ABSTRACT
As research on parasitic helminths is moving into the post-genomic era, an enormous effort is directed towards deciphering gene function and to achieve gene annotation. The sequences that are available in public databases undoubtedly hold information that can be utilized for new interventions and control but the exploitation of these resources has until recently remained difficult. Only now, with the emergence of methods to genetically manipulate and transform parasitic worms will it be possible to gain a comprehensive understanding of the molecular mechanisms involved in nutrition, metabolism, developmental switches/maturation and interaction with the host immune system. This review focuses on functional genomics approaches in parasitic helminths that are currently used, to highlight potential applications of these technologies in the areas of cell biology, systems biology and immunobiology of parasitic helminths.
Subject(s)
Genome, Helminth , Genomics/methods , Helminths/genetics , Animals , Female , Gene Transfer Techniques , Helminth Proteins/genetics , Helminthiasis/parasitology , Helminths/classification , Helminths/physiology , Humans , Male , RNA InterferenceABSTRACT
Sarcoptic mange (or scabies) is an important skin disease which can affect a variety of species including humans, cattle, goats, sheep, horses, pigs, rabbits, and dogs. Approximately 300 million people are affected worldwide and in lifestock animals the infestation may lead to substantial economic losses caused by depression in growth and feed conversion rates. Diagnosis of Sarcoptes infestation is difficult and only a few serological tests have been developed using whole mite antigen for diagnosis of mange in animals. Here we describe the isolation and characterisation of cDNAs of several immunoreactive clones and their recombinant expression in Escherichia coli. Three of the proteins contain repetitive sequences which suggests that they might be involved in immune evasion. The application of these antigens in serodiagnosis and the suitability for diagnosis is discussed.
Subject(s)
Antigens/immunology , Enzyme-Linked Immunosorbent Assay/veterinary , Sarcoptes scabiei/genetics , Sarcoptes scabiei/immunology , Scabies/veterinary , Amino Acid Sequence , Animals , Base Sequence , Cloning, Molecular , DNA, Complementary/chemistry , DNA, Complementary/genetics , Enzyme-Linked Immunosorbent Assay/methods , Enzyme-Linked Immunosorbent Assay/standards , Escherichia coli , Gene Library , Molecular Sequence Data , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/veterinary , Reproducibility of Results , Scabies/diagnosis , Sensitivity and Specificity , Sequence Alignment/veterinary , Serologic Tests/methods , Serologic Tests/standards , Serologic Tests/veterinaryABSTRACT
Intracellular bacteria of the genus Wolbachia are found in most filarial nematodes, but are lacking in some species like Acanthocheilonema viteae. Due to their symbiotic nature and their role in the pathology of filarial infections they are considered to be potential targets for intervention against filarial infections in man. Infection of A. viteae (a species which does not naturally carry Wolbachia) with Wolbachia bacteria could allow comparative studies on the effect of the endobacterium on the parasite and on the host's immune systems. As a step towards such studies we microinjected adult female A. viteae with Wolbachia obtained from Litomosoides sigmodontis. The bacteria were isolated from L. sigmodontis by density-gradient centrifugation, microinjected into A. viteae worms and bacterial DNA detected by PCR with Wolbachia specific primers (ftsZ gene). Microinjected worms were cultured in vitro, and 81% survived for 10 days. Implantation of microinjected worms into Meriones unguiculatus, the rodent host of A. viteae resulted in 38% survival. The DNA of the microinjected worms recovered from jirds 8 weeks after implantation contained Wolbachia DNA as shown by PCR, suggesting that Wolbachia of L. sigmodontis can be horizontally transmitted to A. viteae.
