Comparative study of different cocoa (Theobroma cacao L.) clones in terms of their phytoprostanes and phytofurans contents.

Cocoa has been widely discussed as a bioactive food rich in sensory stimulation and health benefits. However, no information has been provided concerning phytoprostanes (PhytoPs) and phytofurans (PhytoFs) in cocoa. These compounds are of interest because they play a role in the regulation of immune function. The present study included 31 cocoa clones. The PhytoPs and PhytoFs were quantified by UHPLC-QqQ-MS/MS. The total PhytoPs and PhytoFs contents ranged from 221.46 to 1589.83 ng g-1 and from 1.18 to 13.13 ng g-1, respectively. The profiles of the PhytoPs and PhytoFs identified in the cocoa beans showed significant differences among the clones analysed. The results indicate that dry fermented cocoa beans are rich in PhytoPs and PhytoFs, which may represent an additional benefit of the consumption of foods derived from cocoa.

Potential of Physalis peruviana calyces as a low-cost valuable resource of phytoprostanes and phenolic compounds.

BACKGROUND: In Colombia, agro-industrial residues represent an enormous economic and environmental problem, which could be reduced if different techniques for the addition of value to such residues were implemented by this industrial sector. One of the fruits with the highest export rates is Physalis peruviana (goldenberry); however, this fruit is generally marketed without its calyx, generating a large amount of residues. To develop a strategy to add value to these residues, it is essential to know their chemical composition. RESULTS: In the present work, phytoprostanes (PhytoPs) - new active oxylipins - have been detected for the first time in Physalis peruviana calyces by ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS), F1t -phytoprostanes and D1t -phytoprostanes being the predominant and minor classes, respectively. In addition, we were able to characterize the phenolic compounds profile of this matrix using LC-IT-DAD-MS/MS, describing six phenolic derivatives for the first time therein. CONCLUSIONS: This study increases our knowledge of the chemical composition of the calyces of this fruit and thereby supports the recycling of this class of residue. Consequently, goldenberry calyces could be used as phytotherapeutic, nutraceutic, or cosmetic ingredients for the development of diverse natural products. © 2018 Society of Chemical Industry.

Microencapsulation of lutein by spray-drying: Characterization and stability analyses to promote its use as a functional ingredient.

Lutein, a xanthophyll, is associated to decreased risk of age-related macular degeneration, atherosclerosis and other diseases (Bovier et al., 2013; El-raey, Ibrahim, & Eldahshan, 2013). When lutein is extracted, it becomes highly unstable, reducing its functionality as an antioxidant. The aim of this research was to improve the stability of lutein using maltodextrin, arabic gum and a modified starch, to obtain micro-particles using spray-drying. Each of the formulations was characterized in terms of yield, encapsulation efficiency, particle size distribution, water activity and moisture content. The formulations with arabic gum (100%) and arabic gum:maltodextrin:modified starch (33.3:33.3:33.3%), with encapsulation efficiencies of 91.94 ±â€¯6.88 and 65.72 ±â€¯0.93%, respectively, were selected to study stability at 45 °C and 75% RH (relative humidity). Based on our results, encapsulation could be considered as an alternative for the generation of high value-added functional ingredients that can be used in different industries.

Untargeted metabolomics reveals specific withanolides and fatty acyl glycoside as tentative metabolites to differentiate organic and conventional Physalis peruviana fruits.

The agronomic production systems may affect the levels of food metabolites. Metabolomics approaches have been applied as useful tool for the characterization of fruit metabolome. In this study, metabolomics techniques were used to assess the differences in phytochemical composition between goldenberry samples produced by organic and conventional systems. To verify that the organic samples were free of pesticides, individual pesticides were analyzed. Principal component analysis showed a clear separation of goldenberry samples from two different farming systems. Via targeted metabolomics assays, whereby carotenoids and ascorbic acid were analyzed, not statistical differences between both crops were found. Conversely, untargeted metabolomics allowed us to identify two withanolides and one fatty acyl glycoside as tentative metabolites to differentiate goldenberry fruits, recording organic fruits higher amounts of these compounds than conventional samples. Hence, untargeted metabolomics technology could be suitable to research differences on phytochemicals under different agricultural management practices and to authenticate organic products.

