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Anaerobic ammonium oxidation (anammox), an energy-efficient deamination biotechnology, faces operational challenges in low-temperature environments. Enhancing the metabolic activity of anammox bacteria (AnAOB) is pivotal for advancing its application in mainstream municipal wastewater treatment. Inspired by the metabolic adaptability of AnAOB and based on our previous findings, this work investigated the enhancement of intracellular ATP and NADH synthesis through the exogenous supply of reduced humic acid (HAred) and H2O2 redox couple, aiming to augment AnAOB activity under low-temperature conditions. Our experimental setup involved continuous dosing of 0.0067 µmol g-1 volatile suspended solid of H2O2 and 10 mg g-1 volatile suspended solid of HAred into a mainstream anammox reactor operated at 15 °C with an influent TN content of 60 mg/L. The results showed that HAred / H2O2 couple succeeded in maintaining the effluent TN at 10.72 ± 0.91 mg l-1. The specific anammox activity, ATP and NADH synthesis levels of sludge increased by 1.34, 2.33 and 6.50 folds, respectively, over the control setup devoid of the redox couple. High-throughput sequencing analysis revealed that the relative abundance of Candidatus Kuenenia after adding HAred / H2O2 couple reached 3.65 % at the end of operation, which was 5.14 folds higher than that of the control group. Further metabolomics analysis underscored an activation in the metabolism of amino acids, nucleotides, and phospholipids, which collectively enhanced the availability of ATP and NADH for the respiratory processes. These findings may provide guidance on strategy development for improving the electron transfer efficiency of AnAOB and underscore the potential of using redox couples to promote the mainstream application of anammox technology.
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Oxidation-Reduction , Sewage , Sewage/microbiology , Bioreactors , Ammonium Compounds/metabolism , Anaerobiosis , Hydrogen Peroxide/metabolism , Waste Disposal, Fluid/methods , Bacteria/metabolism , NAD/metabolismABSTRACT
Objective@#To explore the effects of community building environment and sports with fitness APP usage and their interactions on teachers exercise habits in the Yangtze River Delta Region, so as to provide a scientific basis for the development of a sports and health promotion intervention program for teachers.@*Methods@#A total of 2 530 in service teachers from four provinces and cities in the Yangtze River Delta region, namely, Shanghai, Zhejiang, Jiangsu and Anhui Province, were sampled in May-June 2023 by using convenient cluster random sampling method. Self designed questionnaire was used to collect the basic information of the surveyed teachers, Physical Activity Building Environment Evaluation Questionnaire and the Sports with Fitness APP Usage Questionnaire were used to measure the teachers subjective perception of the community building environment and the usage of sports with fitness APP, respectively. Physical Exercise Habituation Scale was used to assess the level of exercise habits. Logistic regression models were applied to analyze the effects of community building environment and sports with fitness APP usage on physical exercise, and the interaction effects were analyzed by using additive and multiplicative models.@*Results@#Among all the teachers surveyed, 658 of them reported good physical exercise habits (26.0%), and differences in the rate of physical activity habit formation by gender, age, years of teaching, as well as subject of teaching were statistically significant ( χ 2=42.94, 39.73, 35.47, 218.23 , P <0.05). Teachers with physical exercise habits had significantly higher community building environment scores and sports and fitness APP use than teachers without exercise habits ( t =12.17,16.54, P <0.05). Adjusting for the confounders of age, gender, years of teaching experience, and subjects taught, multifactorial unconditional Logistic regression analysis showed that the probability of teachers having good physical exercise habits increased by 22% for every 1-point increase in the community building environment score on average ( OR =1.22, 95% CI =1.11-1.40), and the probability of teachers having good physical exercise habits increased by 16% for every 1-point increase in the sports with fitness APP score on average ( OR = 1.16 , 95% CI =1.03-1.31) ( P <0.05). Interaction analyses showed that there was an additive interaction between the effects of community building environment and sports and fitness APP use on teachers physical exercise habits after adjustment, and the 95% CI for RERI , API and SI were 1.17 -1.65, 0.12-0.46 and 1.78-3.33 ( P <0.05), respectively, and there was no multiplicative interaction ( P >0.05).@*Conclusions@#The community building environment and the usage of sports & fitness APP show impacts in the formation of teachers physical exercise habits in the Yangtze River Delta region, and there is an interaction effect. Enhancing the construction of smart sports centers around the community can provide a high quality external environment for the physical exercise habits formation.