Subject(s)
Dipetalonema/microbiology , Filarioidea/microbiology , Wolbachia/physiology , Animals , DNA, Bacterial/analysis , DNA, Helminth/analysis , Dipetalonema/genetics , Dipetalonema/ultrastructure , Filarioidea/ultrastructure , Gerbillinae , Microinjections , Ornithodoros , Polymerase Chain Reaction , Symbiosis , Wolbachia/genetics , Wolbachia/ultrastructureABSTRACT
A primary vaccine candidate antigen against schistosomiasis is paramyosin (pmy), a myofibrillar protein found exclusively in invertebrates. Here we report the results of vaccine trials against the Asian schistosome undertaken on inbred and outbred mice and water buffaloes using a bacterially expressed and purified form of Schistosoma japonicum pmy (rec-Sj-97). Vaccination of the mice resulted in high levels of specific anti-pmy IgG antibodies when compared with adjuvant controls and significant reduction in worm burdens and in liver eggs. Furthermore, a significant reduction in liver eggs was recorded in two of the three water buffalo vaccine trials undertaken and, in all three trials, high levels of specific anti-pmy IgG antibodies were generated. There was no evidence of any toxic effects and the vaccine preparations and Quil A adjuvant were clearly well tolerated. The development of a vaccine intended for livestock animals such as bovines would be beneficial in two ways; directly by blocking transmission of schistosomiasis to humans and economically by contributing to healthier livestock. We are encouraged by the consistent efficacy in the mouse and the buffalo vaccine trials that resulted in a significant decrease in liver eggs. Indeed, predictions from mathematical models indicate that an egg reduction effect of 42-45% in buffaloes would be sufficient when combined with human treatment to control schistosomiasis japonica in the marshes and lakes along the middle and upper reaches of the Yangtze River, the most highly endemic areas for the disease in China.
Subject(s)
Schistosoma japonicum/immunology , Schistosomiasis japonica/prevention & control , Tropomyosin/immunology , Vaccines, Synthetic/pharmacology , Animals , Antibodies, Helminth/blood , Antigens, Helminth/genetics , Buffaloes , China , Female , Humans , Immunoglobulin G/blood , Liver/parasitology , Male , Mice , Mice, Inbred CBA , Parasite Egg Count , Schistosoma japonicum/genetics , Schistosoma japonicum/isolation & purification , Schistosomiasis japonica/immunology , Tropomyosin/genetics , Vaccines, Synthetic/genetics , Vaccines, Synthetic/immunologyABSTRACT
Recombinant glyceraldehyde-3-phosphate dehydrogenase of the cestode parasite Echinococcus multilocularis was expressed in Escherichia coli and in Salmonella typhimurium. The potential of different forms of the recombinant antigen to protect BALB/c mice against oral challenge infections with E. multilocularis eggs was evaluated. Oral or intraperitoneal immunisation with live attenuated S. typhimurium as a carrier for recombinant glyceraldehyde-3-phosphate dehydrogenase of the E. multilocularis resulted in significant protection, reducing the number of developing metacestodes up to 79.8%. The sera of protected animals did not contain detectable amounts of antibody against glyceraldehyde-3-phosphate dehydrogenase of E. multilocularis. By contrast, although anti-glyceraldehyde-3-phosphate dehydrogenase of E. multilocularis antibodies were detectable in the sera, immunisation with E. coli-expressed recombinant glutathione-S-transferase-fusion protein or with glyceraldehyde-3-phosphate dehydrogenase of E. multilocularis fused to a 6HIS-tag failed to protect the animals against oral challenge infections. These data emphasise that antigen delivery systems play a critical role in vaccination and the induction of protective immunity against helminth parasites.
Subject(s)
Echinococcosis/immunology , Echinococcus/immunology , Glyceraldehyde-3-Phosphate Dehydrogenases/immunology , Immunization , Amino Acid Sequence , Animals , Base Sequence , Blotting, Western , Cloning, Molecular , Echinococcosis/parasitology , Echinococcosis/prevention & control , Echinococcus/enzymology , Escherichia coli/enzymology , Escherichia coli/genetics , Female , Glyceraldehyde-3-Phosphate Dehydrogenases/biosynthesis , Glyceraldehyde-3-Phosphate Dehydrogenases/genetics , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Recombinant Proteins/biosynthesis , Recombinant Proteins/genetics , Salmonella typhimurium/enzymology , Salmonella typhimurium/genetics , Sequence Homology, Amino Acid , Statistics, NonparametricABSTRACT
Schistosomes feed on human blood. They employ proteases to degrade hemoglobin from ingested erythrocytes, using the residues released for amino acid metabolism. However, the identity and the role of the participating protease(s) are unclear and controversial. Confocal microscopy localized schistosomal cathepsin D to the parasite gastrodermis, and revealed elevated protease expression in females. At sub-cellular level, cathepsin D was localized to superficial digestive vacuoles of the gut and to cisternae of the gastrodermal rough endoplasmic reticulum. Schistosome cathepsin D, expressed in insect cells, autoactivated at pH 3.6 to a approximately 40 kDa form that cleaved the substrates o-aminobenzoyl-Ile-Glu-Phe-nitroPhe-Arg-leu-NH(2) and hemoglobin. The NH(2)-terminal residues of mature cathepsin D of Schistosoma japonicum and Schistosoma mansoni were Asn1 and Gly1, respectively, revealing that the proregion peptide was comprised of 35 residues. The proteases cleaved hemoglobin at pH 2.5--4.6, releasing numerous fragments. S. Japonicum cathepsin D cleaved at 13 sites, S. mansoni cathepsin D at 15 sites. Early cleavage sites were alpha Phe33-Leu34 and beta Phe41-Phe42, while others included alpha Leu109-Ala-110 and beta Leu14-Trp15, demonstrating a preference for bulky hydrophobic residues at P1 and P1'. Most of the schistosomal cathepsin D cleavage sites were discrete from those of human cathepsin D. The gastrodermal location, elevated expression in females, acidic pH optima, similar substrate preferences in two species, and the discrete substrate preferences compared with human cathepsin D together provide compelling support for the hypothesis that schistosomal cathepsin D plays an integral role in hemoglobin proteolysis, and might be selectively targeted by drugs based on protease inhibition.
