ABSTRACT
Bioenergy is a crucial element of the future energy system with wide range of applications in electricity, heat and transport. A major challenge for the analysis and optimisation of the bioenergy system is the degree of diversity and complexity compared to wind or solar energy. A coherent database for studying the role of bioenergy in the energy system needs to cover the different entities such as bio-resources, conversion procedures and process chains. Since there is no comprehensive data collection for bioenergy so far, we develop a SQLite database by merging several existing datasets and additional information. The resulting Bio-Energy Technology Database (BET.db) provides a consistent set of 141 feedstocks as well as energy carriers, 259 conversion technologies, and 134 energy supply concepts. The proof of concept within a bioenergy system modelling a wide range of technologies for the electricity, heat and transport sectors using the BENOPT model has been successful. By providing a one-stop-shop solution for techno-economic information about on the bioenergy nexus, this blind spot can be avoided for further investigations. The current stage of development is an intermediate prototype that will be developed into a more versatile and interactive web application later on.
ABSTRACT
*Type II secretion (T2S) systems of many plant-pathogenic bacteria often secrete cell wall-degrading enzymes into the plant apoplast. *Here, we show that the Xps-T2S system from the plant pathogen Xanthomonas campestris pv vesicatoria (Xcv) promotes disease and contributes to the translocation of effector proteins that are delivered into the plant cell by the type III secretion (T3S) system. *The Xcs-T2S system instead lacks an obvious virulence function. However, individual xcs genes can partially complement mutants in homologous xps genes, indicating that they encode functional components of T2S systems. Enzyme activity assays showed that the Xps system contributes to secretion of proteases and xylanases. We identified the virulence-associated xylanase XynC as a substrate of the Xps system. However, homologs of known T2S substrates from other Xanthomonas spp. are not secreted by the T2S systems from Xcv. Thus, T2S systems from Xanthomonas spp. appear to differ significantly in their substrate specificities. *Transcript analyses revealed that expression of xps genes in Xcv is activated by HrpG and HrpX, key regulators of the T3S system. By contrast, expression of xynC and extracellular protease and xylanase activities are repressed by HrpG and HrpX, suggesting that components and substrates of the Xps system are differentially regulated.