immediately after the treatment,1 patient had recurrent stenosis and thrombosis after 1 year of PTA.Conclusion For treatment of membranous BCS,PTA should be the first choice.The majority methods of preventing recurrent stenosis and thrombosis are antithrombin application before PTA and adequate angioplasty during PTA.