Subject(s)
Aspartic Acid Endopeptidases/metabolism , Cathepsin D/metabolism , Hemoglobins/metabolism , Schistosoma japonicum/enzymology , Schistosoma mansoni/enzymology , Amino Acid Sequence , Animals , Cathepsin D/isolation & purification , Female , Fluorescent Antibody Technique , Hemoglobins/chemistry , Humans , Hydrogen-Ion Concentration , Microscopy, Confocal , Stomach/enzymologyABSTRACT
Paramyosin of Schistosoma japonicum was expressed at a high level in E. coli. The recombinant protein could be easily purified from bacteria lysate by fast protein liquid chromatography(FLPC) on a TALON resin column, due to the protein being expressed with a tag of six histidine residue fused to the N-terminus. The protein was completely soluble and could be eluted under non-denaturing condition using imidazole. To eliminate imidazole and residue of E. coli, the elution was further purified by ion-exchange chromatography. The purified protein will be used in water buffaloes in the study on protective immunity against Schistosoma japonicum.
Subject(s)
Antigens, Helminth/genetics , Escherichia coli/genetics , Schistosoma japonicum/genetics , Tropomyosin/genetics , Animals , Antigens, Helminth/biosynthesis , Recombinant Proteins/biosynthesis , Recombinant Proteins/genetics , Schistosoma japonicum/metabolism , Tropomyosin/biosynthesisABSTRACT
Peripheral blood mononuclear cells (PBMC) from 117 individuals living on two islands in an area (Dongting Lake) endemic for schistosomiasis japonica in China, and 15 control individuals from a non-endemic area of China, were assessed for antigen-stimulated proliferation against five recombinant Schistosoma japonicum antigens of recognized interest in the development of immunity to schistosomiasis. Two recombinant antigens, paramyosin and 28-kD glutathione-S-transferase, stimulated cellular proliferation (stimulation index > or = 3.0) in 38.5% and 42.5% of subjects, respectively, a level similar to that induced by a soluble whole parasite extract (51.3%). In contrast, three other recombinant antigens tested--a fatty acid binding protein, 22-kD tegumental membrane-associated antigen, and glyceraldehyde-3-phosphate dehydrogenase--stimulated PBMC proliferation in only 3-8% of subjects. Moreover, we also identified a positive association between the degree of exposure, and cellular proliferation following stimulation with recombinant paramyosin or whole parasite extract. Highly significant differences in antigen-stimulated proliferation were also observed between the two islands, Niangashan and Qingshan. The whole parasite extract stimulated proliferation in 90% of subjects from Niangashan island compared with only 42.1% of subjects from Qingshan island (chi2 = 16.88, P = 0.00004), while glutathione-S-transferase stimulated proliferation in 77.3% of subjects from Niangashan island compared with only 34.7% of subjects from Qingshan island (chi2 = 13.09, P = 0.003). A similar, but not significant, trend was observed for paramyosin and the fatty-acid binding protein. The identification of differential cellular proliferative responses to specific schistosome antigens within an infected human population may have important practical implications for vaccine development against schistosomiasis japonica.