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We experimentally study the tunability of second harmonic generation (SHG) from a two-dimensional (2D) material in a 2D material/dielectric film/substrate layered structure. Such tunability arises from two interferences: one is between the incident fundamental light and its reflected light, and the other is between the upward second harmonic (SH) light and the reflected downward SH light. When both interferences are constructive, the SHG is maximally enhanced; it becomes attenuated if either of them is destructive. The maximal signal can be obtained when both interferences are perfectly constructive, which can be realized by choosing a highly reflective substrate and an appropriate thickness for a dielectric film that has a large difference in its refractive indices at the fundamental and the SH wavelengths. Our experiments demonstrate variations of three orders of magnitude in the SHG signals from a monolayer MoS2/TiO2/Ag layered structure.
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ObjectiveTo develop and validate a predictive risk model for vision-threatening diabetic retinopathy in patients with type 2 diabetes using readily accessible clinical data, which may provide a convenient and effective prediction tool for early identification and referral of at-risk populations. MethodsA nomogram model was developed using a dataset obtained from patients with T2DM who participated in the Guangzhou Diabetic Eye Study from November 2017 to December 2020. Logistic regression was used to construct the model, and model performance was evaluated using receiver operating characteristic curve, Hosmer-Lemeshow test, calibration curve and decision curve analysis. The model underwent internal validation through the mean AUC of k-fold cross-validation method, and further external validation was conducted in the Dongguan Eye Study. ResultsA total of 2 161 individuals were included in the model development dataset, of whom 135 (6.25%) people were diagnosed with VTDR. Age (P<0.001,OR=0.927,95%CI:0.898~0.957) and body mass index (P<0.001,OR =0.845,95%CI:0.821~0.932) were found to be negatively correlated with VTDR, whereas diabetes duration (P<0.001,OR=1.064,95%CI:1.035~1.094), insulin use (P =0.045,OR =1.534,95%CI:1.010~2.332), systolic blood pressure (P<0.001,OR =1.019,95%CI:1.008~1.029), glycated hemoglobin (P<0.001,OR =1.484,95%CI:1.341~1.643), and serum creatinine (P<0.001,OR =1.017,95%CI:1.010~1.023) were positively correlated with VTDR. All these variables were included in the model as predictors. The model showed strong discrimination in the development dataset with an area under the receiver operating characteristic curve (AUC) of 0.797 and in the external validation dataset (AUC 0.762). The Hosmer-Lemeshow test(P>0.05)and the calibration curve displayed good agreement. Decision curve analysis showed that the nomogram produced net benefit in the two datasets. ConclusionsIndependent factors influencing VTDR include age, duration of diabetes mellitus, insulin use, body mass index, systolic blood pressure, glycosylated hemoglobin, and serum creatinine. The nomogram constructed using these variables demonstrates a high degree of predictive validity. The model can serve as a valuable tool for early detection and referral of VTDR in primary care clinics. Therefore, its application and promotion are highly recommended.