Quantification of phytoprostanes - bioactive oxylipins - and phenolic compounds of Passiflora edulis Sims shell using UHPLC-QqQ-MS/MS and LC-IT-DAD-MS/MS.

The genus Passiflora, comprising about 500 species, is the largest in the Passion flower family. Passiflora edulis Sims f. edulis (gulupa) is one of the most important fruits cultivated in Colombia. In recent years and due to its organoleptic and bioactive properties, its exports have significantly increased. In this work, six new bioactive oxylipins -phytoprostanes - were detected in gulupa shell by a UHPLC-QqQ-MS/MS method: F1t-phytoprostanes and D1t-phytoprostanes were the predominant and minor classes, respectively. Moreover, the polyphenol profile of the shell was investigated and we were able to detect and quantify phenolic compounds that have not been described previously, like luteolin-8-C-(2-O-rhamnosyl)hexoside and quercetin-3-O-(6″-acetyl)glucosyl-2″-sinapic acid. Consequently, this study provides new insights into the importance of gulupa shell as a valuable option in the design of new beverages rich in antioxidant phytochemicals, as part of a well-balanced diet, and in the process and quality control of such products.

Percepción de estudiantes universitarios consumidores de psicotrópicos sobre políticas que abordan esta problemática en Medellín, Colombia / Consumer conceptions of college students of psychotropic consumption sgents as a public health problem in Medell¡n, Colombia

Objetivo: Indagar sobre la percepción de estudiantes universitarios consumidores de agentes psicotrópicos frente a las políticas universitarias para intervenir este problema de salud pública de origen multicausal. Método: Estudio de casos con participación de 45 estudiantes escogidos a conveniencia. Se aplicó una entrevista semiestructurada voluntaria y anónima dirigida por una encuesta y aplicación del test de Zung. Resultados: Se indicó una correlación entre el consumo y las variables psicosociales como las razones para consumir y las consecuencias de éste. El test de Zung no evidencia asociación alguna entre el consumo de psicotrópicos y la depresión. Se concluye corresponsabilidad de los estudiantes para responder de forma adecuada a un problema tan antiguo como nuevo dentro del ámbito universitario...

Development of an analytical procedure for the determination of emerging and priority organic pollutants in leafy vegetables by pressurized solvent extraction followed by GC-MS determination.

A new multiresidue method for the determination of 13 emerging and priority pollutants in lettuce, including pesticides, pharmaceuticals, personal care products, polycyclic aromatic hydrocarbons (PAHs), and phenolic estrogens, has been developed using matrix solid-phase dispersion combined to pressurized fluid extraction (PFE) followed by gas chromatography coupled to mass spectrometry determination. A sequential optimization strategy based on solvent optimization first, followed by experimental design, was performed in order to maximize target analyte extraction with the aid of response surface methodology. Firstly, a full factorial design was applied to choose the significant variables in PFE; extraction time and temperature were found to have the biggest overall effect on response for most of analytes. They were later optimized performing a central composite design and the variable response of these factors was modeled for all analytes. It was found that marked differences in physicochemical nature exerted a strong influence on extraction conditions and yield. Therefore, the effect of parameters on the response was rather different for some compounds. To overcome this conflicting behavior, a multiple response simultaneous optimization was applied using the desirability function to achieve global optimal operating conditions. The optimal conditions were attained at 13.5 min (two extraction cycles) and 104 degrees C in the PFE by using hexane acetone mixture (1:1). Limit of detection and limit of quantitation values were found to be between 6.6 and 58 and 7.6 and 61.7 microg kg(-1), respectively.