Subject(s)
Antigens, Helminth/immunology , Leukocytes, Mononuclear/immunology , Schistosoma japonicum/immunology , Schistosomiasis japonica/immunology , Adult , Animals , Antigens, Helminth/genetics , Cell Division/immunology , China/epidemiology , Female , Humans , Leukocytes, Mononuclear/pathology , Male , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Schistosomiasis japonica/blood , Schistosomiasis japonica/epidemiologyABSTRACT
Paramyosin from parasitic worms of the genus Schistosoma has shown promise as a vaccine target and it is one of the candidates selected by WHO for the development of a vaccine against schistosomiasis. Here we discuss the literature of the past decade and report on different recombinant paramyosin constructs we are using in our laboratory to develop a vaccine against the Asian schistosoma, Schistosoma japonicum.
Subject(s)
Schistosoma japonicum/immunology , Schistosomiasis japonica/prevention & control , Tropomyosin/immunology , Vaccines, Synthetic/isolation & purification , Amino Acid Sequence , Animals , Antigens, Helminth/genetics , Helminth Proteins/genetics , Helminth Proteins/immunology , Humans , Molecular Sequence Data , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Schistosoma japonicum/genetics , Schistosomiasis japonica/immunology , Tropomyosin/geneticsABSTRACT
Schistosomes acquire amino acids for growth, development, and reproduction by catabolizing hemoglobin obtained from ingested host erythrocytes. While the biochemical pathway(s) involved has not been determined definitively, a number of proteases including schistosome legumain and cathepsin L-, D-, B- and C-like enzymes have been ascribed roles in the degradation of hemoglobin to diffusible peptides. Transcripts encoding these schistosome proteases, which appear to be expressed in the gastrodermis and cecum of the schistosome, have been reported. Because these enzymes are candidate targets at which to direct novel anti-schistosomal therapies, the comparative biochemistry of these and their counterpart mammalian proteases is now the focus of research in a number of laboratories. This paper reviews reports dating from 40 years ago to the present on how schistosomes digest host-derived hemoglobin, and interprets apparent anomalies in some earlier compared to later reports, the latter having benefited from the availability of PCR and gene cloning technologies. More specifically, the review concentrates on five proteolytic enzymes, and their associated genes, which have been ascribed key roles in the pathway of hemoglobin degradation.
Subject(s)
Hemoglobins/metabolism , Peptide Hydrolases/metabolism , Schistosoma/enzymology , Schistosomiasis/enzymology , Schistosomiasis/parasitology , Animals , Host-Parasite Interactions , Humans , Hydrolysis , Molecular Sequence DataABSTRACT
There has been a tremendous explosion in the area of DNA vaccine research over the last 4 years, particularly in relation to antiviral vaccines. This report discusses the development and application of this new technology with regard to parasitic infections. Progress has been made towards the development of a vaccine against malaria, cryptosporidiosis, leishmaniasis, toxoplasmosis and schistosomiasis. In the future, nucleic acid vaccines will be a useful tool to help control these and other parasitic infections.
Subject(s)
Antigens, Protozoan/immunology , Parasitic Diseases/immunology , Parasitic Diseases/prevention & control , Vaccination/methods , Vaccines, DNA/therapeutic use , Animals , Cryptosporidiosis/immunology , Cryptosporidiosis/prevention & control , Forecasting , Leishmaniasis/immunology , Leishmaniasis/prevention & control , Malaria/immunology , Malaria/prevention & control , Mice , Parasitic Diseases, Animal/immunology , Parasitic Diseases, Animal/prevention & control , Schistosomiasis/immunology , Schistosomiasis/prevention & control , Toxoplasmosis/immunology , Toxoplasmosis/prevention & control , Toxoplasmosis, Animal/immunology , Toxoplasmosis, Animal/prevention & controlABSTRACT
We have isolated a cDNA from the hydatid tapeworm, Echinococcus granulosus, encoding a protein that binds laminin. This is the first report of a helminth parasite laminin-binding protein and the first description of a cDNA encoding a laminin-binding protein from a parasite. The cDNA clone (egmo3) was isolated from an E. granulosus protoscolex cDNA expression library, and identified on the basis of sequence homology to the nonintegrin mammalian metastasis-associated 67-kDa laminin receptor (67-LR). The amino acid sequence predicted from the cDNA sequence is 268 residues long with a calculated molecular mass of 29.9 kDa. Southern blot analysis suggested that many copies of the gene may occur in the E. granulosus genome. A Northern blot revealed that the gene is expressed as a single transcript of approximately 1 kb consistent with the size of the cDNA insert. Antibodies raised to the purified protein interacted with a 30 kDa protein in whole E. granulosus protoscoleces. A Western blot of the purified and refolded recombinant protein specifically bound 125I-labelled laminin, as did a synthetic peptide derived from the inferred amino acid sequence of egmo3 which is similar in homology to peptide G, the active ligand-binding site of 67-LR. We also isolated the 3' end of the cDNA encoding the homologous protein from the closely related species, E. multilocularis. The polypeptide encoded by egmo3 also shares substantial identity with the acidic class of ribosomal proteins which are involved in protein synthesis. As such, the egmo3 protein may be multifunctional in E. granulosus, acting as a laminin-binding molecule but also playing a role in cell division and growth.