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Objective@#To understand the association between sugar sweetened beverage consumption with physical fitness index among college students, so as to provide a reference for the improvement and intervention of college students physical fitness.@*Methods@#From September to December 2021, a total of 5 520 college students from universities in Jilin, Anhui, Jiangxi, Xinjiang, and Shanghai, were surveyed with sugar sweetened beverage consumption and physical fitness using stratified cluster sampling. One way ANOVA and Logistic regression analysis were used to analyze the association between sugary drink consumption and physical fitness index (PFI).@*Results@#The frequencies of sugar-sweetened beverage consumption among college students were 10.3% , 67.2%, 13.9% , and 8.6% for 0, 1-3, 4-5, and ≥ 6 times/week, respectively. The differences in waist circumference, grip strength, standing long jumping, sit and reach, 50-meter running, and 1 000/800 meter running performance were statistically significant among college students with varying sugar-sweetened beverage consumption ( F =8.67, 7.22, 11.20, 13.47, 3.98, 2.86 , P <0.05). Logistic regression analysis showed that those who had sugary drinks 4-5 times/week, ≥ 6 times/week had a higher risk of low PFI compared to those who reported no sugary drinks ( OR =1.48, 1.56, P <0.05). With respect to gender, the risk of low PFI was also higher among male students who consumed sugary drinks ≥6 times/week ( OR =2.01, P <0.05), while there was no significant difference among female students.@*Conclusion@#Sugar-sweetened beverage consumption is negatively associated with the physical fitness index among college students with significant gender differences. College students, especially males, should be targeted to improve health literacy, reduce the consumption of sugar-sweetened beverages, so as to improve physical fitness.
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To assess the association between serum vitamin D levels and heart rate variability in patients with type 2 diabetes mellitus (T2DM). This study included 469 patients who were retrospective assessed for eligibility from Changzhou Second People's Hospital, Affiliated Nanjing Medical University, between March 2013 and June 2020. A total of 191 T2DM patients were recruited and divided into 3 groups. A total of 191 patients were recruited. A significant difference was noted among groups for HbA1c (P < .001), serum uric acid (P = .048), and urea nitrogen (P = .043). The Vitamin D level in deficiency, insufficiency, and sufficient was 23.17, 38.89, and 63.01 nmol/L, respectively. The insufficient group had lower levels of percentage of normal-to-normal intervals differing by more than 50 milliseconds, and the square root of the mean of the squares of the differences between adjacent normal-to-normal R peak-to-R-peak time intervals than the sufficient vitamin D group. Furthermore, patients in deficiency and insufficiency group were associated with high level of low frequency power/high frequency power as compared with sufficient vitamin D group. Finally, serum 25-hydroxyvitamin D (25(OH)D) levels were positively correlated with rMSSD (P = .002). This study found that low serum 25(OH)D levels were associated with reduced heart rate variability parameters in patients with T2DM.
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Diabetes Mellitus, Type 2 , Vitamin D Deficiency , Diabetes Mellitus, Type 2/complications , Heart Rate/physiology , Humans , Retrospective Studies , Uric Acid , Vitamin D , Vitamin D Deficiency/complications , VitaminsABSTRACT
OBJECTIVE@#To explore the association between postoperative C-reactive protein (CRP) levels and the occurrence of postoperative atrial fibrillation in patients undergoing cardiopulmonary bypass surgery.@*METHODS@#We retrospectively analyzed the data of 550 patients undergoing cardiopulmonary bypass surgery in our hospital from September, 2018 to May, 2021, and after screening against the exclusion criteria, 363 patients were selected for further analysis. Univariate analysis was used to analyze the correlation of age and early postoperative CRP level with the occurrence of postoperative atrial fibrillation, and Chi-square test was used to explore the correlation of gender, disease type, and comorbidity with postoperative atrial fibrillation followed by multivariate analysis of the data using a binary logistic regression model.@*RESULTS@#The 363 patients enrolled in this study included 247 with valvular disease, 42 with aortic dissection, 37 with coronary heart disease, and 37 with congenital heart disease, with a median postoperative CRP level of 88.65 mg/L and a median age of 57 years (range 5-77 years). Postoperative atrial fibrillation occurred in 101 (27.82%) of the patients, who were subsequently divided into atrial fibrillation group and sinus group. Univariate and multivariate correlation analyses showed that early postoperative elevation of CRP level was an important factor contributing to the occurrence of postoperative atrial fibrillation.@*CONCLUSION@#Early postoperative elevation of CRP level is associated with the occurrence of atrial fibrillation following cardiopulmonary bypass surgery.
