ABSTRACT
A novel actinobacterium, designated strain YIM 75704T, was isolated from a limestone quarry located at Gulbarga, Karnataka, India. The novel strain has showed typical morphological and chemotaxonomic characteristics of the family Streptomycetaceae. Comparison of 16S rRNA gene sequences indicated that this strain represents a novel member of the family Streptomycetaceae and exhibited 99.0% 16S rRNA gene sequence similarities with the type species of the recently described novel genus Allostreptomyces, that is, Allostreptomyces psammosilenae, whereas other species of Streptomyces were below 95% sequence similarity. The cell hydrolysates contained the LL-isomer of diaminopimelic acid and the predominant quinones were MK-9 (H6, H8 and H4). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylinositolmannosides and three unknown phospholipids. The DNA G+C content was 75.0 mol%. A polyphasic study of the strain with morphological, phenotypic, phylogenetic and with DNA-DNA hybridization evidence with related members showed that this strain represents novel species of Allostreptomyces for which the name Allostreptomyces indica sp. nov., is proposed. The type strain is YIM 75704T (=âDSM 41985T=CCTCC AA 209051T= NCIM 5485T).
Subject(s)
Environmental Microbiology , Streptomycetaceae/classification , Streptomycetaceae/isolation & purification , Base Composition , Cell Wall/chemistry , Cluster Analysis , Cytosol/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Diaminopimelic Acid/analysis , India , Nucleic Acid Hybridization , Phospholipids/analysis , Phylogeny , Quinones/analysis , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Streptomycetaceae/geneticsABSTRACT
A complex of Ag/AgCl nanoparticles was synthesized on the basis of the extracellular polysaccharide of Azotobacter chroococcum XU1 and 10 mM AgNO3 solution. The complex was characterized by UV spectroscopy, X-ray diffraction, and scanning electron microscopy. Colloidal solutions of the complex had absorption peaks at 260 and 420 nm, indicating the formation Ag/AgCl nanoparticles. The size of the nanoparticles varied from 6 to 50 nm. The nanobiocomposite consisting of the exopolysaccharide matrix and Ag/AgCl nanoparticles exhibited a fungicidal effect against such plant pathogens as Fusarium oxysporum f. sp. vasinfectum and Verticillium dahliae.
Subject(s)
Azotobacter/chemistry , Nanoparticles/chemistry , Polysaccharides, Bacterial/chemistry , Silver Compounds/chemistry , Silver/chemistryABSTRACT
The semi-private property rights arrangement called the Household Production Responsibility System (HPRS) was started in the early 1980s in Xilingol pasture of Inner Mongolia (China), and stimulated the development of stockbreeding. The grassland has been degrading severely with increasing numbers of livestock. Based on a historical review of property rights regimes in Inner Mongolia and empirical surveys in Xilingol pasture during 2001-2003, this paper assesses the implementation of HPRS and its impacts on incomes of households as well as the environmental impact on the grassland. It was found that HPRS does not mitigate the "Tragedy of the Commons", instead it has exacerbated the situation. It was also found that co-management of grassland and livestock among a few households presents a sustainable use of grassland to develop livestock breeding. We conclude with the recommendation that small-scale collective property rights systems should be encouraged in Xilingol pasture of Inner Mongolia.