Subject(s)
DNA, Helminth/genetics , Echinococcus/genetics , Helminth Proteins/genetics , Protein Precursors , Receptors, Laminin/genetics , Amino Acid Sequence , Animals , Binding Sites/genetics , Cattle , Cloning, Molecular , DNA, Complementary/genetics , Echinococcus/metabolism , Gene Expression , Genes, Helminth , Helminth Proteins/metabolism , Humans , Laminin/metabolism , Mice , Molecular Sequence Data , Receptors, Laminin/metabolism , Sequence Homology, Amino AcidABSTRACT
We have examined the efficacy of nucleic acid vaccination in inducing immunity to the multicellular parasite, Schistosoma japonicum, a trematode worm responsible for causing schistosomiasis in humans and other mammalian species. A panel of Schistosoma japonicum cDNAs were cloned into eukaryotic expression vectors, injected into animals, and tested for immunogenicity. The cDNAs tested encoded 26- and 28-kDa glutathione-S-transferases, calreticulin, glyceraldehyde-3-phosphate dehydrogenase, a 22.6 kDa membrane-associated antigen, a 14 kDa fatty-acid binding protein, fragments of paramyosin, full-length paramyosin, and a novel gene comprising the 26 kDa glutathione-S-transferase fused to a fragment of paramyosin cDNA. The paramyosin gene constructs, including the fusion, were all able to induce anti-paramyosin antibodies; with the fragments of paramyosin these were of the IgG1, IgG2a and IgG2b isotypes. In contrast, none of the other schistosome cDNAs tested were able to induce detectable antibody responses. The anti-paramyosin antibodies did not protect mice challenged with cercariae of S. japonicum.
Subject(s)
Antibodies, Helminth/biosynthesis , Schistosoma japonicum/genetics , Schistosomiasis japonica/prevention & control , Vaccines, DNA , Animals , Calcium-Binding Proteins/genetics , Calcium-Binding Proteins/immunology , Calreticulin , Female , Genes, Helminth , Glutathione Transferase/genetics , Glutathione Transferase/immunology , Glyceraldehyde-3-Phosphate Dehydrogenases/genetics , Glyceraldehyde-3-Phosphate Dehydrogenases/immunology , Immunity, Cellular , Mice , Mice, Inbred BALB C , Parasite Egg Count , Rabbits , Rats , Rats, Inbred F344 , Recombinant Fusion Proteins/immunology , Ribonucleoproteins/genetics , Ribonucleoproteins/immunology , Schistosoma japonicum/immunology , Schistosomiasis japonica/immunology , Tropomyosin/genetics , Tropomyosin/immunology , Vaccines, DNA/immunologyABSTRACT
A cDNA encoding the complete open reading frame of the Schistosoma japonicum paramyosin has been constructed and cloned. The 2600 bp cDNA was engineered by PCR using a N-terminally truncated paramyosin clone (pmy25) as template and a 57-mer primer that introduced the eight missing amino acids and matched the 5' sequence of pmy25 in conjunction with a pmy specific reverse primer. After cloning and expression, the recombinant protein was purified by affinity chromatography under non-denaturing conditions and was shown to have a molecular mass of 99 kDa which is equivalent to the expected size of the full length recombinant fusion protein, comprising the 97 kDa paramyosin plus an additional 2 kDa for the N-terminal fusion peptide incorporating the six histidine residues required for purification. In Western blot assays it reacted specifically with anti-paramyosin antibodies in sera from vaccinated animals and patients with Asian schistosomiasis. The engineering of the full-length cDNA encoding Schistosoma japonicum paramyosin, its bacterial expression and purification will facilitate future studies aimed at determining its efficacy as an anti-schistosomiasis vaccine.