Subject(s)
Adolescent , Adult , Aged , Child , Child, Preschool , Humans , Middle Aged , Young Adult , Atrial Fibrillation/etiology , C-Reactive Protein/analysis , Cardiopulmonary Bypass/adverse effects , Coronary Artery Bypass , Postoperative Complications/epidemiology , Retrospective Studies , Risk FactorsABSTRACT
Due to the particularity of assisted reproductive technology, almost all colleges and universities have not included the reproductive technology experiment course into the scope of postgraduate teaching. Based on the rapid development of the reproductive medicine and the shortage of qualified personnel, through the questionnaire survey, the paper investigates the willingness of reproductive medicine postgraduate students to learn assisted reproductive technology, the current status of experimental courses on assisted reproductive technology, student satisfactory with the courses, the necessity of setting up an experimental course for assisted reproductive technology, and so on. By analyzing the current situation of postgraduate teaching in reproductive medicine and the results of questionnaire survey, we explores the necessity and limitations of strengthening laboratory teaching of assisted reproductive technology in the process of reproductive medicine postgraduate training, and proposes corresponding teaching strategies.
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INTRODUCTION: Different types of ketosis-prone obese diabetic patients are seen in the clinic. At present, the mechanism responsible for ketosis onset in these patients remains unclear, and we do not know how these patients should be optimally treated to prevent recurrent ketosis. Therefore, this study aims to investigate risk factors of ketosis in obese ketosis-prone diabetic (OB-KPD) patients. METHODS: In an observational case-control study, primary OB-KPD patients [body mass index (BMI) ≥ 28 kg/m2] were selected as the study group (OB-KPD group), and primary obese type 2 diabetes patients served as the control group (OB-T2DM group). Clinical diagnostic assessments of fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), blood lipid, area under curve of serum C-peptide (AUCC-P) after steamed bread meal, insulin sensitivity index (ISI), ß-hydroxybutyric acid (ß-HB) and free fatty acid (FFA) vlaues of the subjects were collected. Subjects in the OB-KPD group were followed up for 1 year to determine the likelihood of insulin therapy cessation and whether ketosis recurred by assessing clinical chemistry parameters at 1-year follow-up. RESULTS: Seventy-five subjects were screened, of which 15 were not included in the study for several identified clinical reasons. On enrollment, the OB-KPD group displayed significantly higher FPG, HbA1c and FFA levels than the OB-T2DM group (p < 0.01), while AUCC-P and ISI values were significantly lower than in the OB-T2DM group (p < 0.01 and p = 0.03). Statistical analysis showed that increases in ß-HB in the OB-KPD group were associated with increased blood glucose and FFA and decreased AUCC-P and ISI values. Furthermore, decreases in AUCC-P were closely associated with increased blood glucose values. CONCLUSION: The occurrence of ketosis in ketosis-prone obese diabetic patients may be related to glucose and lipid metabolism disorders, increased insulin resistance and decreased ß-cell secretory functions. TRIAL REGISTRATION: This work was registered at the Chinese Clinical Trial Registry with trial registration identifier no. ChiCTR1900025909.