Subject(s)
Schistosoma japonicum/genetics , Tropomyosin/genetics , Animals , Blotting, Western , Chromatography, Affinity , DNA, Complementary/genetics , Electrophoresis, Polyacrylamide Gel , Humans , Recombinant Proteins/biosynthesis , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Recombinant Proteins/isolation & purification , Schistosoma japonicum/immunology , Tropomyosin/immunologyABSTRACT
A cDNA encoding the complete open reading frame of the Taenia solium acidic ribosomal phosphoprotein P2 has been cloned. The 417 bp cDNA (arpP2) was isolated from a T. solium cDNA expression library by PCR using P protein specific pimers. The ORF encodes a protein of 121 amino acids exhibiting 40-55% identity to mammalian, fungal, insect, and parasite P2 proteins. The inferred molecular mass of the protein is 12,592 Daltons, similar to that reported for other ribosomal P2 proteins. After subcloning and expression, the recombinant protein was purified by affinity chromatography under non-denaturing conditions and was shown to have a molecular mass of 15 kDa which is equivalent to the expected size of the full length recombinant fusion protein, comprising the 12.5 kDa arpP2 plus an additional 2 kDa for the N-terminal fusion peptide incorporating the six histidine residues required for purification.
Subject(s)
Cysticercosis/parasitology , Phylogeny , Protozoan Proteins , Ribosomal Proteins/biosynthesis , Taenia/metabolism , Amino Acid Sequence , Animals , Artemia/genetics , Babesia bovis/genetics , Base Sequence , Cloning, Molecular , DNA Primers , DNA, Complementary , Dictyostelium/genetics , Drosophila melanogaster/genetics , Fungi/genetics , Gene Expression , Gene Library , Humans , Leishmania infantum/genetics , Molecular Sequence Data , Open Reading Frames , Polymerase Chain Reaction , Rats , Recombinant Proteins/biosynthesis , Restriction Mapping , Ribosomal Proteins/genetics , Sequence Homology, Amino Acid , Taenia/genetics , Taenia/pathogenicityABSTRACT
A cDNA encoding a Schistosoma japonicum aspartic proteinase was cloned, sequenced, and found to encode a zymogen of 380 amino acid residues, and its gene was shown to be present as a single copy in the S. japonicum genome. Identity comparisons showed that the enzyme (Sjpasp) was most closely related to the cathepsin Ds. The deduced amino acid sequence has four potential glycosylation sites, two of which are in identical positions to the two glycosylation sites of human kidney lysosomal cathepsin D. Furthermore, all four disulfide bonds found in mammalian cathepsin D sequences are present in Sjpasp, although the beta-hairpin (loop 3), which is cleaved during maturation of vertebrate cathepsin Ds to yield light and heavy chain subunits, is absent from Sjpasp. While most residues involved in substrate specificity and catalysis of aspartic proteinases are preserved in Sjpasp, several residues in these regions exhibit changes that may result in a novel substrate specificity. Aspartic proteinase activity is present in extracts of adult S. japonicum and Schistosoma mansoni and in culture media in which schistosomes were maintained and was capable of digesting hemoglobin. The schistosome aspartic proteinase may play a pivotal role in the catabolism of hemoglobin obtained from host erythrocytes.