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BACKGROUND: To delay the further aggravation of early femoral head necrosis, drilling decompression of the femoral head is a better treatment method. However, traditional trepanation and decompression of the femoral head is traumatic. OBJECTIVE: To compare the difference in the effect between robot-assisted drilling decompression and traditional surgery for aseptic necrosis of femoral head. METHODS: Forty patients with aseptic necrosis of the femoral head were included for retrospective comparative analysis. According to the treatment plan, they were divided into two groups. Eighteen cases (26 femoral heads) in the robot group were treated with drilling decompression assisted by robots. Among them, 13 cases (18 femoral heads) were in Ficat stage I and 5 cases (8 femoral heads) were in Ficat stage II. The traditional surgery group consisted of 22 patients (29 femoral heads). Among them, 15 patients (19 femoral heads) were in Ficat stage I and 7 patients (10 femoral heads) in Ficat stage II. Incision, times of intraoperative fluoroscopy, intraoperative blood loss, times of intraoperative puncture and operation time were compared between the two groups. Harris score was used before and 1, 3, and 6 months after operation to evaluate the hip effect. RESULTS AND CONCLUSION: (1) All cases were followed up for 6 months. (2) Incision, times of intraoperative fluoroscopy, intraoperative blood loss, times of intraoperative puncture and operation time were better in the robot group than in the traditional surgery group (P 0.05). (4) Compared with the traditional surgery, robot-assisted drilling decompression has no obvious advantage in the treatment of aseptic necrosis of the femoral head. However, its operation incision is small; the number of intraoperative fluoroscopy is small; the trauma is small; and the operation is safer and minimally invasive.
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Objective:To investigate the levels of gross radioactivity in drinking water and of 90Sr in food around Sanmen nuclear power plant (NPP) before and after operation. Methods:From 2012 to 2019, 7 drinking water monitoring sites were selected around the Sanmen NPP to collect source water, factory water and peripheral water, respectively, in order to measure and analyze the total radioactivity level in drinking water. Based on the dietary habits of local residents, 4 kinds of food such as grains, vegetables, freshwater fish and marine fish grown locally were sampled to measure and analyze the activity concentration of 90Sr. Results:Before the operation of Sanmen NPP, the total α radioactivity and total β radioactivity in drinking water around the NPP were 0.001-0.063 Bq/L and 0.019-0.210 Bq/L, respectively. After operation, the total α radioactivity and total β radioactivity were 0.001-0.084 Bq/L and 0.025-0.079 Bq/L, respectively. Both were lower than the national standard value given in the GB 5749-2006 " Sanitary standards for drinking water" . There was no significant change in the radioactivity level of source water, factory water and peripheral water during the wet and dry periods. The activity concentration of 90Sr in these four types of food ranged from 0.037 to 1.216 Bq/kg before operation and from 0.049 to 0.692 Bq/kg after operation, all lower than the standard value of " Standard for the concentration in radioactive substances in food" (GB 14882-94). Conclusions:Before and after the operation of Sanmen NPP, the total α and total β radioactivity in drinking water and 90Sr activity concentration in food are stable, below the national standard limit.
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To evaluate the bone strength and structure of patients with obstructive sleep apnea(OSA)by the high-resolution peripheral quantitative computed tomography(HR-pQCT)and to explore the relationship between OSA and osteoporosis. Male patients who visited the Sleep Respiratory Center of our hospital from August 2017 to January 2019 were consecutively recruited.Clinical data including the results of Epworth sleep scale(ESS)scoring and overnight polysomnography were collected.HR-pQCT was used to compare the differences in bone geometry,density,and microstructure between OSA patients and non-OSA people;also,the radius and tibia on the non-dominant side were measured to explore the relationship between OSA and osteoporosis. A total of 83 subjects were enrolled in the study.The number of patients in the mild,moderate,and severe OSA groups and non-OSA group were 21,18,34,and 10,respectively.There was no significant difference in age,blood pressure,ESS score,sleep stage,and sleep efficiency among these four groups(>0.05).Body mass index(BMI)and neck circumference were significantly different among these groups(=4.234,=0.008;=3.100,=0.031).There was no significant difference in the radius indicators(>0.05).For tibia,there were significant differences among the four groups in the cortical area(Ct.Ar)(=3.937,=0.011).There were also significant differences in the bone microstructural indicators including trabecular thickness(Tb.Th)and cortical thickness(Ct.Th)(=6.247,=0.001;=3.746,=0.014),which were significantly lower in the three OSA groups than in the control group.Pairwise comparisons showed that the Ct.Ar in the severe OSA group was significantly higher than that in the mild OSA group(=0.019)and Tb.Th in the control group was significantly higher than those in the mild and moderate OSA groups(=0.006,=0.001).Correlation analysis showed that,within a certain range,total volumetric bone mineral density(Tt.vBMD)and Tb.Th of radius and tibia were negatively correlated with age(=-0.312,=0.004;=-0.328,=0.002;=-0.265,=0.015;=-0.280,=0.010)and positively correlated with BMI(=0.240,=0.029;=0.369,=0.004;=0.299,=0.006;=0.416,=0.010).Stepwise multiple regression analysis showed that Tb.Th of radius and tibia were mostly correlated with BMI(=0.262,=0.008,=0.243,=6.270,=0.000;=0.494,=0.000,=0.186,=7.243,=0.000)and age(=-0.216,=0.030,=0.243,=6.270,=0.000;=-0.306,=0.003,=0.186,=7.243,=0.000).Tt.vBMD of radius had a certain correlation with sleep efficiency and with the decreasing nocturnal mean oxygen saturation caused by OSA(=0.312,=0.002, =-0.249,=0.012,=0.327,=7.482,=0.000). In non-elderly male populations,OSA mainly causes a decrease in Tb.Th and Ct.Th of the tibia.The changes in bone strength and structure are mainly related with age and body size and also have certain correlations with sleep efficiency and with the decreasing nocturnal mean oxygen saturation caused by OSA.
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Humans , Male , Bone Density , Bone and Bones , Polysomnography , Sleep Apnea, Obstructive , Tomography, X-Ray ComputedABSTRACT
Objective To study the vascular anatomy of duplex kidney and to provide a scientific basis for surgical resection of the renal segment.Methods From February 2012 to April 2018,the clinical data of 84 children with renal duplex kidney disease admitted to our hospital,were reviewed and analyzed.Among them,75 cases (89.3%) were unilateral and 9 cases (10.7%) were bilateral.According to the preoperative CT + CTA and the duplicated renal morphology,size,location,and anatomical relationship of the kidney segment,combined with the branches and passage of the renal artery which supplies the upper moiety,it described the blood supply of the upper renal moiety.At the same time,it analyzed various types of embryological related factors and gender differences and summarized the tips for laparoscopic surgery.Results Of the 93 duplex kidneys,69 (74.2%) were supplied with 1 artery,and 25 (26.9%) were supplied with 2 or more arteries.Based on the shape and orifice of artery,they were divided into 3 types.The most common type was that the renal artery separated into two or more arteries near the renal parenchyma.The upper and lower renal poles were respectively supplied,which could be summarized as early branching,a total of 71 sides (76.3 %).The second type was the arteries from abdominal aorta or its branches,directly flowing into the upper renal pole,which was classified into the sub-renal artery,a total of 18 sides (19.4%).The others (4 sides,3.3%) were less common,and most of them were a combination of the above-mentioned two types,and one of them whose upper pole was supplied by branches of adrenal artery.Based on the classification of vascular variability,no significant difference was found between males and fenmals,or left and right sides.Conclusions The upper renal moiety are mainly supplied by one branch of renal artery,and the most common type of this artery is prehilar branch,without gender difference.The determination of vascular variability before surgery can avoid bleeding during surgery and avoid accidental injury of normal blood vessels.
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BACKGROUND: Infection of orthopedic implants is a problem to be solved. Currently, scholars are trying to endow orthopedic implants with an anti-infection function, and the research on antibacterial activity of implants has been a hot topic. OBJECTIVE: To introduce the modification methods of orthopedic metal implants in recent years. METHODS: Science Direct online (2015-2019) , WanFang (2015-2018) and VIP (2015-2018) databases were retrieved for literatures using the keywords of "antimicrobial, coating" in English and Chinese, respectively. RESULTS AND CONCLUSION: The currently used antimicrobial modification for orthopedic implants mainly consists of the self-antibacterial modification of the implant and the antibacterial modification on the surface of the implant. The antibacterial modification methods on the surface of the implant mainly include electrochemical modification method, magnetron sputtering method, spraying method, chemical conversion method, laser surface modification method, plasma injection method, nanosphere photolithography, sol/gel method, hydrothermal method, layer-by-layer assembly and combined treatment of various methods. Each antimicrobial modification method has its own merits and demerits.