Subject(s)
Aspartic Acid Endopeptidases/genetics , Aspartic Acid Endopeptidases/metabolism , Hemoglobins/metabolism , Phylogeny , Schistosoma japonicum/enzymology , Schistosoma japonicum/genetics , Amino Acid Sequence , Animals , Aspartic Acid Endopeptidases/chemistry , Blotting, Southern , Cathepsin D/chemistry , Cathepsin D/genetics , Cloning, Molecular , DNA, Complementary , DNA, Helminth/chemistry , Genes, Helminth , Glycosylation , Humans , Kidney/enzymology , Lysosomes/enzymology , Molecular Sequence Data , Schistosoma japonicum/classification , Schistosoma mansoni/enzymology , Schistosoma mansoni/genetics , Sequence Homology, Amino Acid , VertebratesABSTRACT
Paramyosin is an integral muscle protein found in many invertebrates including schistosomes, and is considered an important candidate vaccine antigen in schistosomiasis. The characterisation of natural molecular variation in vaccine antigens including paramyosin is important because strain-specific vaccination may be necessary against schistosomiasis japonica. We have isolated partial cDNAs encoding paramyosin from an adult, Chinese strain Schistosoma japonicum gene library. Two of these cDNAs (B6 and Y6) encode the same region of paramyosin but their nucleotide sequences differ at eight positions and their deduced amino acid sequences differ by an arginine/cysteine substitution, demonstrating intrastrain variation in paramyosin. Southern blot analysis of genomic DNA from the Chinese and Philippine strains of S. japonicum demonstrated strain-related RFLPs at the paramyosin locus, and suggested that more than one copy of the paramyosin gene was present in the S. japonicum genome. PCR-based RFLP analysis which exploited restriction site differences between B6 and Y6 showed that paramyosin genotype B6 was much more common in schistosome populations and verified the existence of introns in the paramyosin gene(s) of S. japonicum.
Subject(s)
Genes, Helminth/genetics , Genetic Heterogeneity , Schistosoma japonicum/genetics , Tropomyosin/genetics , Amino Acid Sequence , Animals , Base Sequence , DNA, Complementary/genetics , DNA, Helminth/genetics , Genetic Variation/genetics , Molecular Sequence Data , Polymorphism, Restriction Fragment Length , Restriction Mapping , Sequence Analysis, DNA , Tropomyosin/chemistryABSTRACT
The development of an effective vaccine is recognised as a necessary adjunct to the control of schistosomiasis japonica, a disease affecting several million people in China and the Philippines. Currently, recombinant Schistosoma japonicum molecules are considered most suitable for large scale vaccine production and a number of genes encoding vaccine candidate polypeptides have been cloned and expressed (see Waine et al., 1993a). One of the molecules providing most promise as a vaccine target is paramyosin (Butterworth, 1992), a major structural protein of thick filaments in the muscle of most invertebrates; paramyosin genes have now been cloned from a range of parasitic helminths, including schistosomes (Limberger and McReynolds, 1990; Laclette et al., 1991; Dahmen et al., 1993; Landa et al., 1993; Mühlschlegel et al., 1993, Nara et al., 1994). The cloning and nucleotide sequence of S. Japonicum paramyosin is described.
Subject(s)
Genes, Helminth/genetics , Schistosoma japonicum/genetics , Tropomyosin/genetics , Animals , Base Sequence , Cloning, Molecular , DNA, Complementary/genetics , DNA, Helminth/genetics , Molecular Sequence Data , RNA, Helminth/analysis , RNA, Messenger/analysis , Sequence Analysis, DNA , Tropomyosin/chemistryABSTRACT
We report the gene cloning, molecular characterisation and purification of a 14.7-kDa functionally active recombinant (re) cytoplasmic fatty acid-binding protein (Sj-FABPC) from the Chinese strain of the human bloodfluke Schistosoma japonicum (Sj). As schistosomes are unable to synthesise long chain fatty acids and sterols de novo and must, therefore, take up these lipids from the host, Sj-FABPC is an attractive vaccine and/or drug target. Clone 39 (C39), which contains the entire Sj-FABPC gene, was isolated from a Sj lambda ZAPII cDNA expression library immunoscreened with hyperimmune rabbit serum (HRS) raised against soluble adult Sj proteins. The complete ORF (open reading frame) of Sj-FABPC encodes a protein of 132 amino acids (aa) of 14.7 kDa. The aa sequence of Sj-FABPC exhibits 91% identity to a FABP of S. mansoni (Sm14) and 45% identity to a FABP of Fasciola hepatica (Fh15), putative vaccine candidates for schistosomiasis. Sj-FABPC was subcloned into the QIAexpress vector, pQE-10, and subsequently expressed in Escherichia coli. The re-Sj-FABPC, purified under non-denaturing conditions, was recognized by sera from patients with acute and chronic schistosomiasis japonica. The purified re-Sj-FABPC was also shown to bind to palmitic acid with high affinity. The functional expression of Sj-FABPC will facilitate studies on re-Sj-FABPC to assess its potential as a drug and/or vaccine candidate.