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Objective To study the range of central venous pressure (CVP) in very low birth weight (VLBW) infants within the first week after birth.Method From February 2014 to February 2018,50 VLBW infants without serious diseases during the first 7 days of life received umbilical venous catheters were prospectively enrolled.CVPs were measured every 4~6 h.The trend of CVP and the correlation of CVP (within 24 h) and birth weight,gestational age were analyzed.Result A total of 50 VLBW infants and 1 291 CVP measurements were included.The CVP increased slightly within 48 h after birth,and then declined.The 95%CI of CVPs were 3.67~4.21,4.03~4.49,3.90~4.33,3.67~4.19,3.29~3.97,3.14~3.94 and 2.64~ 3.55 cmH2O from day 1 to day 7.No significant correlation existed between CVP in the first day and birth weight,nor gestational age (r=-0.267,P=0.073;r=0.106,P=0.762).Conclusion The CVP of VLBW infants increased slightly within 48 h after birth,and then declined.There was no significant correlation between CVP in the first day and birth weight,nor CVP and gestational age.
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Emotional disorder is one of the main clinical symptoms of autism spectrum disorder(ASD),which is related to the lack of emotional awareness,social cognition and alexithymia. It is often manifested as difficulty in recognizing,distinguishing and processing emotional information of oneself and others,resulting in making inappropriate emotional reactions. Children and adolescents with ASD often participate in school bullying as the perpetrator,victim,or victim-perpetrator,and this brings about a series of negative physical and mental effects on them. Bullying prevention should not only include interventions for core symptoms and comorbidities of ASD,but also involve building partner empathy and social skills,teacher responsibility and supportive services,parental involvement,and social integration.
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The present study investigated the protective actions of telmisartan, an angiotensin II type 1 receptor blocker (ARBs), against the cell apoptosis induced by exposure to hydrogen peroxide (H2O2) in differentiated PC12 cells. Preincubation of PC12 cells with telmisartan prevented H2O2-induced cytotoxicity as indicated by increased MTT (3,(4,5-dimethylthiazole-2-yl)2,5-diphenyl-tetrazolium bromide) reduction, decreased lactate dehydrogenase (LDH) release, and improved morphological changes. Hoechst 33,258 staining showed that telmisartan markedly reduced shrunken nuclei of the cells, and Western blot analysis indicated that telmisartan significantly attenuated caspase-3 activity, as indicated by decreased ratio of cleaved Caspase-3 to its precursor and increased ratio of Bcl-2/Bax. The present findings showed that telmisartan protected against cellular oxidative damages by inhibiting apoptotic response.
Subject(s)
Angiotensin II Type 1 Receptor Blockers/pharmacology , Apoptosis/drug effects , Hydrogen Peroxide/pharmacology , Oxidative Stress/drug effects , Telmisartan/pharmacology , Animals , Cell Differentiation/drug effects , PC12 Cells , RatsABSTRACT
Objective To explore the validity of lead aVL in combination with lead V1 for identifying idiopathic outflow tract premature ventricular contractions(PVCs)originating from aortic sinus cusp(ASC).Methods This study consecutively enrolled 102 idiopathic outflow tract PVCs patients who underwent radiofrequency catheter ablation at the Second Xiangya Hospital,Central South University between January 2015 and August 2017.We compared the QRS wave amplitudes in the surface twelve leads electrocardiography between PVCs originating from ASC and right ventricular outflow tract(RVOT).Results(1)The origin sites of PVCs were ASC(n=28,27.5%)and RVOT(n=74,72.5%).The lead V1R/S wave amplitude ratio and lead aVL S wave amplitude were significantly higher in the ASC group than in the RVOT group[(1.14±1.32)vs.(0.16±0.18),P<0.001;(0.99±0.36)mV vs.(0.56±0.26)mV,P<0.001].The areas under the receiver operating characteristic curve(AUCs)and 95%confidence intervals of V1R/S wave amplitude ratio and aVL S wave amplitude had relatively larger AUCs which were 0.894(0.824-0.964)and 0.831(0.749-0.912),with the cut-offs of 0.25 and 0.80 respectively.(2)The sensitivity,specificity and accuracy of the lead V1R/S wave amplitude ratio>0.25 to identify ASC originating PVCs were 78.9%,83.7%and 82.4%,respectively.The sensitivity,specificity and accuracy of the lead aVL S wave amplitude>0.80 mV were 78.6%,85.1%and 83.3%,respectively.The lead aVL S wave amplitude>0.80 mV in combination with the lead V1R/S wave amplitude ratio>0.25 was applied to developed a new diagnostic approach and the sensitivity,specificity and accuracy were 60.7%,93.2%and 84.3%,respectively.Conclusions Lead aVL in combination with lead V1 could be applied to develop a more accurate method for identifying ASC originating PVCs.
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Objective To establish a rapid,high-efficiency and clean way to monitor and pretreat the radioactivity in foods.Methods Food samples,20 kg each of cabbage,crucian,shrimp,pork and 20 L of milk,were collected around Qinshan nuclear power plant,totaling 10 samples for each food.Each type of foods was arranged in two groups each with 10 samples.Samples were pretreated using traditional ashing method (TAM) and microwave ashing method (WAM) separately.HDEHP were used for 90Sr separation and measurement.Calculations were made for the time spent on food pretreatment.The ash-fresh ratio and the recovery rate of 90Sr by the two different method were calculated,with the quality of ash being evaluated.Results The mean time spent on pretreatment was cabbage 20 h,crucian 54 h,shrimp 46 h,pork 58 h and milk 74 h for TAM group,and cabbage 8.5 h,crucian 26 h,shrimp 23 h,pork 26 h and milk 30 h for WAM group,respectively.The time spent by WAM was half the time less than by TAM,especially for animal edible product.The ash-fresh ratios from WAM group were higher than from TAM group.The recovery rates of 90Sr from WAM group in the analytical process were all above 75%,higher than from TAM group.Ashes produced by WAM were hoary,uniform,carbon residue-free and fully ashed.Conclusions WAM pretreatment can pretreat food rapidly,efficiently,cleanly with less loss and better ashing effect.These can meet the requirement of rapid pretreatment of radioactivity in food.
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Objective To study the effect of comprehensive prehospital management for patients with emergency trauma treatment. Methods Fifty emergency patients who were admitted to the emergency department were investigated respectively before and after the implementation of the comprehensive prehospital management of the people's hospital of Gaozhou City, with the service satisfaction questionnaire, the Anxiety Self-assessment Scale (SAS) and the Depression Self-rating Scale (SDS) . The time of admission, first aid time, emergency treatment effect, satisfaction and mental status change of the two groups were compared. Results There was no statistically significant difference in age, gender, cause of injury and distribution of injury in the two groups (P>0.05) . The admission time and first aid time of the patients in the observation group respectively were (15.6±4.2) and (33.5±8.4) minutes, which were shorter than the control group of (24.3±5.0) and (60.5±10.2) minutes (P<0.05) . The emergency effect and satisfaction of the observation group were superior to the control group (P<0.05) . The difference between SAS and SDS scores in the observation group was 21.3 ±3.4 and 20.4±3.1, both of them were higher than the control group (12.1±2.7 and 12.8±2.5) (P<0.05) . Conclusion The comprehensive pre-hospital management can shorten the time of admission and first aid, and effectively improve the clinical treatment effect and satisfaction of emergency trauma patients.